(Circular) (Six-base) MAPSORT of: pFN21AB0410.seq Check: 2044 from: 1 to: 7171 pFN21AB0410 7171 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2529 2529 Size: 7171 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4514 1937 Size: 2577 4594 Acc65I G'GTAC_C Cuts at: 4492 4492 Size: 7171 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3573 3765 5234 6618 6886 1632 Size: 1941 192 1469 1384 268 1917 Fragments arranged by size: 1941 1917 1469 1384 268 192 AfeI AGC'GCT Cuts at: 2137 2137 Size: 7171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6974 AflIII A'CryG_T Cuts at: 1203 6070 7047 1203 Size: 4867 977 1327 Fragments arranged by size: 4867 1327 977 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 5900 5900 Size: 7171 AleI CACnn'nnGTG Cuts at: 1166 2327 1166 Size: 1161 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2282 2345 1184 Size: 1098 63 6010 Fragments arranged by size: 6010 1098 63 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 2438 Size: 32 7139 AlwNI CAG_nnn'CTG Cuts at: 2308 3580 4914 6486 2308 Size: 1272 1334 1572 2993 Fragments arranged by size: 2993 1572 1334 1272 ApaI G_GGCC'C Cuts at: 2037 2133 2037 Size: 96 7075 ApaLI G'TGCA_C Cuts at: 2288 3116 5228 6384 2288 Size: 828 2112 1156 3075 Fragments arranged by size: 3075 2112 1156 828 ApoI r'AATT_y Cuts at: 1493 2399 3027 4480 4706 4858 6967 1493 Size: 906 628 1453 226 152 2109 1697 Fragments arranged by size: 2109 1697 1453 906 628 226 152 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2125 2296 2702 4496 1870 Size: 73 82 100 171 406 1794 4545 Fragments arranged by size: 4545 1794 406 171 100 82 73 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 2541 4492 5947 618 Size: 325 217 627 542 212 1951 1455 1842 Fragments arranged by size: 1951 1842 1455 627 542 325 217 212 BanII G_rGCy'C Cuts at: 1391 2037 2133 2277 3455 3948 4490 1391 Size: 646 96 144 1178 493 542 4072 Fragments arranged by size: 4072 1178 646 542 493 144 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3203 4459 928 Size: 971 1304 1256 3640 Fragments arranged by size: 3640 1304 1256 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2033 2067 2960 2994 5362 5396 1903 Size: 34 96 34 893 34 2368 34 3678 Fragments arranged by size: 3678 2368 893 96 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 3495 1548 Size: 1947 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 5782 136 Size: 107 122 71 631 605 349 3761 1525 Fragments arranged by size: 3761 1525 631 605 349 122 107 71 BglII A'GATC_T Cuts at: 2634 4260 7166 2634 Size: 1626 2906 2639 Fragments arranged by size: 2906 2639 1626 BlpI GC'TnA_GC Cuts at: 3573 4588 3573 Size: 1015 6156 Bme1580I G_kGCm'C Cuts at: 1790 2037 2133 2292 2334 3120 5232 6388 1790 Size: 247 96 159 42 786 2112 1156 2573 Fragments arranged by size: 2573 2112 1156 786 247 159 96 42 BmgBI CAC'GTC Cuts at: 1318 2287 1318 Size: 969 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2741 2937 4902 Size: 416 53 83 399 24 1324 196 1965 Cuts at: 4902 5860 442 Size: 958 1753 Fragments arranged by size: 1965 1753 1324 958 416 399 196 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 3313 3993 4002 4386 5831 1239 Size: 1052 1022 680 9 384 1445 2579 Fragments arranged by size: 2579 1445 1052 1022 680 384 9 Bpu10I CC'TnA_GC Cuts at: 4236 4236 Size: 7171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5296 6161 6459 5296 Size: 865 298 6008 Fragments arranged by size: 6008 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2517 2651 5834 882 Size: 617 1018 134 3183 2219 Fragments arranged by size: 3183 2219 1018 617 134 BsaAI yAC'GTr Cuts at: 493 1204 4962 493 Size: 711 3758 2702 Fragments arranged by size: 3758 2702 711 BsaBI GATnn'nnATC Cuts at: 7165 7165 Size: 7171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4978 5360 5995 275 Size: 53 83 186 936 3445 382 635 1451 Fragments arranged by size: 3445 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4317 5604 6002 6276 6423 6834 4317 Size: 1287 398 274 147 411 4654 Fragments arranged by size: 4654 1287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6946 Fragments arranged by size: 6946 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2084 2345 2740 3934 3956 1186 Size: 895 3 261 395 1194 22 4401 Fragments arranged by size: 4401 1194 895 395 261 22 3 BseYI C'CCAG_C Cuts at: 2132 2781 6374 6870 2132 Size: 649 3593 496 2433 Fragments arranged by size: 3593 2433 649 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4079 4167 1163 Size: 573 2343 88 4167 Fragments arranged by size: 4167 2343 573 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2175 4621 5382 6410 1540 Size: 275 186 15 159 2446 761 1028 2301 Fragments arranged by size: 2446 2301 1028 761 275 186 159 15 BsiHKAI G_wGCw'C Cuts at: 2277 2292 2829 2973 3120 4242 5232 5317 6388 Size: 15 537 144 147 1122 990 85 1071 Cuts at: 6388 2277 Size: 3060 Fragments arranged by size: 3060 1122 1071 990 537 147 144 85 15 BsmI GAATG_Cn' Cuts at: 2141 2810 4682 4775 2141 Size: 669 1872 93 4537 Fragments arranged by size: 4537 1872 669 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2133 2277 2292 2334 2829 2973 Size: 399 247 96 144 15 42 495 144 Cuts at: 2973 3120 3455 3948 4242 4490 5232 5317 6388 Size: 147 335 493 294 248 742 85 1071 Cuts at: 6388 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 495 493 399 335 294 248 247 147 144 144 96 85 42 15 BspEI T'CCGG_A Cuts at: 6834 6834 Size: 7171 BspHI T'CATG_A Cuts at: 5060 6790 5060 Size: 1730 5441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 2776 3601 4526 844 Size: 1685 247 825 925 3489 Fragments arranged by size: 3489 1685 925 825 247 BsrBI CCG'CTC Cuts at: 1394 1823 2199 2341 5058 1394 Size: 429 376 142 2717 3507 Fragments arranged by size: 3507 2717 429 376 142 BsrDI GCAATG_nn' Cuts at: 65 1248 3856 4383 5666 5840 65 Size: 1183 2608 527 1283 174 1396 Fragments arranged by size: 2608 1396 1283 1183 527 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2170 5815 6002 1700 Size: 110 360 3645 187 2869 Fragments arranged by size: 3645 2869 360 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 3910 794 Size: 3116 4055 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstXI CCAn_nnnn'nTGG Cuts at: 3895 3895 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2634 4260 4408 4501 4868 5253 5270 6722 Size: 662 1626 148 93 367 385 17 1452 Cuts at: 6722 6808 6820 7166 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1626 1452 662 385 367 346 148 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2463 3746 2463 Size: 1283 5888 BtgI C'CryG_G Cuts at: 513 1065 2037 2247 2421 2517 2562 4132 513 Size: 552 972 210 174 96 45 1570 3552 Fragments arranged by size: 3552 1570 972 552 210 174 96 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 2541 487 Size: 683 56 1019 296 5117 Fragments arranged by size: 5117 1019 683 296 56 BtsI GCAGTG_nn' Cuts at: 2612 2827 3272 3453 3712 4687 5481 5501 2612 Size: 215 445 181 259 975 794 20 4282 Fragments arranged by size: 4282 975 794 445 259 215 181 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 3648 4475 3648 Size: 827 6344 DraIII CAC_nnn'GTG Cuts at: 2236 2236 Size: 7171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2423 6178 809 Size: 507 1107 3755 1802 Fragments arranged by size: 3755 1802 1107 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2172 3471 Size: 54 1425 50 275 18 183 159 1299 Cuts at: 3471 4303 4618 5507 7136 8 Size: 832 315 889 1629 43 Fragments arranged by size: 1629 1425 1299 889 832 315 275 183 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2172 4618 1537 Size: 275 201 159 2446 4090 Fragments arranged by size: 4090 2446 275 201 159 EarI CTCTTCn'nnn_ Cuts at: 1353 2911 3996 4158 5101 1353 Size: 1558 1085 162 943 3423 Fragments arranged by size: 3423 1558 1085 943 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 5758 6132 6278 387 Size: 1064 138 546 3623 374 146 1280 Fragments arranged by size: 3623 1280 1064 546 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 7171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 3313 3573 3765 3993 4002 4386 Size: 393 659 1022 260 192 228 9 384 Cuts at: 4386 5234 5831 6618 6886 1239 Size: 848 597 787 268 1524 Fragments arranged by size: 1524 1022 848 787 659 597 393 384 268 260 228 192 9 EcoO109I rG'GnC_Cy Cuts at: 2033 2129 2305 2416 2686 2033 Size: 96 176 111 270 6518 Fragments arranged by size: 6518 270 176 111 96 EcoRI G'AATT_C Cuts at: 4480 4858 4480 Size: 378 6793 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3210 4093 3210 Size: 883 6288 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 2139 6318 1678 Size: 461 4179 2531 Fragments arranged by size: 4179 2531 461 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3742 3774 5812 5844 5886 Size: 32 135 32 2170 32 2038 32 42 Cuts at: 5886 5918 1373 Size: 32 2626 Fragments arranged by size: 2626 2170 2038 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2623 3517 4515 4761 669 Size: 988 281 685 894 998 246 3079 Fragments arranged by size: 3079 998 988 894 685 281 246 HindIII A'AGCT_T Cuts at: 748 3681 3734 748 Size: 2933 53 4185 Fragments arranged by size: 4185 2933 53 HpaI GTT'AAC Cuts at: 3517 4761 3517 Size: 1244 5927 KpnI G_GTAC'C Cuts at: 4496 4496 Size: 7171 MfeI C'AATT_G Cuts at: 2919 3279 4770 2919 Size: 360 1491 5320 Fragments arranged by size: 5320 1491 360 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 6285 6469 1305 Size: 1119 3861 184 2007 Fragments arranged by size: 3861 2007 1119 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3473 4305 10 Size: 54 1425 343 1641 832 2876 Fragments arranged by size: 2876 1641 1425 832 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2327 5129 5488 5647 518 Size: 648 1098 63 2802 359 159 2042 Fragments arranged by size: 2802 2042 1098 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2039 2114 2212 2349 2508 2617 2878 3352 Size: 433 75 98 137 159 109 261 474 Cuts at: 3352 3569 4134 4587 5264 6412 6657 1606 Size: 217 565 453 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 474 453 433 261 245 217 159 137 109 98 75 NaeI GCC'GGC Cuts at: 2172 2172 Size: 7171 NcoI C'CATG_G Cuts at: 513 1065 2517 2562 513 Size: 552 1452 45 5122 Fragments arranged by size: 5122 1452 552 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 7171 NgoMIV G'CCGG_C Cuts at: 2170 2170 Size: 7171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2740 4529 6074 1132 Size: 97 1511 1789 1545 2229 Fragments arranged by size: 2229 1789 1545 1511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7171 PciI A'CATG_T Cuts at: 6070 6070 Size: 7171 PflMI CCAn_nnn'nTGG Cuts at: 1108 2308 1108 Size: 1200 5971 PfoI T'CCnGG_A Cuts at: 3579 4011 3579 Size: 432 6739 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2416 2305 Size: 111 7060 PshAI GACnn'nnGTC Cuts at: 1613 2747 1613 Size: 1134 6037 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PspOMI G'GGCC_C Cuts at: 2033 2129 2033 Size: 96 7075 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3304 3336 3304 Size: 32 7139 PstI C_TGCA'G Cuts at: 830 2187 2607 3298 3915 4523 830 Size: 1357 420 691 617 608 3478 Fragments arranged by size: 3478 1357 691 617 608 420 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 2349 2508 2617 2878 3352 3569 2349 Size: 159 109 261 474 217 5951 Fragments arranged by size: 5951 474 261 217 159 109 SacI G_AGCT'C Cuts at: 2277 2277 Size: 7171 SacII CC_GC'GG Cuts at: 2040 4135 2040 Size: 2095 5076 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SbfI CC_TGCA'GG Cuts at: 3915 4523 3915 Size: 608 6563 ScaI AGT'ACT Cuts at: 5419 7131 5419 Size: 1712 5459 SexAI A'CCwGG_T Cuts at: 2307 3528 2307 Size: 1221 5950 SfcI C'TryA_G Cuts at: 826 1045 2183 2603 3294 3911 4243 4519 5654 Size: 219 1138 420 691 617 332 276 1135 Cuts at: 5654 6335 6526 826 Size: 681 191 1471 Fragments arranged by size: 1471 1138 1135 691 681 617 420 332 276 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2021 2021 Size: 7171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 2027 2127 2298 4498 1872 Size: 155 100 171 2200 4545 Fragments arranged by size: 4545 2200 171 155 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 5275 6176 6438 820 Size: 197 926 3332 901 262 1553 Fragments arranged by size: 3332 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 3826 152 Size: 3674 3497 SphI G_CATG'C Cuts at: 1132 2740 4529 1132 Size: 1608 1789 3774 Fragments arranged by size: 3774 1789 1608 SspI AAT'ATT Cuts at: 5 52 5095 5 Size: 47 5043 2081 Fragments arranged by size: 5043 2081 47 StuI AGG'CCT Cuts at: 1517 3264 3909 1517 Size: 1747 645 4779 Fragments arranged by size: 4779 1747 645 StyI C'CwwG_G Cuts at: 513 1065 2412 2517 2562 4021 4610 513 Size: 552 1347 105 45 1459 589 3074 Fragments arranged by size: 3074 1459 1347 589 552 105 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 3818 5198 5215 5368 5553 5998 Size: 1255 1215 1023 1380 17 153 185 445 Cuts at: 5998 325 Size: 1498 Fragments arranged by size: 1498 1380 1255 1215 1023 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 5417 7129 Size: 274 80 33 51 1443 522 2918 1712 Cuts at: 7129 96 Size: 138 Fragments arranged by size: 2918 1712 1443 522 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7131 7163 7131 Size: 32 7139 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7171 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 2025 2125 2296 4496 1870 Size: 155 100 171 2200 4545 Fragments arranged by size: 4545 2200 171 155 100 XmnI GAAnn'nnTTC Cuts at: 5300 5300 Size: 7171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SanDI SapI SfoI SrfI SwaI XcmI