(Circular) (Six-base) MAPSORT of: pFN21AB3508.seq Check: 1566 from: 1 to: 5689 pFN21AB3508 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2845 2896 2942 3752 5136 5404 1632 Size: 1213 51 46 810 1384 268 1917 Fragments arranged by size: 1917 1384 1213 810 268 51 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2314 4588 5565 1203 Size: 1111 2274 977 1327 Fragments arranged by size: 2274 1327 1111 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2118 2150 2118 Size: 32 5657 AlwNI CAG_nnn'CTG Cuts at: 2519 3432 5004 2519 Size: 913 1572 3204 Fragments arranged by size: 3204 1572 913 ApaI G_GGCC'C Cuts at: 2367 2367 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2074 2998 3224 3376 5485 1493 Size: 581 924 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 581 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2367 2950 3008 1391 Size: 976 583 58 4072 Fragments arranged by size: 4072 976 583 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2317 928 Size: 971 418 4300 Fragments arranged by size: 4300 971 418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2329 3581 4791 2329 Size: 1252 1210 3227 Fragments arranged by size: 3227 1252 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BfrBI ATG'CAT Cuts at: 2645 2797 2645 Size: 152 5537 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2835 5684 2835 Size: 2849 2840 BlpI GC'TnA_GC Cuts at: 2813 3106 2813 Size: 293 5396 Bme1580I G_kGCm'C Cuts at: 1790 2367 3750 4906 1790 Size: 577 1383 1156 2573 Fragments arranged by size: 2573 1383 1156 577 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 2408 4349 1239 Size: 1140 29 1941 2579 Fragments arranged by size: 2579 1941 1140 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2969 3814 4679 4977 2969 Size: 845 865 298 3681 Fragments arranged by size: 3681 865 845 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 2681 4352 882 Size: 617 714 468 1671 2219 Fragments arranged by size: 2219 1671 714 617 468 BsaAI yAC'GTr Cuts at: 493 1204 2317 3480 493 Size: 711 1113 1163 2702 Fragments arranged by size: 2702 1163 1113 711 BsaBI GATnn'nnATC Cuts at: 2723 5683 2723 Size: 2960 2729 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2432 2972 1186 Size: 1219 27 540 3903 Fragments arranged by size: 3903 1219 540 27 BseYI C'CCAG_C Cuts at: 4892 5388 4892 Size: 496 5193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 1163 Size: 573 756 4360 Fragments arranged by size: 4360 756 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2343 2439 3750 3835 4906 2343 Size: 96 1311 85 1071 3126 Fragments arranged by size: 3126 1311 1071 96 85 BsmI GAATG_Cn' Cuts at: 2604 2797 3200 3293 2604 Size: 193 403 93 5000 Fragments arranged by size: 5000 403 193 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 2367 2439 2950 3008 3750 3835 Size: 399 553 24 72 511 58 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 553 511 399 85 72 58 24 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2382 4333 4520 1700 Size: 110 572 1951 187 2869 Fragments arranged by size: 2869 1951 572 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 96 Size: 2202 3487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 2803 794 Size: 2009 3680 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2394 2835 3019 3386 3771 3788 5240 5326 Size: 422 441 184 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 441 422 385 367 346 184 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654 Size: 54 1425 50 275 18 1306 889 1629 Cuts at: 5654 8 Size: 43 Fragments arranged by size: 1629 1425 1306 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 3619 1353 Size: 909 1357 3423 Fragments arranged by size: 3423 1357 909 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2408 2845 2896 2942 3752 4349 Size: 393 747 29 437 51 46 810 597 Cuts at: 4349 5136 5404 1239 Size: 787 268 1524 Fragments arranged by size: 1524 810 787 747 597 437 393 268 51 46 29 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2363 2363 Size: 5689 EcoRI G'AATT_C Cuts at: 2074 2998 3376 2074 Size: 924 378 4387 Fragments arranged by size: 4387 924 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5689 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2601 2633 4330 4362 4404 Size: 32 135 32 1029 32 1697 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1697 1029 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2350 3033 3279 669 Size: 988 281 274 138 683 246 3079 Fragments arranged by size: 3079 988 683 281 274 246 138 HindIII A'AGCT_T Cuts at: 748 2128 2954 748 Size: 1380 826 3483 Fragments arranged by size: 3483 1380 826 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2412 2422 2732 4803 4987 1305 Size: 1107 10 310 2071 184 2007 Fragments arranged by size: 2071 2007 1107 310 184 10 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3647 4006 4165 518 Size: 648 2481 359 159 2042 Fragments arranged by size: 2481 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2889 3105 3782 4930 5175 1606 Size: 1283 216 677 1148 245 2120 Fragments arranged by size: 2120 1283 1148 677 245 216 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NsiI A_TGCA'T Cuts at: 2647 2799 2647 Size: 152 5537 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4411 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 2561 3259 2561 Size: 698 4991 PspOMI G'GGCC_C Cuts at: 2363 2363 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2654 3037 4172 4853 5044 826 Size: 219 1609 383 1135 681 191 1471 Fragments arranged by size: 1609 1471 1135 681 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2984 3793 4694 4956 820 Size: 197 926 1041 809 901 262 1553 Fragments arranged by size: 1553 1041 926 901 809 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2456 2866 1517 Size: 939 410 4340 Fragments arranged by size: 4340 939 410 StyI C'CwwG_G Cuts at: 513 1065 2004 2425 3128 513 Size: 552 939 421 703 3074 Fragments arranged by size: 3074 939 703 552 421 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 3935 5647 Size: 274 80 33 51 1443 321 1637 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1637 1443 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 2272 2304 5649 5681 2070 Size: 32 170 32 3345 32 2078 Fragments arranged by size: 3345 2078 170 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI