Restriction map for
pFN21AB5059
(Linear) (Six-base) MAPSORT of: KIBB5059.seq Check: 5634 from: 1 to: 1866
KIBB5059.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1680 1866
Size: 1680 186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 349 488 607 1104 1866
Size: 314 35 139 119 497 762
Fragments arranged by size:
762 497 314 139 119 35
AfeI AGC'GCT
Cuts at: 0 1649 1866
Size: 1649 217
AlwNI CAG_nnn'CTG
Cuts at: 0 135 147 1113 1695 1866
Size: 135 12 966 582 171
Fragments arranged by size:
966 582 171 135 12
ApaI G_GGCC'C
Cuts at: 0 773 1866
Size: 773 1093
AvaI C'yCGr_G
Cuts at: 0 209 960 1273 1575 1792 1866
Size: 209 751 313 302 217 74
Fragments arranged by size:
751 313 302 217 209 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1059 1092 1866
Size: 1059 33 774
Fragments arranged by size:
1059 774 33
BanI G'GyrC_C
Cuts at: 0 45 965 1674 1866
Size: 45 920 709 192
Fragments arranged by size:
920 709 192 45
BanII G_rGCy'C
Cuts at: 0 33 773 1866
Size: 33 740 1093
Fragments arranged by size:
1093 740 33
BbeI G_GCGC'C
Cuts at: 0 969 1866
Size: 969 897
BbsI GAAGACnn'nnnn_
Cuts at: 0 165 1294 1866
Size: 165 1129 572
Fragments arranged by size:
1129 572 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 65 1866
Size: 31 34 1801
Fragments arranged by size:
1801 34 31
BciVI GTATCCnnnnn_n'
Cuts at: 0 535 1866
Size: 535 1331
BglI GCCn_nnn'nGGC
Cuts at: 0 117 1426 1866
Size: 117 1309 440
Fragments arranged by size:
1309 440 117
Bme1580I G_kGCm'C
Cuts at: 0 104 773 1167 1866
Size: 104 669 394 699
Fragments arranged by size:
699 669 394 104
BmgBI CAC'GTC
Cuts at: 0 1332 1509 1866
Size: 1332 177 357
Fragments arranged by size:
1332 357 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 879 1866
Size: 879 987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 370 850 1866
Size: 124 246 480 1016
Fragments arranged by size:
1016 480 246 124
Bpu10I CC'TnA_GC
Cuts at: 0 398 1866
Size: 398 1468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1866
Size: 1345 521
BsaI GGTCTCn'nnnn_
Cuts at: 0 916 1866
Size: 916 950
BsaAI yAC'GTr
Cuts at: 0 99 410 1866
Size: 99 311 1456
Fragments arranged by size:
1456 311 99
BsaBI GATnn'nnATC
Cuts at: 0 667 1866
Size: 667 1199
BsaHI Gr'CG_yC
Cuts at: 0 966 1337 1866
Size: 966 371 529
Fragments arranged by size:
966 529 371
BsaWI w'CCGG_w
Cuts at: 0 1553 1866
Size: 1553 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1459 1489 1866
Size: 1459 30 377
Fragments arranged by size:
1459 377 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1264 1546 1745 1866
Size: 19 1245 282 199 121
Fragments arranged by size:
1245 282 199 121 19
BseYI C'CCAG_C
Cuts at: 0 1201 1866
Size: 1201 665
BsiEI CG_ry'CG
Cuts at: 0 1830 1866
Size: 1830 36
BsmI GAATG_Cn'
Cuts at: 0 599 1429 1866
Size: 599 830 437
Fragments arranged by size:
830 599 437
BsmBI CGTCTCn'nnnn_
Cuts at: 0 512 1866
Size: 512 1354
Bsp1286I G_dGCh'C
Cuts at: 0 33 104 773 1167 1866
Size: 33 71 669 394 699
Fragments arranged by size:
699 669 394 71 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1277 1361 1680 1803 1866
Size: 1277 84 319 123 63
Fragments arranged by size:
1277 319 123 84 63
BsrBI CCG'CTC
Cuts at: 0 65 1866
Size: 65 1801
BsrDI GCAATG_nn'
Cuts at: 0 1698 1866
Size: 1698 168
BsrFI r'CCGG_y
Cuts at: 0 125 1866
Size: 125 1741
BsrGI T'GTAC_A
Cuts at: 0 939 1548 1866
Size: 939 609 318
Fragments arranged by size:
939 609 318
BssHII G'CGCG_C
Cuts at: 0 298 1866
Size: 298 1568
BssSI C'ACGA_G
Cuts at: 0 1345 1866
Size: 1345 521
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 1674 1695 1866
Size: 135 1539 21 171
Fragments arranged by size:
1539 171 135 21
BstEII G'GTnAC_C
Cuts at: 0 746 1866
Size: 746 1120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1191 1485 1866
Size: 1191 294 381
Fragments arranged by size:
1191 381 294
BstYI r'GATC_y
Cuts at: 0 344 623 1866
Size: 344 279 1243
Fragments arranged by size:
1243 344 279
BtgI C'CryG_G
Cuts at: 0 819 1386 1856 1866
Size: 819 567 470 10
Fragments arranged by size:
819 567 470 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 654 1575 1866
Size: 654 921 291
Fragments arranged by size:
921 654 291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1452 1487 1866
Size: 1452 35 379
Fragments arranged by size:
1452 379 35
EaeI y'GGCC_r
Cuts at: 0 651 953 1560 1866
Size: 651 302 607 306
Fragments arranged by size:
651 607 306 302
EarI CTCTTCn'nnn_
Cuts at: 0 176 332 464 736 1123 1866
Size: 176 156 132 272 387 743
Fragments arranged by size:
743 387 272 176 156 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 1866
Size: 88 1778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 314 349 370 488 607 850 1104
Size: 124 190 35 21 118 119 243 254
Cuts at: 1104 1866
Size: 762
Fragments arranged by size:
762 254 243 190 124 119 118 35
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 511 850 1030 1866
Size: 53 458 339 180 836
Fragments arranged by size:
836 458 339 180 53
EcoO109I rG'GnC_Cy
Cuts at: 0 238 769 770 1482 1758 1866
Size: 238 531 1 712 276 108
Fragments arranged by size:
712 531 276 238 108 1
HaeII r_GCGC'y
Cuts at: 0 126 969 1575 1651 1866
Size: 126 843 606 76 215
Fragments arranged by size:
843 606 215 126 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 770 802 1459 1491 1866
Size: 509 32 229 32 657 32 375
Fragments arranged by size:
657 509 375 229 32 32 32
KasI G'GCGC_C
Cuts at: 0 965 1866
Size: 965 901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 954 1866
Size: 272 682 912
Fragments arranged by size:
912 682 272
MscI TGG'CCA
Cuts at: 0 955 1866
Size: 955 911
MslI CAynn'nnrTG
Cuts at: 0 508 904 949 1189 1866
Size: 508 396 45 240 677
Fragments arranged by size:
677 508 396 240 45
MspA1I CmG'CkG
Cuts at: 0 197 327 434 1866
Size: 197 130 107 1432
Fragments arranged by size:
1432 197 130 107
NaeI GCC'GGC
Cuts at: 0 127 1866
Size: 127 1739
NarI GG'CG_CC
Cuts at: 0 966 1866
Size: 966 900
NcoI C'CATG_G
Cuts at: 0 819 1386 1856 1866
Size: 819 567 470 10
Fragments arranged by size:
819 567 470 10
NgoMIV G'CCGG_C
Cuts at: 0 125 1866
Size: 125 1741
NspI r_CATG'y
Cuts at: 0 465 903 1527 1866
Size: 465 438 624 339
Fragments arranged by size:
624 465 438 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 1866
Size: 507 1359
PfoI T'CCnGG_A
Cuts at: 0 260 347 827 1226 1433 1866
Size: 260 87 480 399 207 433
Fragments arranged by size:
480 433 399 260 207 87
PshAI GACnn'nnGTC
Cuts at: 0 1010 1866
Size: 1010 856
PspOMI G'GGCC_C
Cuts at: 0 769 1866
Size: 769 1097
PspXI vC'TCGA_Gb
Cuts at: 0 1273 1792 1866
Size: 1273 519 74
Fragments arranged by size:
1273 519 74
PstI C_TGCA'G
Cuts at: 0 155 570 854 986 1286 1691 1866
Size: 155 415 284 132 300 405 175
Fragments arranged by size:
415 405 300 284 175 155 132
PvuII CAG'CTG
Cuts at: 0 327 434 1866
Size: 327 107 1432
Fragments arranged by size:
1432 327 107
SapI GCTCTTCn'nnn_
Cuts at: 0 176 464 1123 1866
Size: 176 288 659 743
Fragments arranged by size:
743 659 288 176
ScaI AGT'ACT
Cuts at: 0 835 1866
Size: 835 1031
SfcI C'TryA_G
Cuts at: 0 151 566 850 982 1282 1687 1866
Size: 151 415 284 132 300 405 179
Fragments arranged by size:
415 405 300 284 179 151 132
SfoI GGC'GCC
Cuts at: 0 967 1866
Size: 967 899
SmaI CCC'GGG
Cuts at: 0 211 962 1866
Size: 211 751 904
Fragments arranged by size:
904 751 211
SmlI C'TyrA_G
Cuts at: 0 1273 1360 1792 1866
Size: 1273 87 432 74
Fragments arranged by size:
1273 432 87 74
SnaBI TAC'GTA
Cuts at: 0 410 1866
Size: 410 1456
StuI AGG'CCT
Cuts at: 0 584 1866
Size: 584 1282
StyI C'CwwG_G
Cuts at: 0 314 819 1386 1478 1856 1866
Size: 314 505 567 92 378 10
Fragments arranged by size:
567 505 378 314 92 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 623 630 1816 1866
Size: 549 74 7 1186 50
Fragments arranged by size:
1186 549 74 50 7
TatI w'GTAC_w
Cuts at: 0 833 939 1302 1548 1866
Size: 833 106 363 246 318
Fragments arranged by size:
833 363 318 246 106
XhoI C'TCGA_G
Cuts at: 0 1273 1792 1866
Size: 1273 519 74
Fragments arranged by size:
1273 519 74
XmaI C'CCGG_G
Cuts at: 0 209 960 1866
Size: 209 751 906
Fragments arranged by size:
906 751 209
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BaeI BanI
BanII BbeI BbsI BcgI BciVI BglI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI CspCI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PfoI PshAI PspOMI PspXI PstI PvuII SapI
ScaI SfcI SfoI SmaI SmlI SnaBI StuI StyI
TaqII TatI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbvCI BclI BfrBI BglII BlpI BmrI
BmtI BsgI BsiHKAI BsiWI BspEI BspHI BstBI BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PsiI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XmnI ZraI