(Linear) (Six-base) MAPSORT of: KIBB3246.seq Check: 528 from: 1 to: 3126 KIBB3246.orf With 182 enzymes: SgfI * February 11, 2011 17:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 958 3126 Size: 958 2168 AatII G_ACGT'C Cuts at: 0 1945 3126 Size: 1945 1181 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 730 944 1571 2053 2149 3126 Size: 149 581 214 627 482 96 977 Fragments arranged by size: 977 627 581 482 214 149 96 AflII C'TTAA_G Cuts at: 0 94 355 3126 Size: 94 261 2771 Fragments arranged by size: 2771 261 94 AhdI GACnn_n'nnGTC Cuts at: 0 1473 1941 3126 Size: 1473 468 1185 Fragments arranged by size: 1473 1185 468 AleI CACnn'nnGTG Cuts at: 0 1891 2170 2224 3126 Size: 1891 279 54 902 Fragments arranged by size: 1891 902 279 54 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1425 3126 Size: 1425 1701 AlwNI CAG_nnn'CTG Cuts at: 0 1327 2898 3126 Size: 1327 1571 228 Fragments arranged by size: 1571 1327 228 ApaLI G'TGCA_C Cuts at: 0 1569 3126 Size: 1569 1557 ApoI r'AATT_y Cuts at: 0 763 1444 2062 3126 Size: 763 681 618 1064 Fragments arranged by size: 1064 763 681 618 AvaI C'yCGr_G Cuts at: 0 2027 2347 3126 Size: 2027 320 779 Fragments arranged by size: 2027 779 320 BamHI G'GATC_C Cuts at: 0 1797 3126 Size: 1797 1329 BanII G_rGCy'C Cuts at: 0 112 2161 2803 3126 Size: 112 2049 642 323 Fragments arranged by size: 2049 642 323 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 179 3126 Size: 179 2947 BbvCI CC'TCA_GC Cuts at: 0 2764 3126 Size: 2764 362 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1985 2019 2104 2138 3126 Size: 1985 34 85 34 988 Fragments arranged by size: 1985 988 85 34 34 BclI T'GATC_A Cuts at: 0 1865 2480 3126 Size: 1865 615 646 Fragments arranged by size: 1865 646 615 BglI GCCn_nnn'nGGC Cuts at: 0 47 1913 3126 Size: 47 1866 1213 Fragments arranged by size: 1866 1213 47 BlpI GC'TnA_GC Cuts at: 0 70 3126 Size: 70 3056 Bme1580I G_kGCm'C Cuts at: 0 833 1573 1888 3126 Size: 833 740 315 1238 Fragments arranged by size: 1238 833 740 315 BmgBI CAC'GTC Cuts at: 0 1938 3126 Size: 1938 1188 BmrI ACTGGGnnnn_n' Cuts at: 0 1880 2425 2886 3126 Size: 1880 545 461 240 Fragments arranged by size: 1880 545 461 240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1836 3126 Size: 1836 1290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 698 1634 3126 Size: 698 936 1492 Fragments arranged by size: 1492 936 698 Bpu10I CC'TnA_GC Cuts at: 0 2764 3126 Size: 2764 362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1750 2932 3126 Size: 1750 1182 194 Fragments arranged by size: 1750 1182 194 BsaI GGTCTCn'nnnn_ Cuts at: 0 1753 3126 Size: 1753 1373 BsaAI yAC'GTr Cuts at: 0 1465 3126 Size: 1465 1661 BsaHI Gr'CG_yC Cuts at: 0 50 1942 2000 3126 Size: 50 1892 58 1126 Fragments arranged by size: 1892 1126 58 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 653 683 2200 2230 3126 Size: 653 30 1517 30 896 Fragments arranged by size: 1517 896 653 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 1812 2254 3126 Size: 43 1769 442 872 Fragments arranged by size: 1769 872 442 43 BseYI C'CCAG_C Cuts at: 0 36 646 1991 3126 Size: 36 610 1345 1135 Fragments arranged by size: 1345 1135 610 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 2396 3013 3126 Size: 430 1966 617 113 Fragments arranged by size: 1966 617 430 113 BsiHKAI G_wGCw'C Cuts at: 0 1254 1548 1573 3126 Size: 1254 294 25 1553 Fragments arranged by size: 1553 1254 294 25 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1991 2935 3126 Size: 1991 944 191 Fragments arranged by size: 1991 944 191 Bsp1286I G_dGCh'C Cuts at: 0 112 833 1254 1548 1573 1888 2161 2803 Size: 112 721 421 294 25 315 273 642 Cuts at: 2803 3126 Size: 323 Fragments arranged by size: 721 642 421 323 315 294 273 112 25 BspHI T'CATG_A Cuts at: 0 1695 1862 2444 3126 Size: 1695 167 582 682 Fragments arranged by size: 1695 682 582 167 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 958 3126 Size: 958 2168 BsrDI GCAATG_nn' Cuts at: 0 638 803 2147 2564 3126 Size: 638 165 1344 417 562 Fragments arranged by size: 1344 638 562 417 165 BsrFI r'CCGG_y Cuts at: 0 123 1838 3126 Size: 123 1715 1288 Fragments arranged by size: 1715 1288 123 BsrGI T'GTAC_A Cuts at: 0 1492 3126 Size: 1492 1634 BssSI C'ACGA_G Cuts at: 0 590 2794 3126 Size: 590 2204 332 Fragments arranged by size: 2204 590 332 BstBI TT'CG_AA Cuts at: 0 999 3126 Size: 999 2127 BstXI CCAn_nnnn'nTGG Cuts at: 0 24 954 1222 2226 2415 3126 Size: 24 930 268 1004 189 711 Fragments arranged by size: 1004 930 711 268 189 24 BstYI r'GATC_y Cuts at: 0 701 1797 3126 Size: 701 1096 1329 Fragments arranged by size: 1329 1096 701 BtgI C'CryG_G Cuts at: 0 2540 3126 Size: 2540 586 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1689 2117 3126 Size: 1689 428 1009 Fragments arranged by size: 1689 1009 428 BtsI GCAGTG_nn' Cuts at: 0 902 1669 1886 2165 2408 3126 Size: 902 767 217 279 243 718 Fragments arranged by size: 902 767 718 279 243 217 DraI TTT'AAA Cuts at: 0 888 3126 Size: 888 2238 EaeI y'GGCC_r Cuts at: 0 1688 1895 3046 3126 Size: 1688 207 1151 80 Fragments arranged by size: 1688 1151 207 80 EarI CTCTTCn'nnn_ Cuts at: 0 1707 3126 Size: 1707 1419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1977 2218 3126 Size: 1977 241 908 Fragments arranged by size: 1977 908 241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 698 730 944 1571 1634 2053 2149 Size: 149 549 32 214 627 63 419 96 Cuts at: 2149 3126 Size: 977 Fragments arranged by size: 977 627 549 419 214 149 96 63 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 79 2763 3126 Size: 79 2684 363 Fragments arranged by size: 2684 363 79 EcoO109I rG'GnC_Cy Cuts at: 0 857 3126 Size: 857 2269 EcoRI G'AATT_C Cuts at: 0 1444 3126 Size: 1444 1682 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 512 3126 Size: 512 2614 HaeII r_GCGC'y Cuts at: 0 2534 2698 3126 Size: 2534 164 428 Fragments arranged by size: 2534 428 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1459 1491 1927 1959 2200 2232 3126 Size: 1459 32 436 32 241 32 894 Fragments arranged by size: 1459 894 436 241 32 32 32 HincII GTy'rAC Cuts at: 0 54 494 525 600 684 3126 Size: 54 440 31 75 84 2442 Fragments arranged by size: 2442 440 84 75 54 31 HindIII A'AGCT_T Cuts at: 0 91 1949 3126 Size: 91 1858 1177 Fragments arranged by size: 1858 1177 91 HpaI GTT'AAC Cuts at: 0 494 684 3126 Size: 494 190 2442 Fragments arranged by size: 2442 494 190 MfeI C'AATT_G Cuts at: 0 305 3126 Size: 305 2821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 137 1290 1566 2321 3126 Size: 137 1153 276 755 805 Fragments arranged by size: 1153 805 755 276 137 MscI TGG'CCA Cuts at: 0 1690 1897 3126 Size: 1690 207 1229 Fragments arranged by size: 1690 1229 207 MslI CAynn'nnrTG Cuts at: 0 836 1190 1217 1674 1891 2152 2170 2224 Size: 836 354 27 457 217 261 18 54 Cuts at: 2224 3126 Size: 902 Fragments arranged by size: 902 836 457 354 261 217 54 27 18 MspA1I CmG'CkG Cuts at: 0 414 1431 2018 2370 2486 2604 2880 3126 Size: 414 1017 587 352 116 118 276 246 Fragments arranged by size: 1017 587 414 352 276 246 118 116 NaeI GCC'GGC Cuts at: 0 1840 3126 Size: 1840 1286 NgoMIV G'CCGG_C Cuts at: 0 1838 3126 Size: 1838 1288 NspI r_CATG'y Cuts at: 0 209 621 1794 3029 3126 Size: 209 412 1173 1235 97 Fragments arranged by size: 1235 1173 412 209 97 PasI CC'CwG_GG Cuts at: 0 2777 3126 Size: 2777 349 PfoI T'CCnGG_A Cuts at: 0 704 859 3126 Size: 704 155 2267 Fragments arranged by size: 2267 704 155 PmlI CAC'GTG Cuts at: 0 1465 3126 Size: 1465 1661 PpuMI rG'GwC_Cy Cuts at: 0 857 3126 Size: 857 2269 PshAI GACnn'nnGTC Cuts at: 0 2579 3126 Size: 2579 547 PspXI vC'TCGA_Gb Cuts at: 0 2027 3126 Size: 2027 1099 PstI C_TGCA'G Cuts at: 0 939 1093 3126 Size: 939 154 2033 Fragments arranged by size: 2033 939 154 PvuII CAG'CTG Cuts at: 0 1431 2370 2604 3126 Size: 1431 939 234 522 Fragments arranged by size: 1431 939 522 234 ScaI AGT'ACT Cuts at: 0 1593 3126 Size: 1593 1533 SexAI A'CCwGG_T Cuts at: 0 2976 3126 Size: 2976 150 SfcI C'TryA_G Cuts at: 0 908 935 1089 1319 2617 2714 3087 3126 Size: 908 27 154 230 1298 97 373 39 Fragments arranged by size: 1298 908 373 230 154 97 39 27 SmlI C'TyrA_G Cuts at: 0 94 355 1729 2027 2947 3126 Size: 94 261 1374 298 920 179 Fragments arranged by size: 1374 920 298 261 179 94 SphI G_CATG'C Cuts at: 0 1794 3029 3126 Size: 1794 1235 97 Fragments arranged by size: 1794 1235 97 SspI AAT'ATT Cuts at: 0 825 3126 Size: 825 2301 StyI C'CwwG_G Cuts at: 0 1023 2337 3126 Size: 1023 1314 789 Fragments arranged by size: 1314 1023 789 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 301 1588 2533 3126 Size: 301 1287 945 593 Fragments arranged by size: 1287 945 593 301 TatI w'GTAC_w Cuts at: 0 1030 1492 1591 3126 Size: 1030 462 99 1535 Fragments arranged by size: 1535 1030 462 99 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 855 887 2813 2845 2849 2881 3126 Size: 855 32 1926 32 4 32 245 Fragments arranged by size: 1926 855 245 32 32 32 4 XbaI T'CTAG_A Cuts at: 0 1452 3126 Size: 1452 1674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 1582 3126 Size: 306 1276 1544 Fragments arranged by size: 1544 1276 306 XhoI C'TCGA_G Cuts at: 0 2027 3126 Size: 2027 1099 XmnI GAAnn'nnTTC Cuts at: 0 2066 2272 3126 Size: 2066 206 854 Fragments arranged by size: 2066 854 206 ZraI GAC'GTC Cuts at: 0 1943 3126 Size: 1943 1183 Enzymes that do cut: AarI AatII AcuI AflII AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI PmlI PpuMI PshAI PspXI PstI PvuII ScaI SexAI SfcI SmlI SphI SspI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflIII AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BciVI BfrBI BglII BmtI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI KasI KpnI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XmaI