Restriction map for
pFN21AB9284
(Linear) (Six-base) MAPSORT of: KIBB9284.seq Check: 1119 from: 1 to: 585
KIBB9284.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
Acc65I G'GTAC_C
Cuts at: 0 475 585
Size: 475 110
AflIII A'CryG_T
Cuts at: 0 119 585
Size: 119 466
AleI CACnn'nnGTG
Cuts at: 0 358 361 585
Size: 358 3 224
Fragments arranged by size:
358 224 3
AlwNI CAG_nnn'CTG
Cuts at: 0 103 321 585
Size: 103 218 264
Fragments arranged by size:
264 218 103
ApaI G_GGCC'C
Cuts at: 0 546 585
Size: 546 39
BanI G'GyrC_C
Cuts at: 0 475 585
Size: 475 110
BanII G_rGCy'C
Cuts at: 0 286 546 585
Size: 286 260 39
Fragments arranged by size:
286 260 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 134 585
Size: 134 451
Bme1580I G_kGCm'C
Cuts at: 0 533 546 575 585
Size: 533 13 29 10
Fragments arranged by size:
533 29 13 10
BmgBI CAC'GTC
Cuts at: 0 342 585
Size: 342 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 585
Size: 149 436
BsaAI yAC'GTr
Cuts at: 0 305 536 585
Size: 305 231 49
Fragments arranged by size:
305 231 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 585
Size: 208 30 347
Fragments arranged by size:
347 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 213 326 585
Size: 210 3 113 259
Fragments arranged by size:
259 210 113 3
BseYI C'CCAG_C
Cuts at: 0 275 448 585
Size: 275 173 137
Fragments arranged by size:
275 173 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 585
Size: 385 200
Bsp1286I G_dGCh'C
Cuts at: 0 286 533 546 575 585
Size: 286 247 13 29 10
Fragments arranged by size:
286 247 29 13 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 463 585
Size: 463 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 103 585
Size: 103 482
BstEII G'GTnAC_C
Cuts at: 0 429 585
Size: 429 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 585
Size: 441 144
BstYI r'GATC_y
Cuts at: 0 22 585
Size: 22 563
BtgI C'CryG_G
Cuts at: 0 80 356 434 458 585
Size: 80 276 78 24 127
Fragments arranged by size:
276 127 80 78 24
EaeI y'GGCC_r
Cuts at: 0 461 585
Size: 461 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 585
Size: 149 436
EcoNI CCTnn'n_nnAGG
Cuts at: 0 416 585
Size: 416 169
EcoO109I rG'GnC_Cy
Cuts at: 0 543 585
Size: 543 42
KpnI G_GTAC'C
Cuts at: 0 479 585
Size: 479 106
MscI TGG'CCA
Cuts at: 0 463 585
Size: 463 122
MslI CAynn'nnrTG
Cuts at: 0 358 361 439 463 585
Size: 358 3 78 24 122
Fragments arranged by size:
358 122 78 24 3
MspA1I CmG'CkG
Cuts at: 0 70 200 321 585
Size: 70 130 121 264
Fragments arranged by size:
264 130 121 70
NcoI C'CATG_G
Cuts at: 0 80 434 458 585
Size: 80 354 24 127
Fragments arranged by size:
354 127 80 24
NspI r_CATG'y
Cuts at: 0 123 381 585
Size: 123 258 204
Fragments arranged by size:
258 204 123
PciI A'CATG_T
Cuts at: 0 119 585
Size: 119 466
PfoI T'CCnGG_A
Cuts at: 0 18 126 585
Size: 18 108 459
Fragments arranged by size:
459 108 18
PspOMI G'GGCC_C
Cuts at: 0 542 585
Size: 542 43
PvuII CAG'CTG
Cuts at: 0 70 585
Size: 70 515
SphI G_CATG'C
Cuts at: 0 381 585
Size: 381 204
StyI C'CwwG_G
Cuts at: 0 80 434 458 585
Size: 80 354 24 127
Fragments arranged by size:
354 127 80 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 585
Size: 441 144
Enzymes that do cut:
Acc65I AflIII AleI AlwNI ApaI BanI BanII BciVI
Bme1580I BmgBI BpmI BsaAI BsaXI BseRI BseYI BsgI
Bsp1286I BspMI BstAPI BstEII BstXI BstYI BtgI EaeI
Eco57MI EcoNI EcoO109I KpnI MscI MslI MspA1I NcoI
NspI PciI PfoI PspOMI PvuII SphI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI