(Circular) (Six-base) MAPSORT of: pFN21AE1012.seq Check: 4818 from: 1 to: 5611 pFN21AE1012 5611 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2650 2686 2650 Size: 36 5575 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3674 5058 5326 1632 Size: 2042 1384 268 1917 Fragments arranged by size: 2042 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 4510 5487 1203 Size: 3307 977 1327 Fragments arranged by size: 3307 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2724 2832 1184 Size: 1540 108 3963 Fragments arranged by size: 3963 1540 108 AlwNI CAG_nnn'CTG Cuts at: 2395 2520 3354 4926 2395 Size: 125 834 1572 3080 Fragments arranged by size: 3080 1572 834 125 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 5611 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2186 2920 3146 3298 5407 1493 Size: 693 734 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 693 226 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2081 2305 2936 1870 Size: 73 125 13 224 631 4545 Fragments arranged by size: 4545 631 224 125 73 13 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2598 2932 4387 618 Size: 325 217 627 811 334 1455 1842 Fragments arranged by size: 1842 1455 811 627 334 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2357 2930 1391 Size: 671 295 573 4072 Fragments arranged by size: 4072 671 573 295 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2305 928 Size: 971 406 4234 Fragments arranged by size: 4234 971 406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4222 136 Size: 107 122 71 631 605 2550 1525 Fragments arranged by size: 2550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 2062 2601 2620 3672 4828 1790 Size: 272 539 19 1052 1156 2573 Fragments arranged by size: 2573 1156 1052 539 272 19 BmgBI CAC'GTC Cuts at: 1318 2824 1318 Size: 1506 4105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 2722 3342 Size: 416 53 83 399 24 778 527 620 Cuts at: 3342 4300 442 Size: 958 1753 Fragments arranged by size: 1753 958 778 620 527 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2270 2279 2481 2603 4271 1239 Size: 1001 30 9 202 122 1668 2579 Fragments arranged by size: 2579 1668 1001 202 122 30 9 Bpu10I CC'TnA_GC Cuts at: 2213 2213 Size: 5611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3736 4601 4899 3736 Size: 865 298 4448 Fragments arranged by size: 4448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2437 2531 4274 882 Size: 617 938 94 1743 2219 Fragments arranged by size: 2219 1743 938 617 94 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3418 3800 4435 275 Size: 53 83 186 936 1885 382 635 1451 Fragments arranged by size: 1885 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5386 Fragments arranged by size: 5386 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2365 2705 1186 Size: 1179 340 4092 Fragments arranged by size: 4092 1179 340 BseYI C'CCAG_C Cuts at: 2369 2541 2545 2626 4814 5310 2369 Size: 172 4 81 2188 496 2670 Fragments arranged by size: 2670 2188 496 172 81 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 2846 1163 Size: 573 984 126 3928 Fragments arranged by size: 3928 984 573 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2103 2322 3061 3822 4850 1540 Size: 275 186 102 219 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 275 219 186 102 BsiHKAI G_wGCw'C Cuts at: 2708 3672 3757 4828 2708 Size: 964 85 1071 3491 Fragments arranged by size: 3491 1071 964 85 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 BsmBI CGTCTCn'nnnn_ Cuts at: 2106 2106 Size: 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2357 2601 2620 2708 2930 3672 Size: 399 272 295 244 19 88 222 742 Cuts at: 3672 3757 4828 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 272 244 222 88 85 19 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2650 2686 2966 844 Size: 1806 36 280 3489 Fragments arranged by size: 3489 1806 280 36 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3498 1394 Size: 429 272 1403 3507 Fragments arranged by size: 3507 1403 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2321 2644 4255 4442 1700 Size: 110 292 219 323 1611 187 2869 Fragments arranged by size: 2869 1611 323 292 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 2036 2118 1925 Size: 111 82 5418 Fragments arranged by size: 5418 111 82 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5611 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2369 2800 2011 Size: 358 431 4822 Fragments arranged by size: 4822 431 358 BstYI r'GATC_y Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606 Size: 969 367 385 17 1452 86 12 346 Cuts at: 5606 1972 Size: 1977 Fragments arranged by size: 1977 1452 969 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2278 2787 513 Size: 552 939 274 509 3337 Fragments arranged by size: 3337 939 552 509 274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2539 2829 487 Size: 683 56 1313 290 3269 Fragments arranged by size: 3269 1313 683 290 56 BtsI GCAGTG_nn' Cuts at: 2706 3127 3921 3941 2706 Size: 421 794 20 4376 Fragments arranged by size: 4376 794 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2681 4618 809 Size: 507 1365 1937 1802 Fragments arranged by size: 1937 1802 1365 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2319 2736 Size: 54 1425 50 275 18 270 219 417 Cuts at: 2736 2790 3058 3947 5576 8 Size: 54 268 889 1629 43 Fragments arranged by size: 1629 1425 889 417 275 270 268 219 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2100 2319 3058 1537 Size: 275 288 219 739 4090 Fragments arranged by size: 4090 739 288 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 3541 1353 Size: 1289 899 3423 Fragments arranged by size: 3423 1289 899 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 2124 2425 2519 2624 2663 Size: 1064 138 512 23 301 94 105 39 Cuts at: 2663 4198 4572 4718 387 Size: 1535 374 146 1280 Fragments arranged by size: 1535 1280 1064 512 374 301 146 138 105 94 39 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2270 2279 2481 2603 3674 4271 Size: 393 608 30 9 202 122 1071 597 Cuts at: 4271 5058 5326 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1071 787 608 597 393 268 202 122 30 9 EcoO109I rG'GnC_Cy Cuts at: 2058 2653 2058 Size: 595 5016 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FseI GG_CCGG'CC Cuts at: 2325 2325 Size: 5611 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2012 2128 4758 1678 Size: 334 116 2630 2531 Fragments arranged by size: 2630 2531 334 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373 Size: 32 135 32 2680 32 42 32 2626 Fragments arranged by size: 2680 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 4725 4909 1305 Size: 1137 2283 184 2007 Fragments arranged by size: 2283 2007 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 10 Size: 54 1425 343 906 2883 Fragments arranged by size: 2883 1425 906 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3569 3928 4087 518 Size: 648 2403 359 159 2042 Fragments arranged by size: 2403 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 2280 2459 2545 2626 2761 3027 3704 Size: 534 140 179 86 81 135 266 677 Cuts at: 3704 4852 5097 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 266 245 179 140 135 86 81 NaeI GCC'GGC Cuts at: 2104 2323 2646 2104 Size: 219 323 5069 Fragments arranged by size: 5069 323 219 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4120 Fragments arranged by size: 4120 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NgoMIV G'CCGG_C Cuts at: 2102 2321 2644 2102 Size: 219 323 5069 Fragments arranged by size: 5069 323 219 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2969 4514 1132 Size: 97 1740 1545 2229 Fragments arranged by size: 2229 1740 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 3985 PfoI T'CCnGG_A Cuts at: 2685 2685 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 5611 PstI C_TGCA'G Cuts at: 830 2625 2661 2877 2963 830 Size: 1795 36 216 86 3478 Fragments arranged by size: 3478 1795 216 86 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2545 2626 2761 2545 Size: 81 135 5395 Fragments arranged by size: 5395 135 81 RsrII CG'GwC_CG Cuts at: 2299 2299 Size: 5611 SacII CC_GC'GG Cuts at: 2281 2281 Size: 5611 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2642 2642 Size: 5611 SbfI CC_TGCA'GG Cuts at: 2661 2963 2661 Size: 302 5309 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SexAI A'CCwGG_T Cuts at: 2421 2676 2421 Size: 255 5356 SfcI C'TryA_G Cuts at: 826 1045 2621 2657 2873 2959 4094 4775 4966 Size: 219 1576 36 216 86 1135 681 191 Cuts at: 4966 826 Size: 1471 Fragments arranged by size: 1576 1471 1135 681 219 216 191 86 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 2644 1700 Size: 944 4667 SmaI CCC'GGG Cuts at: 1872 2070 2307 2938 1872 Size: 198 237 631 4545 Fragments arranged by size: 4545 631 237 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 3715 4616 4878 820 Size: 197 926 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5611 StyI C'CwwG_G Cuts at: 513 1065 2004 2766 3050 513 Size: 552 939 762 284 3074 Fragments arranged by size: 3074 939 762 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 3655 3808 3993 4438 325 Size: 1255 2058 17 153 185 445 1498 Fragments arranged by size: 2058 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 3857 5569 Size: 274 80 33 51 1443 903 977 1712 Cuts at: 5569 96 Size: 138 Fragments arranged by size: 1712 1443 977 903 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2425 2011 Size: 414 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2068 2305 2936 1870 Size: 198 237 631 4545 Fragments arranged by size: 4545 631 237 198 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SanDI SfiI SfoI SrfI SwaI