Restriction map for
pFN21AB7268
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MluI MroI MspA1I
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflIII 1524 ACATGT A^C[GA][CT]GT
AgeI 1177 ACCGGT A^CCGGT
AhdI 1467 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 974 GGATC GGATC(4/5)
AseI 652 ATTAAT AT^TAAT
AsnI 652 ATTAAT AT^TAAT
Asp700I 352 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1467 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1227 GAGCTC G[AT]GC[AT]^C
BalI 125 TGGCCA TGG^CCA
BanI 162 GGCACC G^G[CT][GA]CC
BbvI 366 GCAGC GCAGC(8/12)
BclI 518 TGATCA T^GATCA
BsaBI 516 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1177 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1227 GAGCTC G[AT]GC[AT]^C
BsmBI 188 GAGACG CGTCTC(1/5)
BsmFI 96 GTCCC GGGAC(10/14)
BspHI 1476 TCATGA T^CATGA
BspLU11I 1524 ACATGT A^CATGT
BspMI 165 ACCTGC ACCTGC(4/8)
BsrDI 1393 CATTGC GCAATG(2/0)
BsrFI 1177 ACCGGT [GA]^CCGG[CT]
BstXI 1608 CCATATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 974 GGATCT [GA]^GATC[CT]
Cfr10I 1177 ACCGGT [GA]^CCGG[CT]
DraI 715 TTTAAA TTT^AAA
EaeI 125 TGGCCA [CT]^GGCC[GA]
Eam1105I 1467 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 518 TGATCA T^GATCA
Fnu4HI 366 GCAGC GC^[ACGT]GC
HapII 1178 CCGG C^CGG
HpaII 1178 CCGG C^CGG
ItaI 366 GCAGC GC^[ACGT]GC
MaeII 191 ACGT A^CGT
MaeIII 1565 GTTAC ^GT[ACGT]AC
MamI 516 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1402 CAATTG C^AATTG
MflI 974 GGATCT [GA]^GATC[CT]
MluNI 125 TGGCCA TGG^CCA
MscI 125 TGGCCA TGG^CCA
MspI 1178 CCGG C^CGG
MunI 1402 CAATTG C^AATTG
PinAI 1177 ACCGGT A^CCGGT
PpuMI 62 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 652 ATTAAT AT^TAAT
RcaI 1476 TCATGA T^CATGA
SacI 1227 GAGCTC GAGCT^C
SexAI 215 ACCTGGT A^CC[AT]GGT
SfaNI 559 GCATC GCATC(5/9)
SfcI 207 CTACAG C^T[GA][CT]AG
SspI 748 AATATT AAT^ATT
TseI 366 GCAGC G^C[AT]GC
XhoII 974 GGATCT [GA]^GATC[CT]
XmnI 352 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 3 GGACC G^G[AT]CC
63 GGACC
BanII 280 GAGCCC G[GA]GC[CT]^C
1227 GAGCTC
BbsI 1464 GAAGAC GAAGAC(2/6)
133 GTCTTC
BfaI 265 CTAG C^TAG
590 CTAG
BmyI 280 GAGCCC G[AGT]GC[ACT]^C
1227 GAGCTC
BpmI 308 CTGGAG CTGGAG(16/14)
1262 CTGGAG
BpuAI 1464 GAAGAC GAAGAC(2/6)
133 GTCTTC
BsmI 460 GAATGC GAATGC(1/-1)
1505 GAATGC
Bsp1286I 280 GAGCCC G[AGT]GC[ACT]^C
1227 GAGCTC
Bsp1407I 182 TGTACA T^GTACA
1326 TGTACA
BsrGI 182 TGTACA T^GTACA
1326 TGTACA
BssKI 216 CCTGG ^CC[ACGT]GG
284 CCTGG
BstNI 216 CCTGG CC^[AT]GG
284 CCTGG
DraII 62 AGGACCT [GA]G^G[ACGT]CC[CT]
1297 AGGGCCT
EcoO109I 62 AGGACCT [GA]G^G[ACGT]CC[CT]
1297 AGGGCCT
EcoRII 216 CCTGG ^CC[AT]GG
284 CCTGG
EcoT14I 99 CCTTGG C^C[AT][AT]GG
347 CCAAGG
MaeI 265 CTAG C^TAG
590 CTAG
MvaI 216 CCTGG CC^[AT]GG
284 CCTGG
ScrFI 216 CCTGG CC^[ACGT]GG
284 CCTGG
SphI 555 GCATGC GCATG^C
1027 GCATGC
SspBI 182 TGTACA T^GTACA
1326 TGTACA
StyI 99 CCTTGG C^C[AT][AT]GG
347 CCAAGG
TaqI 383 TCGA T^CGA
888 TCGA
TthHB8I 383 TCGA T^CGA
888 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaI
BsaJI BsiYI BslI BsmAI BsrI
BstF5I Cac8I Cfr13I DdeI DpnII
EcoRI EcoT22I FokI HaeIII HinfI
HphI MboI MboII MnlI MseI
MslI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I SmlI StuI TfiI Tru9I
Tsp509I TspRI