(Circular) (Six-base) MAPSORT of: pFN21AB9044.seq Check: 8891 from: 1 to: 6295 pFN21AB9044 6295 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2298 4358 5742 6010 1632 Size: 666 2060 1384 268 1917 Fragments arranged by size: 2060 1917 1384 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 3504 5126 3504 Size: 1622 4673 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 4038 5610 4038 Size: 1572 4723 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2066 2110 2359 2498 2506 2610 2626 2694 Size: 573 44 249 139 8 104 16 68 Cuts at: 2694 2778 2946 3030 3114 3198 3282 3450 3534 Size: 84 168 84 84 84 84 168 84 Cuts at: 3534 3604 3830 3982 6091 1493 Size: 70 226 152 2109 1697 Fragments arranged by size: 2109 1697 573 249 226 168 168 152 139 104 84 84 84 84 84 84 70 68 44 16 8 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 3620 1870 Size: 73 1677 4545 Fragments arranged by size: 4545 1677 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3616 5071 618 Size: 325 217 627 1829 1455 1842 Fragments arranged by size: 1842 1829 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 3614 1391 Size: 826 1397 4072 Fragments arranged by size: 4072 1397 826 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 2286 928 Size: 971 247 140 4937 Fragments arranged by size: 4937 971 247 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4486 4520 1903 Size: 34 50 34 2465 34 3678 Fragments arranged by size: 3678 2465 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 4356 5512 1790 Size: 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2249 4026 4984 Size: 416 53 83 399 24 832 1777 958 Cuts at: 4984 442 Size: 1753 Fragments arranged by size: 1777 1753 958 832 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2892 2976 3060 3144 3228 3244 3396 Size: 857 796 84 84 84 84 16 152 Cuts at: 3396 4955 1239 Size: 1559 2579 Fragments arranged by size: 2579 1559 857 796 152 84 84 84 84 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4420 5285 5583 4420 Size: 865 298 5132 Fragments arranged by size: 5132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2482 4086 493 Size: 711 1278 1604 2702 Fragments arranged by size: 2702 1604 1278 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3504 4728 5126 5400 5547 5958 3504 Size: 1224 398 274 147 411 3841 Fragments arranged by size: 3841 1224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 1186 Size: 907 5388 BseYI C'CCAG_C Cuts at: 5498 5994 5498 Size: 496 5799 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2085 1163 Size: 573 349 5373 Fragments arranged by size: 5373 573 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3260 4356 4441 5512 3260 Size: 1096 85 1071 4043 Fragments arranged by size: 4043 1096 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3806 3899 3155 Size: 651 93 5551 Fragments arranged by size: 5551 651 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 3260 3614 4356 4441 5512 1391 Size: 399 427 1043 354 742 85 1071 2174 Fragments arranged by size: 2174 1071 1043 742 427 399 354 85 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3650 844 Size: 2806 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2093 3415 4790 4964 65 Size: 1183 845 1322 1375 174 1396 Fragments arranged by size: 1396 1375 1322 1183 845 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3504 4939 5126 1700 Size: 110 1694 1435 187 2869 Fragments arranged by size: 2869 1694 1435 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 3434 794 Size: 2640 3655 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstYI r'GATC_y Cuts at: 1972 3625 3992 4377 4394 5846 5932 5944 6290 Size: 1653 367 385 17 1452 86 12 346 Cuts at: 6290 1972 Size: 1977 Fragments arranged by size: 1977 1653 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5743 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 2097 3811 4605 4625 2097 Size: 1714 794 20 3767 Fragments arranged by size: 3767 1714 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2288 3742 Size: 54 1425 50 275 18 228 230 1454 Cuts at: 3742 4631 6260 8 Size: 889 1629 43 Fragments arranged by size: 1629 1454 1425 889 275 230 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2784 4225 1353 Size: 869 562 1441 3423 Fragments arranged by size: 3423 1441 869 562 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2298 2892 2976 3060 3144 3228 Size: 393 464 202 594 84 84 84 84 Cuts at: 3228 3244 3396 4358 4955 5742 6010 1239 Size: 16 152 962 597 787 268 1524 Fragments arranged by size: 1524 962 787 597 594 464 393 268 202 152 84 84 84 84 16 EcoNI CCTnn'n_nnAGG Cuts at: 2221 2221 Size: 6295 EcoRI G'AATT_C Cuts at: 2066 2610 2626 2694 2946 3198 3282 3450 3604 Size: 544 16 68 252 252 84 168 154 Cuts at: 3604 3982 2066 Size: 378 4379 Fragments arranged by size: 4379 544 378 252 252 168 154 84 68 16 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373 Size: 32 135 32 3364 32 42 32 2626 Fragments arranged by size: 3364 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 3075 3159 3243 3495 748 Size: 2327 84 84 252 3548 Fragments arranged by size: 3548 2327 252 84 84 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 2290 10 Size: 54 1425 343 228 230 4015 Fragments arranged by size: 4015 1425 343 230 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 3543 4253 4612 4771 518 Size: 648 2377 710 359 159 2042 Fragments arranged by size: 2377 2042 710 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3711 4388 5536 5781 1606 Size: 2105 677 1148 245 2120 Fragments arranged by size: 2120 2105 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5743 NdeI CA'TA_TG Cuts at: 387 2700 3192 387 Size: 2313 492 3490 Fragments arranged by size: 3490 2313 492 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2584 3653 5198 1132 Size: 97 1355 1069 1545 2229 Fragments arranged by size: 2229 1545 1355 1069 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 2421 3865 2421 Size: 1444 4851 PstI C_TGCA'G Cuts at: 830 3647 830 Size: 2817 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SexAI A'CCwGG_T Cuts at: 2148 2148 Size: 6295 SfcI C'TryA_G Cuts at: 826 1045 2140 3643 4778 5459 5650 826 Size: 219 1095 1503 1135 681 191 1471 Fragments arranged by size: 1503 1471 1135 1095 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4399 5300 5562 820 Size: 197 926 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 2584 3653 1132 Size: 1452 1069 3774 Fragments arranged by size: 3774 1452 1069 SspI AAT'ATT Cuts at: 5 52 2463 4219 5 Size: 47 2411 1756 2081 Fragments arranged by size: 2411 2081 1756 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 3734 513 Size: 552 2669 3074 Fragments arranged by size: 3074 2669 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3243 4322 4339 4492 4677 5122 325 Size: 1255 1663 1079 17 153 185 445 1498 Fragments arranged by size: 1663 1498 1255 1079 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4541 6253 96 Size: 274 80 33 51 1443 2564 1712 138 Fragments arranged by size: 2564 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6255 6287 6255 Size: 32 6263 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 2546 2798 2966 4424 2546 Size: 252 168 1458 4417 Fragments arranged by size: 4417 1458 252 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI