##################################
# NO CUT ENZYMES (  230 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AcsI           20  AAATTC               [GA]^AATT[CT]
ApoI           20  AAATTC               [GA]^AATT[CT]
BpmI          203  CTCCAG               CTGGAG(16/14)
BsgI          120  CTGCAC               GTGCAG(16/14)
BsmAI          27  GTCTC                GTCTC(1/5)
BspMI         118  ACCTGC               ACCTGC(4/8)
BssSI           5  CTCGTG               CACGAG(-5/-1)
BstF5I        184  CATCC                GGATG(2/0)
FokI          184  CATCC                GGATG(9/13)
HinfI         192  GACTC                G^A[ACGT]TC
MboII          66  GAAGA                GAAGA(8/7)
MnlI          202  CCTC                 CCTC(7/6)
MseI          109  TTAA                 T^TAA
NlaIII         79  CATG                 CATG^
PleI          192  GACTC                GAGTC(4/5)
SfaNI         183  GCATC                GCATC(5/9)
SfcI          129  CTGTAG               C^T[GA][CT]AG
Tru9I         109  TTAA                 T^TAA
TspRI         123  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

Cac8I          51  GCAAGC               GC[ACGT]^[ACGT]GC
              179  GCAAGC              
MwoI           94  GCTGCCAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               97  GCCAAAGCTGC         
Tsp509I        21  AATT                 ^AATT
              209  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AluI        BbvI        DdeI        Fnu4HI      ItaI        
TseI