(Circular) (Six-base) MAPSORT of: pFN21AB7268.seq Check: 2339 from: 1 to: 6349 pFN21AB7268 6349 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2179 2179 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4412 5796 6064 1632 Size: 2780 1384 268 1917 Fragments arranged by size: 2780 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 3529 5248 6225 1203 Size: 2326 1719 977 1327 Fragments arranged by size: 2326 1719 1327 977 AgeI A'CCGG_T Cuts at: 3182 5180 3182 Size: 1998 4351 AhdI GACnn_n'nnGTC Cuts at: 3477 5078 3477 Size: 1601 4748 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2052 1184 Size: 868 5481 AlwNI CAG_nnn'CTG Cuts at: 4092 5664 4092 Size: 1572 4777 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2085 2586 2670 2818 3006 3174 3258 3342 Size: 592 501 84 148 188 168 84 84 Cuts at: 3342 3593 3658 3884 4036 6145 1493 Size: 251 65 226 152 2109 1697 Fragments arranged by size: 2109 1697 592 501 251 226 188 168 152 148 84 84 84 65 AseI AT'TA_AT Cuts at: 160 1032 2658 4903 160 Size: 872 1626 2245 1606 Fragments arranged by size: 2245 1626 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 3674 1870 Size: 73 1731 4545 Fragments arranged by size: 4545 1731 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 3670 5125 618 Size: 325 217 627 380 1503 1455 1842 Fragments arranged by size: 1842 1503 1455 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 2289 3236 3668 1391 Size: 898 947 432 4072 Fragments arranged by size: 4072 947 898 432 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 3476 928 Size: 971 232 1345 3801 Fragments arranged by size: 3801 1345 971 232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 2523 1548 Size: 975 5374 BfrBI ATG'CAT Cuts at: 2564 3032 3514 2564 Size: 468 482 5399 Fragments arranged by size: 5399 482 468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 4410 5566 1790 Size: 2620 1156 2573 Fragments arranged by size: 2620 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2240 4080 5038 Size: 416 53 83 399 24 823 1840 958 Cuts at: 5038 442 Size: 1753 Fragments arranged by size: 1840 1753 958 823 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 3288 5009 1239 Size: 1095 954 1721 2579 Fragments arranged by size: 2579 1721 1095 954 Bpu10I CC'TnA_GC Cuts at: 2014 2014 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2456 3227 3496 4474 5339 5637 2456 Size: 771 269 978 865 298 3168 Fragments arranged by size: 3168 978 865 771 298 269 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2201 2230 3322 5012 882 Size: 617 702 29 1092 1690 2219 Fragments arranged by size: 2219 1690 1092 702 617 29 BsaAI yAC'GTr Cuts at: 493 1204 4140 493 Size: 711 2936 2702 Fragments arranged by size: 2936 2702 711 BsaBI GATnn'nnATC Cuts at: 2525 6343 2525 Size: 3818 2531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3182 4782 5180 5454 5601 6012 3182 Size: 1600 398 274 147 411 3519 Fragments arranged by size: 3519 1600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6124 Fragments arranged by size: 6124 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6349 BseYI C'CCAG_C Cuts at: 5552 6048 5552 Size: 496 5853 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5776 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3236 4410 4495 5566 3236 Size: 1174 85 1071 4019 Fragments arranged by size: 4019 1174 1071 85 BsmI GAATG_Cn' Cuts at: 2471 3516 3860 3953 2471 Size: 1045 344 93 4867 Fragments arranged by size: 4867 1045 344 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2187 2187 Size: 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 3236 3668 4410 4495 5566 1391 Size: 399 499 947 432 742 85 1071 2174 Fragments arranged by size: 2174 1071 947 742 499 432 399 85 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 3481 4238 5968 3481 Size: 757 1730 3862 Fragments arranged by size: 3862 1730 757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 3704 844 Size: 1335 1525 3489 Fragments arranged by size: 3489 1525 1335 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3397 4844 5018 65 Size: 1183 2149 1447 174 1396 Fragments arranged by size: 2149 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3182 4993 5180 1700 Size: 110 1372 1811 187 2869 Fragments arranged by size: 2869 1811 1372 187 110 BsrGI T'GTAC_A Cuts at: 96 2187 3331 96 Size: 2091 1144 3114 Fragments arranged by size: 3114 2091 1144 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 3620 3620 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2979 3679 4046 4431 4448 5900 5986 5998 Size: 1007 700 367 385 17 1452 86 12 Cuts at: 5998 6344 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1007 700 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3865 4659 4679 2165 Size: 1700 794 20 3835 Fragments arranged by size: 3835 1700 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3025 3060 3193 3228 529 Size: 35 2461 35 133 35 3650 Fragments arranged by size: 3650 2461 133 35 35 35 DraI TTT'AAA Cuts at: 2722 3653 2722 Size: 931 5418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 3796 4685 Size: 54 1425 50 275 18 300 1666 889 Cuts at: 4685 6314 8 Size: 1629 43 Fragments arranged by size: 1666 1629 1425 889 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4279 1353 Size: 2926 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3234 3234 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 3288 4412 5009 5796 6064 1239 Size: 393 702 954 1124 597 787 268 1524 Fragments arranged by size: 1524 1124 954 787 702 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2068 3303 2068 Size: 1235 5114 EcoRI G'AATT_C Cuts at: 3006 3174 3258 3342 3658 4036 3006 Size: 168 84 84 316 378 5319 Fragments arranged by size: 5319 378 316 168 84 84 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2246 2278 2899 Size: 32 135 32 485 32 157 32 621 Cuts at: 2899 2931 2971 3003 3067 3099 3403 3435 3463 Size: 32 40 32 64 32 304 32 28 Cuts at: 3463 3495 4990 5022 5064 5096 1373 Size: 32 1495 32 42 32 2626 Fragments arranged by size: 2626 1495 621 485 304 157 135 64 42 40 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3407 3948 3407 Size: 541 5808 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 5463 5647 1305 Size: 879 3279 184 2007 Fragments arranged by size: 3279 2007 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 10 Size: 54 1425 343 300 4227 Fragments arranged by size: 4227 1425 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2379 2398 2779 2847 3015 3183 3351 Size: 648 1213 19 381 68 168 168 168 Cuts at: 3351 3367 3618 4307 4666 4825 518 Size: 16 251 689 359 159 2042 Fragments arranged by size: 2042 1213 689 648 381 359 251 168 168 168 159 68 19 16 MspA1I CmG'CkG Cuts at: 1606 3765 4442 5590 5835 1606 Size: 2159 677 1148 245 2120 Fragments arranged by size: 2159 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 NdeI CA'TA_TG Cuts at: 387 2776 3364 3615 387 Size: 2389 588 251 3121 Fragments arranged by size: 3121 2389 588 251 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NsiI A_TGCA'T Cuts at: 2566 3034 3516 2566 Size: 468 482 5399 Fragments arranged by size: 5399 482 468 NspI r_CATG'y Cuts at: 1132 1229 2378 2564 3036 3533 3707 5252 1132 Size: 97 1149 186 472 497 174 1545 2229 Fragments arranged by size: 2229 1545 1149 497 472 186 174 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 3529 5248 3529 Size: 1719 4630 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2068 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PstI C_TGCA'G Cuts at: 830 3701 830 Size: 2871 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 SacI G_AGCT'C Cuts at: 3236 3236 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SexAI A'CCwGG_T Cuts at: 2220 2220 Size: 6349 SfcI C'TryA_G Cuts at: 826 1045 2212 3697 4832 5513 5704 826 Size: 219 1167 1485 1135 681 191 1471 Fragments arranged by size: 1485 1471 1167 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 3242 3475 4453 5354 5616 Size: 197 926 492 807 233 978 901 262 Cuts at: 5616 820 Size: 1553 Fragments arranged by size: 1553 978 926 901 807 492 262 233 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 2564 3036 3707 1132 Size: 1432 472 671 3774 Fragments arranged by size: 3774 1432 671 472 SspI AAT'ATT Cuts at: 5 52 2755 4273 5 Size: 47 2703 1518 2081 Fragments arranged by size: 2703 2081 1518 47 StuI AGG'CCT Cuts at: 1517 2319 2717 2801 2885 2969 3221 1517 Size: 802 398 84 84 84 252 4645 Fragments arranged by size: 4645 802 398 252 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2104 2352 3788 513 Size: 552 939 100 248 1436 3074 Fragments arranged by size: 3074 1436 939 552 248 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4376 4393 4546 4731 5176 325 Size: 1255 2796 17 153 185 445 1498 Fragments arranged by size: 2796 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2995 3331 Size: 274 80 33 51 1443 210 808 336 Cuts at: 3331 4595 6307 96 Size: 1264 1712 138 Fragments arranged by size: 1712 1443 1264 808 336 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6309 6341 6309 Size: 32 6317 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2361 4478 2361 Size: 2117 4232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI