Restriction map for
pFN21AE1076
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrFI BssHII
BssKI Bst1107I BstAPI BstBI BstNI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MluI MroI MspI
MvaI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScrFI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AhdI 716 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 776 AGCT AG^CT
AocI 823 CCTGAGG CC^T[ACGT]AGG
ApaI 399 GGGCCC GGGCC^C
AspEI 716 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 624 GAGCAC G[AT]GC[AT]^C
AvaII 731 GGACC G^G[AT]CC
BalI 296 TGGCCA TGG^CCA
BanII 399 GGGCCC G[GA]GC[CT]^C
BbsI 160 GAAGAC GAAGAC(2/6)
BfaI 448 CTAG C^TAG
BpuAI 160 GAAGAC GAAGAC(2/6)
BsiHKAI 624 GAGCAC G[AT]GC[AT]^C
BsmFI 455 GGGAC GGGAC(10/14)
Bsp1407I 556 TGTACA T^GTACA
BsrBI 130 CCGCTC CCGCTC(-3/-3)
BsrDI 685 GCAATG GCAATG(2/0)
BsrGI 556 TGTACA T^GTACA
BsrI 185 ACTGG ACTGG(1/-1)
BstEII 166 GGTAACC G^GT[ACGT]ACC
BstF5I 582 GGATG GGATG(2/0)
BstPI 166 GGTAACC G^GT[ACGT]ACC
BstXI 248 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 823 CCTGAGG CC^T[ACGT]AGG
DraII 399 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 457 GACAAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 138 CCACGG C^C[GA][CT]GG
Eam1105I 716 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 823 CCTGAGG CC^T[ACGT]AGG
EcoO109I 399 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 166 GGTAACC G^GT[ACGT]ACC
EcoT14I 669 CCAAGG C^C[AT][AT]GG
FauI 786 GCGGG CCCGC(4/6)
FokI 582 GGATG GGATG(9/13)
HgaI 590 GACGC GACGC(5/10)
MaeI 448 CTAG C^TAG
MaeII 376 ACGT A^CGT
MfeI 492 CAATTG C^AATTG
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MspA1I 784 CAGCGG C[AC]G^C[GT]G
MunI 492 CAATTG C^AATTG
ScaI 416 AGTACT AGT^ACT
SspBI 556 TGTACA T^GTACA
StyI 669 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 567 AAATTT [GA]^AATT[CT]
760 GAATTT
ApoI 567 AAATTT [GA]^AATT[CT]
760 GAATTT
BanI 12 GGCACC G^G[CT][GA]CC
39 GGTGCC
BbvI 831 GCAGC GCAGC(8/12)
100 GCTGC
BmyI 399 GGGCCC G[AGT]GC[ACT]^C
624 GAGCAC
BpmI 186 CTGGAG CTGGAG(16/14)
809 CTGGAG
BsaJI 138 CCACGG C^C[ACGT][ACGT]GG
669 CCAAGG
BseRI 742 GAGGAG GAGGAG(10/8)
826 GAGGAG
BsiYI 78 CCTTCTGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
390 CCTATATATGG
BslI 78 CCTTCTGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
390 CCTATATATGG
BsmAI 812 GAGAC GTCTC(1/5)
724 GTCTC
Bsp1286I 399 GGGCCC G[AGT]GC[ACT]^C
624 GAGCAC
BssSI 28 CTCGTG CACGAG(-5/-1)
112 CTCGTG
BstYI 200 AGATCC [GA]^GATC[CT]
244 AGATCC
Cac8I 60 GCTCGC GC[ACGT]^[ACGT]GC
786 GCGGGC
EaeI 296 TGGCCA [CT]^GGCC[GA]
694 CGGCCA
HphI 631 TCACC GGTGA(8/7)
638 TCACC
MflI 200 AGATCC [GA]^GATC[CT]
244 AGATCC
MwoI 4 GCGGTCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
777 GCTATACCAGC
TseI 100 GCTGC G^C[AT]GC
831 GCAGC
Tsp45I 176 GTGAC ^GT[GC]AC
466 GTCAC
XhoII 200 AGATCC [GA]^GATC[CT]
244 AGATCC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI AfaI AlwI BstUI
Cfr13I DdeI DpnII Fnu4HI HaeIII
ItaI MaeIII MboI MboII MnlI
MseI MslI MvnI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I Tru9I
Tsp509I TspRI