(Circular) (Six-base) MAPSORT of: pFN21AB9437.seq Check: 3012 from: 1 to: 6490 pFN21AB9437 6490 bp With 182 enzymes: SgfI * April 28, 2014 11:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2625 2625 Size: 6490 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 3833 1937 Size: 628 1268 4594 Fragments arranged by size: 4594 1268 628 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4553 5937 6205 1632 Size: 2921 1384 268 1917 Fragments arranged by size: 2921 1917 1384 268 AfeI AGC'GCT Cuts at: 2042 2042 Size: 6490 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 5389 6366 1203 Size: 4186 977 1327 Fragments arranged by size: 4186 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 2582 1166 Size: 1416 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3170 3202 3170 Size: 32 6458 AlwNI CAG_nnn'CTG Cuts at: 2181 4233 5805 2181 Size: 2052 1572 2866 Fragments arranged by size: 2866 2052 1572 ApaI G_GGCC'C Cuts at: 2165 2309 2165 Size: 144 6346 ApaLI G'TGCA_C Cuts at: 2513 4547 5703 2513 Size: 2034 1156 3300 Fragments arranged by size: 3300 2034 1156 ApoI r'AATT_y Cuts at: 1493 3465 3799 4025 4177 6286 1493 Size: 1972 334 226 152 2109 1697 Fragments arranged by size: 2109 1972 1697 334 226 152 AscI GG'CGCG_CC Cuts at: 2496 2496 Size: 6490 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2309 2426 3123 3668 3815 1870 Size: 73 202 164 117 697 545 147 4545 Fragments arranged by size: 4545 697 545 202 164 147 117 73 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 2290 2402 2825 3031 Size: 325 217 627 384 119 112 423 206 Cuts at: 3031 3118 3218 3433 3629 3811 5266 618 Size: 87 100 215 196 182 1455 1842 Fragments arranged by size: 1842 1455 627 423 384 325 217 215 206 196 182 119 112 100 87 BanII G_rGCy'C Cuts at: 1391 2100 2165 2275 2309 2417 2928 3011 3109 Size: 709 65 110 34 108 511 83 98 Cuts at: 3109 3252 3684 3708 3809 1391 Size: 143 432 24 101 4072 Fragments arranged by size: 4072 709 511 432 143 110 108 101 98 83 65 34 24 BbeI G_GCGC'C Cuts at: 2175 2294 2406 2829 3035 2175 Size: 119 112 423 206 5630 Fragments arranged by size: 5630 423 206 119 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5519 BbvCI CC'TCA_GC Cuts at: 2193 3272 2193 Size: 1079 5411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 2313 2531 Size: 107 122 71 631 605 497 144 218 Cuts at: 2531 2546 3049 3205 3623 3734 5101 136 Size: 15 503 156 418 111 1367 1525 Fragments arranged by size: 1525 1367 631 605 503 497 418 218 156 144 122 111 107 71 15 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 2411 2764 3907 2411 Size: 353 1143 4994 Fragments arranged by size: 4994 1143 353 Bme1580I G_kGCm'C Cuts at: 1790 2165 2309 2517 2574 3123 3438 3634 4551 Size: 375 144 208 57 549 315 196 917 Cuts at: 4551 5707 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 917 549 375 315 208 196 144 57 BmgBI CAC'GTC Cuts at: 1318 2779 3747 1318 Size: 1461 968 4061 Fragments arranged by size: 4061 1461 968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2923 3126 4221 Size: 416 53 83 399 24 1506 203 1095 Cuts at: 4221 5179 442 Size: 958 1753 Fragments arranged by size: 1753 1506 1095 958 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3128 3128 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2452 3167 5150 1239 Size: 820 393 715 1983 2579 Fragments arranged by size: 2579 1983 820 715 393 Bpu10I CC'TnA_GC Cuts at: 2193 3272 2193 Size: 1079 5411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3668 4615 5480 5778 3668 Size: 947 865 298 4380 Fragments arranged by size: 4380 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 5153 882 Size: 617 1563 2091 2219 Fragments arranged by size: 2219 2091 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 3025 4281 493 Size: 711 1365 456 1256 2702 Fragments arranged by size: 2702 1365 1256 711 456 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2172 2189 2291 Size: 53 83 186 936 531 108 17 102 Cuts at: 2291 2403 2826 3032 3041 3197 3717 4297 4679 Size: 112 423 206 9 156 520 580 382 Cuts at: 4679 5314 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 580 531 520 423 382 206 186 156 112 108 102 83 53 17 9 BsaWI w'CCGG_w Cuts at: 3760 4923 5321 5595 5742 6153 3760 Size: 1163 398 274 147 411 4097 Fragments arranged by size: 4097 1163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 3140 3170 3200 Size: 30 165 30 1026 30 681 30 30 Cuts at: 3200 1178 Size: 4468 Fragments arranged by size: 4468 1026 681 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 2453 1186 Size: 939 328 5223 Fragments arranged by size: 5223 939 328 BseYI C'CCAG_C Cuts at: 2328 2804 5693 6189 2328 Size: 476 2889 496 2629 Fragments arranged by size: 2889 2629 496 476 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3065 3156 1163 Size: 573 1329 91 4497 Fragments arranged by size: 4497 1329 573 91 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2217 2373 2484 2526 2694 2920 Size: 275 186 216 156 111 42 168 226 Cuts at: 2920 2949 2957 3738 3761 3940 4701 5729 1540 Size: 29 8 781 23 179 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 275 226 216 186 179 168 156 111 42 29 23 8 BsiHKAI G_wGCw'C Cuts at: 2517 3662 3684 4551 4636 5707 2517 Size: 1145 22 867 85 1071 3300 Fragments arranged by size: 3300 1145 1071 867 85 22 BsmI GAATG_Cn' Cuts at: 4001 4094 4001 Size: 93 6397 BsmBI CGTCTCn'nnnn_ Cuts at: 2785 2785 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2165 2275 2309 2417 2517 2574 Size: 399 310 65 110 34 108 100 57 Cuts at: 2574 2928 3011 3109 3123 3252 3438 3634 3662 Size: 354 83 98 14 129 186 196 28 Cuts at: 3662 3684 3708 3809 4551 4636 5707 1391 Size: 22 24 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 310 196 186 129 110 108 101 100 98 85 83 65 57 34 28 24 22 14 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 3150 3845 844 Size: 1781 525 695 3489 Fragments arranged by size: 3489 1781 695 525 BsrBI CCG'CTC Cuts at: 1394 1823 2158 2232 2462 2886 3112 4377 1394 Size: 429 335 74 230 424 226 1265 3507 Fragments arranged by size: 3507 1265 429 424 335 230 226 74 BsrDI GCAATG_nn' Cuts at: 65 1248 3406 4985 5159 65 Size: 1183 2158 1579 174 1396 Fragments arranged by size: 2158 1579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3221 3276 3733 5134 5321 1700 Size: 110 1411 55 457 1401 187 2869 Fragments arranged by size: 2869 1411 1401 457 187 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 2496 2664 2786 1925 Size: 571 168 122 5629 Fragments arranged by size: 5629 571 168 122 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 3088 3243 794 Size: 2294 155 4041 Fragments arranged by size: 4041 2294 155 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstXI CCAn_nnnn'nTGG Cuts at: 2584 2584 Size: 6490 BstYI r'GATC_y Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485 Size: 1848 367 385 17 1452 86 12 346 Cuts at: 6485 1972 Size: 1977 Fragments arranged by size: 1977 1848 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2682 3619 2682 Size: 937 5553 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2278 2398 2526 2541 2742 Size: 552 939 48 226 120 128 15 201 Cuts at: 2742 2769 2814 2910 3427 513 Size: 27 45 96 517 3576 Fragments arranged by size: 3576 939 552 517 226 201 128 120 96 48 45 27 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2638 2869 4006 4800 4820 2638 Size: 231 1137 794 20 4308 Fragments arranged by size: 4308 1137 794 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3388 3423 529 Size: 35 2824 35 3596 Fragments arranged by size: 3596 2824 35 35 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2481 2523 Size: 54 1425 50 275 18 384 267 42 Cuts at: 2523 2550 2691 2817 2907 2917 2946 2954 3223 Size: 27 141 126 90 10 29 8 269 Cuts at: 3223 3735 3786 3937 4826 6455 8 Size: 512 51 151 889 1629 43 Fragments arranged by size: 1629 1425 889 512 384 275 269 267 151 141 126 90 54 51 50 43 42 29 27 18 10 8 EagI C'GGCC_G Cuts at: 1537 1812 2214 2481 2523 2691 2917 2946 2954 Size: 275 402 267 42 168 226 29 8 Cuts at: 2954 3735 3937 1537 Size: 781 202 4090 Fragments arranged by size: 4090 781 402 275 267 226 202 168 42 29 8 EarI CTCTTCn'nnn_ Cuts at: 1353 2128 3568 4420 1353 Size: 775 1440 852 3423 Fragments arranged by size: 3423 1440 852 775 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2166 5077 5451 5597 387 Size: 1064 138 577 2911 374 146 1280 Fragments arranged by size: 2911 1280 1064 577 374 146 138 EcoICRI GAG'CTC Cuts at: 3682 3682 Size: 6490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2452 3167 4553 5150 5937 6205 Size: 393 427 393 715 1386 597 787 268 Cuts at: 6205 1239 Size: 1524 Fragments arranged by size: 1524 1386 787 715 597 427 393 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3146 3146 Size: 6490 EcoO109I rG'GnC_Cy Cuts at: 2161 2162 2306 2747 3128 3151 2161 Size: 1 144 441 381 23 5500 Fragments arranged by size: 5500 441 381 144 23 1 EcoRI G'AATT_C Cuts at: 3465 3799 4177 3465 Size: 334 378 5778 Fragments arranged by size: 5778 378 334 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2044 2175 2294 2406 2760 2829 3035 5637 Size: 366 131 119 112 354 69 206 2602 Cuts at: 5637 1678 Size: 2531 Fragments arranged by size: 2602 2531 366 354 206 131 119 112 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3140 3172 3649 3681 5131 Size: 32 135 32 1568 32 477 32 1450 Cuts at: 5131 5163 5205 5237 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1568 1450 477 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6490 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KasI G'GCGC_C Cuts at: 2171 2290 2402 2825 3031 2171 Size: 119 112 423 206 5630 Fragments arranged by size: 5630 423 206 119 112 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3575 5604 5788 1305 Size: 2270 2029 184 2007 Fragments arranged by size: 2270 2029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 10 Size: 54 1425 343 987 3681 Fragments arranged by size: 3681 1425 987 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3542 4448 4807 4966 518 Size: 648 1416 960 906 359 159 2042 Fragments arranged by size: 2042 1416 960 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2400 2528 2543 2715 2744 2771 2959 Size: 448 346 128 15 172 29 27 188 Cuts at: 2959 3202 3906 4583 5731 5976 1606 Size: 243 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 448 346 245 243 188 172 128 29 27 15 NaeI GCC'GGC Cuts at: 3278 3735 3278 Size: 457 6033 NarI GG'CG_CC Cuts at: 2172 2291 2403 2826 3032 2172 Size: 119 112 423 206 5630 Fragments arranged by size: 5630 423 206 119 112 NcoI C'CATG_G Cuts at: 513 1065 2004 2814 2910 3427 513 Size: 552 939 810 96 517 3576 Fragments arranged by size: 3576 939 810 552 517 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NgoMIV G'CCGG_C Cuts at: 3276 3733 3276 Size: 457 6033 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 2523 2917 2954 3937 2523 Size: 394 37 983 5076 Fragments arranged by size: 5076 983 394 37 NspI r_CATG'y Cuts at: 1132 1229 3848 5393 1132 Size: 97 2619 1545 2229 Fragments arranged by size: 2619 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 3366 1108 Size: 2258 4232 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3170 3202 4559 4591 6072 6104 3170 Size: 32 1357 32 1481 32 3556 Fragments arranged by size: 3556 1481 1357 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3128 3151 3128 Size: 23 6467 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PspOMI G'GGCC_C Cuts at: 2161 2305 2161 Size: 144 6346 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2954 2986 2954 Size: 32 6458 PstI C_TGCA'G Cuts at: 830 2622 3093 3842 830 Size: 1792 471 749 3478 Fragments arranged by size: 3478 1792 749 471 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 SacI G_AGCT'C Cuts at: 3684 3684 Size: 6490 SacII CC_GC'GG Cuts at: 2055 2401 2529 2544 2745 2772 2055 Size: 346 128 15 201 27 5773 Fragments arranged by size: 5773 346 201 128 27 15 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SanDI GG'GwC_CC Cuts at: 3128 3128 Size: 6490 SbfI CC_TGCA'GG Cuts at: 2622 3842 2622 Size: 1220 5270 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 2618 3089 3253 3612 3838 4973 5654 Size: 219 1573 471 164 359 226 1135 681 Cuts at: 5654 5845 826 Size: 191 1471 Fragments arranged by size: 1573 1471 1135 681 471 359 226 219 191 164 SfoI GGC'GCC Cuts at: 2173 2292 2404 2827 3033 2173 Size: 119 112 423 206 5630 Fragments arranged by size: 5630 423 206 119 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 2147 2311 3125 3817 1872 Size: 275 164 814 692 4545 Fragments arranged by size: 4545 814 692 275 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 3683 4594 5495 5757 820 Size: 197 926 1740 911 901 262 1553 Fragments arranged by size: 1740 1553 926 911 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 2687 1517 Size: 1170 5320 StyI C'CwwG_G Cuts at: 513 1065 2004 2814 2910 3427 3929 513 Size: 552 939 810 96 517 502 3074 Fragments arranged by size: 3074 939 810 552 517 502 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 4736 6448 Size: 274 80 33 51 1443 954 1805 1712 Cuts at: 6448 96 Size: 138 Fragments arranged by size: 1805 1712 1443 954 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3755 3787 6450 6482 3755 Size: 32 2663 32 3763 Fragments arranged by size: 3763 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2581 2812 2581 Size: 231 6259 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 2145 2309 3123 3815 1870 Size: 275 164 814 692 4545 Fragments arranged by size: 4545 814 692 275 164 XmnI GAAnn'nnTTC Cuts at: 2042 4619 2042 Size: 2577 3913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SapI SexAI SfiI SrfI SwaI