Restriction map for
pFN21AB5059
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BbvCI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsgI
BsiHKAI BsiWI BsmFI BspDI BspEI
BspHI BspLU11I Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Aor51HI 1647 AGCGCT AGC^GCT
ApaI 769 GGGCCC GGGCC^C
AvaII 1638 GGACC G^G[AT]CC
BalI 953 TGGCCA TGG^CCA
BbeI 965 GGCGCC GGCGC^C
BcgI 42 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 524 GTATCC GTATCC(6/5)
BsaBI 663 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 922 GAGACC GGTCTC(1/5)
BsaWI 1553 ACCGGA [AT]^CCGG[AT]
BsiEI 1827 CGGTCG CG[GA][CT]^CG
BsmBI 518 GAGACG CGTCTC(1/5)
BsrBI 63 CCGCTC CCGCTC(-3/-3)
BsrDI 1699 CATTGC GCAATG(2/0)
BsrFI 125 GCCGGC [GA]^CCGG[CT]
BsrI 696 ACTGG ACTGG(1/-1)
BssHII 298 GCGCGC G^CGCGC
BssSI 1345 CACGAG CACGAG(-5/-1)
BstEII 746 GGTTACC G^GT[ACGT]ACC
BstPI 746 GGTTACC G^GT[ACGT]ACC
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
Eco47III 1647 AGCGCT AGC^GCT
EcoO65I 746 GGTTACC G^GT[ACGT]ACC
KasI 965 GGCGCC G^GCGCC
MamI 663 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 953 TGGCCA TGG^CCA
MscI 953 TGGCCA TGG^CCA
MseI 281 TTAA T^TAA
NaeI 125 GCCGGC GCC^GGC
NarI 965 GGCGCC GG^CGCC
NgoMIV 125 GCCGGC G^CCGGC
PflMI 501 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1006 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 833 AGTACT AGT^ACT
SfoI 965 GGCGCC GGC^GCC
SnaBI 408 TACGTA TAC^GTA
StuI 582 AGGCCT AGG^CCT
TfiI 1143 GATTC G^A[AT]TC
Tru9I 281 TTAA T^TAA
Tsp509I 1516 AATT ^AATT
Van91I 501 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 965 GGCGCC G[GA]^CG[CT]C
1336 GACGCC
BanII 29 GGGCTC G[GA]GC[CT]^C
769 GGGCCC
BbsI 158 GAAGAC GAAGAC(2/6)
1287 GAAGAC
BglI 111 GCCGTTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1420 GCCATCGAGGC
BpuAI 158 GAAGAC GAAGAC(2/6)
1287 GAAGAC
BsaAI 97 TACGTG [CT]AC^GT[GA]
408 TACGTA
BsaHI 965 GGCGCC G[GA]^CG[CT]C
1336 GACGCC
BsmAI 518 GAGAC GTCTC(1/5)
922 GAGAC
BsmI 599 GCATTC GAATGC(1/-1)
1429 GCATTC
Bsp1407I 939 TGTACA T^GTACA
1548 TGTACA
BsrGI 939 TGTACA T^GTACA
1548 TGTACA
BstXI 1184 CCATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1478 CCAAGGGCCTGG
BstYI 344 AGATCC [GA]^GATC[CT]
623 AGATCC
HgaI 161 GACGC GACGC(5/10)
1336 GACGC
Hin1I 965 GGCGCC G[GA]^CG[CT]C
1336 GACGCC
MflI 344 AGATCC [GA]^GATC[CT]
623 AGATCC
PaeR7I 1273 CTCGAG C^TCGAG
1792 CTCGAG
PvuII 325 CAGCTG CAG^CTG
432 CAGCTG
SmaI 209 CCCGGG CCC^GGG
960 CCCGGG
SspBI 939 TGTACA T^GTACA
1548 TGTACA
XhoI 1273 CTCGAG C^TCGAG
1792 CTCGAG
XhoII 344 AGATCC [GA]^GATC[CT]
623 AGATCC
XmaCI 209 CCCGGG C^CCGGG
960 CCCGGG
XmaI 209 CCCGGG C^CCGGG
960 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI BanI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BspMI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I