(Circular) (Six-base) MAPSORT of: pFN21AE1087.seq Check: 1462 from: 1 to: 8422 pFN21AE1087 8422 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2331 2331 Size: 8422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6232 278 Size: 53 83 186 936 4696 2468 Fragments arranged by size: 4696 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5765 1937 Size: 3828 4594 Acc65I G'GTAC_C Cuts at: 3720 3914 5743 3720 Size: 194 1829 6399 Fragments arranged by size: 6399 1829 194 AclI AA'CG_TT Cuts at: 1563 6549 6922 1563 Size: 4986 373 3063 Fragments arranged by size: 4986 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3680 6485 7869 8137 1632 Size: 2048 2805 1384 268 1917 Fragments arranged by size: 2805 2048 1917 1384 268 AfeI AGC'GCT Cuts at: 4895 5655 4895 Size: 760 7662 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8225 AflIII A'CryG_T Cuts at: 1203 3201 7321 8298 1203 Size: 1998 4120 977 1327 Fragments arranged by size: 4120 1998 1327 977 AgeI A'CCGG_T Cuts at: 5562 7253 5562 Size: 1691 6731 AhdI GACnn_n'nnGTC Cuts at: 7151 7151 Size: 8422 AleI CACnn'nnGTG Cuts at: 1166 4661 4817 1166 Size: 3495 156 4771 Fragments arranged by size: 4771 3495 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3207 4902 1184 Size: 2023 1695 4704 Fragments arranged by size: 4704 2023 1695 AlwNI CAG_nnn'CTG Cuts at: 2446 2686 2851 3178 3658 4006 5077 6165 7737 Size: 240 165 327 480 348 1071 1088 1572 Cuts at: 7737 2446 Size: 3131 Fragments arranged by size: 3131 1572 1088 1071 480 348 327 240 165 ApaI G_GGCC'C Cuts at: 2587 3565 3770 4371 2587 Size: 978 205 601 6638 Fragments arranged by size: 6638 978 601 205 ApaLI G'TGCA_C Cuts at: 3080 6479 7635 3080 Size: 3399 1156 3867 Fragments arranged by size: 3867 3399 1156 ApoI r'AATT_y Cuts at: 1493 3485 5731 5957 6109 8218 1493 Size: 1992 2246 226 152 2109 1697 Fragments arranged by size: 2246 2109 1992 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6976 160 Size: 872 5944 1606 Fragments arranged by size: 5944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8422 AvaI C'yCGr_G Cuts at: 1870 1943 2242 2543 2573 3123 3763 5747 1870 Size: 73 299 301 30 550 640 1984 4545 Fragments arranged by size: 4545 1984 640 550 301 299 73 30 BamHI G'GATC_C Cuts at: 5752 6119 5752 Size: 367 8055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2759 3143 3720 3874 Size: 325 217 627 249 723 384 577 154 Cuts at: 3874 3914 4334 4358 4485 4547 5565 5743 7198 Size: 40 420 24 127 62 1018 178 1455 Cuts at: 7198 618 Size: 1842 Fragments arranged by size: 1842 1455 1018 723 627 577 420 384 325 249 217 178 154 127 62 40 24 BanII G_rGCy'C Cuts at: 1391 2340 2587 2833 3369 3565 3678 3770 4371 Size: 949 247 246 536 196 113 92 601 Cuts at: 4371 4575 5251 5259 5315 5741 1391 Size: 204 676 8 56 426 4072 Fragments arranged by size: 4072 949 676 601 536 426 247 246 204 196 113 92 56 8 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 8422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3453 3594 928 Size: 971 1554 141 5756 Fragments arranged by size: 5756 1554 971 141 BbvCI CC'TCA_GC Cuts at: 2172 3779 4107 2172 Size: 1607 328 6487 Fragments arranged by size: 6487 1607 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2233 2267 6613 6647 1903 Size: 34 296 34 4346 34 3678 Fragments arranged by size: 4346 3678 296 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6314 7524 6314 Size: 1210 7212 BclI T'GATC_A Cuts at: 1548 4479 1548 Size: 2931 5491 BfrBI ATG'CAT Cuts at: 4735 4735 Size: 8422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3688 3883 4028 Size: 107 122 71 631 605 2016 195 145 Cuts at: 4028 4579 7033 136 Size: 551 2454 1525 Fragments arranged by size: 2454 2016 1525 631 605 551 195 145 122 107 71 BglII A'GATC_T Cuts at: 8417 8417 Size: 8422 BlpI GC'TnA_GC Cuts at: 2915 4890 5839 2915 Size: 1975 949 5498 Fragments arranged by size: 5498 1975 949 Bme1580I G_kGCm'C Cuts at: 1790 2361 2587 2811 3084 3146 3196 3356 3565 Size: 571 226 224 273 62 50 160 209 Cuts at: 3565 3770 4371 4790 5162 6483 7639 1790 Size: 205 601 419 372 1321 1156 2573 Fragments arranged by size: 2573 1321 1156 601 571 419 372 273 226 224 209 205 160 62 50 BmgBI CAC'GTC Cuts at: 1318 2371 2599 3202 4228 1318 Size: 1053 228 603 1026 5512 Fragments arranged by size: 5512 1053 1026 603 228 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3260 3334 5030 Size: 416 53 83 399 24 1843 74 1696 Cuts at: 5030 5521 5625 6153 7111 442 Size: 491 104 528 958 1753 Fragments arranged by size: 1843 1753 1696 958 528 491 416 399 104 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8422 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2719 2719 Size: 8422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2068 2477 2765 3308 3353 3488 3944 Size: 802 27 409 288 543 45 135 456 Cuts at: 3944 4426 5273 7082 1239 Size: 482 847 1809 2579 Fragments arranged by size: 2579 1809 847 802 543 482 456 409 288 135 45 27 Bpu10I CC'TnA_GC Cuts at: 2172 3779 4107 2172 Size: 1607 328 6487 Fragments arranged by size: 6487 1607 328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5207 6547 7412 7710 5207 Size: 1340 865 298 5919 Fragments arranged by size: 5919 1340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2086 7085 882 Size: 617 587 4999 2219 Fragments arranged by size: 4999 2219 617 587 BsaAI yAC'GTr Cuts at: 493 1204 5216 6213 493 Size: 711 4012 997 2702 Fragments arranged by size: 4012 2702 997 711 BsaBI GATnn'nnATC Cuts at: 8416 8416 Size: 8422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 4716 6229 6611 Size: 53 83 186 936 504 2679 1513 382 Cuts at: 6611 7246 275 Size: 635 1451 Fragments arranged by size: 2679 1513 1451 936 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3723 5562 6855 7253 7527 7674 8085 3723 Size: 1839 1293 398 274 147 411 4060 Fragments arranged by size: 4060 1839 1293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3602 3632 1178 Size: 30 165 30 2199 30 5968 Fragments arranged by size: 5968 2199 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 3377 3623 3626 3629 3767 4049 5447 Size: 1405 786 246 3 3 138 282 1398 Cuts at: 5447 1186 Size: 4161 Fragments arranged by size: 4161 1405 1398 786 282 246 138 3 3 BseYI C'CCAG_C Cuts at: 2076 2108 2484 3022 3557 3693 3770 3861 3932 Size: 32 376 538 535 136 77 91 71 Cuts at: 3932 4188 4193 4874 4950 5227 5506 7625 8121 Size: 256 5 681 76 277 279 2119 496 Cuts at: 8121 2076 Size: 2377 Fragments arranged by size: 2377 2119 681 538 535 496 376 279 277 256 136 91 77 76 71 32 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2695 3824 4658 4726 5568 1163 Size: 573 501 458 1129 834 68 842 4017 Fragments arranged by size: 4017 1129 842 834 573 501 458 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4861 5872 6633 7661 1540 Size: 275 186 2860 1011 761 1028 2301 Fragments arranged by size: 2860 2301 1028 1011 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 2833 3084 3369 3678 4378 5315 6483 6568 Size: 511 251 285 309 700 937 1168 85 Cuts at: 6568 7639 2322 Size: 1071 3105 Fragments arranged by size: 3105 1168 1071 937 700 511 309 285 251 85 BsmI GAATG_Cn' Cuts at: 5933 6026 5933 Size: 93 8329 BsmBI CGTCTCn'nnnn_ Cuts at: 4707 4707 Size: 8422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2340 2361 2587 2811 2833 3084 Size: 399 532 18 21 226 224 22 251 Cuts at: 3084 3146 3196 3356 3369 3565 3678 3770 4371 Size: 62 50 160 13 196 113 92 601 Cuts at: 4371 4378 4575 4790 5162 5251 5259 5315 5741 Size: 7 197 215 372 89 8 56 426 Cuts at: 5741 6483 6568 7639 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 532 426 399 372 251 226 224 215 197 196 160 113 92 89 85 62 56 50 22 21 18 13 8 7 BspEI T'CCGG_A Cuts at: 8085 8085 Size: 8422 BspHI T'CATG_A Cuts at: 6311 8041 6311 Size: 1730 6692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 3315 3486 5777 844 Size: 1487 984 171 2291 3489 Fragments arranged by size: 3489 2291 1487 984 171 BsrBI CCG'CTC Cuts at: 1394 1823 2581 3241 6309 1394 Size: 429 758 660 3068 3507 Fragments arranged by size: 3507 3068 758 660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4216 4600 5193 6917 7091 65 Size: 1183 2968 384 593 1724 174 1396 Fragments arranged by size: 2968 1724 1396 1183 593 384 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2056 2725 3278 4022 4544 4727 Size: 110 223 23 669 553 744 522 183 Cuts at: 4727 4917 5562 7066 7253 1700 Size: 190 645 1504 187 2869 Fragments arranged by size: 2869 1504 744 669 645 553 522 223 190 187 183 110 23 BsrGI T'GTAC_A Cuts at: 96 3969 96 Size: 3873 4549 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8422 BssSI C'ACGA_G Cuts at: 2255 6482 7494 2255 Size: 4227 1012 3183 Fragments arranged by size: 4227 3183 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 4009 5077 794 Size: 1652 1563 1068 4139 Fragments arranged by size: 4139 1652 1563 1068 BstBI TT'CG_AA Cuts at: 7226 7226 Size: 8422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8422 BstXI CCAn_nnnn'nTGG Cuts at: 4211 4819 4211 Size: 608 7814 BstYI r'GATC_y Cuts at: 1972 3397 5074 5752 6119 6504 6521 7973 8059 Size: 1425 1677 678 367 385 17 1452 86 Cuts at: 8059 8071 8417 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1677 1452 1425 678 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5570 5570 Size: 8422 BtgI C'CryG_G Cuts at: 513 1065 2298 2562 3576 4168 4204 4632 5586 Size: 552 1233 264 1014 592 36 428 954 Cuts at: 5586 513 Size: 3349 Fragments arranged by size: 3349 1233 1014 954 592 552 428 264 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7683 Fragments arranged by size: 7683 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3610 4143 4624 4763 5938 6732 6752 3064 Size: 546 533 481 139 1175 794 20 4734 Fragments arranged by size: 4734 1175 794 546 533 481 139 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4673 4708 529 Size: 35 4109 35 4243 Fragments arranged by size: 4243 4109 35 35 DraI TTT'AAA Cuts at: 5726 5726 Size: 8422 DraIII CAC_nnn'GTG Cuts at: 3268 3268 Size: 8422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2603 7429 809 Size: 507 1287 4826 1802 Fragments arranged by size: 4826 1802 1287 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2463 2565 Size: 54 1425 50 275 18 201 432 102 Cuts at: 2565 2643 2901 3276 3402 3897 4858 5346 5869 Size: 78 258 375 126 495 961 488 523 Cuts at: 5869 6758 8387 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 961 889 523 495 488 432 375 275 258 201 126 102 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4858 5869 1537 Size: 275 3046 1011 4090 Fragments arranged by size: 4090 3046 1011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 3010 3248 4772 5268 5361 6352 1353 Size: 1335 322 238 1524 496 93 991 3423 Fragments arranged by size: 3423 1524 1335 991 496 322 238 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2411 2534 4046 7009 7383 7529 Size: 1064 138 822 123 1512 2963 374 146 Cuts at: 7529 387 Size: 1280 Fragments arranged by size: 2963 1512 1280 1064 822 374 146 138 123 EcoICRI GAG'CTC Cuts at: 2831 3367 3676 5313 2831 Size: 536 309 1637 5940 Fragments arranged by size: 5940 1637 536 309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2068 2477 2765 3308 3353 3488 Size: 393 409 27 409 288 543 45 135 Cuts at: 3488 3680 3944 4426 5273 6485 7082 7869 8137 Size: 192 264 482 847 1212 597 787 268 Cuts at: 8137 1239 Size: 1524 Fragments arranged by size: 1524 1212 847 787 597 543 482 409 409 393 288 268 264 192 135 45 27 EcoNI CCTnn'n_nnAGG Cuts at: 2855 3341 4031 5573 2855 Size: 486 690 1542 5704 Fragments arranged by size: 5704 1542 690 486 EcoO109I rG'GnC_Cy Cuts at: 2584 2749 3101 3175 3463 3475 3561 3562 3767 Size: 165 352 74 288 12 86 1 205 Cuts at: 3767 4368 4642 4975 2584 Size: 601 274 333 6031 Fragments arranged by size: 6031 601 352 333 288 274 205 165 86 74 12 1 EcoRI G'AATT_C Cuts at: 5731 6109 5731 Size: 378 8044 FseI GG_CCGG'CC Cuts at: 4026 4026 Size: 8422 FspI TGC'GCA Cuts at: 5081 6928 5081 Size: 1847 6575 HaeII r_GCGC'y Cuts at: 1678 2040 2077 4897 5389 5657 7569 1678 Size: 362 37 2820 492 268 1912 2531 Fragments arranged by size: 2820 2531 1912 492 362 268 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2820 2852 4664 4696 7063 Size: 32 135 32 1248 32 1812 32 2367 Cuts at: 7063 7095 7137 7169 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2367 1812 1248 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3064 3316 4148 5323 5766 6012 Size: 988 281 1126 252 832 1175 443 246 Cuts at: 6012 669 Size: 3079 Fragments arranged by size: 3079 1175 1126 988 832 443 281 252 246 HindIII A'AGCT_T Cuts at: 748 5183 748 Size: 4435 3987 HpaI GTT'AAC Cuts at: 6012 6012 Size: 8422 KasI G'GCGC_C Cuts at: 2036 2036 Size: 8422 KpnI G_GTAC'C Cuts at: 3724 3918 5747 3724 Size: 194 1829 6399 Fragments arranged by size: 6399 1829 194 MfeI C'AATT_G Cuts at: 6021 6021 Size: 8422 MluI A'CGCG_T Cuts at: 8298 8298 Size: 8422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 4977 5631 7536 7720 1305 Size: 822 2850 654 1905 184 2007 Fragments arranged by size: 2850 2007 1905 822 654 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 2645 2903 3404 3899 Size: 54 1425 343 735 78 258 501 495 Cuts at: 3899 5348 10 Size: 1449 3084 Fragments arranged by size: 3084 1449 1425 735 501 495 343 258 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2891 2897 4209 4269 4661 4817 Size: 648 1068 657 6 1312 60 392 156 Cuts at: 4817 5342 6380 6739 6898 518 Size: 525 1038 359 159 2042 Fragments arranged by size: 2042 1312 1068 1038 657 648 525 392 359 159 156 60 6 MspA1I CmG'CkG Cuts at: 1606 2122 2209 2300 2488 2683 2719 3373 3421 Size: 516 87 91 188 195 36 654 48 Cuts at: 3421 3932 4006 4703 4940 4983 5838 6515 7663 Size: 511 74 697 237 43 855 677 1148 Cuts at: 7663 7908 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 855 697 677 654 516 511 245 237 195 188 91 87 74 48 43 36 NaeI GCC'GGC Cuts at: 2035 2727 3280 4024 4546 4729 2035 Size: 692 553 744 522 183 5728 Fragments arranged by size: 5728 744 692 553 522 183 NarI GG'CG_CC Cuts at: 2037 2037 Size: 8422 NcoI C'CATG_G Cuts at: 513 1065 3576 4204 4632 5586 513 Size: 552 2511 628 428 954 3349 Fragments arranged by size: 3349 2511 954 628 552 428 NdeI CA'TA_TG Cuts at: 387 4064 387 Size: 3677 4745 NgoMIV G'CCGG_C Cuts at: 2033 2725 3278 4022 4544 4727 2033 Size: 692 553 744 522 183 5728 Fragments arranged by size: 5728 744 692 553 522 183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8422 NotI GC'GGCC_GC Cuts at: 5869 5869 Size: 8422 NsiI A_TGCA'T Cuts at: 4737 4737 Size: 8422 NspI r_CATG'y Cuts at: 1132 1229 2872 2896 3286 4735 4841 5494 5780 Size: 97 1643 24 390 1449 106 653 286 Cuts at: 5780 7325 1132 Size: 1545 2229 Fragments arranged by size: 2229 1643 1545 1449 653 390 286 106 97 24 PasI CC'CwG_GG Cuts at: 1348 5644 1348 Size: 4296 4126 PciI A'CATG_T Cuts at: 7321 7321 Size: 8422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2686 4441 5355 1108 Size: 1578 1755 914 4175 Fragments arranged by size: 4175 1755 1578 914 PfoI T'CCnGG_A Cuts at: 2877 3171 3703 5268 2877 Size: 294 532 1565 6031 Fragments arranged by size: 6031 1565 532 294 PmeI GTTT'AAAC Cuts at: 5726 5726 Size: 8422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6491 6523 8004 8036 6491 Size: 32 1481 32 6877 Fragments arranged by size: 6877 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 3175 3463 3475 2749 Size: 426 288 12 7696 Fragments arranged by size: 7696 426 288 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8422 PsiI TTA'TAA Cuts at: 5992 5992 Size: 8422 PspOMI G'GGCC_C Cuts at: 2583 3561 3766 4367 2583 Size: 978 205 601 6638 Fragments arranged by size: 6638 978 601 205 PstI C_TGCA'G Cuts at: 830 2328 3605 3789 4138 4344 4770 5073 5774 Size: 1498 1277 184 349 206 426 303 701 Cuts at: 5774 830 Size: 3478 Fragments arranged by size: 3478 1498 1277 701 426 349 303 206 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8422 PvuII CAG'CTG Cuts at: 2209 2488 2719 3421 3932 4006 4703 4940 2209 Size: 279 231 702 511 74 697 237 5691 Fragments arranged by size: 5691 702 697 511 279 237 231 74 SacI G_AGCT'C Cuts at: 2833 3369 3678 5315 2833 Size: 536 309 1637 5940 Fragments arranged by size: 5940 1637 536 309 SacII CC_GC'GG Cuts at: 2301 2301 Size: 8422 SalI G'TCGA_C Cuts at: 1936 5764 1936 Size: 3828 4594 SapI GCTCTTCn'nnn_ Cuts at: 2688 3248 4772 2688 Size: 560 1524 6338 Fragments arranged by size: 6338 1524 560 SbfI CC_TGCA'GG Cuts at: 4344 5073 5774 4344 Size: 729 701 6992 Fragments arranged by size: 6992 729 701 ScaI AGT'ACT Cuts at: 3188 6670 8382 3188 Size: 3482 1712 3228 Fragments arranged by size: 3482 3228 1712 SexAI A'CCwGG_T Cuts at: 5421 5483 5421 Size: 62 8360 SfcI C'TryA_G Cuts at: 826 1045 2324 3601 3785 3949 4134 4340 4766 Size: 219 1279 1277 184 164 185 206 426 Cuts at: 4766 5069 5770 6905 7586 7777 826 Size: 303 701 1135 681 191 1471 Fragments arranged by size: 1471 1279 1277 1135 701 681 426 303 219 206 191 185 184 164 SfoI GGC'GCC Cuts at: 2038 2038 Size: 8422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8422 SmaI CCC'GGG Cuts at: 1872 3765 5749 1872 Size: 1893 1984 4545 Fragments arranged by size: 4545 1984 1893 SmlI C'TyrA_G Cuts at: 820 1017 1943 2242 3123 5186 6526 7427 7689 Size: 197 926 299 881 2063 1340 901 262 Cuts at: 7689 820 Size: 1553 Fragments arranged by size: 2063 1553 1340 926 901 881 299 262 197 SnaBI TAC'GTA Cuts at: 493 6213 493 Size: 5720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8422 SphI G_CATG'C Cuts at: 1132 2872 2896 3286 4735 4841 5780 1132 Size: 1740 24 390 1449 106 939 3774 Fragments arranged by size: 3774 1740 1449 939 390 106 24 SspI AAT'ATT Cuts at: 5 52 6346 5 Size: 47 6294 2081 Fragments arranged by size: 6294 2081 47 StuI AGG'CCT Cuts at: 1517 4509 1517 Size: 2992 5430 StyI C'CwwG_G Cuts at: 513 1065 3576 4179 4204 4305 4632 4971 5586 Size: 552 2511 603 25 101 327 339 615 Cuts at: 5586 5694 5861 513 Size: 108 167 3074 Fragments arranged by size: 3074 2511 615 603 552 339 327 167 108 101 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2789 2841 3227 3279 3284 4202 4623 Size: 1255 1209 52 386 52 5 918 421 Cuts at: 4623 4655 4743 5290 6449 6466 6619 6804 7249 Size: 32 88 547 1159 17 153 185 445 Cuts at: 7249 325 Size: 1498 Fragments arranged by size: 1498 1255 1209 1159 918 547 445 421 386 185 153 88 52 52 32 17 5 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3186 3969 6668 Size: 274 80 33 51 1443 1209 783 2699 Cuts at: 6668 8380 96 Size: 1712 138 Fragments arranged by size: 2699 1712 1443 1209 783 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 3455 3487 5038 5070 8382 8414 2801 Size: 32 622 32 1551 32 3312 32 2809 Fragments arranged by size: 3312 2809 1551 622 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8422 XbaI T'CTAG_A Cuts at: 5758 5758 Size: 8422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 3701 5692 2638 Size: 1063 1991 5368 Fragments arranged by size: 5368 1991 1063 XhoI C'TCGA_G Cuts at: 1943 2242 3123 1943 Size: 299 881 7242 Fragments arranged by size: 7242 881 299 XmaI C'CCGG_G Cuts at: 1870 3763 5747 1870 Size: 1893 1984 4545 Fragments arranged by size: 4545 1984 1893 XmnI GAAnn'nnTTC Cuts at: 6551 6551 Size: 8422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6230 276 Size: 53 83 186 936 4696 2468 Fragments arranged by size: 4696 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV FalI FspAI NruI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI