################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccI AccIII AclI AflII AhdI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbrPI BciVI BfrI BglII BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I EcoO65I EcoRI EcoRV HpaI MamI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 1480 GAATTT [GA]^AATT[CT] AflIII 1196 ACGTGT A^C[GA][CT]GT AgeI 3557 ACCGGT A^CCGGT Alw44I 1075 GTGCAC G^TGCAC AocI 3564 CCTCAGG CC^T[ACGT]AGG ApaLI 1075 GTGCAC G^TGCAC ApoI 1480 GAATTT [GA]^AATT[CT] AviII 3074 TGCGCA TGC^GCA BbeI 31 GGCGCC GGCGC^C BcgI 239 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2474 TGATCA T^GATCA BsaAI 3209 CACGTA [CT]AC^GT[GA] BsaI 75 GGTCTC GGTCTC(1/5) BsiEI 2853 CGGCCG CG[GA][CT]^CG BsmBI 2708 GAGACG CGTCTC(1/5) Bsp1407I 1964 TGTACA T^GTACA BsrGI 1964 TGTACA T^GTACA BssSI 250 CACGAG CACGAG(-5/-1) Bsu36I 3564 CCTCAGG CC^T[ACGT]AGG DraIII 1258 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 592 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2853 CGGCCG C^GGCCG EclXI 2853 CGGCCG C^GGCCG Eco52I 2853 CGGCCG C^GGCCG Eco81I 3564 CCTCAGG CC^T[ACGT]AGG EcoT22I 2728 ATGCAT ATGCA^T FbaI 2474 TGATCA T^GATCA FseI 2016 GGCCGGCC GGCCGG^CC FspI 3074 TGCGCA TGC^GCA HindIII 3178 AAGCTT A^AGCTT KasI 31 GGCGCC G^GCGCC KspI 293 CCGCGG CCGC^GG NarI 31 GGCGCC GG^CGCC NdeI 2058 CATATG CA^TATG NsiI 2728 ATGCAT ATGCA^T PinAI 3557 ACCGGT A^CCGGT SacII 293 CCGCGG CCGC^GG ScaI 1181 AGTACT AGT^ACT SfoI 31 GGCGCC GGC^GCC SmaI 1758 CCCGGG CCC^GGG SspBI 1964 TGTACA T^GTACA StuI 2502 AGGCCT AGG^CCT TfiI 3633 GATTC G^A[AT]TC XmaCI 1758 CCCGGG C^CCGGG XmaI 1758 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1715 GGTACC G^GTACC 1909 GGTACC AcyI 31 GGCGCC G[GA]^CG[CT]C 2710 GACGCC Aor51HI 2888 AGCGCT AGC^GCT 3648 AGCGCT Asp718I 1715 GGTACC G^GTACC 1909 GGTACC BbsI 1441 GAAGAC GAAGAC(2/6) 1582 GAAGAC BfaI 1738 CTAG C^TAG 2149 CTAG BlpI 909 GCTAAGC GC^T[ACGT]AGC 2884 GCTGAGC Bpu1102I 909 GCTAAGC GC^T[ACGT]AGC 2884 GCTGAGC BpuAI 1441 GAAGAC GAAGAC(2/6) 1582 GAAGAC BsaHI 31 GGCGCC G[GA]^CG[CT]C 2710 GACGCC BsaWI 1718 ACCGGA [AT]^CCGG[AT] 3557 ACCGGT BsrBI 1234 CCGCTC CCGCTC(-3/-3) 574 GAGCGG BstXI 2199 CCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2807 CCACTACTGTGG BstYI 1392 GGATCT [GA]^GATC[CT] 3069 GGATCT CelII 909 GCTAAGC GC^T[ACGT]AGC 2884 GCTGAGC Eco47III 2888 AGCGCT AGC^GCT 3648 AGCGCT Hin1I 31 GGCGCC G[GA]^CG[CT]C 2710 GACGCC KpnI 1715 GGTACC GGTAC^C 1909 GGTACC MaeI 1738 CTAG C^TAG 2149 CTAG MflI 1392 GGATCT [GA]^GATC[CT] 3069 GGATCT MseI 1484 TTAA T^TAA 2906 TTAA PaeR7I 237 CTCGAG C^TCGAG 1118 CTCGAG SbfI 2334 CCTGCAGG CCTGCA^GG 3063 CCTGCAGG SexAI 3416 ACCTGGT A^CC[AT]GGT 3478 ACCAGGT Sse8387I 2334 CCTGCAGG CCTGCA^GG 3063 CCTGCAGG Tru9I 1484 TTAA T^TAA 2906 TTAA XhoI 237 CTCGAG C^TCGAG 1118 CTCGAG XhoII 1392 GGATCT [GA]^GATC[CT] 3069 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI