(Circular) (Six-base) MAPSORT of: pFN21AB6010.seq Check: 1412 from: 1 to: 8398 pFN21AB6010 8398 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6208 278 Size: 53 83 186 936 4672 2468 Fragments arranged by size: 4672 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4448 5741 1937 Size: 2511 1293 4594 Fragments arranged by size: 4594 2511 1293 Acc65I G'GTAC_C Cuts at: 5719 5719 Size: 8398 AclI AA'CG_TT Cuts at: 1563 6525 6898 1563 Size: 4962 373 3063 Fragments arranged by size: 4962 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 2810 3298 4381 5304 5654 6461 7845 Size: 840 338 488 1083 923 350 807 1384 Cuts at: 7845 8113 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1083 923 840 807 488 350 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8201 AflIII A'CryG_T Cuts at: 1203 4428 7297 8274 1203 Size: 3225 2869 977 1327 Fragments arranged by size: 3225 2869 1327 977 AgeI A'CCGG_T Cuts at: 7229 7229 Size: 8398 AhdI GACnn_n'nnGTC Cuts at: 5173 7127 5173 Size: 1954 6444 AleI CACnn'nnGTG Cuts at: 1166 4207 1166 Size: 3041 5357 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3576 3849 1184 Size: 2392 273 5733 Fragments arranged by size: 5733 2392 273 AlwNI CAG_nnn'CTG Cuts at: 6141 7713 6141 Size: 1572 6826 ApaI G_GGCC'C Cuts at: 2153 2153 Size: 8398 ApaLI G'TGCA_C Cuts at: 6455 7611 6455 Size: 1156 7242 ApoI r'AATT_y Cuts at: 1493 2456 2534 2913 3293 3995 4591 4740 4940 Size: 963 78 379 380 702 596 149 200 Cuts at: 4940 4990 5018 5140 5707 5933 6085 8194 1493 Size: 50 28 122 567 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 702 596 567 380 379 226 200 152 149 122 78 50 28 AseI AT'TA_AT Cuts at: 160 1032 2721 6952 160 Size: 872 1689 4231 1606 Fragments arranged by size: 4231 1689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8398 AvaI C'yCGr_G Cuts at: 1870 1943 5723 1870 Size: 73 3780 4545 Fragments arranged by size: 4545 3780 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3870 3903 3870 Size: 33 8365 BamHI G'GATC_C Cuts at: 5728 6095 5728 Size: 367 8031 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5719 7174 618 Size: 325 217 627 3932 1455 1842 Fragments arranged by size: 3932 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2153 5391 5717 1391 Size: 762 3238 326 4072 Fragments arranged by size: 4072 3238 762 326 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3367 3671 4495 928 Size: 971 1468 304 824 4831 Fragments arranged by size: 4831 1468 971 824 304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2907 2941 6589 6623 1903 Size: 34 970 34 3648 34 3678 Fragments arranged by size: 3678 3648 970 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2590 6290 7500 2590 Size: 3700 1210 3488 Fragments arranged by size: 3700 3488 1210 BclI T'GATC_A Cuts at: 1548 4888 1548 Size: 3340 5058 BfrBI ATG'CAT Cuts at: 3013 4055 3013 Size: 1042 7356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7009 136 Size: 107 122 71 631 605 5337 1525 Fragments arranged by size: 5337 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8393 8393 Size: 8398 BlpI GC'TnA_GC Cuts at: 4481 5815 4481 Size: 1334 7064 Bme1580I G_kGCm'C Cuts at: 1790 2153 6459 7615 1790 Size: 363 4306 1156 2573 Fragments arranged by size: 4306 2573 1156 363 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3496 6129 7087 Size: 416 53 83 399 24 2079 2633 958 Cuts at: 7087 442 Size: 1753 Fragments arranged by size: 2633 2079 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5153 1056 Size: 4097 4301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3314 Size: 8398 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2001 3309 7058 1239 Size: 762 1308 3749 2579 Fragments arranged by size: 3749 2579 1308 762 Bpu10I CC'TnA_GC Cuts at: 4182 4182 Size: 8398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6523 7388 7686 6523 Size: 865 298 7235 Fragments arranged by size: 7235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7061 882 Size: 617 5562 2219 Fragments arranged by size: 5562 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6189 493 Size: 711 4985 2702 Fragments arranged by size: 4985 2702 711 BsaBI GATnn'nnATC Cuts at: 2037 4056 5469 8392 2037 Size: 2019 1413 2923 2043 Fragments arranged by size: 2923 2043 2019 1413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6205 6587 7222 275 Size: 53 83 186 936 4672 382 635 1451 Fragments arranged by size: 4672 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3852 6831 7229 7503 7650 8061 3852 Size: 2979 398 274 147 411 4189 Fragments arranged by size: 4189 2979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2868 2898 1178 Size: 30 165 30 1465 30 6678 Fragments arranged by size: 6678 1465 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2196 3347 1186 Size: 1010 1151 6237 Fragments arranged by size: 6237 1151 1010 BseYI C'CCAG_C Cuts at: 2152 7601 8097 2152 Size: 5449 496 2453 Fragments arranged by size: 5449 2453 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4554 4562 5318 1163 Size: 573 2818 8 756 4243 Fragments arranged by size: 4243 2818 756 573 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5848 6609 7637 1540 Size: 275 186 3847 761 1028 2301 Fragments arranged by size: 3847 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3565 6459 6544 7615 3565 Size: 2894 85 1071 4348 Fragments arranged by size: 4348 2894 1071 85 BsmI GAATG_Cn' Cuts at: 2866 5909 6002 2866 Size: 3043 93 5262 Fragments arranged by size: 5262 3043 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 3565 5391 5717 6459 6544 7615 Size: 399 363 1412 1826 326 742 85 1071 Cuts at: 7615 1391 Size: 2174 Fragments arranged by size: 2174 1826 1412 1071 742 399 363 326 85 BspEI T'CCGG_A Cuts at: 3852 8061 3852 Size: 4209 4189 BspHI T'CATG_A Cuts at: 5079 5418 6287 8017 5079 Size: 339 869 1730 5460 Fragments arranged by size: 5460 1730 869 339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5753 844 Size: 4909 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6285 1394 Size: 429 4462 3507 Fragments arranged by size: 4462 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2564 4463 4911 6893 7067 65 Size: 1183 1316 1899 448 1982 174 1396 Fragments arranged by size: 1982 1899 1396 1316 1183 448 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3267 4002 7042 7229 1700 Size: 110 1457 735 3040 187 2869 Fragments arranged by size: 3040 2869 1457 735 187 110 BsrGI T'GTAC_A Cuts at: 96 2884 96 Size: 2788 5610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8398 BssSI C'ACGA_G Cuts at: 6458 7470 6458 Size: 1012 7386 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8398 BstBI TT'CG_AA Cuts at: 2440 7202 2440 Size: 4762 3636 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8398 BstXI CCAn_nnnn'nTGG Cuts at: 2234 2234 Size: 8398 BstYI r'GATC_y Cuts at: 1972 3325 3849 5554 5728 6095 6480 6497 7949 Size: 1353 524 1705 174 367 385 17 1452 Cuts at: 7949 8035 8047 8393 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1705 1452 1353 524 385 367 346 174 86 17 12 BstZ17I GTA'TAC Cuts at: 4449 4449 Size: 8398 Bsu36I CC'TnA_GG Cuts at: 5406 5406 Size: 8398 BtgI C'CryG_G Cuts at: 513 1065 2490 3618 3708 5583 513 Size: 552 1425 1128 90 1875 3328 Fragments arranged by size: 3328 1875 1425 1128 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7659 Fragments arranged by size: 7659 683 56 BtsI GCAGTG_nn' Cuts at: 3219 4086 4202 4214 5330 5914 6708 6728 3219 Size: 867 116 12 1116 584 794 20 4889 Fragments arranged by size: 4889 1116 867 794 584 116 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8363 DraI TTT'AAA Cuts at: 2872 2991 4287 4951 4989 5702 2872 Size: 119 1296 664 38 713 5568 Fragments arranged by size: 5568 1296 713 664 119 38 DraIII CAC_nnn'GTG Cuts at: 3527 3527 Size: 8398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2636 3042 7405 809 Size: 507 1320 406 4363 1802 Fragments arranged by size: 4363 1802 1320 507 406 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3269 5845 6734 Size: 54 1425 50 275 18 1439 2576 889 Cuts at: 6734 8363 8 Size: 1629 43 Fragments arranged by size: 2576 1629 1439 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5845 1537 Size: 275 4033 4090 Fragments arranged by size: 4090 4033 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 3317 3349 3510 3619 3924 4073 5098 Size: 1575 389 32 161 109 305 149 1025 Cuts at: 5098 5499 6328 1353 Size: 401 829 3423 Fragments arranged by size: 3423 1575 1025 829 401 389 305 161 149 109 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6985 7359 7505 387 Size: 1064 138 5396 374 146 1280 Fragments arranged by size: 5396 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2472 2810 3298 3309 4381 5304 Size: 393 369 471 338 488 11 1072 923 Cuts at: 5304 5654 6461 7058 7845 8113 1239 Size: 350 807 597 787 268 1524 Fragments arranged by size: 1524 1072 923 807 787 597 488 471 393 369 350 338 268 11 EcoO109I rG'GnC_Cy Cuts at: 2149 2149 Size: 8398 EcoRI G'AATT_C Cuts at: 2456 2534 5707 6085 2456 Size: 78 3173 378 4769 Fragments arranged by size: 4769 3173 378 78 EcoRV GAT'ATC Cuts at: 2092 4657 4699 2092 Size: 2565 42 5791 Fragments arranged by size: 5791 2565 42 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3539 4291 3539 Size: 752 7646 FspI TGC'GCA Cuts at: 6904 6904 Size: 8398 HaeII r_GCGC'y Cuts at: 1678 7545 1678 Size: 5867 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 4686 4718 4811 Size: 32 135 32 427 32 2655 32 93 Cuts at: 4811 4843 5159 5191 5413 5431 5445 5463 5539 Size: 32 316 32 222 18 14 18 76 Cuts at: 5539 5571 7039 7071 7113 7145 1373 Size: 32 1468 32 42 32 2626 Fragments arranged by size: 2655 2626 1468 427 316 222 135 93 76 42 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3971 4071 4322 5208 5742 5988 Size: 988 281 2033 100 251 886 534 246 Cuts at: 5988 669 Size: 3079 Fragments arranged by size: 3079 2033 988 886 534 281 251 246 100 HindIII A'AGCT_T Cuts at: 748 748 Size: 8398 HpaI GTT'AAC Cuts at: 5988 5988 Size: 8398 KpnI G_GTAC'C Cuts at: 5723 5723 Size: 8398 MfeI C'AATT_G Cuts at: 5511 5997 5511 Size: 486 7912 MluI A'CGCG_T Cuts at: 8274 8274 Size: 8398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2984 3654 7512 7696 1305 Size: 1679 670 3858 184 2007 Fragments arranged by size: 3858 2007 1679 670 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6576 Fragments arranged by size: 6576 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2723 2948 3429 3831 4207 4916 Size: 648 1377 180 225 481 402 376 709 Cuts at: 4916 4929 5378 6356 6715 6874 518 Size: 13 449 978 359 159 2042 Fragments arranged by size: 2042 1377 978 709 648 481 449 402 376 359 225 180 159 13 MspA1I CmG'CkG Cuts at: 1606 3287 4649 5814 6491 7639 7884 1606 Size: 1681 1362 1165 677 1148 245 2120 Fragments arranged by size: 2120 1681 1362 1165 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2490 5583 513 Size: 552 1425 3093 3328 Fragments arranged by size: 3328 3093 1425 552 NdeI CA'TA_TG Cuts at: 387 2418 387 Size: 2031 6367 NheI G'CTAG_C Cuts at: 1052 5149 1052 Size: 4097 4301 NotI GC'GGCC_GC Cuts at: 5845 5845 Size: 8398 NsiI A_TGCA'T Cuts at: 3015 4057 3015 Size: 1042 7356 NspI r_CATG'y Cuts at: 1132 1229 3434 3457 3900 4180 4432 5269 5756 Size: 97 2205 23 443 280 252 837 487 Cuts at: 5756 7301 1132 Size: 1545 2229 Fragments arranged by size: 2229 2205 1545 837 487 443 280 252 97 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8398 PciI A'CATG_T Cuts at: 4428 7297 4428 Size: 2869 5529 PflMI CCAn_nnn'nTGG Cuts at: 1108 3527 1108 Size: 2419 5979 PmeI GTTT'AAAC Cuts at: 5702 5702 Size: 8398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6467 6499 7980 8012 6467 Size: 32 1481 32 6853 Fragments arranged by size: 6853 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8398 PsiI TTA'TAA Cuts at: 3072 4683 5469 5968 3072 Size: 1611 786 499 5502 Fragments arranged by size: 5502 1611 786 499 PspOMI G'GGCC_C Cuts at: 2149 2149 Size: 8398 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2790 2822 2790 Size: 32 8366 PstI C_TGCA'G Cuts at: 830 3226 4209 4654 5750 830 Size: 2396 983 445 1096 3478 Fragments arranged by size: 3478 2396 1096 983 445 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8398 PvuII CAG'CTG Cuts at: 3287 4649 3287 Size: 1362 7036 SalI G'TCGA_C Cuts at: 1936 5740 1936 Size: 3804 4594 SapI GCTCTTCn'nnn_ Cuts at: 3317 3510 5499 3317 Size: 193 1989 6216 Fragments arranged by size: 6216 1989 193 SbfI CC_TGCA'GG Cuts at: 5750 5750 Size: 8398 ScaI AGT'ACT Cuts at: 3785 6646 8358 3785 Size: 2861 1712 3825 Fragments arranged by size: 3825 2861 1712 SfcI C'TryA_G Cuts at: 826 1045 2045 3222 3741 4205 4650 5238 5746 Size: 219 1000 1177 519 464 445 588 508 Cuts at: 5746 6881 7562 7753 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 1000 681 588 519 508 464 445 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8398 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8398 SmaI CCC'GGG Cuts at: 1872 5725 1872 Size: 3853 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6502 7403 7665 820 Size: 197 926 4559 901 262 1553 Fragments arranged by size: 4559 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6189 493 Size: 5696 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8398 SphI G_CATG'C Cuts at: 1132 3434 5269 5756 1132 Size: 2302 1835 487 3774 Fragments arranged by size: 3774 2302 1835 487 SspI AAT'ATT Cuts at: 5 52 2868 3859 6322 5 Size: 47 2816 991 2463 2081 Fragments arranged by size: 2816 2463 2081 991 47 StuI AGG'CCT Cuts at: 1517 4364 1517 Size: 2847 5551 StyI C'CwwG_G Cuts at: 513 1065 2490 5583 5837 513 Size: 552 1425 3093 254 3074 Fragments arranged by size: 3093 3074 1425 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2346 6425 6442 6595 6780 7225 325 Size: 1255 766 4079 17 153 185 445 1498 Fragments arranged by size: 4079 1498 1255 766 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2884 3166 3783 Size: 274 80 33 51 1443 907 282 617 Cuts at: 3783 4211 6644 8356 96 Size: 428 2433 1712 138 Fragments arranged by size: 2433 1712 1443 907 617 428 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3440 3472 3627 3659 8358 8390 3440 Size: 32 155 32 4699 32 3448 Fragments arranged by size: 4699 3448 155 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3366 1316 Size: 2050 6348 XbaI T'CTAG_A Cuts at: 3409 5734 3409 Size: 2325 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8398 XmaI C'CCGG_G Cuts at: 1870 5723 1870 Size: 3853 4545 XmnI GAAnn'nnTTC Cuts at: 2513 3213 4017 5676 6527 2513 Size: 700 804 1659 851 4384 Fragments arranged by size: 4384 1659 851 804 700 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6206 276 Size: 53 83 186 936 4672 2468 Fragments arranged by size: 4672 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BsiWI BsmBI EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI