(Linear) (Six-base) MAPSORT of: KIEE2563.seq Check: 6749 from: 1 to: 2985 KIEE2563.orf With 182 enzymes: SgfI * March 4, 2015 11:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 89 603 2985 Size: 89 514 2382 Fragments arranged by size: 2382 514 89 AatII G_ACGT'C Cuts at: 0 2033 2985 Size: 2033 952 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 2515 2985 Size: 709 1806 470 Fragments arranged by size: 1806 709 470 AfeI AGC'GCT Cuts at: 0 1498 2985 Size: 1498 1487 AgeI A'CCGG_T Cuts at: 0 928 2985 Size: 928 2057 AlwNI CAG_nnn'CTG Cuts at: 0 1671 1715 1947 2696 2769 2985 Size: 1671 44 232 749 73 216 Fragments arranged by size: 1671 749 232 216 73 44 ApaI G_GGCC'C Cuts at: 0 512 2971 2985 Size: 512 2459 14 Fragments arranged by size: 2459 512 14 ApoI r'AATT_y Cuts at: 0 582 802 819 2985 Size: 582 220 17 2166 Fragments arranged by size: 2166 582 220 17 AvaI C'yCGr_G Cuts at: 0 137 2985 Size: 137 2848 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1181 1214 2985 Size: 1181 33 1771 Fragments arranged by size: 1771 1181 33 BanI G'GyrC_C Cuts at: 0 964 2985 Size: 964 2021 BanII G_rGCy'C Cuts at: 0 459 512 614 977 1133 2971 2985 Size: 459 53 102 363 156 1838 14 Fragments arranged by size: 1838 459 363 156 102 53 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 1823 2985 Size: 1823 1162 BbvCI CC'TCA_GC Cuts at: 0 2963 2985 Size: 2963 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1701 1735 2985 Size: 1701 34 1250 Fragments arranged by size: 1701 1250 34 BclI T'GATC_A Cuts at: 0 500 2225 2985 Size: 500 1725 760 Fragments arranged by size: 1725 760 500 BfrBI ATG'CAT Cuts at: 0 798 2985 Size: 798 2187 BglI GCCn_nnn'nGGC Cuts at: 0 281 1392 1960 2985 Size: 281 1111 568 1025 Fragments arranged by size: 1111 1025 568 281 BglII A'GATC_T Cuts at: 0 54 573 1062 2457 2630 2985 Size: 54 519 489 1395 173 355 Fragments arranged by size: 1395 519 489 355 173 54 BlpI GC'TnA_GC Cuts at: 0 1483 2985 Size: 1483 1502 Bme1580I G_kGCm'C Cuts at: 0 512 2971 2985 Size: 512 2459 14 Fragments arranged by size: 2459 512 14 BmgBI CAC'GTC Cuts at: 0 1650 2985 Size: 1650 1335 BmrI ACTGGGnnnn_n' Cuts at: 0 1283 2171 2268 2985 Size: 1283 888 97 717 Fragments arranged by size: 1283 888 717 97 BmtI G_CTAG'C Cuts at: 0 2218 2985 Size: 2218 767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 249 379 1554 2985 Size: 249 130 1175 1431 Fragments arranged by size: 1431 1175 249 130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 1477 2362 2380 2424 2985 Size: 562 915 885 18 44 561 Fragments arranged by size: 915 885 562 561 44 18 Bpu10I CC'TnA_GC Cuts at: 0 214 1874 2207 2963 2985 Size: 214 1660 333 756 22 Fragments arranged by size: 1660 756 333 214 22 BsaI GGTCTCn'nnnn_ Cuts at: 0 110 1897 2985 Size: 110 1787 1088 Fragments arranged by size: 1787 1088 110 BsaAI yAC'GTr Cuts at: 0 2310 2435 2985 Size: 2310 125 550 Fragments arranged by size: 2310 550 125 BsaHI Gr'CG_yC Cuts at: 0 988 2030 2062 2985 Size: 988 1042 32 923 Fragments arranged by size: 1042 988 923 32 BsaWI w'CCGG_w Cuts at: 0 928 2985 Size: 928 2057 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 517 547 915 945 2985 Size: 517 30 368 30 2040 Fragments arranged by size: 2040 517 368 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 155 2359 2985 Size: 155 2204 626 Fragments arranged by size: 2204 626 155 BseYI C'CCAG_C Cuts at: 0 1443 2762 2985 Size: 1443 1319 223 Fragments arranged by size: 1443 1319 223 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1978 2611 2936 2985 Size: 1978 633 325 49 Fragments arranged by size: 1978 633 325 49 BsiEI CG_ry'CG Cuts at: 0 1438 2985 Size: 1438 1547 BsiHKAI G_wGCw'C Cuts at: 0 659 679 2440 2985 Size: 659 20 1761 545 Fragments arranged by size: 1761 659 545 20 BsmI GAATG_Cn' Cuts at: 0 2239 2985 Size: 2239 746 Bsp1286I G_dGCh'C Cuts at: 0 459 512 614 659 679 977 1133 2440 Size: 459 53 102 45 20 298 156 1307 Cuts at: 2440 2971 2985 Size: 531 14 Fragments arranged by size: 1307 531 459 298 156 102 53 45 20 14 BspHI T'CATG_A Cuts at: 0 1185 1583 2985 Size: 1185 398 1402 Fragments arranged by size: 1402 1185 398 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 89 603 945 2985 Size: 89 514 342 2040 Fragments arranged by size: 2040 514 342 89 BsrDI GCAATG_nn' Cuts at: 0 915 2957 2985 Size: 915 2042 28 Fragments arranged by size: 2042 915 28 BsrFI r'CCGG_y Cuts at: 0 928 1437 2985 Size: 928 509 1548 Fragments arranged by size: 1548 928 509 BsrGI T'GTAC_A Cuts at: 0 292 2985 Size: 292 2693 BssSI C'ACGA_G Cuts at: 0 107 399 706 2135 2260 2985 Size: 107 292 307 1429 125 725 Fragments arranged by size: 1429 725 307 292 125 107 BstAPI GCAn_nnn'nTGC Cuts at: 0 683 1715 1947 1956 2926 2985 Size: 683 1032 232 9 970 59 Fragments arranged by size: 1032 970 683 232 59 9 BstEII G'GTnAC_C Cuts at: 0 155 2985 Size: 155 2830 BstXI CCAn_nnnn'nTGG Cuts at: 0 1296 2073 2985 Size: 1296 777 912 Fragments arranged by size: 1296 912 777 BstYI r'GATC_y Cuts at: 0 54 573 1062 2303 2457 2630 2985 Size: 54 519 489 1241 154 173 355 Fragments arranged by size: 1241 519 489 355 173 154 54 Bsu36I CC'TnA_GG Cuts at: 0 224 2985 Size: 224 2761 BtgI C'CryG_G Cuts at: 0 459 2744 2985 Size: 459 2285 241 Fragments arranged by size: 2285 459 241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1433 2985 Size: 1433 1552 BtsI GCAGTG_nn' Cuts at: 0 198 258 556 766 1504 2084 2599 2792 Size: 198 60 298 210 738 580 515 193 Cuts at: 2792 2985 Size: 193 Fragments arranged by size: 738 580 515 298 210 198 193 193 60 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 615 650 2246 2281 2985 Size: 615 35 1596 35 704 Fragments arranged by size: 1596 704 615 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1291 2985 Size: 1291 1694 DrdI GACnn_nn'nnGTC Cuts at: 0 304 1621 2985 Size: 304 1317 1364 Fragments arranged by size: 1364 1317 304 EaeI y'GGCC_r Cuts at: 0 1435 1472 1520 1979 2985 Size: 1435 37 48 459 1006 Fragments arranged by size: 1435 1006 459 48 37 EagI C'GGCC_G Cuts at: 0 1435 2985 Size: 1435 1550 EarI CTCTTCn'nnn_ Cuts at: 0 1582 2042 2985 Size: 1582 460 943 Fragments arranged by size: 1582 943 460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 498 1501 1855 2985 Size: 498 1003 354 1130 Fragments arranged by size: 1130 1003 498 354 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 709 1477 2362 2380 2424 2515 2985 Size: 562 147 768 885 18 44 91 470 Fragments arranged by size: 885 768 562 470 147 91 44 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 1723 2985 Size: 1723 1262 EcoO109I rG'GnC_Cy Cuts at: 0 741 957 1370 1866 1998 2181 2391 2693 Size: 741 216 413 496 132 183 210 302 Cuts at: 2693 2967 2985 Size: 274 18 Fragments arranged by size: 741 496 413 302 274 216 210 183 132 18 EcoRI G'AATT_C Cuts at: 0 582 2985 Size: 582 2403 EcoRV GAT'ATC Cuts at: 0 1702 2985 Size: 1702 1283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1105 1641 2985 Size: 1105 536 1344 Fragments arranged by size: 1344 1105 536 FseI GG_CCGG'CC Cuts at: 0 1441 2985 Size: 1441 1544 HaeII r_GCGC'y Cuts at: 0 1500 2568 2985 Size: 1500 1068 417 Fragments arranged by size: 1500 1068 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 941 973 1001 1033 1138 1170 Size: 517 32 392 32 28 32 105 32 Cuts at: 1170 1297 1329 2985 Size: 127 32 1656 Fragments arranged by size: 1656 517 392 127 105 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 0 1809 2037 2985 Size: 1809 228 948 Fragments arranged by size: 1809 948 228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 632 1258 1571 2985 Size: 632 626 313 1414 Fragments arranged by size: 1414 632 626 313 MslI CAynn'nnrTG Cuts at: 0 172 1046 1103 1124 2227 2912 2985 Size: 172 874 57 21 1103 685 73 Fragments arranged by size: 1103 874 685 172 73 57 21 MspA1I CmG'CkG Cuts at: 0 668 1419 1482 1944 2340 2493 2985 Size: 668 751 63 462 396 153 492 Fragments arranged by size: 751 668 492 462 396 153 63 NaeI GCC'GGC Cuts at: 0 1439 2985 Size: 1439 1546 NgoMIV G'CCGG_C Cuts at: 0 1437 2985 Size: 1437 1548 NheI G'CTAG_C Cuts at: 0 2214 2985 Size: 2214 771 NsiI A_TGCA'T Cuts at: 0 800 2985 Size: 800 2185 NspI r_CATG'y Cuts at: 0 732 1123 2985 Size: 732 391 1862 Fragments arranged by size: 1862 732 391 PasI CC'CwG_GG Cuts at: 0 942 2985 Size: 942 2043 PflMI CCAn_nnn'nTGG Cuts at: 0 622 2769 2985 Size: 622 2147 216 Fragments arranged by size: 2147 622 216 PfoI T'CCnGG_A Cuts at: 0 1862 2985 Size: 1862 1123 PmlI CAC'GTG Cuts at: 0 2310 2435 2985 Size: 2310 125 550 Fragments arranged by size: 2310 550 125 PpuMI rG'GwC_Cy Cuts at: 0 741 957 1866 1998 2181 2693 2985 Size: 741 216 909 132 183 512 292 Fragments arranged by size: 909 741 512 292 216 183 132 PspOMI G'GGCC_C Cuts at: 0 508 2967 2985 Size: 508 2459 18 Fragments arranged by size: 2459 508 18 PspXI vC'TCGA_Gb Cuts at: 0 137 2985 Size: 137 2848 PstI C_TGCA'G Cuts at: 0 604 1118 1952 2006 2857 2985 Size: 604 514 834 54 851 128 Fragments arranged by size: 851 834 604 514 128 54 PvuII CAG'CTG Cuts at: 0 1419 1482 2340 2985 Size: 1419 63 858 645 Fragments arranged by size: 1419 858 645 63 ScaI AGT'ACT Cuts at: 0 2806 2985 Size: 2806 179 SfcI C'TryA_G Cuts at: 0 600 1114 1948 2002 2853 2985 Size: 600 514 834 54 851 132 Fragments arranged by size: 851 834 600 514 132 54 SmlI C'TyrA_G Cuts at: 0 137 2985 Size: 137 2848 StyI C'CwwG_G Cuts at: 0 839 866 2408 2985 Size: 839 27 1542 577 Fragments arranged by size: 1542 839 577 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1302 2761 2985 Size: 1302 1459 224 Fragments arranged by size: 1459 1302 224 TatI w'GTAC_w Cuts at: 0 292 731 2804 2985 Size: 292 439 2073 181 Fragments arranged by size: 2073 439 292 181 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 18 50 685 717 1528 1560 2985 Size: 18 32 635 32 811 32 1425 Fragments arranged by size: 1425 811 635 32 32 32 18 Tth111I GACn'n_nGTC Cuts at: 0 1027 1566 2032 2985 Size: 1027 539 466 953 Fragments arranged by size: 1027 953 539 466 XbaI T'CTAG_A Cuts at: 0 2613 2985 Size: 2613 372 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 565 1974 2679 2770 2985 Size: 565 1409 705 91 215 Fragments arranged by size: 1409 705 565 215 91 XhoI C'TCGA_G Cuts at: 0 137 2985 Size: 137 2848 XmnI GAAnn'nnTTC Cuts at: 0 1411 1855 2985 Size: 1411 444 1130 Fragments arranged by size: 1411 1130 444 ZraI GAC'GTC Cuts at: 0 2031 2985 Size: 2031 954 Enzymes that do cut: AarI AatII AcuI AfeI AgeI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII ScaI SfcI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BciVI BpuEI BsaBI BsiWI BsmBI BspEI BsrBI BssHII BstBI BstZ17I ClaI DraI EcoICRI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XmaI