Restriction map for
pFN21AB9284
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 475 GGTACC G^GTACC
AflIII 119 ACATGT A^C[GA][CT]GT
AlwI 22 GGATC GGATC(4/5)
ApaI 542 GGGCCC GGGCC^C
Asp718I 475 GGTACC G^GTACC
AvaII 452 GGACC G^G[AT]CC
BalI 461 TGGCCA TGG^CCA
BanI 475 GGTACC G^G[CT][GA]CC
BciVI 123 GTATCC GTATCC(6/5)
BfaI 416 CTAG C^TAG
BpmI 128 CTGGAG CTGGAG(16/14)
BsgI 364 GTGCAG GTGCAG(16/14)
BsmFI 451 GGGAC GGGAC(10/14)
BspLU11I 119 ACATGT A^CATGT
BspMI 472 GCAGGT ACCTGC(4/8)
BstAPI 97 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 429 GGTCACC G^GT[ACGT]ACC
BstPI 429 GGTCACC G^GT[ACGT]ACC
BstXI 434 CCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 22 GGATCT [GA]^GATC[CT]
DdeI 578 CTCAG C^T[ACGT]AG
DpnII 23 GATC ^GATC
DraII 542 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 461 TGGCCA [CT]^GGCC[GA]
EcoNI 412 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 542 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 429 GGTCACC G^GT[ACGT]ACC
FauI 200 GCGGG CCCGC(4/6)
HphI 431 TCACC GGTGA(8/7)
KpnI 475 GGTACC GGTAC^C
MaeI 416 CTAG C^TAG
MboI 23 GATC ^GATC
MboII 25 TCTTC GAAGA(8/7)
MflI 22 GGATCT [GA]^GATC[CT]
MluNI 461 TGGCCA TGG^CCA
MscI 461 TGGCCA TGG^CCA
NdeII 23 GATC ^GATC
PvuII 68 CAGCTG CAG^CTG
Sau3AI 23 GATC ^GATC
SphI 377 GCATGC GCATG^C
Tsp509I 57 AATT ^AATT
XcmI 434 CCATGGGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 22 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 319 CCGC CCGC(-3/-1)
200 GCGG
AlwNI 98 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
316 CAGCCGCTG
BanII 282 GAGCCC G[GA]GC[CT]^C
542 GGGCCC
BcnI 519 CCCGG CC^[GC]GG
540 CCGGG
BsaAI 303 CACGTA [CT]AC^GT[GA]
534 CACGTA
BstF5I 345 CATCC GGATG(2/0)
405 CATCC
Cac8I 377 GCATGC GC[ACGT]^[ACGT]GC
381 GCCTGC
FokI 345 CATCC GGATG(9/13)
405 CATCC
HaeIII 462 GGCC GG^CC
543 GGCC
HapII 520 CCGG C^CGG
540 CCGG
HinfI 3 GAATC G^A[ACGT]TC
135 GAATC
HpaII 520 CCGG C^CGG
540 CCGG
MspI 520 CCGG C^CGG
540 CCGG
NciI 519 CCCGG CC^[GC]GG
540 CCGGG
NspI 119 ACATGT [GA]CATG^[CT]
377 GCATGC
TfiI 3 GAATC G^A[AT]TC
135 GAATC
Tsp45I 430 GTCAC ^GT[GC]AC
523 GTCAC
TspRI 525 CACTG CA[GC]TG[ACGT][ACGT]^
580 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI Cfr13I DsaI EcoRII EcoT14I
Fnu4HI ItaI MaeII MaeIII MnlI
MslI MspA1I MvaI MwoI NcoI
NlaIII NlaIV RsaI Sau96I ScrFI
StyI TseI