(Linear) (Six-base) MAPSORT of: KIEE1087.seq Check: 2558 from: 1 to: 3717 KIEE1087.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 326 3717 Size: 326 3391 Acc65I G'GTAC_C Cuts at: 0 1715 1909 3717 Size: 1715 194 1808 Fragments arranged by size: 1808 1715 194 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1675 3717 Size: 1675 2042 AfeI AGC'GCT Cuts at: 0 2890 3650 3717 Size: 2890 760 67 Fragments arranged by size: 2890 760 67 AflIII A'CryG_T Cuts at: 0 1196 3717 Size: 1196 2521 AgeI A'CCGG_T Cuts at: 0 3557 3717 Size: 3557 160 AleI CACnn'nnGTG Cuts at: 0 2656 2812 3717 Size: 2656 156 905 Fragments arranged by size: 2656 905 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1202 2897 3717 Size: 1202 1695 820 Fragments arranged by size: 1695 1202 820 AlwNI CAG_nnn'CTG Cuts at: 0 441 681 846 1173 1653 2001 3072 3717 Size: 441 240 165 327 480 348 1071 645 Fragments arranged by size: 1071 645 480 441 348 327 240 165 ApaI G_GGCC'C Cuts at: 0 582 1560 1765 2366 3717 Size: 582 978 205 601 1351 Fragments arranged by size: 1351 978 601 582 205 ApaLI G'TGCA_C Cuts at: 0 1075 3717 Size: 1075 2642 ApoI r'AATT_y Cuts at: 0 1480 3717 Size: 1480 2237 AvaI C'yCGr_G Cuts at: 0 237 538 568 1118 1758 3717 Size: 237 301 30 550 640 1959 Fragments arranged by size: 1959 640 550 301 237 30 BanI G'GyrC_C Cuts at: 0 31 754 1138 1715 1869 1909 2329 2353 Size: 31 723 384 577 154 40 420 24 Cuts at: 2353 2480 2542 3560 3717 Size: 127 62 1018 157 Fragments arranged by size: 1018 723 577 420 384 157 154 127 62 40 31 24 BanII G_rGCy'C Cuts at: 0 335 582 828 1364 1560 1673 1765 2366 Size: 335 247 246 536 196 113 92 601 Cuts at: 2366 2570 3246 3254 3310 3717 Size: 204 676 8 56 407 Fragments arranged by size: 676 601 536 407 335 247 246 204 196 113 92 56 8 BbeI G_GCGC'C Cuts at: 0 35 3717 Size: 35 3682 BbsI GAAGACnn'nnnn_ Cuts at: 0 1448 1589 3717 Size: 1448 141 2128 Fragments arranged by size: 2128 1448 141 BbvCI CC'TCA_GC Cuts at: 0 167 1774 2102 3717 Size: 167 1607 328 1615 Fragments arranged by size: 1615 1607 328 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 228 262 3717 Size: 228 34 3455 Fragments arranged by size: 3455 228 34 BclI T'GATC_A Cuts at: 0 2474 3717 Size: 2474 1243 BfrBI ATG'CAT Cuts at: 0 2730 3717 Size: 2730 987 BglI GCCn_nnn'nGGC Cuts at: 0 1683 1878 2023 2574 3717 Size: 1683 195 145 551 1143 Fragments arranged by size: 1683 1143 551 195 145 BlpI GC'TnA_GC Cuts at: 0 910 2885 3717 Size: 910 1975 832 Fragments arranged by size: 1975 910 832 Bme1580I G_kGCm'C Cuts at: 0 356 582 806 1079 1141 1191 1351 1560 Size: 356 226 224 273 62 50 160 209 Cuts at: 1560 1765 2366 2785 3157 3717 Size: 205 601 419 372 560 Fragments arranged by size: 601 560 419 372 356 273 226 224 209 205 160 62 50 BmgBI CAC'GTC Cuts at: 0 366 594 1197 2223 3717 Size: 366 228 603 1026 1494 Fragments arranged by size: 1494 1026 603 366 228 BmrI ACTGGGnnnn_n' Cuts at: 0 1255 1329 3025 3516 3620 3717 Size: 1255 74 1696 491 104 97 Fragments arranged by size: 1696 1255 491 104 97 74 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 714 3717 Size: 714 3003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 63 472 760 1303 1348 1483 1939 Size: 36 27 409 288 543 45 135 456 Cuts at: 1939 2421 3268 3717 Size: 482 847 449 Fragments arranged by size: 847 543 482 456 449 409 288 135 45 36 27 Bpu10I CC'TnA_GC Cuts at: 0 167 1774 2102 3717 Size: 167 1607 328 1615 Fragments arranged by size: 1615 1607 328 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3202 3717 Size: 3202 515 BsaI GGTCTCn'nnnn_ Cuts at: 0 81 3717 Size: 81 3636 BsaAI yAC'GTr Cuts at: 0 3211 3717 Size: 3211 506 BsaHI Gr'CG_yC Cuts at: 0 32 2711 3717 Size: 32 2679 1006 Fragments arranged by size: 2679 1006 32 BsaWI w'CCGG_w Cuts at: 0 1718 3557 3717 Size: 1718 1839 160 Fragments arranged by size: 1839 1718 160 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1597 1627 3717 Size: 1597 30 2090 Fragments arranged by size: 2090 1597 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 586 1372 1618 1621 1624 1762 2044 3442 Size: 586 786 246 3 3 138 282 1398 Cuts at: 3442 3717 Size: 275 Fragments arranged by size: 1398 786 586 282 275 246 138 3 3 BseYI C'CCAG_C Cuts at: 0 71 103 479 1017 1552 1688 1765 1856 Size: 71 32 376 538 535 136 77 91 Cuts at: 1856 1927 2183 2188 2869 2945 3222 3501 3717 Size: 71 256 5 681 76 277 279 216 Fragments arranged by size: 681 538 535 376 279 277 256 216 136 91 77 76 71 71 32 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 690 1819 2653 2721 3563 3717 Size: 232 458 1129 834 68 842 154 Fragments arranged by size: 1129 842 834 458 232 154 68 BsiEI CG_ry'CG Cuts at: 0 2856 3717 Size: 2856 861 BsiHKAI G_wGCw'C Cuts at: 0 317 828 1079 1364 1673 2373 3310 3717 Size: 317 511 251 285 309 700 937 407 Fragments arranged by size: 937 700 511 407 317 309 285 251 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2702 3717 Size: 2702 1015 Bsp1286I G_dGCh'C Cuts at: 0 317 335 356 582 806 828 1079 1141 Size: 317 18 21 226 224 22 251 62 Cuts at: 1141 1191 1351 1364 1560 1673 1765 2366 2373 Size: 50 160 13 196 113 92 601 7 Cuts at: 2373 2570 2785 3157 3246 3254 3310 3717 Size: 197 215 372 89 8 56 407 Fragments arranged by size: 601 407 372 317 251 226 224 215 197 196 160 113 92 89 62 56 50 22 21 18 13 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 326 1310 1481 3717 Size: 326 984 171 2236 Fragments arranged by size: 2236 984 326 171 BsrBI CCG'CTC Cuts at: 0 576 1236 3717 Size: 576 660 2481 Fragments arranged by size: 2481 660 576 BsrDI GCAATG_nn' Cuts at: 0 2211 2595 3188 3717 Size: 2211 384 593 529 Fragments arranged by size: 2211 593 529 384 BsrFI r'CCGG_y Cuts at: 0 28 51 720 1273 2017 2539 2722 2912 Size: 28 23 669 553 744 522 183 190 Cuts at: 2912 3557 3717 Size: 645 160 Fragments arranged by size: 744 669 645 553 522 190 183 160 28 23 BsrGI T'GTAC_A Cuts at: 0 1964 3717 Size: 1964 1753 BssSI C'ACGA_G Cuts at: 0 250 3717 Size: 250 3467 BstAPI GCAn_nnn'nTGC Cuts at: 0 441 2004 3072 3717 Size: 441 1563 1068 645 Fragments arranged by size: 1563 1068 645 441 BstXI CCAn_nnnn'nTGG Cuts at: 0 2206 2814 3717 Size: 2206 608 903 Fragments arranged by size: 2206 903 608 BstYI r'GATC_y Cuts at: 0 1392 3069 3717 Size: 1392 1677 648 Fragments arranged by size: 1677 1392 648 Bsu36I CC'TnA_GG Cuts at: 0 3565 3717 Size: 3565 152 BtgI C'CryG_G Cuts at: 0 293 557 1571 2163 2199 2627 3581 3717 Size: 293 264 1014 592 36 428 954 136 Fragments arranged by size: 1014 954 592 428 293 264 136 36 BtsI GCAGTG_nn' Cuts at: 0 1059 1605 2138 2619 2758 3717 Size: 1059 546 533 481 139 959 Fragments arranged by size: 1059 959 546 533 481 139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2668 2703 3717 Size: 2668 35 1014 Fragments arranged by size: 2668 1014 35 DraIII CAC_nnn'GTG Cuts at: 0 1263 3717 Size: 1263 2454 DrdI GACnn_nn'nnGTC Cuts at: 0 598 3717 Size: 598 3119 EaeI y'GGCC_r Cuts at: 0 26 458 560 638 896 1271 1397 1892 Size: 26 432 102 78 258 375 126 495 Cuts at: 1892 2853 3341 3717 Size: 961 488 376 Fragments arranged by size: 961 495 488 432 376 375 258 126 102 78 26 EagI C'GGCC_G Cuts at: 0 2853 3717 Size: 2853 864 EarI CTCTTCn'nnn_ Cuts at: 0 683 1005 1243 2767 3263 3356 3717 Size: 683 322 238 1524 496 93 361 Fragments arranged by size: 1524 683 496 361 322 238 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 406 529 2041 3717 Size: 406 123 1512 1676 Fragments arranged by size: 1676 1512 406 123 EcoICRI GAG'CTC Cuts at: 0 826 1362 1671 3308 3717 Size: 826 536 309 1637 409 Fragments arranged by size: 1637 826 536 409 309 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 63 472 760 1303 1348 1483 1675 Size: 36 27 409 288 543 45 135 192 Cuts at: 1675 1939 2421 3268 3717 Size: 264 482 847 449 Fragments arranged by size: 847 543 482 449 409 288 264 192 135 45 36 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 850 1336 2026 3568 3717 Size: 850 486 690 1542 149 Fragments arranged by size: 1542 850 690 486 149 EcoO109I rG'GnC_Cy Cuts at: 0 579 744 1096 1170 1458 1470 1556 1557 Size: 579 165 352 74 288 12 86 1 Cuts at: 1557 1762 2363 2637 2970 3717 Size: 205 601 274 333 747 Fragments arranged by size: 747 601 579 352 333 288 274 205 165 86 74 12 1 FseI GG_CCGG'CC Cuts at: 0 2021 3717 Size: 2021 1696 FspI TGC'GCA Cuts at: 0 3076 3717 Size: 3076 641 HaeII r_GCGC'y Cuts at: 0 35 72 2892 3384 3652 3717 Size: 35 37 2820 492 268 65 Fragments arranged by size: 2820 492 268 65 37 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 815 847 2659 2691 3717 Size: 815 32 1812 32 1026 Fragments arranged by size: 1812 1026 815 32 32 HincII GTy'rAC Cuts at: 0 1059 1311 2143 3318 3717 Size: 1059 252 832 1175 399 Fragments arranged by size: 1175 1059 832 399 252 HindIII A'AGCT_T Cuts at: 0 3178 3717 Size: 3178 539 KasI G'GCGC_C Cuts at: 0 31 3717 Size: 31 3686 KpnI G_GTAC'C Cuts at: 0 1719 1913 3717 Size: 1719 194 1804 Fragments arranged by size: 1804 1719 194 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 122 2972 3626 3717 Size: 122 2850 654 91 Fragments arranged by size: 2850 654 122 91 MscI TGG'CCA Cuts at: 0 562 640 898 1399 1894 3343 3717 Size: 562 78 258 501 495 1449 374 Fragments arranged by size: 1449 562 501 495 374 258 78 MslI CAynn'nnrTG Cuts at: 0 229 886 892 2204 2264 2656 2812 3337 Size: 229 657 6 1312 60 392 156 525 Cuts at: 3337 3717 Size: 380 Fragments arranged by size: 1312 657 525 392 380 229 156 60 6 MspA1I CmG'CkG Cuts at: 0 117 204 295 483 678 714 1368 1416 Size: 117 87 91 188 195 36 654 48 Cuts at: 1416 1927 2001 2698 2935 2978 3717 Size: 511 74 697 237 43 739 Fragments arranged by size: 739 697 654 511 237 195 188 117 91 87 74 48 43 36 NaeI GCC'GGC Cuts at: 0 30 722 1275 2019 2541 2724 3717 Size: 30 692 553 744 522 183 993 Fragments arranged by size: 993 744 692 553 522 183 30 NarI GG'CG_CC Cuts at: 0 32 3717 Size: 32 3685 NcoI C'CATG_G Cuts at: 0 1571 2199 2627 3581 3717 Size: 1571 628 428 954 136 Fragments arranged by size: 1571 954 628 428 136 NdeI CA'TA_TG Cuts at: 0 2059 3717 Size: 2059 1658 NgoMIV G'CCGG_C Cuts at: 0 28 720 1273 2017 2539 2722 3717 Size: 28 692 553 744 522 183 995 Fragments arranged by size: 995 744 692 553 522 183 28 NsiI A_TGCA'T Cuts at: 0 2732 3717 Size: 2732 985 NspI r_CATG'y Cuts at: 0 867 891 1281 2730 2836 3489 3717 Size: 867 24 390 1449 106 653 228 Fragments arranged by size: 1449 867 653 390 228 106 24 PasI CC'CwG_GG Cuts at: 0 3639 3717 Size: 3639 78 PflMI CCAn_nnn'nTGG Cuts at: 0 681 2436 3350 3717 Size: 681 1755 914 367 Fragments arranged by size: 1755 914 681 367 PfoI T'CCnGG_A Cuts at: 0 872 1166 1698 3263 3717 Size: 872 294 532 1565 454 Fragments arranged by size: 1565 872 532 454 294 PpuMI rG'GwC_Cy Cuts at: 0 744 1170 1458 1470 3717 Size: 744 426 288 12 2247 Fragments arranged by size: 2247 744 426 288 12 PspOMI G'GGCC_C Cuts at: 0 578 1556 1761 2362 3717 Size: 578 978 205 601 1355 Fragments arranged by size: 1355 978 601 578 205 PstI C_TGCA'G Cuts at: 0 323 1600 1784 2133 2339 2765 3068 3717 Size: 323 1277 184 349 206 426 303 649 Fragments arranged by size: 1277 649 426 349 323 303 206 184 PvuII CAG'CTG Cuts at: 0 204 483 714 1416 1927 2001 2698 2935 Size: 204 279 231 702 511 74 697 237 Cuts at: 2935 3717 Size: 782 Fragments arranged by size: 782 702 697 511 279 237 231 204 74 SacI G_AGCT'C Cuts at: 0 828 1364 1673 3310 3717 Size: 828 536 309 1637 407 Fragments arranged by size: 1637 828 536 407 309 SacII CC_GC'GG Cuts at: 0 296 3717 Size: 296 3421 SapI GCTCTTCn'nnn_ Cuts at: 0 683 1243 2767 3717 Size: 683 560 1524 950 Fragments arranged by size: 1524 950 683 560 SbfI CC_TGCA'GG Cuts at: 0 2339 3068 3717 Size: 2339 729 649 Fragments arranged by size: 2339 729 649 ScaI AGT'ACT Cuts at: 0 1183 3717 Size: 1183 2534 SexAI A'CCwGG_T Cuts at: 0 3416 3478 3717 Size: 3416 62 239 Fragments arranged by size: 3416 239 62 SfcI C'TryA_G Cuts at: 0 319 1596 1780 1944 2129 2335 2761 3064 Size: 319 1277 184 164 185 206 426 303 Cuts at: 3064 3717 Size: 653 Fragments arranged by size: 1277 653 426 319 303 206 185 184 164 SfoI GGC'GCC Cuts at: 0 33 3717 Size: 33 3684 SmaI CCC'GGG Cuts at: 0 1760 3717 Size: 1760 1957 SmlI C'TyrA_G Cuts at: 0 237 1118 3181 3717 Size: 237 881 2063 536 Fragments arranged by size: 2063 881 536 237 SphI G_CATG'C Cuts at: 0 867 891 1281 2730 2836 3717 Size: 867 24 390 1449 106 881 Fragments arranged by size: 1449 881 867 390 106 24 StuI AGG'CCT Cuts at: 0 2504 3717 Size: 2504 1213 StyI C'CwwG_G Cuts at: 0 1571 2174 2199 2300 2627 2966 3581 3689 Size: 1571 603 25 101 327 339 615 108 Cuts at: 3689 3717 Size: 28 Fragments arranged by size: 1571 615 603 339 327 108 101 28 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 784 836 1222 1274 1279 2197 2618 2650 Size: 784 52 386 52 5 918 421 32 Cuts at: 2650 2738 3285 3717 Size: 88 547 432 Fragments arranged by size: 918 784 547 432 421 386 88 52 52 32 5 TatI w'GTAC_w Cuts at: 0 1181 1964 3717 Size: 1181 783 1753 Fragments arranged by size: 1753 1181 783 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 796 828 1450 1482 3033 3065 3717 Size: 796 32 622 32 1551 32 652 Fragments arranged by size: 1551 796 652 622 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 633 1696 3687 3717 Size: 633 1063 1991 30 Fragments arranged by size: 1991 1063 633 30 XhoI C'TCGA_G Cuts at: 0 237 1118 3717 Size: 237 881 2599 Fragments arranged by size: 2599 881 237 XmaI C'CCGG_G Cuts at: 0 1758 3717 Size: 1758 1959 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AflII AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI BglII BmtI BsaBI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI EcoRI EcoRV FalI FspAI HpaI MfeI MluI NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmnI ZraI