################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BciVI BfrI BlnI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 2029 GACGTC GACGT^C AgeI 928 ACCGGT A^CCGGT AocI 223 CCTGAGG CC^T[ACGT]AGG Aor51HI 1496 AGCGCT AGC^GCT AvaI 137 CTCGAG C^[CT]CG[GA]G BanI 964 GGTGCC G^G[CT][GA]CC BbsI 1816 GAAGAC GAAGAC(2/6) BbvCI 2962 GCTGAGG CCTCAGC(-5/-2) BcgI 1712 GCAACTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1482 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1482 GCTGAGC GC^T[ACGT]AGC BpuAI 1816 GAAGAC GAAGAC(2/6) BsaWI 928 ACCGGT [AT]^CCGG[AT] BsiEI 1435 CGGCCG CG[GA][CT]^CG BsmI 2233 GAATGC GAATGC(1/-1) BsoBI 137 CTCGAG C^[CT]CG[GA]G Bsp1407I 292 TGTACA T^GTACA BsrGI 292 TGTACA T^GTACA BstEII 155 GGTCACC G^GT[ACGT]ACC BstPI 155 GGTCACC G^GT[ACGT]ACC Bsu36I 223 CCTGAGG CC^T[ACGT]AGG CelII 1482 GCTGAGC GC^T[ACGT]AGC DraIII 1286 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1435 CGGCCG C^GGCCG EclXI 1435 CGGCCG C^GGCCG Eco47III 1496 AGCGCT AGC^GCT Eco52I 1435 CGGCCG C^GGCCG Eco81I 223 CCTGAGG CC^T[ACGT]AGG EcoNI 1719 CCTCGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 155 GGTCACC G^GT[ACGT]ACC EcoRI 582 GAATTC G^AATTC EcoRV 1700 GATATC GAT^ATC EcoT22I 796 ATGCAT ATGCA^T FseI 1436 GGCCGGCC GGCCGG^CC NaeI 1437 GCCGGC GCC^GGC NgoMIV 1437 GCCGGC G^CCGGC NheI 2214 GCTAGC G^CTAGC NsiI 796 ATGCAT ATGCA^T PaeR7I 137 CTCGAG C^TCGAG PinAI 928 ACCGGT A^CCGGT ScaI 2804 AGTACT AGT^ACT SmlI 137 CTCGAG C^T[CT][GA]AG SspBI 292 TGTACA T^GTACA XbaI 2613 TCTAGA T^CTAGA XhoI 137 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 721 CGCG CG^CG 2551 CGCG AlwI 2304 GATCC GGATC(4/5) 843 GGATC ApaI 508 GGGCCC GGGCC^C 2967 GGGCCC Asp700I 1407 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1851 GAAACAGTTC BbrPI 2308 CACGTG CAC^GTG 2433 CACGTG BclI 500 TGATCA T^GATCA 2225 TGATCA BsaAI 2308 CACGTG [CT]AC^GT[GA] 2433 CACGTG BsaI 1903 GAGACC GGTCTC(1/5) 104 GGTCTC BseRI 140 GAGGAG GAGGAG(10/8) 2344 GAGGAG BspHI 1185 TCATGA T^CATGA 1583 TCATGA BsrDI 2958 CATTGC GCAATG(2/0) 908 GCAATG BsrFI 928 ACCGGT [GA]^CCGG[CT] 1437 GCCGGC BstUI 721 CGCG CG^CG 2551 CGCG BstXI 1289 CCAGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2066 CCATGAACCTGG Cfr10I 928 ACCGGT [GA]^CCGG[CT] 1437 GCCGGC DrdI 298 GACAACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1615 GACAAGGCTGTC DsaI 459 CCACGG C^C[GA][CT]GG 2744 CCGTGG EarI 1587 GAAGAG CTCTTC(1/4) 2047 GAAGAG FbaI 500 TGATCA T^GATCA 2225 TGATCA HaeII 1496 AGCGCT [GA]GCGC^[CT] 2564 AGCGCC HincII 1807 GTCAAC GT[CT]^[GA]AC 2035 GTCAAC HindII 1807 GTCAAC GT[CT]^[GA]AC 2035 GTCAAC Ksp632I 1587 GAAGAG CTCTTC(1/4) 2047 GAAGAG MvnI 721 CGCG CG^CG 2551 CGCG NspI 728 GCATGT [GA]CATG^[CT] 1119 GCATGT PflMI 616 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2763 CCAGCTCCTGG PmaCI 2308 CACGTG CAC^GTG 2433 CACGTG PmlI 2308 CACGTG CAC^GTG 2433 CACGTG RcaI 1185 TCATGA T^CATGA 1583 TCATGA Van91I 616 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2763 CCAGCTCCTGG XmnI 1407 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1851 GAAACAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwNI ApoI AspHI AspI AvaII BanII BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII