Restriction map for
pFN21AB6131
(Circular) (Six-base) MAPSORT of: pFN21AB6131.seq Check: 6799 from: 1 to: 6406
pFN21AB6131 6406 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2560 2884 3384 3408 2560
Size: 324 500 24 5558
Fragments arranged by size:
5558 500 324 24
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2658 3602 4469 5853 6121 1632
Size: 893 133 944 867 1384 268 1917
Fragments arranged by size:
1917 1384 944 893 867 268 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2779 3162 5305 6282 1203
Size: 1576 383 2143 977 1327
Fragments arranged by size:
2143 1576 1327 977 383
AgeI A'CCGG_T
Cuts at: 3333 5237 3333
Size: 1904 4502
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2831 1184
Size: 1647 4759
AlwNI CAG_nnn'CTG
Cuts at: 2188 2215 2473 3694 4149 5721 2188
Size: 27 258 1221 455 1572 2873
Fragments arranged by size:
2873 1572 1221 455 258 27
ApaI G_GGCC'C
Cuts at: 2111 2111
Size: 6406
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 3715 3941 4093 6202 1493
Size: 2222 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2112 2229 2315 2593 3731 1870
Size: 73 114 55 117 86 278 1138 4545
Fragments arranged by size:
4545 1138 278 117 114 86 73 55
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 2929 3300 3341 3561
Size: 325 217 627 1109 33 371 41 220
Cuts at: 3561 3727 5182 618
Size: 166 1455 1842
Fragments arranged by size:
1842 1455 1109 627 371 325 220 217
166 41 33
BanII G_rGCy'C
Cuts at: 1391 2015 2111 2316 2418 2481 3077 3725 1391
Size: 624 96 205 102 63 596 648 4072
Fragments arranged by size:
4072 648 624 596 205 102 96 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 928
Size: 971 456 4979
Fragments arranged by size:
4979 971 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2720 2754 4597 4631 1903
Size: 34 783 34 1843 34 3678
Fragments arranged by size:
3678 1843 783 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3387 4298 5508 3387
Size: 911 1210 4285
Fragments arranged by size:
4285 1210 911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2167 5017 136
Size: 107 122 71 631 605 495 2850 1525
Fragments arranged by size:
2850 1525 631 605 495 122 107 71
BglII A'GATC_T
Cuts at: 2749 6401 2749
Size: 3652 2754
BlpI GC'TnA_GC
Cuts at: 2504 3823 2504
Size: 1319 5087
Bme1580I G_kGCm'C
Cuts at: 1790 2111 2820 3303 4467 5623 1790
Size: 321 709 483 1164 1156 2573
Fragments arranged by size:
2573 1164 1156 709 483 321
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2510 4137 5095
Size: 416 53 83 399 24 1093 1627 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1753 1627 1093 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2210 2225 2567 2786 2834 3001 3214
Size: 801 170 15 342 219 48 167 213
Cuts at: 3214 3401 5066 1239
Size: 187 1665 2579
Fragments arranged by size:
2579 1665 801 342 219 213 187 170
167 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 4531 5396 5694 2321
Size: 2210 865 298 3033
Fragments arranged by size:
3033 2210 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2314 2719 2830 5069 882
Size: 617 815 405 111 2239 2219
Fragments arranged by size:
2239 2219 815 617 405 111
BsaAI yAC'GTr
Cuts at: 493 1204 3163 4197 493
Size: 711 1959 1034 2702
Fragments arranged by size:
2702 1959 1034 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 4213 4595 5230
Size: 53 83 186 936 538 2142 382 635
Cuts at: 5230 275
Size: 1451
Fragments arranged by size:
2142 1451 936 635 538 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3111 3333 4839 5237 5511 5658 6069 3111
Size: 222 1506 398 274 147 411 3448
Fragments arranged by size:
3448 1506 411 398 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3020 3050 3123 3153 3365
Size: 30 165 30 1617 30 73 30 212
Cuts at: 3365 3395 1178
Size: 30 4189
Fragments arranged by size:
4189 1617 212 165 73 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2780 2900 3638 1186
Size: 837 757 120 738 3954
Fragments arranged by size:
3954 837 757 738 120
BseYI C'CCAG_C
Cuts at: 2051 2244 2448 2742 2947 3039 3067 5609 6105
Size: 193 204 294 205 92 28 2542 496
Cuts at: 6105 2051
Size: 2352
Fragments arranged by size:
2542 2352 496 294 205 204 193 92
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3040 3161 3650 1163
Size: 573 1304 121 489 3919
Fragments arranged by size:
3919 1304 573 489 121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3257 4467 4552 5623 3257
Size: 1210 85 1071 4040
Fragments arranged by size:
4040 1210 1071 85
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
BsmBI CGTCTCn'nnnn_
Cuts at: 2078 2078
Size: 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2111 2316 2418 2481 2820 3077
Size: 399 225 96 205 102 63 339 257
Cuts at: 3077 3257 3303 3725 4467 4552 5623 1391
Size: 180 46 422 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 422 399 339 257 225
205 180 102 96 85 63 46
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 2884 3384 3408 3761 844
Size: 1716 324 500 24 353 3489
Fragments arranged by size:
3489 1716 500 353 324 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2271 4293 1394
Size: 429 448 2022 3507
Fragments arranged by size:
3507 2022 448 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2407 3333 3387 3421 3609 5050 5237
Size: 110 597 926 54 34 188 1441 187
Cuts at: 5237 1700
Size: 2869
Fragments arranged by size:
2869 1441 926 597 188 187 110 54
34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 3248 3314 3656 4466 5478 3248
Size: 66 342 810 1012 4176
Fragments arranged by size:
4176 1012 810 342 66
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 2572 1184
Size: 1388 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2461
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 2235 2659 2749 2797 3736 4103 4488 4505
Size: 263 424 90 48 939 367 385 17
Cuts at: 4505 5957 6043 6055 6401 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 939 424 385 367 346 263
90 86 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2289 513
Size: 552 1224 4630
Fragments arranged by size:
4630 1224 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2336 3922 4716 4736 2336
Size: 1586 794 20 4006
Fragments arranged by size:
4006 1586 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2286 3853
Size: 54 1425 50 275 18 387 69 1567
Cuts at: 3853 4742 6371 8
Size: 889 1629 43
Fragments arranged by size:
1629 1567 1425 889 387 275 69 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2541 2634 3057 3347 3425 4336 1353
Size: 728 460 93 423 290 78 911 3423
Fragments arranged by size:
3423 911 728 460 423 290 93 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 3350 3406 3641 4993 5367
Size: 1064 138 480 1281 56 235 1352 374
Cuts at: 5367 5513 387
Size: 146 1280
Fragments arranged by size:
1352 1281 1280 1064 480 374 235 146
138 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2210 2225 2525 2567 2658 2786
Size: 393 408 170 15 300 42 91 128
Cuts at: 2786 2834 3001 3214 3401 3602 4469 5066 5853
Size: 48 167 213 187 201 867 597 787
Cuts at: 5853 6121 1239
Size: 268 1524
Fragments arranged by size:
1524 867 787 597 408 393 300 268
213 201 187 170 167 128 91 48
42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2540
Size: 6406
EcoO109I rG'GnC_Cy
Cuts at: 2108 2119 2137 2512 3289 3467 2108
Size: 11 18 375 777 178 5047
Fragments arranged by size:
5047 777 375 178 18 11
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2131 3664 3679 5553 1678
Size: 453 1533 15 1874 2531
Fragments arranged by size:
2531 1874 1533 453 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 2676 2708 3020
Size: 32 135 32 1061 32 11 32 312
Cuts at: 3020 3052 5047 5079 5121 5153 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 1061 312 135 42 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3202 3750 3996 669
Size: 988 281 1264 548 246 3079
Fragments arranged by size:
3079 1264 988 548 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 2779 6282 2779
Size: 3503 2903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 10
Size: 54 1425 343 387 4197
Fragments arranged by size:
4197 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4364 4723 4882 518
Size: 648 3198 359 159 2042
Fragments arranged by size:
3198 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2291 2404 2508 2951 3192 3481 3541 3822
Size: 685 113 104 443 241 289 60 281
Cuts at: 3822 4499 5647 5892 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 685 677 443 289 281 245
241 113 104 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2038 2758 1348
Size: 690 720 4996
Fragments arranged by size:
4996 720 690
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3424 1108
Size: 2316 4090
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2512 3289 3467 2137
Size: 375 777 178 5076
Fragments arranged by size:
5076 777 375 178
PshAI GACnn'nnGTC
Cuts at: 1613 3465 1613
Size: 1852 4554
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2107 2107
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2127 2895 3405 3758 830
Size: 1297 768 510 353 3478
Fragments arranged by size:
3478 1297 768 510 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2404 2951 2404
Size: 547 5859
SacII CC_GC'GG
Cuts at: 2292 2292
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 2512 3467 2512
Size: 955 5451
SapI GCTCTTCn'nnn_
Cuts at: 3057 3347 3057
Size: 290 6116
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2123 2891 3401 3754 4889 5570 5761
Size: 219 1078 768 510 353 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1471 1135 1078 768 681 510 353 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 3387 1700
Size: 1687 4719
SmaI CCC'GGG
Cuts at: 1872 2114 2231 2317 3733 1872
Size: 242 117 86 1416 4545
Fragments arranged by size:
4545 1416 242 117 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 4510 5411 5673 820
Size: 197 926 393 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2912 3533 1517
Size: 1395 621 4390
Fragments arranged by size:
4390 1395 621
StyI C'CwwG_G
Cuts at: 513 1065 2175 3845 513
Size: 552 1110 1670 3074
Fragments arranged by size:
3074 1670 1110 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 4433 4450 4603 4788 5233 325
Size: 1255 656 2197 17 153 185 445 1498
Fragments arranged by size:
2197 1498 1255 656 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 3365 3397 6366 6398 3123
Size: 32 210 32 2969 32 3131
Fragments arranged by size:
3131 2969 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 2656 2971 3742 2656
Size: 315 771 5320
Fragments arranged by size:
5320 771 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3478 3478
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2112 2229 2315 3731 1870
Size: 242 117 86 1416 4545
Fragments arranged by size:
4545 1416 242 117 86
XmnI GAAnn'nnTTC
Cuts at: 3185 3356 3533 4535 3185
Size: 171 177 1002 5056
Fragments arranged by size:
5056 1002 177 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SexAI SfiI SfoI SrfI SwaI