################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AlwI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MunI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI Sau3AI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 559 GTCTAC GT^[AC][GT]AC AcsI 1460 GAATTC [GA]^AATT[CT] Alw44I 508 GTGCAC G^TGCAC AlwNI 171 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 35 AGCGCT AGC^GCT ApaLI 508 GTGCAC G^TGCAC ApoI 1460 GAATTC [GA]^AATT[CT] AscI 490 GGCGCGCC GG^CGCGCC Asp700I 33 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 812 TGGCCA TGG^CCA BsaI 1063 GAGACC GGTCTC(1/5) BsaWI 1755 ACCGGA [AT]^CCGG[AT] BsmBI 774 CGTCTC CGTCTC(1/5) BsrDI 1394 GCAATG GCAATG(2/0) BstXI 572 CCACCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eco47III 35 AGCGCT AGC^GCT EcoNI 1137 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1460 GAATTC G^AATTC MluNI 812 TGGCCA TGG^CCA MscI 812 TGGCCA TGG^CCA PflMI 1355 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1675 GAGCTC GAGCT^C SbfI 612 CCTGCAGG CCTGCA^GG SfaNI 255 GCATC GCATC(5/9) SmlI 1678 CTCAAG C^T[CT][GA]AG Sse8387I 612 CCTGCAGG CCTGCA^GG StuI 680 AGGCCT AGG^CCT TfiI 1759 GATTC G^A[AT]TC Van91I 1355 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 33 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AocI 676 CCTCAGG CC^T[ACGT]AGG 1613 CCTCAGG ApaI 156 GGGCCC GGGCC^C 300 GGGCCC BbvCI 187 CCTCAGC CCTCAGC(-5/-2) 1266 CCTCAGC BlpI 405 GCTGAGC GC^T[ACGT]AGC 758 GCTCAGC Bpu1102I 405 GCTGAGC GC^T[ACGT]AGC 758 GCTCAGC BsaAI 562 TACGTG [CT]AC^GT[GA] 1018 TACGTG BseRI 129 CTCCTC GAGGAG(10/8) 433 GAGGAG BsgI 1075 CTGCAC GTGCAG(16/14) 1130 GTGCAG BspMI 611 ACCTGC ACCTGC(4/8) 1136 ACCTGC BstAPI 1077 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1232 GCAACGCCTGC Bsu36I 676 CCTCAGG CC^T[ACGT]AGG 1613 CCTCAGG CelII 405 GCTGAGC GC^T[ACGT]AGC 758 GCTCAGC EarI 117 CTCTTC CTCTTC(1/4) 1557 CTCTTC Eco81I 676 CCTCAGG CC^T[ACGT]AGG 1613 CCTCAGG HphI 1381 GGTGA GGTGA(8/7) 1632 GGTGA Ksp632I 117 CTCTTC CTCTTC(1/4) 1557 CTCTTC MaeIII 1340 GTCAC ^GT[ACGT]AC 1738 GTCAC MboII 118 TCTTC GAAGA(8/7) 1558 TCTTC MslI 573 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1533 CATTCCCATG NaeI 1271 GCCGGC GCC^GGC 1728 GCCGGC NgoMIV 1271 GCCGGC G^CCGGC 1728 GCCGGC PpuMI 1122 GGGTCCC [GA]G^G[AT]CC[CT] 1145 AGGACCT PstI 613 CTGCAG CTGCA^G 1084 CTGCAG Tsp45I 1340 GTCAC ^GT[GC]AC 1738 GTCAC Tsp509I 1461 AATT ^AATT 1528 AATT XcmI 569 CCACCACCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 800 CCAGCCCGCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NlaIII NlaIV NotI PleI RsaI SacII Sau96I ScrFI SfcI SfoI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI