(Linear) (Six-base) MAPSORT of: KIBB9437.seq Check: 2643 from: 1 to: 1785 KIBB9437.orf With 182 enzymes: SgfI * April 28, 2014 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 620 1785 Size: 620 1165 AccI GT'mk_AC Cuts at: 0 560 1785 Size: 560 1225 AfeI AGC'GCT Cuts at: 0 37 1785 Size: 37 1748 AleI CACnn'nnGTG Cuts at: 0 577 1785 Size: 577 1208 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1165 1197 1785 Size: 1165 32 588 Fragments arranged by size: 1165 588 32 AlwNI CAG_nnn'CTG Cuts at: 0 176 1785 Size: 176 1609 ApaI G_GGCC'C Cuts at: 0 160 304 1785 Size: 160 144 1481 Fragments arranged by size: 1481 160 144 ApaLI G'TGCA_C Cuts at: 0 508 1785 Size: 508 1277 ApoI r'AATT_y Cuts at: 0 1460 1785 Size: 1460 325 AscI GG'CGCG_CC Cuts at: 0 491 1785 Size: 491 1294 AvaI C'yCGr_G Cuts at: 0 140 304 421 1118 1663 1785 Size: 140 164 117 697 545 122 Fragments arranged by size: 697 545 164 140 122 117 BanI G'GyrC_C Cuts at: 0 166 285 397 820 1026 1113 1213 1428 Size: 166 119 112 423 206 87 100 215 Cuts at: 1428 1624 1785 Size: 196 161 Fragments arranged by size: 423 215 206 196 166 161 119 112 100 87 BanII G_rGCy'C Cuts at: 0 95 160 270 304 412 923 1006 1104 Size: 95 65 110 34 108 511 83 98 Cuts at: 1104 1247 1679 1703 1785 Size: 143 432 24 82 Fragments arranged by size: 511 432 143 110 108 98 95 83 82 65 34 24 BbeI G_GCGC'C Cuts at: 0 170 289 401 824 1030 1785 Size: 170 119 112 423 206 755 Fragments arranged by size: 755 423 206 170 119 112 BbvCI CC'TCA_GC Cuts at: 0 188 1267 1785 Size: 188 1079 518 Fragments arranged by size: 1079 518 188 BglI GCCn_nnn'nGGC Cuts at: 0 164 308 526 541 1044 1200 1618 1729 Size: 164 144 218 15 503 156 418 111 Cuts at: 1729 1785 Size: 56 Fragments arranged by size: 503 418 218 164 156 144 111 56 15 BlpI GC'TnA_GC Cuts at: 0 406 759 1785 Size: 406 353 1026 Fragments arranged by size: 1026 406 353 Bme1580I G_kGCm'C Cuts at: 0 160 304 512 569 1118 1433 1629 1785 Size: 160 144 208 57 549 315 196 156 Fragments arranged by size: 549 315 208 196 160 156 144 57 BmgBI CAC'GTC Cuts at: 0 774 1742 1785 Size: 774 968 43 Fragments arranged by size: 968 774 43 BmrI ACTGGGnnnn_n' Cuts at: 0 918 1121 1785 Size: 918 203 664 Fragments arranged by size: 918 664 203 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1123 1785 Size: 1123 662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 447 1162 1785 Size: 54 393 715 623 Fragments arranged by size: 715 623 393 54 Bpu10I CC'TnA_GC Cuts at: 0 188 1267 1785 Size: 188 1079 518 Fragments arranged by size: 1079 518 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1663 1785 Size: 1663 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 1057 1785 Size: 1057 728 BsaAI yAC'GTr Cuts at: 0 564 1020 1785 Size: 564 456 765 Fragments arranged by size: 765 564 456 BsaHI Gr'CG_yC Cuts at: 0 59 167 184 286 398 821 1027 1036 Size: 59 108 17 102 112 423 206 9 Cuts at: 1036 1192 1712 1785 Size: 156 520 73 Fragments arranged by size: 520 423 206 156 112 108 102 73 59 17 9 BsaWI w'CCGG_w Cuts at: 0 1755 1785 Size: 1755 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 424 454 1135 1165 1195 1785 Size: 424 30 681 30 30 590 Fragments arranged by size: 681 590 424 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 120 448 1785 Size: 120 328 1337 Fragments arranged by size: 1337 328 120 BseYI C'CCAG_C Cuts at: 0 323 799 1785 Size: 323 476 986 Fragments arranged by size: 986 476 323 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1060 1151 1785 Size: 1060 91 634 Fragments arranged by size: 1060 634 91 BsiEI CG_ry'CG Cuts at: 0 212 368 479 521 689 915 944 952 Size: 212 156 111 42 168 226 29 8 Cuts at: 952 1733 1756 1785 Size: 781 23 29 Fragments arranged by size: 781 226 212 168 156 111 42 29 29 23 8 BsiHKAI G_wGCw'C Cuts at: 0 512 1657 1679 1785 Size: 512 1145 22 106 Fragments arranged by size: 1145 512 106 22 BsmBI CGTCTCn'nnnn_ Cuts at: 0 780 1785 Size: 780 1005 Bsp1286I G_dGCh'C Cuts at: 0 95 160 270 304 412 512 569 923 Size: 95 65 110 34 108 100 57 354 Cuts at: 923 1006 1104 1118 1247 1433 1629 1657 1679 Size: 83 98 14 129 186 196 28 22 Cuts at: 1679 1703 1785 Size: 24 82 Fragments arranged by size: 354 196 186 129 110 108 100 98 95 83 82 65 57 34 28 24 22 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 620 1145 1785 Size: 620 525 640 Fragments arranged by size: 640 620 525 BsrBI CCG'CTC Cuts at: 0 153 227 457 881 1107 1785 Size: 153 74 230 424 226 678 Fragments arranged by size: 678 424 230 226 153 74 BsrDI GCAATG_nn' Cuts at: 0 1401 1785 Size: 1401 384 BsrFI r'CCGG_y Cuts at: 0 1216 1271 1728 1785 Size: 1216 55 457 57 Fragments arranged by size: 1216 457 57 55 BssHII G'CGCG_C Cuts at: 0 491 659 781 1785 Size: 491 168 122 1004 Fragments arranged by size: 1004 491 168 122 BstAPI GCAn_nnn'nTGC Cuts at: 0 1083 1238 1785 Size: 1083 155 547 Fragments arranged by size: 1083 547 155 BstXI CCAn_nnnn'nTGG Cuts at: 0 579 1785 Size: 579 1206 Bsu36I CC'TnA_GG Cuts at: 0 677 1614 1785 Size: 677 937 171 Fragments arranged by size: 937 677 171 BtgI C'CryG_G Cuts at: 0 47 273 393 521 536 737 764 809 Size: 47 226 120 128 15 201 27 45 Cuts at: 809 905 1422 1785 Size: 96 517 363 Fragments arranged by size: 517 363 226 201 128 120 96 47 45 27 15 BtsI GCAGTG_nn' Cuts at: 0 633 864 1785 Size: 633 231 921 Fragments arranged by size: 921 633 231 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1383 1418 1785 Size: 1383 35 367 Fragments arranged by size: 1383 367 35 EaeI y'GGCC_r Cuts at: 0 209 476 518 545 686 812 902 912 Size: 209 267 42 27 141 126 90 10 Cuts at: 912 941 949 1218 1730 1785 Size: 29 8 269 512 55 Fragments arranged by size: 512 269 267 209 141 126 90 55 42 29 27 10 8 EagI C'GGCC_G Cuts at: 0 209 476 518 686 912 941 949 1730 Size: 209 267 42 168 226 29 8 781 Cuts at: 1730 1785 Size: 55 Fragments arranged by size: 781 267 226 209 168 55 42 29 8 EarI CTCTTCn'nnn_ Cuts at: 0 123 1563 1785 Size: 123 1440 222 Fragments arranged by size: 1440 222 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 161 1785 Size: 161 1624 EcoICRI GAG'CTC Cuts at: 0 1677 1785 Size: 1677 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 447 1162 1785 Size: 54 393 715 623 Fragments arranged by size: 715 623 393 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 1141 1785 Size: 1141 644 EcoO109I rG'GnC_Cy Cuts at: 0 156 157 301 742 1123 1146 1785 Size: 156 1 144 441 381 23 639 Fragments arranged by size: 639 441 381 156 144 23 1 EcoRI G'AATT_C Cuts at: 0 1460 1785 Size: 1460 325 HaeII r_GCGC'y Cuts at: 0 39 170 289 401 755 824 1030 1785 Size: 39 131 119 112 354 69 206 755 Fragments arranged by size: 755 354 206 131 119 112 69 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1135 1167 1644 1676 1785 Size: 1135 32 477 32 109 Fragments arranged by size: 1135 477 109 32 32 KasI G'GCGC_C Cuts at: 0 166 285 397 820 1026 1785 Size: 166 119 112 423 206 759 Fragments arranged by size: 759 423 206 166 119 112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1570 1785 Size: 1570 215 MscI TGG'CCA Cuts at: 0 814 1785 Size: 814 971 MslI CAynn'nnrTG Cuts at: 0 577 1537 1785 Size: 577 960 248 Fragments arranged by size: 960 577 248 MspA1I CmG'CkG Cuts at: 0 49 395 523 538 710 739 766 954 Size: 49 346 128 15 172 29 27 188 Cuts at: 954 1197 1785 Size: 243 588 Fragments arranged by size: 588 346 243 188 172 128 49 29 27 15 NaeI GCC'GGC Cuts at: 0 1273 1730 1785 Size: 1273 457 55 Fragments arranged by size: 1273 457 55 NarI GG'CG_CC Cuts at: 0 167 286 398 821 1027 1785 Size: 167 119 112 423 206 758 Fragments arranged by size: 758 423 206 167 119 112 NcoI C'CATG_G Cuts at: 0 809 905 1422 1785 Size: 809 96 517 363 Fragments arranged by size: 809 517 363 96 NgoMIV G'CCGG_C Cuts at: 0 1271 1728 1785 Size: 1271 457 57 Fragments arranged by size: 1271 457 57 NotI GC'GGCC_GC Cuts at: 0 518 912 949 1785 Size: 518 394 37 836 Fragments arranged by size: 836 518 394 37 PflMI CCAn_nnn'nTGG Cuts at: 0 1361 1785 Size: 1361 424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1165 1197 1785 Size: 1165 32 588 Fragments arranged by size: 1165 588 32 PpuMI rG'GwC_Cy Cuts at: 0 1123 1146 1785 Size: 1123 23 639 Fragments arranged by size: 1123 639 23 PspOMI G'GGCC_C Cuts at: 0 156 300 1785 Size: 156 144 1485 Fragments arranged by size: 1485 156 144 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 949 981 1785 Size: 949 32 804 Fragments arranged by size: 949 804 32 PstI C_TGCA'G Cuts at: 0 617 1088 1785 Size: 617 471 697 Fragments arranged by size: 697 617 471 SacI G_AGCT'C Cuts at: 0 1679 1785 Size: 1679 106 SacII CC_GC'GG Cuts at: 0 50 396 524 539 740 767 1785 Size: 50 346 128 15 201 27 1018 Fragments arranged by size: 1018 346 201 128 50 27 15 SanDI GG'GwC_CC Cuts at: 0 1123 1785 Size: 1123 662 SbfI CC_TGCA'GG Cuts at: 0 617 1785 Size: 617 1168 SfcI C'TryA_G Cuts at: 0 613 1084 1248 1607 1785 Size: 613 471 164 359 178 Fragments arranged by size: 613 471 359 178 164 SfoI GGC'GCC Cuts at: 0 168 287 399 822 1028 1785 Size: 168 119 112 423 206 757 Fragments arranged by size: 757 423 206 168 119 112 SmaI CCC'GGG Cuts at: 0 142 306 1120 1785 Size: 142 164 814 665 Fragments arranged by size: 814 665 164 142 SmlI C'TyrA_G Cuts at: 0 1678 1785 Size: 1678 107 StuI AGG'CCT Cuts at: 0 682 1785 Size: 682 1103 StyI C'CwwG_G Cuts at: 0 809 905 1422 1785 Size: 809 96 517 363 Fragments arranged by size: 809 517 363 96 TatI w'GTAC_w Cuts at: 0 926 1785 Size: 926 859 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1750 1782 1785 Size: 1750 32 3 Fragments arranged by size: 1750 32 3 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 576 807 1785 Size: 576 231 978 Fragments arranged by size: 978 576 231 XmaI C'CCGG_G Cuts at: 0 140 304 1118 1785 Size: 140 164 814 667 Fragments arranged by size: 814 667 164 140 XmnI GAAnn'nnTTC Cuts at: 0 37 1785 Size: 37 1748 Enzymes that do cut: AarI AccI AfeI AleI AloI AlwNI ApaI ApaLI ApoI AscI AvaI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PpiI PpuMI PspOMI PsrI PstI SacI SacII SanDI SbfI SfcI SfoI SmaI SmlI StuI StyI TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AlfI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspEI BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PvuI PvuII RsrII SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI ZraI