(Circular) (Six-base) MAPSORT of: pFN21AB3654.seq Check: 7820 from: 1 to: 7792 pFN21AB3654 7792 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5602 278 Size: 53 83 186 936 4066 2468 Fragments arranged by size: 4066 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5135 1937 Size: 3198 4594 Acc65I G'GTAC_C Cuts at: 5113 5113 Size: 7792 AclI AA'CG_TT Cuts at: 1563 5919 6292 1563 Size: 4356 373 3063 Fragments arranged by size: 4356 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2589 2670 3117 3587 4007 4427 4468 5855 Size: 957 81 447 470 420 420 41 1387 Cuts at: 5855 7239 7507 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1387 1384 957 470 447 420 420 268 81 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7595 AflIII A'CryG_T Cuts at: 1203 6691 7668 1203 Size: 5488 977 1327 Fragments arranged by size: 5488 1327 977 AgeI A'CCGG_T Cuts at: 6623 6623 Size: 7792 AhdI GACnn_n'nnGTC Cuts at: 6521 6521 Size: 7792 AleI CACnn'nnGTG Cuts at: 1166 3183 4359 1166 Size: 2017 1176 4599 Fragments arranged by size: 4599 2017 1176 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7792 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2443 2475 2443 Size: 32 7760 AlwNI CAG_nnn'CTG Cuts at: 2248 2275 5535 7107 2248 Size: 27 3260 1572 2933 Fragments arranged by size: 3260 2933 1572 27 ApaI G_GGCC'C Cuts at: 2711 2711 Size: 7792 ApaLI G'TGCA_C Cuts at: 5849 7005 5849 Size: 1156 6636 ApoI r'AATT_y Cuts at: 1493 2496 4434 4602 4834 4854 4985 5039 5101 Size: 1003 1938 168 232 20 131 54 62 Cuts at: 5101 5327 5479 7588 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1938 1697 1003 232 226 168 152 131 62 54 20 AseI AT'TA_AT Cuts at: 160 1032 2612 6346 160 Size: 872 1580 3734 1606 Fragments arranged by size: 3734 1606 1580 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7792 AvaI C'yCGr_G Cuts at: 1870 1943 5117 1870 Size: 73 3174 4545 Fragments arranged by size: 4545 3174 73 BamHI G'GATC_C Cuts at: 2542 3342 4518 5122 5489 2542 Size: 800 1176 604 367 4845 Fragments arranged by size: 4845 1176 800 604 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3426 5113 6568 618 Size: 325 217 627 1639 1687 1455 1842 Fragments arranged by size: 1842 1687 1639 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2711 5111 1391 Size: 1320 2400 4072 Fragments arranged by size: 4072 2400 1320 BbeI G_GCGC'C Cuts at: 3430 3430 Size: 7792 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3466 4054 4474 928 Size: 971 1567 588 420 4246 Fragments arranged by size: 4246 1567 971 588 420 BbvCI CC'TCA_GC Cuts at: 2547 2547 Size: 7792 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5983 6017 1903 Size: 34 4046 34 3678 Fragments arranged by size: 4046 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5684 6894 5684 Size: 1210 6582 BclI T'GATC_A Cuts at: 1548 1548 Size: 7792 BfrBI ATG'CAT Cuts at: 3250 3250 Size: 7792 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6403 136 Size: 107 122 71 631 605 4731 1525 Fragments arranged by size: 4731 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7787 7787 Size: 7792 BlpI GC'TnA_GC Cuts at: 5209 5209 Size: 7792 Bme1580I G_kGCm'C Cuts at: 1790 2446 2711 5853 7009 1790 Size: 656 265 3142 1156 2573 Fragments arranged by size: 3142 2573 1156 656 265 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7792 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 3360 4788 Size: 416 53 83 399 24 1323 620 1428 Cuts at: 4788 5523 6481 442 Size: 735 958 1753 Fragments arranged by size: 1753 1428 1323 958 735 620 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7792 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 5059 4049 Size: 1010 6782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2285 2411 2522 2765 4044 4716 6452 Size: 1031 15 126 111 243 1279 672 1736 Cuts at: 6452 1239 Size: 2579 Fragments arranged by size: 2579 1736 1279 1031 672 243 126 111 15 Bpu10I CC'TnA_GC Cuts at: 2183 2192 2547 2183 Size: 9 355 7428 Fragments arranged by size: 7428 355 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5917 6782 7080 5917 Size: 865 298 6629 Fragments arranged by size: 6629 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 2212 2774 3436 6455 882 Size: 617 615 98 562 662 3019 2219 Fragments arranged by size: 3019 2219 662 617 615 562 98 BsaAI yAC'GTr Cuts at: 493 1204 4572 5583 493 Size: 711 3368 1011 2702 Fragments arranged by size: 3368 2702 1011 711 BsaBI GATnn'nnATC Cuts at: 7786 7786 Size: 7792 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 5599 5981 6616 Size: 53 83 186 936 1894 2172 382 635 Cuts at: 6616 275 Size: 1451 Fragments arranged by size: 2172 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4274 6225 6623 6897 7044 7455 4274 Size: 1951 398 274 147 411 4611 Fragments arranged by size: 4611 1951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2446 2476 2610 2640 1178 Size: 30 165 30 1043 30 134 30 6330 Fragments arranged by size: 6330 1043 165 134 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2351 2372 2678 2813 3737 1186 Size: 912 253 21 306 135 924 5241 Fragments arranged by size: 5241 924 912 306 253 135 21 BseYI C'CCAG_C Cuts at: 2637 6995 7491 2637 Size: 4358 496 2938 Fragments arranged by size: 4358 2938 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3569 3708 1163 Size: 573 1833 139 5247 Fragments arranged by size: 5247 1833 573 139 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5242 6003 7031 1540 Size: 275 186 3241 761 1028 2301 Fragments arranged by size: 3241 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5853 5938 7009 5853 Size: 85 1071 6636 Fragments arranged by size: 6636 1071 85 BsmI GAATG_Cn' Cuts at: 2528 3490 4298 4776 5303 5396 2528 Size: 962 808 478 527 93 4924 Fragments arranged by size: 4924 962 808 527 478 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2446 2711 5111 5853 5938 7009 1391 Size: 399 656 265 2400 742 85 1071 2174 Fragments arranged by size: 2400 2174 1071 742 656 399 265 85 BspEI T'CCGG_A Cuts at: 7455 7455 Size: 7792 BspHI T'CATG_A Cuts at: 5681 7411 5681 Size: 1730 6062 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5147 844 Size: 4303 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4494 5679 1394 Size: 429 2671 1185 3507 Fragments arranged by size: 3507 2671 1185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4471 6287 6461 65 Size: 1183 3223 1816 174 1396 Fragments arranged by size: 3223 1816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 2208 6436 6623 1700 Size: 110 377 21 4228 187 2869 Fragments arranged by size: 4228 2869 377 187 110 21 BsrGI T'GTAC_A Cuts at: 96 4255 4759 4927 96 Size: 4159 504 168 2961 Fragments arranged by size: 4159 2961 504 168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7792 BssSI C'ACGA_G Cuts at: 4441 5852 6864 4441 Size: 1411 1012 5369 Fragments arranged by size: 5369 1411 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7792 BstBI TT'CG_AA Cuts at: 3733 6596 3733 Size: 2863 4929 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7792 BstXI CCAn_nnnn'nTGG Cuts at: 2324 2324 Size: 7792 BstYI r'GATC_y Cuts at: 1972 2244 2542 3342 4518 5122 5489 5874 5891 Size: 272 298 800 1176 604 367 385 17 Cuts at: 5891 7343 7429 7441 7787 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1176 800 604 385 367 346 298 272 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7053 Fragments arranged by size: 7053 683 56 BtsI GCAGTG_nn' Cuts at: 4303 5308 6102 6122 4303 Size: 1005 794 20 5973 Fragments arranged by size: 5973 1005 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7792 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4453 4488 529 Size: 35 3889 35 3833 Fragments arranged by size: 3889 3833 35 35 DraI TTT'AAA Cuts at: 5096 5096 Size: 7792 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6799 809 Size: 507 5483 1802 Fragments arranged by size: 5483 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4843 5239 6128 Size: 54 1425 50 275 18 3013 396 889 Cuts at: 6128 7757 8 Size: 1629 43 Fragments arranged by size: 3013 1629 1425 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5239 1537 Size: 275 3427 4090 Fragments arranged by size: 4090 3427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 2895 3566 3986 4303 5722 1353 Size: 1233 309 671 420 317 1419 3423 Fragments arranged by size: 3423 1419 1233 671 420 317 309 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6379 6753 6899 387 Size: 1064 138 4790 374 146 1280 Fragments arranged by size: 4790 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2285 2411 2522 2589 2670 2765 Size: 393 638 15 126 111 67 81 95 Cuts at: 2765 3117 3587 4007 4044 4427 4468 4716 5855 Size: 352 470 420 37 383 41 248 1139 Cuts at: 5855 6452 7239 7507 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1139 787 638 597 470 420 393 383 352 268 248 126 111 95 81 67 41 37 15 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2354 2408 2585 2054 Size: 300 54 177 7261 Fragments arranged by size: 7261 300 177 54 EcoO109I rG'GnC_Cy Cuts at: 2047 2721 2884 2047 Size: 674 163 6955 Fragments arranged by size: 6955 674 163 EcoRI G'AATT_C Cuts at: 4434 4602 4854 4985 5039 5101 5479 4434 Size: 168 252 131 54 62 378 6747 Fragments arranged by size: 6747 378 252 168 131 62 54 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2066 2996 3799 3844 2066 Size: 930 803 45 6014 Fragments arranged by size: 6014 930 803 45 FspI TGC'GCA Cuts at: 6298 6298 Size: 7792 HaeII r_GCGC'y Cuts at: 1678 3430 6939 1678 Size: 1752 3509 2531 Fragments arranged by size: 3509 2531 1752 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2654 2686 2792 2824 3631 Size: 32 135 32 1082 32 106 32 807 Cuts at: 3631 3643 3663 3675 4579 4611 6433 6465 6507 Size: 12 20 12 904 32 1822 32 42 Cuts at: 6507 6539 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 1082 904 807 135 106 42 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 5136 5382 669 Size: 988 281 3198 246 3079 Fragments arranged by size: 3198 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2571 3555 748 Size: 1823 984 4985 Fragments arranged by size: 4985 1823 984 HpaI GTT'AAC Cuts at: 5382 5382 Size: 7792 KasI G'GCGC_C Cuts at: 3426 3426 Size: 7792 KpnI G_GTAC'C Cuts at: 5117 5117 Size: 7792 MfeI C'AATT_G Cuts at: 5391 5391 Size: 7792 MluI A'CGCG_T Cuts at: 7668 7668 Size: 7792 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5025 6906 7090 1305 Size: 3720 1881 184 2007 Fragments arranged by size: 3720 2007 1881 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4845 10 Size: 54 1425 343 3013 2957 Fragments arranged by size: 3013 2957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3183 4123 4359 4527 4947 5750 6109 Size: 648 2017 940 236 168 420 803 359 Cuts at: 6109 6268 518 Size: 159 2042 Fragments arranged by size: 2042 2017 940 803 648 420 359 236 168 159 MspA1I CmG'CkG Cuts at: 1606 2492 5208 5885 7033 7278 1606 Size: 886 2716 677 1148 245 2120 Fragments arranged by size: 2716 2120 1148 886 677 245 NaeI GCC'GGC Cuts at: 2189 2189 Size: 7792 NarI GG'CG_CC Cuts at: 3427 3427 Size: 7792 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7240 NdeI CA'TA_TG Cuts at: 387 4120 387 Size: 3733 4059 NgoMIV G'CCGG_C Cuts at: 2187 2187 Size: 7792 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7792 NotI GC'GGCC_GC Cuts at: 5239 5239 Size: 7792 NsiI A_TGCA'T Cuts at: 3252 3252 Size: 7792 NspI r_CATG'y Cuts at: 1132 1229 2703 5150 6695 1132 Size: 97 1474 2447 1545 2229 Fragments arranged by size: 2447 2229 1545 1474 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7792 PciI A'CATG_T Cuts at: 6691 6691 Size: 7792 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 2650 1108 Size: 1326 216 6250 Fragments arranged by size: 6250 1326 216 PfoI T'CCnGG_A Cuts at: 2247 2538 2247 Size: 291 7501 PmeI GTTT'AAAC Cuts at: 5096 5096 Size: 7792 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5861 5893 7374 7406 5861 Size: 32 1481 32 6247 Fragments arranged by size: 6247 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2884 Size: 7792 PshAI GACnn'nnGTC Cuts at: 1613 4737 1613 Size: 3124 4668 PsiI TTA'TAA Cuts at: 3114 3161 3282 3366 3450 3534 4458 4710 5362 Size: 47 121 84 84 84 924 252 652 Cuts at: 5362 3114 Size: 5544 Fragments arranged by size: 5544 924 652 252 121 84 84 84 47 PspOMI G'GGCC_C Cuts at: 2707 2707 Size: 7792 PstI C_TGCA'G Cuts at: 830 2367 5144 830 Size: 1537 2777 3478 Fragments arranged by size: 3478 2777 1537 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7792 PvuII CAG'CTG Cuts at: 2492 2492 Size: 7792 SalI G'TCGA_C Cuts at: 1936 5134 1936 Size: 3198 4594 SapI GCTCTTCn'nnn_ Cuts at: 3566 3986 4303 3566 Size: 420 317 7055 Fragments arranged by size: 7055 420 317 SbfI CC_TGCA'GG Cuts at: 5144 5144 Size: 7792 ScaI AGT'ACT Cuts at: 6040 7752 6040 Size: 1712 6080 SfcI C'TryA_G Cuts at: 826 1045 2363 3394 5140 6275 6956 7147 826 Size: 219 1318 1031 1746 1135 681 191 1471 Fragments arranged by size: 1746 1471 1318 1135 1031 681 219 191 SfoI GGC'GCC Cuts at: 3428 3428 Size: 7792 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7792 SgrAI Cr'CCGG_yG Cuts at: 1700 2208 1700 Size: 508 7284 SmaI CCC'GGG Cuts at: 1872 5119 1872 Size: 3247 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5896 6797 7059 820 Size: 197 926 3953 901 262 1553 Fragments arranged by size: 3953 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4572 5583 493 Size: 4079 1011 2702 Fragments arranged by size: 4079 2702 1011 SpeI A'CTAG_T Cuts at: 152 152 Size: 7792 SphI G_CATG'C Cuts at: 1132 5150 1132 Size: 4018 3774 SspI AAT'ATT Cuts at: 5 52 5716 5 Size: 47 5664 2081 Fragments arranged by size: 5664 2081 47 StuI AGG'CCT Cuts at: 1517 3408 1517 Size: 1891 5901 StyI C'CwwG_G Cuts at: 513 1065 2095 2235 5231 513 Size: 552 1030 140 2996 3074 Fragments arranged by size: 3074 2996 1030 552 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2804 4869 5819 5836 5989 6174 6619 Size: 1255 1224 2065 950 17 153 185 445 Cuts at: 6619 325 Size: 1498 Fragments arranged by size: 2065 1498 1255 1224 950 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2467 2727 4255 Size: 274 80 33 51 1443 490 260 1528 Cuts at: 4255 4759 4927 6038 7750 96 Size: 504 168 1111 1712 138 Fragments arranged by size: 1712 1528 1443 1111 504 490 274 260 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2610 2642 7752 7784 2610 Size: 32 5110 32 2618 Fragments arranged by size: 5110 2618 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7792 XbaI T'CTAG_A Cuts at: 5128 5128 Size: 7792 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2162 Size: 7792 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7792 XmaI C'CCGG_G Cuts at: 1870 5117 1870 Size: 3247 4545 XmnI GAAnn'nnTTC Cuts at: 3473 3557 3809 4229 4481 5921 3473 Size: 84 252 420 252 1440 5344 Fragments arranged by size: 5344 1440 420 252 252 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5600 276 Size: 53 83 186 936 4066 2468 Fragments arranged by size: 4066 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI