(Circular) (Six-base) MAPSORT of: pFN21AB3246.seq Check: 7908 from: 1 to: 7831 pFN21AB3246 7831 bp With 182 enzymes: SgfI * February 11, 2011 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2963 2963 Size: 7831 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3950 5641 278 Size: 53 83 186 936 2414 1691 2468 Fragments arranged by size: 2468 2414 1691 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5174 1937 Size: 3237 4594 Acc65I G'GTAC_C Cuts at: 5152 5152 Size: 7831 AclI AA'CG_TT Cuts at: 1563 5958 6331 1563 Size: 4395 373 3063 Fragments arranged by size: 4395 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2735 2949 3576 4058 4154 5894 7278 Size: 522 581 214 627 482 96 1740 1384 Cuts at: 7278 7546 1632 Size: 268 1917 Fragments arranged by size: 1917 1740 1384 627 581 522 482 268 214 96 AflII C'TTAA_G Cuts at: 820 1017 2099 2360 820 Size: 197 1082 261 6291 Fragments arranged by size: 6291 1082 261 197 AflIII A'CryG_T Cuts at: 1203 6730 7707 1203 Size: 5527 977 1327 Fragments arranged by size: 5527 1327 977 AgeI A'CCGG_T Cuts at: 6662 6662 Size: 7831 AhdI GACnn_n'nnGTC Cuts at: 3478 3946 6560 3478 Size: 468 2614 4749 Fragments arranged by size: 4749 2614 468 AleI CACnn'nnGTG Cuts at: 1166 3896 4175 4229 1166 Size: 2730 279 54 4768 Fragments arranged by size: 4768 2730 279 54 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3430 1184 Size: 2246 5585 AlwNI CAG_nnn'CTG Cuts at: 3332 4903 5574 7146 3332 Size: 1571 671 1572 4017 Fragments arranged by size: 4017 1572 1571 671 ApaLI G'TGCA_C Cuts at: 3574 5888 7044 3574 Size: 2314 1156 4361 Fragments arranged by size: 4361 2314 1156 ApoI r'AATT_y Cuts at: 1493 2768 3449 4067 5140 5366 5518 7627 1493 Size: 1275 681 618 1073 226 152 2109 1697 Fragments arranged by size: 2109 1697 1275 1073 681 618 226 152 AseI AT'TA_AT Cuts at: 160 1032 6385 160 Size: 872 5353 1606 Fragments arranged by size: 5353 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 AvaI C'yCGr_G Cuts at: 1870 1943 4032 4352 5156 1870 Size: 73 2089 320 804 4545 Fragments arranged by size: 4545 2089 804 320 73 BamHI G'GATC_C Cuts at: 3802 5161 5528 3802 Size: 1359 367 6105 Fragments arranged by size: 6105 1359 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5152 6607 618 Size: 325 217 627 3365 1455 1842 Fragments arranged by size: 3365 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2117 4166 4808 5150 1391 Size: 726 2049 642 342 4072 Fragments arranged by size: 4072 2049 726 642 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 928 Size: 971 285 6575 Fragments arranged by size: 6575 971 285 BbvCI CC'TCA_GC Cuts at: 4769 4769 Size: 7831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3990 4024 4109 4143 6022 6056 1903 Size: 34 2053 34 85 34 1879 34 3678 Fragments arranged by size: 3678 2053 1879 85 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5723 6933 5723 Size: 1210 6621 BclI T'GATC_A Cuts at: 1548 3870 4485 1548 Size: 2322 615 4894 Fragments arranged by size: 4894 2322 615 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 3918 6442 Size: 107 122 71 631 605 380 1866 2524 Cuts at: 6442 136 Size: 1525 Fragments arranged by size: 2524 1866 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 7826 7826 Size: 7831 BlpI GC'TnA_GC Cuts at: 2075 5248 2075 Size: 3173 4658 Bme1580I G_kGCm'C Cuts at: 1790 2838 3578 3893 5892 7048 1790 Size: 1048 740 315 1999 1156 2573 Fragments arranged by size: 2573 1999 1156 1048 740 315 BmgBI CAC'GTC Cuts at: 1318 3943 1318 Size: 2625 5206 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4430 4891 Size: 416 53 83 399 24 2468 545 461 Cuts at: 4891 5562 6520 442 Size: 671 958 1753 Fragments arranged by size: 2468 1753 958 671 545 461 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7831 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3841 3841 Size: 7831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2703 3639 6491 1239 Size: 1464 936 2852 2579 Fragments arranged by size: 2852 2579 1464 936 Bpu10I CC'TnA_GC Cuts at: 4769 4769 Size: 7831 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3755 4937 5956 6821 7119 3755 Size: 1182 1019 865 298 4467 Fragments arranged by size: 4467 1182 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3758 6494 882 Size: 617 2259 2736 2219 Fragments arranged by size: 2736 2259 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3470 5622 493 Size: 711 2266 2152 2702 Fragments arranged by size: 2702 2266 2152 711 BsaBI GATnn'nnATC Cuts at: 7825 7825 Size: 7831 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3947 4005 5638 Size: 53 83 186 936 522 1892 58 1633 Cuts at: 5638 6020 6655 275 Size: 382 635 1451 Fragments arranged by size: 1892 1633 1451 936 635 522 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 6264 6662 6936 7083 7494 6264 Size: 398 274 147 411 6601 Fragments arranged by size: 6601 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2658 2688 4205 4235 1178 Size: 30 165 30 1255 30 1517 30 4774 Fragments arranged by size: 4774 1517 1255 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 3817 4259 1186 Size: 862 1769 442 4758 Fragments arranged by size: 4758 1769 862 442 BseYI C'CCAG_C Cuts at: 2041 2651 3996 7034 7530 2041 Size: 610 1345 3038 496 2342 Fragments arranged by size: 3038 2342 1345 610 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 4401 5018 1163 Size: 573 699 1966 617 3976 Fragments arranged by size: 3976 1966 699 617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5281 6042 7070 1540 Size: 275 186 3280 761 1028 2301 Fragments arranged by size: 3280 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3259 3553 3578 5892 5977 7048 3259 Size: 294 25 2314 85 1071 4042 Fragments arranged by size: 4042 2314 1071 294 85 25 BsmI GAATG_Cn' Cuts at: 5342 5435 5342 Size: 93 7738 BsmBI CGTCTCn'nnnn_ Cuts at: 3996 4940 3996 Size: 944 6887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2838 3259 3553 3578 3893 4166 Size: 399 327 721 421 294 25 315 273 Cuts at: 4166 4808 5150 5892 5977 7048 1391 Size: 642 342 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 721 642 421 399 342 327 315 294 273 85 25 BspEI T'CCGG_A Cuts at: 7494 7494 Size: 7831 BspHI T'CATG_A Cuts at: 3700 3867 4449 5720 7450 3700 Size: 167 582 1271 1730 4081 Fragments arranged by size: 4081 1730 1271 582 167 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 5186 844 Size: 2119 2223 3489 Fragments arranged by size: 3489 2223 2119 BsrBI CCG'CTC Cuts at: 1394 1823 5718 1394 Size: 429 3895 3507 Fragments arranged by size: 3895 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2808 4152 4569 6326 6500 65 Size: 1183 1395 165 1344 417 1757 174 1396 Fragments arranged by size: 1757 1396 1395 1344 1183 417 174 165 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 3843 6475 6662 1700 Size: 110 318 1715 2632 187 2869 Fragments arranged by size: 2869 2632 1715 318 187 110 BsrGI T'GTAC_A Cuts at: 96 3497 96 Size: 3401 4430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7831 BssSI C'ACGA_G Cuts at: 2595 4799 5891 6903 2595 Size: 2204 1092 1012 3523 Fragments arranged by size: 3523 2204 1092 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7831 BstBI TT'CG_AA Cuts at: 3004 6635 3004 Size: 3631 4200 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7831 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2959 3227 4231 4420 2029 Size: 930 268 1004 189 5440 Fragments arranged by size: 5440 1004 930 268 189 BstYI r'GATC_y Cuts at: 1972 2706 3802 5161 5528 5913 5930 7382 7468 Size: 734 1096 1359 367 385 17 1452 86 Cuts at: 7468 7480 7826 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1359 1096 734 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4545 513 Size: 552 939 2541 3799 Fragments arranged by size: 3799 2541 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3694 4122 487 Size: 683 56 2468 428 4196 Fragments arranged by size: 4196 2468 683 428 56 BtsI GCAGTG_nn' Cuts at: 2907 3674 3891 4170 4413 5347 6141 6161 2907 Size: 767 217 279 243 934 794 20 4577 Fragments arranged by size: 4577 934 794 767 279 243 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7796 DraI TTT'AAA Cuts at: 2893 5135 2893 Size: 2242 5589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6838 809 Size: 507 5522 1802 Fragments arranged by size: 5522 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3693 3900 5051 Size: 54 1425 50 275 18 1863 207 1151 Cuts at: 5051 5278 6167 7796 8 Size: 227 889 1629 43 Fragments arranged by size: 1863 1629 1425 1151 889 275 227 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5278 1537 Size: 275 3466 4090 Fragments arranged by size: 4090 3466 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 5761 1353 Size: 2359 2049 3423 Fragments arranged by size: 3423 2359 2049 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3982 4223 6418 6792 6938 387 Size: 1064 138 2393 241 2195 374 146 1280 Fragments arranged by size: 2393 2195 1280 1064 374 241 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2703 2735 2949 3576 3639 4058 Size: 393 522 549 32 214 627 63 419 Cuts at: 4058 4154 5894 6491 7278 7546 1239 Size: 96 1740 597 787 268 1524 Fragments arranged by size: 1740 1524 787 627 597 549 522 419 393 268 214 96 63 32 EcoNI CCTnn'n_nnAGG Cuts at: 2084 4768 2084 Size: 2684 5147 EcoO109I rG'GnC_Cy Cuts at: 2862 2862 Size: 7831 EcoRI G'AATT_C Cuts at: 3449 5140 5518 3449 Size: 1691 378 5762 Fragments arranged by size: 5762 1691 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2517 2517 Size: 7831 FspI TGC'GCA Cuts at: 6337 6337 Size: 7831 HaeII r_GCGC'y Cuts at: 1678 4539 4703 6978 1678 Size: 2861 164 2275 2531 Fragments arranged by size: 2861 2531 2275 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3464 3496 3932 3964 4205 Size: 32 135 32 1892 32 436 32 241 Cuts at: 4205 4237 6472 6504 6546 6578 1373 Size: 32 2235 32 42 32 2626 Fragments arranged by size: 2626 2235 1892 436 241 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2059 2499 2530 2605 2689 5175 Size: 988 281 121 440 31 75 84 2486 Cuts at: 5175 5421 669 Size: 246 3079 Fragments arranged by size: 3079 2486 988 440 281 246 121 84 75 31 HindIII A'AGCT_T Cuts at: 748 2096 3954 748 Size: 1348 1858 4625 Fragments arranged by size: 4625 1858 1348 HpaI GTT'AAC Cuts at: 2499 2689 5421 2499 Size: 190 2732 4909 Fragments arranged by size: 4909 2732 190 KpnI G_GTAC'C Cuts at: 5156 5156 Size: 7831 MfeI C'AATT_G Cuts at: 2310 5430 2310 Size: 3120 4711 MluI A'CGCG_T Cuts at: 7707 7707 Size: 7831 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 3295 3571 4326 6945 7129 1305 Size: 837 1153 276 755 2619 184 2007 Fragments arranged by size: 2619 2007 1153 837 755 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3695 3902 10 Size: 54 1425 343 1863 207 3939 Fragments arranged by size: 3939 1863 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2841 3195 3222 3679 3896 4157 4175 Size: 648 1675 354 27 457 217 261 18 Cuts at: 4175 4229 5789 6148 6307 518 Size: 54 1560 359 159 2042 Fragments arranged by size: 2042 1675 1560 648 457 359 354 261 217 159 54 27 18 MspA1I CmG'CkG Cuts at: 1606 2419 3436 4023 4375 4491 4609 4885 5247 Size: 813 1017 587 352 116 118 276 362 Cuts at: 5247 5924 7072 7317 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 813 677 587 362 352 276 245 118 116 NaeI GCC'GGC Cuts at: 3845 3845 Size: 7831 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6340 Fragments arranged by size: 6340 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7831 NgoMIV G'CCGG_C Cuts at: 3843 3843 Size: 7831 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7831 NotI GC'GGCC_GC Cuts at: 5278 5278 Size: 7831 NspI r_CATG'y Cuts at: 1132 1229 2214 2626 3799 5034 5189 6734 1132 Size: 97 985 412 1173 1235 155 1545 2229 Fragments arranged by size: 2229 1545 1235 1173 985 412 155 97 PasI CC'CwG_GG Cuts at: 1348 4782 1348 Size: 3434 4397 PciI A'CATG_T Cuts at: 6730 6730 Size: 7831 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7831 PfoI T'CCnGG_A Cuts at: 2709 2864 2709 Size: 155 7676 PmeI GTTT'AAAC Cuts at: 5135 5135 Size: 7831 PmlI CAC'GTG Cuts at: 3470 3470 Size: 7831 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5900 5932 7413 7445 5900 Size: 32 1481 32 6286 Fragments arranged by size: 6286 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2862 2862 Size: 7831 PshAI GACnn'nnGTC Cuts at: 1613 4584 1613 Size: 2971 4860 PsiI TTA'TAA Cuts at: 5401 5401 Size: 7831 PspXI vC'TCGA_Gb Cuts at: 4032 4032 Size: 7831 PstI C_TGCA'G Cuts at: 830 2944 3098 5183 830 Size: 2114 154 2085 3478 Fragments arranged by size: 3478 2114 2085 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7831 PvuII CAG'CTG Cuts at: 3436 4375 4609 3436 Size: 939 234 6658 Fragments arranged by size: 6658 939 234 SalI G'TCGA_C Cuts at: 1936 5173 1936 Size: 3237 4594 SbfI CC_TGCA'GG Cuts at: 5183 5183 Size: 7831 ScaI AGT'ACT Cuts at: 3598 6079 7791 3598 Size: 2481 1712 3638 Fragments arranged by size: 3638 2481 1712 SexAI A'CCwGG_T Cuts at: 4981 4981 Size: 7831 SfcI C'TryA_G Cuts at: 826 1045 2913 2940 3094 3324 4622 4719 5092 Size: 219 1868 27 154 230 1298 97 373 Cuts at: 5092 5179 6314 6995 7186 826 Size: 87 1135 681 191 1471 Fragments arranged by size: 1868 1471 1298 1135 681 373 230 219 191 154 97 87 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7831 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7831 SmaI CCC'GGG Cuts at: 1872 5158 1872 Size: 3286 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 2360 3734 4032 4952 5935 Size: 197 926 156 261 1374 298 920 983 Cuts at: 5935 6836 7098 820 Size: 901 262 1553 Fragments arranged by size: 1553 1374 983 926 920 901 298 262 261 197 156 SnaBI TAC'GTA Cuts at: 493 5622 493 Size: 5129 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7831 SphI G_CATG'C Cuts at: 1132 3799 5034 5189 1132 Size: 2667 1235 155 3774 Fragments arranged by size: 3774 2667 1235 155 SspI AAT'ATT Cuts at: 5 52 2830 5755 5 Size: 47 2778 2925 2081 Fragments arranged by size: 2925 2778 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7831 StyI C'CwwG_G Cuts at: 513 1065 2004 3028 4342 5270 513 Size: 552 939 1024 1314 928 3074 Fragments arranged by size: 3074 1314 1024 939 928 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 3593 4538 5858 5875 6028 6213 Size: 1255 726 1287 945 1320 17 153 185 Cuts at: 6213 6658 325 Size: 445 1498 Fragments arranged by size: 1498 1320 1287 1255 945 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3035 3497 3596 Size: 274 80 33 51 1443 1058 462 99 Cuts at: 3596 6077 7789 96 Size: 2481 1712 138 Fragments arranged by size: 2481 1712 1443 1058 462 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2860 2892 4818 4850 4854 4886 7791 7823 2860 Size: 32 1926 32 4 32 2905 32 2868 Fragments arranged by size: 2905 2868 1926 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7831 XbaI T'CTAG_A Cuts at: 3457 5167 3457 Size: 1710 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 3587 2311 Size: 1276 6555 XhoI C'TCGA_G Cuts at: 1943 4032 1943 Size: 2089 5742 XmaI C'CCGG_G Cuts at: 1870 5156 1870 Size: 3286 4545 XmnI GAAnn'nnTTC Cuts at: 4071 4277 5960 4071 Size: 206 1683 5942 Fragments arranged by size: 5942 1683 206 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3948 5639 276 Size: 53 83 186 936 2414 1691 2468 Fragments arranged by size: 2468 2414 1691 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI