################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AccIII AclI AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BanI BbeI BciVI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI KasI KpnI KspI MamI MluI MroI NarI NcoI NdeI NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1941 GACGTC GACGT^C AccII 9 CGCG CG^CG Alw44I 1569 GTGCAC G^TGCAC ApaLI 1569 GTGCAC G^TGCAC BamHI 1797 GGATCC G^GATCC BbrPI 1463 CACGTG CAC^GTG BbsI 172 GAAGAC GAAGAC(2/6) BbvCI 2763 GCTGAGG CCTCAGC(-5/-2) BlpI 69 GCTGAGC GC^T[ACGT]AGC Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC BpuAI 172 GAAGAC GAAGAC(2/6) BsaAI 1463 CACGTG [CT]AC^GT[GA] BsaI 1759 GAGACC GGTCTC(1/5) Bsp1407I 1492 TGTACA T^GTACA BspMI 949 ACCTGC ACCTGC(4/8) BsrGI 1492 TGTACA T^GTACA BstBI 998 TTCGAA TT^CGAA BstUI 9 CGCG CG^CG CelII 69 GCTGAGC GC^T[ACGT]AGC DraI 886 TTTAAA TTT^AAA DraII 856 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 2540 CCGTGG C^C[GA][CT]GG EarI 1712 GAAGAG CTCTTC(1/4) EcoO109I 856 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1444 GAATTC G^AATTC Ksp632I 1712 GAAGAG CTCTTC(1/4) MfeI 305 CAATTG C^AATTG MunI 305 CAATTG C^AATTG MvnI 9 CGCG CG^CG NaeI 1838 GCCGGC GCC^GGC NgoMIV 1838 GCCGGC G^CCGGC NspV 998 TTCGAA TT^CGAA PaeR7I 2027 CTCGAG C^TCGAG PmaCI 1463 CACGTG CAC^GTG PmlI 1463 CACGTG CAC^GTG PpuMI 856 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 2575 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1591 AGTACT AGT^ACT SexAI 2976 ACCTGGT A^CC[AT]GGT SfuI 998 TTCGAA TT^CGAA SspBI 1492 TGTACA T^GTACA SspI 823 AATATT AAT^ATT XbaI 1452 TCTAGA T^CTAGA XhoI 2027 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AflII 94 CTTAAG C^TTAAG 355 CTTAAG AhdI 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1936 GACGTGACGTC AlwNI 1322 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2893 CAGTGGCTG Asp700I 2062 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2268 GAAACAGTTC AspEI 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1936 GACGTGACGTC AvaI 2027 CTCGAG C^[CT]CG[GA]G 2347 CCCGAG BalI 1688 TGGCCA TGG^CCA 1895 TGGCCA BcgI 1996 CGAGACGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2115 CGATGCTCCTGC BclI 1865 TGATCA T^GATCA 2480 TGATCA BfrI 94 CTTAAG C^TTAAG 355 CTTAAG BglI 41 GCCACTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1907 GCCGCATCGGC BpmI 677 CTGGAG CTGGAG(16/14) 1613 CTGGAG BsmBI 1997 GAGACG CGTCTC(1/5) 2941 GAGACG BsmFI 1874 GGGAC GGGAC(10/14) 580 GTCCC BsoBI 2027 CTCGAG C^[CT]CG[GA]G 2347 CCCGAG BsrFI 123 ACCGGC [GA]^CCGG[CT] 1838 GCCGGC BssSI 590 CACGAG CACGAG(-5/-1) 2794 CTCGTG BstYI 701 AGATCC [GA]^GATC[CT] 1797 GGATCC Cfr10I 123 ACCGGC [GA]^CCGG[CT] 1838 GCCGGC Eam1105I 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1936 GACGTGACGTC EcoNI 75 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2759 CCTTGCTGAGG EcoT14I 1023 CCTTGG C^C[AT][AT]GG 2337 CCTTGG FbaI 1865 TGATCA T^GATCA 2480 TGATCA HaeII 2530 GGCGCT [GA]GCGC^[CT] 2694 GGCGCT HindIII 91 AAGCTT A^AGCTT 1949 AAGCTT HpaI 492 GTTAAC GTT^AAC 682 GTTAAC MflI 701 AGATCC [GA]^GATC[CT] 1797 GGATCC MluNI 1688 TGGCCA TGG^CCA 1895 TGGCCA MscI 1688 TGGCCA TGG^CCA 1895 TGGCCA PstI 935 CTGCAG CTGCA^G 1089 CTGCAG SphI 1790 GCATGC GCATG^C 3025 GCATGC StyI 1023 CCTTGG C^C[AT][AT]GG 2337 CCTTGG XcmI 299 CCAATGCAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1575 CCACATTCGAGTTGG XhoII 701 AGATCC [GA]^GATC[CT] 1797 GGATCC XmnI 2062 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2268 GAAACAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I