(Circular) (Six-base) MAPSORT of: pFN21AB5059.seq Check: 9440 from: 1 to: 6571 pFN21AB5059 6571 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3685 3685 Size: 6571 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2319 2354 2493 2612 3109 4634 6018 6286 Size: 687 35 139 119 497 1525 1384 268 Cuts at: 6286 1632 Size: 1917 Fragments arranged by size: 1917 1525 1384 687 497 268 139 119 35 AfeI AGC'GCT Cuts at: 3654 3654 Size: 6571 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 2140 2152 3118 3700 4314 5886 2140 Size: 12 966 582 614 1572 2825 Fragments arranged by size: 2825 1572 966 614 582 12 ApaI G_GGCC'C Cuts at: 2778 2778 Size: 6571 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3880 4106 4258 6367 1493 Size: 2387 226 152 2109 1697 Fragments arranged by size: 2387 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2965 3278 3580 3797 3896 1870 Size: 73 271 751 313 302 217 99 4545 Fragments arranged by size: 4545 751 313 302 271 217 99 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3064 3097 3064 Size: 33 6538 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2970 3679 3892 5347 Size: 325 217 627 263 920 709 213 1455 Cuts at: 5347 618 Size: 1842 Fragments arranged by size: 1842 1455 920 709 627 325 263 217 213 BanII G_rGCy'C Cuts at: 1391 2038 2778 3890 1391 Size: 647 740 1112 4072 Fragments arranged by size: 4072 1112 740 647 BbeI G_GCGC'C Cuts at: 2974 2974 Size: 6571 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2170 3299 928 Size: 971 271 1129 4200 Fragments arranged by size: 4200 1129 971 271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2036 2070 4762 4796 1903 Size: 34 99 34 2692 34 3678 Fragments arranged by size: 3678 2692 99 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2540 4463 5673 2540 Size: 1923 1210 3438 Fragments arranged by size: 3438 1923 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 3431 5182 Size: 107 122 71 631 605 450 1309 1751 Cuts at: 5182 136 Size: 1525 Fragments arranged by size: 1751 1525 1309 631 605 450 122 107 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 2109 2778 3172 4632 5788 1790 Size: 319 669 394 1460 1156 2573 Fragments arranged by size: 2573 1460 1156 669 394 319 BmgBI CAC'GTC Cuts at: 1318 3337 3514 1318 Size: 2019 177 4375 Fragments arranged by size: 4375 2019 177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4302 5260 442 Size: 416 53 83 399 24 2885 958 1753 Fragments arranged by size: 2885 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2375 2855 5231 1239 Size: 890 246 480 2376 2579 Fragments arranged by size: 2579 2376 890 480 246 Bpu10I CC'TnA_GC Cuts at: 2403 2403 Size: 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3350 4696 5561 5859 3350 Size: 1346 865 298 4062 Fragments arranged by size: 4062 1346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2921 5234 882 Size: 617 1422 2313 2219 Fragments arranged by size: 2313 2219 1422 617 BsaAI yAC'GTr Cuts at: 493 1204 2104 2415 4362 493 Size: 711 900 311 1947 2702 Fragments arranged by size: 2702 1947 900 711 311 BsaBI GATnn'nnATC Cuts at: 2672 6565 2672 Size: 3893 2678 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2971 3342 4378 4760 Size: 53 83 186 936 1438 371 1036 382 Cuts at: 4760 5395 275 Size: 635 1451 Fragments arranged by size: 1451 1438 1036 936 635 382 371 186 83 53 BsaWI w'CCGG_w Cuts at: 3558 5004 5402 5676 5823 6234 3558 Size: 1446 398 274 147 411 3895 Fragments arranged by size: 3895 1446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3464 3494 1178 Size: 30 165 30 2061 30 4255 Fragments arranged by size: 4255 2061 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 3269 3551 3750 1186 Size: 838 1245 282 199 4007 Fragments arranged by size: 4007 1245 838 282 199 BseYI C'CCAG_C Cuts at: 3206 5774 6270 3206 Size: 2568 496 3507 Fragments arranged by size: 3507 2568 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5998 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3835 4021 4782 5810 1540 Size: 275 186 1834 186 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 186 BsiHKAI G_wGCw'C Cuts at: 4632 4717 5788 4632 Size: 85 1071 5415 Fragments arranged by size: 5415 1071 85 BsmI GAATG_Cn' Cuts at: 2604 3434 4082 4175 2604 Size: 830 648 93 5000 Fragments arranged by size: 5000 830 648 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2517 2517 Size: 6571 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2109 2778 3172 3890 4632 4717 Size: 399 248 71 669 394 718 742 85 Cuts at: 4717 5788 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 718 669 399 394 248 85 71 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 4460 6190 4460 Size: 1730 4841 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3282 3366 3685 3808 3926 844 Size: 2438 84 319 123 118 3489 Fragments arranged by size: 3489 2438 319 123 118 84 BsrBI CCG'CTC Cuts at: 1394 1823 2070 4458 1394 Size: 429 247 2388 3507 Fragments arranged by size: 3507 2388 429 247 BsrDI GCAATG_nn' Cuts at: 65 1248 3703 5066 5240 65 Size: 1183 2455 1363 174 1396 Fragments arranged by size: 2455 1396 1363 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 5215 5402 1700 Size: 110 320 3085 187 2869 Fragments arranged by size: 3085 2869 320 187 110 BsrGI T'GTAC_A Cuts at: 96 2944 3553 96 Size: 2848 609 3114 Fragments arranged by size: 3114 2848 609 BssHII G'CGCG_C Cuts at: 1925 2303 1925 Size: 378 6193 BssSI C'ACGA_G Cuts at: 3350 4631 5643 3350 Size: 1281 1012 4278 Fragments arranged by size: 4278 1281 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 3679 3700 794 Size: 1346 1539 21 3665 Fragments arranged by size: 3665 1539 1346 21 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 2751 1184 Size: 1567 5004 BstXI CCAn_nnnn'nTGG Cuts at: 3196 3490 3196 Size: 294 6277 BstYI r'GATC_y Cuts at: 1972 2349 2628 3901 4268 4653 4670 6122 6208 Size: 377 279 1273 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1273 385 377 367 346 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2824 3391 3861 513 Size: 552 939 820 567 470 3223 Fragments arranged by size: 3223 939 820 567 552 470 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2659 3580 487 Size: 683 56 1433 921 3478 Fragments arranged by size: 3478 1433 921 683 56 BtsI GCAGTG_nn' Cuts at: 4087 4881 4901 4087 Size: 794 20 5757 Fragments arranged by size: 5757 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3457 3492 529 Size: 35 2893 35 3608 Fragments arranged by size: 3608 2893 35 35 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2656 2958 3565 Size: 54 1425 50 275 18 826 302 607 Cuts at: 3565 4018 4907 6536 8 Size: 453 889 1629 43 Fragments arranged by size: 1629 1425 889 826 607 453 302 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 2337 2469 2741 3128 4501 1353 Size: 828 156 132 272 387 1373 3423 Fragments arranged by size: 3423 1373 828 387 272 156 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 5158 5532 5678 387 Size: 1064 138 504 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 504 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2319 2354 2375 2493 2612 2855 Size: 393 497 190 35 21 118 119 243 Cuts at: 2855 3109 4634 5231 6018 6286 1239 Size: 254 1525 597 787 268 1524 Fragments arranged by size: 1525 1524 787 597 497 393 268 254 243 190 119 118 35 21 EcoNI CCTnn'n_nnAGG Cuts at: 2058 2516 2855 3035 2058 Size: 458 339 180 5594 Fragments arranged by size: 5594 458 339 180 EcoO109I rG'GnC_Cy Cuts at: 2243 2774 2775 3487 3763 2243 Size: 531 1 712 276 5051 Fragments arranged by size: 5051 712 531 276 1 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 2131 2974 3580 3656 5718 1678 Size: 453 843 606 76 2062 2531 Fragments arranged by size: 2531 2062 843 606 453 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2514 2546 2775 2807 3464 Size: 32 135 32 942 32 229 32 657 Cuts at: 3464 3496 5212 5244 5286 5318 1373 Size: 32 1716 32 42 32 2626 Fragments arranged by size: 2626 1716 942 657 229 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3915 4161 669 Size: 988 281 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KasI G'GCGC_C Cuts at: 2970 2970 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1995 2277 2959 5685 5869 1305 Size: 690 282 682 2726 184 2007 Fragments arranged by size: 2726 2007 690 682 282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 10 Size: 54 1425 343 1128 3621 Fragments arranged by size: 3621 1425 1128 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2909 2954 3194 4529 4888 5047 Size: 648 1347 396 45 240 1335 359 159 Cuts at: 5047 518 Size: 2042 Fragments arranged by size: 2042 1347 1335 648 396 359 240 159 45 MspA1I CmG'CkG Cuts at: 1606 2202 2332 2439 3987 4664 5812 6057 1606 Size: 596 130 107 1548 677 1148 245 2120 Fragments arranged by size: 2120 1548 1148 677 596 245 130 107 NaeI GCC'GGC Cuts at: 2132 2132 Size: 6571 NarI GG'CG_CC Cuts at: 2971 2971 Size: 6571 NcoI C'CATG_G Cuts at: 513 1065 2004 2824 3391 3861 513 Size: 552 939 820 567 470 3223 Fragments arranged by size: 3223 939 820 567 552 470 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NgoMIV G'CCGG_C Cuts at: 2130 2130 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 2470 2908 3532 3929 5474 1132 Size: 97 1241 438 624 397 1545 2229 Fragments arranged by size: 2229 1545 1241 624 438 397 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 1108 Size: 1404 5167 PfoI T'CCnGG_A Cuts at: 2265 2352 2832 3231 3438 2265 Size: 87 480 399 207 5398 Fragments arranged by size: 5398 480 399 207 87 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3015 1613 Size: 1402 5169 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 2774 2774 Size: 6571 PspXI vC'TCGA_Gb Cuts at: 3278 3797 3278 Size: 519 6052 PstI C_TGCA'G Cuts at: 830 2160 2575 2859 2991 3291 3696 3923 830 Size: 1330 415 284 132 300 405 227 3478 Fragments arranged by size: 3478 1330 415 405 300 284 227 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2332 2439 2332 Size: 107 6464 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SapI GCTCTTCn'nnn_ Cuts at: 2181 2469 3128 2181 Size: 288 659 5624 Fragments arranged by size: 5624 659 288 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 2840 4819 6531 2840 Size: 1979 1712 2880 Fragments arranged by size: 2880 1979 1712 SfcI C'TryA_G Cuts at: 826 1045 2156 2571 2855 2987 3287 3692 3919 Size: 219 1111 415 284 132 300 405 227 Cuts at: 3919 5054 5735 5926 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 415 405 300 284 227 219 191 132 SfoI GGC'GCC Cuts at: 2972 2972 Size: 6571 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 2216 2967 3898 1872 Size: 344 751 931 4545 Fragments arranged by size: 4545 931 751 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 3278 3365 3797 4675 5576 5838 Size: 197 926 1335 87 432 878 901 262 Cuts at: 5838 820 Size: 1553 Fragments arranged by size: 1553 1335 926 901 878 432 262 197 87 SnaBI TAC'GTA Cuts at: 493 2415 4362 493 Size: 1922 1947 2702 Fragments arranged by size: 2702 1947 1922 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 5499 StyI C'CwwG_G Cuts at: 513 1065 2004 2319 2824 3391 3483 3861 4010 Size: 552 939 315 505 567 92 378 149 Cuts at: 4010 513 Size: 3074 Fragments arranged by size: 3074 939 567 552 505 378 315 149 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 2628 2635 3821 4598 4615 4768 Size: 1255 974 74 7 1186 777 17 153 Cuts at: 4768 4953 5398 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1186 974 777 445 185 153 74 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2838 2944 3307 Size: 274 80 33 51 1443 861 106 363 Cuts at: 3307 3553 4817 6529 96 Size: 246 1264 1712 138 Fragments arranged by size: 1712 1443 1264 861 363 274 246 138 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6531 6563 6531 Size: 32 6539 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 3278 3797 1943 Size: 1335 519 4717 Fragments arranged by size: 4717 1335 519 XmaI C'CCGG_G Cuts at: 1870 2214 2965 3896 1870 Size: 344 751 931 4545 Fragments arranged by size: 4545 931 751 344 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI