Restriction map for
pFN21AB9044
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MboI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI StyI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 1148 GCGG CCGC(-3/-1)
AgeI 1499 ACCGGT A^CCGGT
AspHI 1251 GTGCTC G[AT]GC[AT]^C
BanII 208 GAGCCC G[GA]GC[CT]^C
BsaAI 475 TACGTG [CT]AC^GT[GA]
BsaWI 1499 ACCGGT [AT]^CCGG[AT]
BseRI 73 GAGGAG GAGGAG(10/8)
BsgI 95 CTGCAC GTGCAG(16/14)
BsiHKAI 1251 GTGCTC G[AT]GC[AT]^C
BsmFI 30 GGGAC GGGAC(10/14)
BsmI 1144 GAATGC GAATGC(1/-1)
BsrFI 1499 ACCGGT [GA]^CCGG[CT]
BstAPI 1423 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1499 ACCGGT [GA]^CCGG[CT]
EcoNI 212 CCTGGAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 1148 GCGGC GC^[ACGT]GC
HapII 1500 CCGG C^CGG
HpaII 1500 CCGG C^CGG
ItaI 1148 GCGGC GC^[ACGT]GC
MaeII 476 ACGT A^CGT
MaeIII 896 GTCAC ^GT[ACGT]AC
MslI 1534 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1500 CCGG C^CGG
MwoI 1423 GCAACTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 30 GGGACC GG[ACGT]^[ACGT]CC
NspI 575 GCATGC [GA]CATG^[CT]
PinAI 1499 ACCGGT A^CCGGT
PleI 391 GAGTC GAGTC(4/5)
SexAI 143 ACCTGGT A^CC[AT]GGT
SfcI 135 CTACAG C^T[GA][CT]AG
SphI 575 GCATGC GCATG^C
SspI 456 AATATT AAT^ATT
TaqI 1266 TCGA T^CGA
TfiI 304 GATTC G^A[AT]TC
Tsp45I 896 GTCAC ^GT[GC]AC
TthHB8I 1266 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 53 TGGCCA TGG^CCA
283 TGGCCA
BbsI 274 GAAGAC GAAGAC(2/6)
148 GTCTTC
BmyI 208 GAGCCC G[AGT]GC[ACT]^C
1251 GTGCTC
BpuAI 274 GAAGAC GAAGAC(2/6)
148 GTCTTC
BsaJI 153 CCTGGG C^C[ACGT][ACGT]GG
211 CCCTGG
Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C
1251 GTGCTC
BsrDI 81 GCAATG GCAATG(2/0)
1403 GCAATG
BstF5I 48 GGATG GGATG(2/0)
387 GGATG
Cac8I 575 GCATGC GC[ACGT]^[ACGT]GC
1419 GCCTGC
EaeI 53 TGGCCA [CT]^GGCC[GA]
283 TGGCCA
EarI 222 GAAGAG CTCTTC(1/4)
784 GAAGAG
FokI 48 GGATG GGATG(9/13)
387 GGATG
HinfI 304 GATTC G^A[ACGT]TC
391 GAGTC
HphI 1540 GGTGA GGTGA(8/7)
177 TCACC
Ksp632I 222 GAAGAG CTCTTC(1/4)
784 GAAGAG
MluNI 53 TGGCCA TGG^CCA
283 TGGCCA
MscI 53 TGGCCA TGG^CCA
283 TGGCCA
NdeI 694 CATATG CA^TATG
1186 CATATG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI Asp700I AvaII
BfaI BpmI BsiYI BslI BsmAI
BsrI BssKI BstNI Cfr13I DdeI
EcoRI EcoRII HaeIII HindIII MaeI
MboII MnlI MseI MvaI NlaIII
Sau96I ScrFI Tru9I Tsp509I TspRI
XmnI