Restriction map for
pFN21AE2563
(Circular) (Six-base) MAPSORT of: pFN21AE2563.seq Check: 6142 from: 1 to: 7690
pFN21AE2563 7690 bp
With 182 enzymes: SgfI *
March 4, 2015 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2094 2608 2094
Size: 514 7176
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4038 5500 278
Size: 53 83 186 936 2502 1462 2468
Fragments arranged by size:
2502 2468 1462 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5033 1937
Size: 3096 4594
Acc65I G'GTAC_C
Cuts at: 5011 5011
Size: 7690
AclI AA'CG_TT
Cuts at: 1563 5817 6190 1563
Size: 4254 373 3063
Fragments arranged by size:
4254 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2714 4520 5753 7137 7405 1632
Size: 1082 1806 1233 1384 268 1917
Fragments arranged by size:
1917 1806 1384 1233 1082 268
AfeI AGC'GCT
Cuts at: 3503 3503
Size: 7690
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7493
AflIII A'CryG_T
Cuts at: 1203 6589 7566 1203
Size: 5386 977 1327
Fragments arranged by size:
5386 1327 977
AgeI A'CCGG_T
Cuts at: 2933 6521 2933
Size: 3588 4102
AhdI GACnn_n'nnGTC
Cuts at: 6419 6419
Size: 7690
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7690
AlwNI CAG_nnn'CTG
Cuts at: 3676 3720 3952 4701 4774 5433 7005 3676
Size: 44 232 749 73 659 1572 4361
Fragments arranged by size:
4361 1572 749 659 232 73 44
ApaI G_GGCC'C
Cuts at: 2517 4976 2517
Size: 2459 5231
ApaLI G'TGCA_C
Cuts at: 5747 6903 5747
Size: 1156 6534
ApoI r'AATT_y
Cuts at: 1493 2587 2807 2824 4999 5225 5377 7486 1493
Size: 1094 220 17 2175 226 152 2109 1697
Fragments arranged by size:
2175 2109 1697 1094 226 220 152 17
AseI AT'TA_AT
Cuts at: 160 1032 6244 160
Size: 872 5212 1606
Fragments arranged by size:
5212 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7690
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 5015 1870
Size: 73 199 2873 4545
Fragments arranged by size:
4545 2873 199 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3186 3219 3186
Size: 33 7657
BamHI G'GATC_C
Cuts at: 5020 5387 5020
Size: 367 7323
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2969 5011 6466 618
Size: 325 217 627 1182 2042 1455 1842
Fragments arranged by size:
2042 1842 1455 1182 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2464 2517 2619 2982 3138 4976 5009 1391
Size: 1073 53 102 363 156 1838 33 4072
Fragments arranged by size:
4072 1838 1073 363 156 102 53 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3828 928
Size: 971 1929 4790
Fragments arranged by size:
4790 1929 971
BbvCI CC'TCA_GC
Cuts at: 4968 4968
Size: 7690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 5881 5915 1903
Size: 34 1769 34 2141 34 3678
Fragments arranged by size:
3678 2141 1769 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5582 6792 5582
Size: 1210 6480
BclI T'GATC_A
Cuts at: 1548 2505 4230 1548
Size: 957 1725 5008
Fragments arranged by size:
5008 1725 957
BfrBI ATG'CAT
Cuts at: 2803 2803
Size: 7690
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 3397 3965
Size: 107 122 71 631 605 614 1111 568
Cuts at: 3965 6301 136
Size: 2336 1525
Fragments arranged by size:
2336 1525 1111 631 614 605 568 122
107 71
BglII A'GATC_T
Cuts at: 2059 2578 3067 4462 4635 7685 2059
Size: 519 489 1395 173 3050 2064
Fragments arranged by size:
3050 2064 1395 519 489 173
BlpI GC'TnA_GC
Cuts at: 3488 5107 3488
Size: 1619 6071
Bme1580I G_kGCm'C
Cuts at: 1790 2517 4976 5751 6907 1790
Size: 727 2459 775 1156 2573
Fragments arranged by size:
2573 2459 1156 775 727
BmgBI CAC'GTC
Cuts at: 1318 3655 1318
Size: 2337 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4176 4273
Size: 416 53 83 399 24 1871 888 97
Cuts at: 4273 5421 6379 442
Size: 1148 958 1753
Fragments arranged by size:
1871 1753 1148 958 888 416 399 97
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4223 1056
Size: 3167 4523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2384 3559 2254
Size: 130 1175 6385
Fragments arranged by size:
6385 1175 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 3482 4367 4385 4429 6350 1239
Size: 1328 915 885 18 44 1921 2579
Fragments arranged by size:
2579 1921 1328 915 885 44 18
Bpu10I CC'TnA_GC
Cuts at: 2219 3879 4212 4968 2219
Size: 1660 333 756 4941
Fragments arranged by size:
4941 1660 756 333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5815 6680 6978 5815
Size: 865 298 6527
Fragments arranged by size:
6527 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2115 3902 6353 882
Size: 617 616 1787 2451 2219
Fragments arranged by size:
2451 2219 1787 617 616
BsaAI yAC'GTr
Cuts at: 493 1204 4315 4440 5481 493
Size: 711 3111 125 1041 2702
Fragments arranged by size:
3111 2702 1041 711 125
BsaBI GATnn'nnATC
Cuts at: 7684 7684
Size: 7690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2993 4035 4067 5497
Size: 53 83 186 936 1460 1042 32 1430
Cuts at: 5497 5879 6514 275
Size: 382 635 1451
Fragments arranged by size:
1460 1451 1430 1042 936 635 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 2933 6123 6521 6795 6942 7353 2933
Size: 3190 398 274 147 411 3270
Fragments arranged by size:
3270 3190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2522 2552 2920 2950 1178
Size: 30 165 30 1119 30 368 30 5918
Fragments arranged by size:
5918 1119 368 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2160 4364 1186
Size: 974 2204 4512
Fragments arranged by size:
4512 2204 974
BseYI C'CCAG_C
Cuts at: 3448 4767 6893 7389 3448
Size: 1319 2126 496 3749
Fragments arranged by size:
3749 2126 1319 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3983 4616 4941 1163
Size: 573 2247 633 325 3912
Fragments arranged by size:
3912 2247 633 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3443 5140 5901 6929 1540
Size: 275 186 1442 1697 761 1028 2301
Fragments arranged by size:
2301 1697 1442 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 2684 4445 5751 5836 6907 2664
Size: 20 1761 1306 85 1071 3447
Fragments arranged by size:
3447 1761 1306 1071 85 20
BsmI GAATG_Cn'
Cuts at: 4244 5201 5294 4244
Size: 957 93 6640
Fragments arranged by size:
6640 957 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 2517 2619 2664 2684 2982 3138
Size: 399 674 53 102 45 20 298 156
Cuts at: 3138 4445 4976 5009 5751 5836 6907 1391
Size: 1307 531 33 742 85 1071 2174
Fragments arranged by size:
2174 1307 1071 742 674 531 399 298
156 102 85 53 45 33 20
BspEI T'CCGG_A
Cuts at: 7353 7353
Size: 7690
BspHI T'CATG_A
Cuts at: 3190 3588 5579 7309 3190
Size: 398 1991 1730 3571
Fragments arranged by size:
3571 1991 1730 398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2608 2950 5045 844
Size: 1250 514 342 2095 3489
Fragments arranged by size:
3489 2095 1250 514 342
BsrBI CCG'CTC
Cuts at: 1394 1823 5577 1394
Size: 429 3754 3507
Fragments arranged by size:
3754 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2920 4962 6185 6359 65
Size: 1183 1672 2042 1223 174 1396
Fragments arranged by size:
2042 1672 1396 1223 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2933 3442 6334 6521 1700
Size: 110 1123 509 2892 187 2869
Fragments arranged by size:
2892 2869 1123 509 187 110
BsrGI T'GTAC_A
Cuts at: 96 2297 96
Size: 2201 5489
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7690
BssSI C'ACGA_G
Cuts at: 2112 2404 2711 4140 4265 5750 6762 2112
Size: 292 307 1429 125 1485 1012 3040
Fragments arranged by size:
3040 1485 1429 1012 307 292 125
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2688 3720 3952 3961 4931 794
Size: 1894 1032 232 9 970 3553
Fragments arranged by size:
3553 1894 1032 970 232 9
BstBI TT'CG_AA
Cuts at: 6494 6494
Size: 7690
BstEII G'GTnAC_C
Cuts at: 1184 2160 1184
Size: 976 6714
BstXI CCAn_nnnn'nTGG
Cuts at: 3301 4078 3301
Size: 777 6913
BstYI r'GATC_y
Cuts at: 1972 2059 2578 3067 4308 4462 4635 5020 5387
Size: 87 519 489 1241 154 173 385 367
Cuts at: 5387 5772 5789 7241 7327 7339 7685 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1241 519 489 385 385 367
346 173 154 87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2229
Size: 7690
BtgI C'CryG_G
Cuts at: 513 1065 2464 4749 513
Size: 552 1399 2285 3454
Fragments arranged by size:
3454 2285 1399 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3438 487
Size: 683 56 2212 4739
Fragments arranged by size:
4739 2212 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2263 2561 2771 3509 4089 4604 4797 5206
Size: 60 298 210 738 580 515 193 409
Cuts at: 5206 6000 6020 2203
Size: 794 20 3873
Fragments arranged by size:
3873 794 738 580 515 409 298 210
193 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7690
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2620 2655 4251 4286 529
Size: 35 2056 35 1596 35 3933
Fragments arranged by size:
3933 2056 1596 35 35 35
DraI TTT'AAA
Cuts at: 4994 4994
Size: 7690
DraIII CAC_nnn'GTG
Cuts at: 3296 3296
Size: 7690
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2309 3626 6697 809
Size: 507 993 1317 3071 1802
Fragments arranged by size:
3071 1802 1317 993 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3440 3477 3525
Size: 54 1425 50 275 18 1610 37 48
Cuts at: 3525 3984 5137 6026 7655 8
Size: 459 1153 889 1629 43
Fragments arranged by size:
1629 1610 1425 1153 889 459 275 54
50 48 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 3440 5137 1537
Size: 275 1628 1697 4090
Fragments arranged by size:
4090 1697 1628 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3587 4047 5620 1353
Size: 2234 460 1573 3423
Fragments arranged by size:
3423 2234 1573 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2503 3506 3860 6277 6651 6797
Size: 1064 138 914 1003 354 2417 374 146
Cuts at: 6797 387
Size: 1280
Fragments arranged by size:
2417 1280 1064 1003 914 374 354 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2714 3482 4367 4385 4429 4520
Size: 393 935 147 768 885 18 44 91
Cuts at: 4520 5753 6350 7137 7405 1239
Size: 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 935 885 787 768 597 393
268 147 91 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3728 3728
Size: 7690
EcoO109I rG'GnC_Cy
Cuts at: 2746 2962 3375 3871 4003 4186 4396 4698 4972
Size: 216 413 496 132 183 210 302 274
Cuts at: 4972 2746
Size: 5464
Fragments arranged by size:
5464 496 413 302 274 216 210 183
132
EcoRI G'AATT_C
Cuts at: 2587 4999 5377 2587
Size: 2412 378 4900
Fragments arranged by size:
4900 2412 378
EcoRV GAT'ATC
Cuts at: 3707 3707
Size: 7690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3110 3646 3110
Size: 536 7154
FseI GG_CCGG'CC
Cuts at: 3446 3446
Size: 7690
FspI TGC'GCA
Cuts at: 6196 6196
Size: 7690
HaeII r_GCGC'y
Cuts at: 1678 3505 4573 6837 1678
Size: 1827 1068 2264 2531
Fragments arranged by size:
2531 2264 1827 1068
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 2946 2978 3006
Size: 32 135 32 950 32 392 32 28
Cuts at: 3006 3038 3143 3175 3302 3334 6331 6363 6405
Size: 32 105 32 127 32 2997 32 42
Cuts at: 6405 6437 1373
Size: 32 2626
Fragments arranged by size:
2997 2626 950 392 135 127 105 42
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3814 4042 5034 5280 669
Size: 988 281 1876 228 992 246 3079
Fragments arranged by size:
3079 1876 992 988 281 246 228
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7690
HpaI GTT'AAC
Cuts at: 5280 5280
Size: 7690
KpnI G_GTAC'C
Cuts at: 5015 5015
Size: 7690
MfeI C'AATT_G
Cuts at: 5289 5289
Size: 7690
MluI A'CGCG_T
Cuts at: 7566 7566
Size: 7690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 3263 3576 6804 6988 1305
Size: 1332 626 313 3228 184 2007
Fragments arranged by size:
3228 2007 1332 626 313 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5868
Fragments arranged by size:
5868 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3051 3108 3129 4232 4917 5648
Size: 648 1011 874 57 21 1103 685 731
Cuts at: 5648 6007 6166 518
Size: 359 159 2042
Fragments arranged by size:
2042 1103 1011 874 731 685 648 359
159 57 21
MspA1I CmG'CkG
Cuts at: 1606 2673 3424 3487 3949 4345 4498 5106 5783
Size: 1067 751 63 462 396 153 608 677
Cuts at: 5783 6931 7176 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1067 751 677 608 462 396
245 153 63
NaeI GCC'GGC
Cuts at: 3444 3444
Size: 7690
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7690
NgoMIV G'CCGG_C
Cuts at: 3442 3442
Size: 7690
NheI G'CTAG_C
Cuts at: 1052 4219 1052
Size: 3167 4523
NotI GC'GGCC_GC
Cuts at: 5137 5137
Size: 7690
NsiI A_TGCA'T
Cuts at: 2805 2805
Size: 7690
NspI r_CATG'y
Cuts at: 1132 1229 2737 3128 5048 6593 1132
Size: 97 1508 391 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 1508 391 97
PasI CC'CwG_GG
Cuts at: 1348 2947 1348
Size: 1599 6091
PciI A'CATG_T
Cuts at: 6589 6589
Size: 7690
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2627 4774 1108
Size: 1519 2147 4024
Fragments arranged by size:
4024 2147 1519
PfoI T'CCnGG_A
Cuts at: 3867 3867
Size: 7690
PmeI GTTT'AAAC
Cuts at: 4994 4994
Size: 7690
PmlI CAC'GTG
Cuts at: 4315 4440 4315
Size: 125 7565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5759 5791 7272 7304 5759
Size: 32 1481 32 6145
Fragments arranged by size:
6145 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 2962 3871 4003 4186 4698 2746
Size: 216 909 132 183 512 5738
Fragments arranged by size:
5738 909 512 216 183 132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7690
PsiI TTA'TAA
Cuts at: 5260 5260
Size: 7690
PspOMI G'GGCC_C
Cuts at: 2513 4972 2513
Size: 2459 5231
PspXI vC'TCGA_Gb
Cuts at: 2142 2142
Size: 7690
PstI C_TGCA'G
Cuts at: 830 2609 3123 3957 4011 4862 5042 830
Size: 1779 514 834 54 851 180 3478
Fragments arranged by size:
3478 1779 851 834 514 180 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7690
PvuII CAG'CTG
Cuts at: 3424 3487 4345 3424
Size: 63 858 6769
Fragments arranged by size:
6769 858 63
SalI G'TCGA_C
Cuts at: 1936 5032 1936
Size: 3096 4594
SbfI CC_TGCA'GG
Cuts at: 5042 5042
Size: 7690
ScaI AGT'ACT
Cuts at: 4811 5938 7650 4811
Size: 1127 1712 4851
Fragments arranged by size:
4851 1712 1127
SfcI C'TryA_G
Cuts at: 826 1045 2605 3119 3953 4007 4858 5038 6173
Size: 219 1560 514 834 54 851 180 1135
Cuts at: 6173 6854 7045 826
Size: 681 191 1471
Fragments arranged by size:
1560 1471 1135 851 834 681 514 219
191 180 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7690
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7690
SmaI CCC'GGG
Cuts at: 1872 5017 1872
Size: 3145 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2142 5794 6695 6957 820
Size: 197 926 199 3652 901 262 1553
Fragments arranged by size:
3652 1553 926 901 262 199 197
SnaBI TAC'GTA
Cuts at: 493 5481 493
Size: 4988 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7690
SphI G_CATG'C
Cuts at: 1132 5048 1132
Size: 3916 3774
SspI AAT'ATT
Cuts at: 5 52 5614 5
Size: 47 5562 2081
Fragments arranged by size:
5562 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7690
StyI C'CwwG_G
Cuts at: 513 1065 2844 2871 4413 5129 513
Size: 552 1779 27 1542 716 3074
Fragments arranged by size:
3074 1779 1542 716 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3307 4766 5717 5734 5887 6072 6517
Size: 1255 1727 1459 951 17 153 185 445
Cuts at: 6517 325
Size: 1498
Fragments arranged by size:
1727 1498 1459 1255 951 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2297 2736 4809
Size: 274 80 33 51 1443 320 439 2073
Cuts at: 4809 5936 7648 96
Size: 1127 1712 138
Fragments arranged by size:
2073 1712 1443 1127 439 320 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2023 2055 2690 2722 3533 3565 7650 7682 2023
Size: 32 635 32 811 32 4085 32 2031
Fragments arranged by size:
4085 2031 811 635 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3032 3571 4037 1316
Size: 1716 539 466 4969
Fragments arranged by size:
4969 1716 539 466
XbaI T'CTAG_A
Cuts at: 4618 5026 4618
Size: 408 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 3979 4684 4775 2570
Size: 1409 705 91 5485
Fragments arranged by size:
5485 1409 705 91
XhoI C'TCGA_G
Cuts at: 1943 2142 1943
Size: 199 7491
XmaI C'CCGG_G
Cuts at: 1870 5015 1870
Size: 3145 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3860 5819 3416
Size: 444 1959 5287
Fragments arranged by size:
5287 1959 444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4036 5498 276
Size: 53 83 186 936 2502 1462 2468
Fragments arranged by size:
2502 2468 1462 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I EcoICRI
FspAI KasI NarI NruI PacI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI