Restriction map for
pFN21AB9774
(Circular) (Six-base) MAPSORT of: pFN21AB9774.seq Check: 2383 from: 1 to: 6718
pFN21AB9774 6718 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3777 3957 3777
Size: 180 6538
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4528 278
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 4061 1937
Size: 670 1454 4594
Fragments arranged by size:
4594 1454 670
Acc65I G'GTAC_C
Cuts at: 4039 4039
Size: 6718
AclI AA'CG_TT
Cuts at: 1563 4845 5218 1563
Size: 3282 373 3063
Fragments arranged by size:
3282 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4781 6165 6433 1632
Size: 3149 1384 268 1917
Fragments arranged by size:
3149 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6521
AflIII A'CryG_T
Cuts at: 1203 3894 5617 6594 1203
Size: 2691 1723 977 1327
Fragments arranged by size:
2691 1723 1327 977
AgeI A'CCGG_T
Cuts at: 5549 5549
Size: 6718
AhdI GACnn_n'nnGTC
Cuts at: 2983 3097 5447 2983
Size: 114 2350 4254
Fragments arranged by size:
4254 2350 114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6718
AlwNI CAG_nnn'CTG
Cuts at: 2041 2056 2095 2362 3292 3367 3501 3787 3853
Size: 15 39 267 930 75 134 286 66
Cuts at: 3853 4461 6033 2041
Size: 608 1572 2726
Fragments arranged by size:
2726 1572 930 608 286 267 134 75
66 39 15
ApaI G_GGCC'C
Cuts at: 2089 3977 2089
Size: 1888 4830
ApaLI G'TGCA_C
Cuts at: 3816 3956 4775 5931 3816
Size: 140 819 1156 4603
Fragments arranged by size:
4603 1156 819 140
ApoI r'AATT_y
Cuts at: 1493 2429 4027 4253 4405 6514 1493
Size: 936 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 936 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5272 160
Size: 872 4240 1606
Fragments arranged by size:
4240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
AvaI C'yCGr_G
Cuts at: 1870 1943 2563 3319 3331 3371 3566 3679 3970
Size: 73 620 756 12 40 195 113 291
Cuts at: 3970 4043 1870
Size: 73 4545
Fragments arranged by size:
4545 756 620 291 195 113 73 73
40 12
BamHI G'GATC_C
Cuts at: 2824 3058 4048 4415 2824
Size: 234 990 367 5127
Fragments arranged by size:
5127 990 367 234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2851 3394 3539 3980
Size: 325 217 627 449 615 543 145 441
Cuts at: 3980 4039 5494 618
Size: 59 1455 1842
Fragments arranged by size:
1842 1455 627 615 543 449 441 325
217 145 59
BanII G_rGCy'C
Cuts at: 1391 2089 2271 2729 2766 2847 3977 4037 1391
Size: 698 182 458 37 81 1130 60 4072
Fragments arranged by size:
4072 1130 698 458 182 81 60 37
BbeI G_GCGC'C
Cuts at: 3398 3543 3984 3398
Size: 145 441 6132
Fragments arranged by size:
6132 441 145
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2236 2637 3752 928
Size: 971 337 401 1115 3894
Fragments arranged by size:
3894 1115 971 401 337
BbvCI CC'TCA_GC
Cuts at: 2034 2034
Size: 6718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3565 3599 4909 4943 1903
Size: 34 50 34 1544 34 1310 34 3678
Fragments arranged by size:
3678 1544 1310 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4610 5820 4610
Size: 1210 5508
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6718
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 3681 5329
Size: 107 122 71 631 605 649 1360 1648
Cuts at: 5329 136
Size: 1525
Fragments arranged by size:
1648 1525 1360 649 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2289 6713 2289
Size: 4424 2294
BlpI GC'TnA_GC
Cuts at: 2760 4135 2760
Size: 1375 5343
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2239 3566 3820 3960 3977 4779 5935
Size: 299 150 1327 254 140 17 802 1156
Cuts at: 5935 1790
Size: 2573
Fragments arranged by size:
2573 1327 1156 802 299 254 150 140
17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6718
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 4449 5407
Size: 416 53 83 399 24 849 2183 958
Cuts at: 5407 442
Size: 1753
Fragments arranged by size:
2183 1753 958 849 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6718
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2390 2528 2733 2942 5378 1239
Size: 859 292 138 205 209 2436 2579
Fragments arranged by size:
2579 2436 859 292 209 205 138
Bpu10I CC'TnA_GC
Cuts at: 2034 4008 2034
Size: 1974 4744
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2157 3842 3923 4843 5708 6006 2157
Size: 1685 81 920 865 298 2869
Fragments arranged by size:
2869 1685 920 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5381 882
Size: 617 3882 2219
Fragments arranged by size:
3882 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 4509 493
Size: 711 2693 612 2702
Fragments arranged by size:
2702 2693 711 612
BsaBI GATnn'nnATC
Cuts at: 6712 6712
Size: 6718
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3395 3540 3981
Size: 53 83 186 936 657 1205 145 441
Cuts at: 3981 4525 4907 5542 275
Size: 544 382 635 1451
Fragments arranged by size:
1451 1205 936 657 635 544 441 382
186 145 83 53
BsaWI w'CCGG_w
Cuts at: 5151 5549 5823 5970 6381 5151
Size: 398 274 147 411 5488
Fragments arranged by size:
5488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 2996 3026 3978
Size: 30 165 30 1035 30 528 30 952
Cuts at: 3978 4008 1178
Size: 30 3888
Fragments arranged by size:
3888 1035 952 528 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 3017 3131 3518 1186
Size: 934 897 114 387 4386
Fragments arranged by size:
4386 934 897 387 114
BseYI C'CCAG_C
Cuts at: 2041 2082 2464 3234 3240 3246 3279 5921 6417
Size: 41 382 770 6 6 33 2642 496
Cuts at: 6417 2041
Size: 2342
Fragments arranged by size:
2642 2342 770 496 382 41 33 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3518 3773 1163
Size: 573 1782 255 4108
Fragments arranged by size:
4108 1782 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2027 2254 2424 4168 4929 5957
Size: 275 186 26 227 170 1744 761 1028
Cuts at: 5957 1540
Size: 2301
Fragments arranged by size:
2301 1744 1028 761 275 227 186 170
26
BsiHKAI G_wGCw'C
Cuts at: 3734 3768 3820 3960 4779 4864 5935 3734
Size: 34 52 140 819 85 1071 4517
Fragments arranged by size:
4517 1071 819 140 85 52 34
BsmI GAATG_Cn'
Cuts at: 2160 4229 4322 2160
Size: 2069 93 4556
Fragments arranged by size:
4556 2069 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2239 2271 2729 2766 2847 3566
Size: 399 299 150 32 458 37 81 719
Cuts at: 3566 3734 3768 3820 3960 3977 4037 4779 4864
Size: 168 34 52 140 17 60 742 85
Cuts at: 4864 5935 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 719 458 399 299 168
150 140 85 81 60 52 37 34
32 17
BspEI T'CCGG_A
Cuts at: 6381 6381
Size: 6718
BspHI T'CATG_A
Cuts at: 4607 6337 4607
Size: 1730 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3777 3861 3957 4073 844
Size: 2933 84 96 116 3489
Fragments arranged by size:
3489 2933 116 96 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3634 3718 3802 4605 1394
Size: 429 1811 84 84 803 3507
Fragments arranged by size:
3507 1811 803 429 84 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 5213 5387 65
Size: 1183 1362 2603 174 1396
Fragments arranged by size:
2603 1396 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 5362 5549 1700
Size: 110 315 3237 187 2869
Fragments arranged by size:
3237 2869 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6718
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6718
BssSI C'ACGA_G
Cuts at: 3559 4778 5790 3559
Size: 1219 1012 4487
Fragments arranged by size:
4487 1219 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3501 3853 794
Size: 2707 352 3659
Fragments arranged by size:
3659 2707 352
BstBI TT'CG_AA
Cuts at: 5522 5522
Size: 6718
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6718
BstXI CCAn_nnnn'nTGG
Cuts at: 3132 3307 3132
Size: 175 6543
BstYI r'GATC_y
Cuts at: 1972 2289 2824 3058 4048 4415 4800 4817 6269
Size: 317 535 234 990 367 385 17 1452
Cuts at: 6269 6355 6367 6713 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 990 535 385 367 346 317
234 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2424 3054 3131 3709 3986 513
Size: 552 1359 630 77 578 277 3245
Fragments arranged by size:
3245 1359 630 578 552 277 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3286 3450 487
Size: 683 56 2060 164 3755
Fragments arranged by size:
3755 2060 683 164 56
BtsI GCAGTG_nn'
Cuts at: 4234 5028 5048 4234
Size: 794 20 5904
Fragments arranged by size:
5904 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6683
DraI TTT'AAA
Cuts at: 4022 4022
Size: 6718
DraIII CAC_nnn'GTG
Cuts at: 2898 2898
Size: 6718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5725 809
Size: 507 4409 1802
Fragments arranged by size:
4409 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2145 2198
Size: 54 1425 50 275 18 194 121 53
Cuts at: 2198 2403 2421 3309 4165 5054 6683 8
Size: 205 18 888 856 889 1629 43
Fragments arranged by size:
1629 1425 889 888 856 275 205 194
121 54 53 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2024 2421 4165 1537
Size: 275 212 397 1744 4090
Fragments arranged by size:
4090 1744 397 275 212
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 3656 4648 1353
Size: 1446 857 992 3423
Fragments arranged by size:
3423 1446 992 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3535 3680 5305 5679 5825 387
Size: 1064 138 1946 145 1625 374 146 1280
Fragments arranged by size:
1946 1625 1280 1064 374 146 145 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2390 2528 2733 2942 4781 5378
Size: 393 466 292 138 205 209 1839 597
Cuts at: 5378 6165 6433 1239
Size: 787 268 1524
Fragments arranged by size:
1839 1524 787 597 466 393 292 268
209 205 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2684 2477
Size: 207 6511
EcoO109I rG'GnC_Cy
Cuts at: 2053 2469 2548 2581 2917 2977 3974 2053
Size: 416 79 33 336 60 997 4797
Fragments arranged by size:
4797 997 416 336 79 60 33
EcoRI G'AATT_C
Cuts at: 4027 4405 4027
Size: 378 6340
FspI TGC'GCA
Cuts at: 2531 2859 3593 5224 2531
Size: 328 734 1631 4025
Fragments arranged by size:
4025 1631 734 328
HaeII r_GCGC'y
Cuts at: 1678 2132 2797 3398 3543 3889 3984 5865 1678
Size: 454 665 601 145 346 95 1881 2531
Fragments arranged by size:
2531 1881 665 601 454 346 145 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 2969 3001 3083
Size: 32 135 32 1283 32 82 32 82
Cuts at: 3083 3115 5359 5391 5433 5465 1373
Size: 32 2244 32 42 32 2626
Fragments arranged by size:
2626 2244 1283 135 82 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2019 2608 4062 4308 669
Size: 988 281 81 589 1454 246 3079
Fragments arranged by size:
3079 1454 988 589 281 246 81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6718
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 6718
KasI G'GCGC_C
Cuts at: 3394 3539 3980 3394
Size: 145 441 6132
Fragments arranged by size:
6132 441 145
KpnI G_GTAC'C
Cuts at: 4043 4043
Size: 6718
MfeI C'AATT_G
Cuts at: 4317 4317
Size: 6718
MluI A'CGCG_T
Cuts at: 6594 6594
Size: 6718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5832 6016 1305
Size: 4527 184 2007
Fragments arranged by size:
4527 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4896
Fragments arranged by size:
4896 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3130 3305 4676 5035 5194 518
Size: 648 1449 515 175 1371 359 159 2042
Fragments arranged by size:
2042 1449 1371 648 515 359 175 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2070 2359 2914 3265 3498 3711 3784
Size: 435 29 289 555 351 233 213 73
Cuts at: 3784 3963 4134 4811 5959 6204 1606
Size: 179 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 435 351 289 245
233 213 179 171 73 29
NaeI GCC'GGC
Cuts at: 2127 2127
Size: 6718
NarI GG'CG_CC
Cuts at: 3395 3540 3981 3395
Size: 145 441 6132
Fragments arranged by size:
6132 441 145
NcoI C'CATG_G
Cuts at: 513 1065 3131 513
Size: 552 2066 4100
Fragments arranged by size:
4100 2066 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6718
NgoMIV G'CCGG_C
Cuts at: 2125 2125
Size: 6718
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6718
NotI GC'GGCC_GC
Cuts at: 4165 4165
Size: 6718
NspI r_CATG'y
Cuts at: 1132 1229 4076 5621 1132
Size: 97 2847 1545 2229
Fragments arranged by size:
2847 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2766 1348
Size: 1418 5300
PciI A'CATG_T
Cuts at: 5617 5617
Size: 6718
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 1108
Size: 948 5770
PfoI T'CCnGG_A
Cuts at: 2987 2987
Size: 6718
PmeI GTTT'AAAC
Cuts at: 4022 4022
Size: 6718
PmlI CAC'GTG
Cuts at: 3897 3897
Size: 6718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 6300 6332 4787
Size: 32 1481 32 5173
Fragments arranged by size:
5173 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2469 2548 2977 2469
Size: 79 429 6210
Fragments arranged by size:
6210 429 79
PshAI GACnn'nnGTC
Cuts at: 1613 2603 1613
Size: 990 5728
PsiI TTA'TAA
Cuts at: 2673 4288 2673
Size: 1615 5103
PspOMI G'GGCC_C
Cuts at: 2085 3973 2085
Size: 1888 4830
PstI C_TGCA'G
Cuts at: 830 2481 2499 3270 3774 4070 830
Size: 1651 18 771 504 296 3478
Fragments arranged by size:
3478 1651 771 504 296 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6718
PvuII CAG'CTG
Cuts at: 2041 2359 3265 3498 2041
Size: 318 906 233 5261
Fragments arranged by size:
5261 906 318 233
SacII CC_GC'GG
Cuts at: 3712 3712
Size: 6718
SalI G'TCGA_C
Cuts at: 1936 2606 4060 1936
Size: 670 1454 4594
Fragments arranged by size:
4594 1454 670
SanDI GG'GwC_CC
Cuts at: 2469 2469
Size: 6718
SapI GCTCTTCn'nnn_
Cuts at: 3656 3656
Size: 6718
SbfI CC_TGCA'GG
Cuts at: 4070 4070
Size: 6718
ScaI AGT'ACT
Cuts at: 4966 6678 4966
Size: 1712 5006
SfcI C'TryA_G
Cuts at: 826 1045 2477 2495 3266 3751 3770 4066 5201
Size: 219 1432 18 771 485 19 296 1135
Cuts at: 5201 5882 6073 826
Size: 681 191 1471
Fragments arranged by size:
1471 1432 1135 771 681 485 296 219
191 19 18
SfoI GGC'GCC
Cuts at: 3396 3541 3982 3396
Size: 145 441 6132
Fragments arranged by size:
6132 441 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6718
SmaI CCC'GGG
Cuts at: 1872 2565 3321 3373 3972 4045 1872
Size: 693 756 52 599 73 4545
Fragments arranged by size:
4545 756 693 599 73 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2172 3857 3938 4822 5723 5985
Size: 197 926 229 1685 81 884 901 262
Cuts at: 5985 820
Size: 1553
Fragments arranged by size:
1685 1553 926 901 884 262 229 197
81
SnaBI TAC'GTA
Cuts at: 493 4509 493
Size: 4016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6718
SphI G_CATG'C
Cuts at: 1132 4076 1132
Size: 2944 3774
SrfI GCCC'GGGC
Cuts at: 3972 3972
Size: 6718
SspI AAT'ATT
Cuts at: 5 52 4642 5
Size: 47 4590 2081
Fragments arranged by size:
4590 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6718
StyI C'CwwG_G
Cuts at: 513 1065 2586 3131 3627 4157 513
Size: 552 1521 545 496 530 3074
Fragments arranged by size:
3074 1521 552 545 530 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4745 4762 4915 5100 5545 325
Size: 1255 3165 17 153 185 445 1498
Fragments arranged by size:
3165 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 4964 6676
Size: 274 80 33 51 1443 642 2345 1712
Cuts at: 6676 96
Size: 138
Fragments arranged by size:
2345 1712 1443 642 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2229 2261 2438 2470 2882 2914 2996 3028 3978
Size: 32 177 32 412 32 82 32 950
Cuts at: 3978 4010 6678 6710 2229
Size: 32 2668 32 2237
Fragments arranged by size:
2668 2237 950 412 177 82 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6718
XbaI T'CTAG_A
Cuts at: 4054 4054
Size: 6718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 6718
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6718
XmaI C'CCGG_G
Cuts at: 1870 2563 3319 3371 3970 4043 1870
Size: 693 756 52 599 73 4545
Fragments arranged by size:
4545 756 693 599 73 52
XmnI GAAnn'nnTTC
Cuts at: 3665 4847 3665
Size: 1182 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4526 276
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SexAI SfiI
SwaI