(Circular) (Six-base) MAPSORT of: pFN21AB9480.seq Check: 376 from: 1 to: 7201 pFN21AB9480 7201 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2980 3176 3932 2980 Size: 196 756 6249 Fragments arranged by size: 6249 756 196 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5011 278 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 2677 3489 3978 4544 1937 Size: 727 13 812 489 566 4594 Fragments arranged by size: 4594 812 727 566 489 13 Acc65I G'GTAC_C Cuts at: 4522 4522 Size: 7201 AclI AA'CG_TT Cuts at: 1563 5328 5701 1563 Size: 3765 373 3063 Fragments arranged by size: 3765 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2386 3053 3152 3986 5264 6648 6916 Size: 642 112 667 99 834 1278 1384 268 Cuts at: 6916 1632 Size: 1917 Fragments arranged by size: 1917 1384 1278 834 667 642 268 112 99 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7004 AflIII A'CryG_T Cuts at: 1203 3144 6100 7077 1203 Size: 1941 2956 977 1327 Fragments arranged by size: 2956 1941 1327 977 AgeI A'CCGG_T Cuts at: 2316 6032 2316 Size: 3716 3485 AhdI GACnn_n'nnGTC Cuts at: 4459 5930 4459 Size: 1471 5730 AleI CACnn'nnGTG Cuts at: 1166 4145 1166 Size: 2979 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 2540 1184 Size: 933 423 5845 Fragments arranged by size: 5845 933 423 AlwNI CAG_nnn'CTG Cuts at: 2110 3031 4253 4330 4944 6516 2110 Size: 921 1222 77 614 1572 2795 Fragments arranged by size: 2795 1572 1222 921 614 77 ApaLI G'TGCA_C Cuts at: 2450 5258 6414 2450 Size: 2808 1156 3237 Fragments arranged by size: 3237 2808 1156 ApoI r'AATT_y Cuts at: 1493 3647 4510 4736 4888 6997 1493 Size: 2154 863 226 152 2109 1697 Fragments arranged by size: 2154 2109 1697 863 226 152 AseI AT'TA_AT Cuts at: 160 1032 5755 160 Size: 872 4723 1606 Fragments arranged by size: 4723 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 AvaI C'yCGr_G Cuts at: 1870 1943 4036 4526 1870 Size: 73 2093 490 4545 Fragments arranged by size: 4545 2093 490 73 BamHI G'GATC_C Cuts at: 4531 4898 4531 Size: 367 6834 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 3701 4522 5977 618 Size: 325 217 627 246 1668 821 1455 1842 Fragments arranged by size: 1842 1668 1455 821 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 3951 4369 4379 4392 4520 1391 Size: 2560 418 10 13 128 4072 Fragments arranged by size: 4072 2560 418 128 13 10 BbeI G_GCGC'C Cuts at: 3705 3705 Size: 7201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3045 3886 928 Size: 971 1146 841 4243 Fragments arranged by size: 4243 1146 971 841 BbvCI CC'TCA_GC Cuts at: 3657 3730 3657 Size: 73 7128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2794 2828 5392 5426 1903 Size: 34 50 34 773 34 2564 34 3678 Fragments arranged by size: 3678 2564 773 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5093 6303 5093 Size: 1210 5991 BclI T'GATC_A Cuts at: 1548 2934 1548 Size: 1386 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3946 5812 136 Size: 107 122 71 631 605 2274 1866 1525 Fragments arranged by size: 2274 1866 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7196 7196 Size: 7201 BlpI GC'TnA_GC Cuts at: 4618 4618 Size: 7201 Bme1580I G_kGCm'C Cuts at: 1790 2036 2454 3942 4219 5262 6418 1790 Size: 246 418 1488 277 1043 1156 2573 Fragments arranged by size: 2573 1488 1156 1043 418 277 246 BmgBI CAC'GTC Cuts at: 1318 4131 4186 1318 Size: 2813 55 4333 Fragments arranged by size: 4333 2813 55 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 3271 3427 Size: 416 53 83 399 24 622 1232 156 Cuts at: 3427 4932 5890 442 Size: 1505 958 1753 Fragments arranged by size: 1753 1505 1232 958 622 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3289 3289 Size: 7201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 2573 2891 3041 3339 5861 1239 Size: 1087 247 318 150 298 2522 2579 Fragments arranged by size: 2579 2522 1087 318 298 247 150 Bpu10I CC'TnA_GC Cuts at: 3657 3730 4323 4378 3657 Size: 73 593 55 6480 Fragments arranged by size: 6480 593 73 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5326 6191 6489 5326 Size: 865 298 6038 Fragments arranged by size: 6038 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5864 882 Size: 617 4365 2219 Fragments arranged by size: 4365 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4992 493 Size: 711 3788 2702 Fragments arranged by size: 3788 2702 711 BsaBI GATnn'nnATC Cuts at: 7195 7195 Size: 7201 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2502 3702 4437 Size: 53 83 186 936 591 378 1200 735 Cuts at: 4437 5008 5390 6025 275 Size: 571 382 635 1451 Fragments arranged by size: 1451 1200 936 735 635 591 571 382 378 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 2316 5634 6032 6306 6453 6864 2190 Size: 126 3318 398 274 147 411 2527 Fragments arranged by size: 3318 2527 411 398 274 147 126 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6976 Fragments arranged by size: 6976 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3326 4430 1186 Size: 2140 1104 3957 Fragments arranged by size: 3957 2140 1104 BseYI C'CCAG_C Cuts at: 3598 6404 6900 3598 Size: 2806 496 3899 Fragments arranged by size: 3899 2806 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 2788 3148 3194 1163 Size: 573 873 179 360 46 5170 Fragments arranged by size: 5170 873 573 360 179 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3816 4651 5412 6440 1540 Size: 275 186 1815 835 761 1028 2301 Fragments arranged by size: 2301 1815 1028 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2454 3270 4239 4379 5262 5347 6418 2454 Size: 816 969 140 883 85 1071 3237 Fragments arranged by size: 3237 1071 969 883 816 140 85 BsmI GAATG_Cn' Cuts at: 4712 4805 4712 Size: 93 7108 BsmBI CGTCTCn'nnnn_ Cuts at: 2333 2333 Size: 7201 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2454 3270 3942 3951 4219 4239 Size: 399 246 418 816 672 9 268 20 Cuts at: 4239 4369 4379 4392 4520 5262 5347 6418 1391 Size: 130 10 13 128 742 85 1071 2174 Fragments arranged by size: 2174 1071 816 742 672 418 399 268 246 130 128 85 20 13 10 9 BspEI T'CCGG_A Cuts at: 2190 6864 2190 Size: 4674 2527 BspHI T'CATG_A Cuts at: 3906 5090 6820 3906 Size: 1184 1730 4287 Fragments arranged by size: 4287 1730 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2556 2622 2980 3176 3765 3932 4014 4556 Size: 1712 66 358 196 589 167 82 542 Cuts at: 4556 844 Size: 3489 Fragments arranged by size: 3489 1712 589 542 358 196 167 82 66 BsrBI CCG'CTC Cuts at: 1394 1823 2881 5088 1394 Size: 429 1058 2207 3507 Fragments arranged by size: 3507 2207 1058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 3723 4276 5696 5870 65 Size: 1183 1338 1137 553 1420 174 1396 Fragments arranged by size: 1420 1396 1338 1183 1137 553 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2316 4009 5845 6032 1700 Size: 110 506 1693 1836 187 2869 Fragments arranged by size: 2869 1836 1693 506 187 110 BsrGI T'GTAC_A Cuts at: 96 3783 3954 96 Size: 3687 171 3343 Fragments arranged by size: 3687 3343 171 BssHII G'CGCG_C Cuts at: 1925 3806 1925 Size: 1881 5320 BssSI C'ACGA_G Cuts at: 5261 6273 5261 Size: 1012 6189 BstAPI GCAn_nnn'nTGC Cuts at: 794 2110 794 Size: 1316 5885 BstBI TT'CG_AA Cuts at: 6005 6005 Size: 7201 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7201 BstXI CCAn_nnnn'nTGG Cuts at: 2137 3403 2137 Size: 1266 5935 BstYI r'GATC_y Cuts at: 1972 2421 4029 4531 4898 5283 5300 6752 6838 Size: 449 1608 502 367 385 17 1452 86 Cuts at: 6838 6850 7196 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1608 1452 502 449 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3172 3375 3750 513 Size: 552 2107 203 375 3964 Fragments arranged by size: 3964 2107 552 375 203 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2457 4303 487 Size: 683 56 1231 1846 3385 Fragments arranged by size: 3385 1846 1231 683 56 BtsI GCAGTG_nn' Cuts at: 2165 4284 4717 5511 5531 2165 Size: 2119 433 794 20 3835 Fragments arranged by size: 3835 2119 794 433 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7201 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2932 2967 529 Size: 35 2368 35 4763 Fragments arranged by size: 4763 2368 35 35 DraI TTT'AAA Cuts at: 4505 4505 Size: 7201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6208 809 Size: 507 4892 1802 Fragments arranged by size: 4892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2953 3813 Size: 54 1425 50 275 18 192 931 860 Cuts at: 3813 4011 4059 4648 5537 7166 8 Size: 198 48 589 889 1629 43 Fragments arranged by size: 1629 1425 931 889 860 589 275 198 192 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3813 4648 1537 Size: 275 2001 835 4090 Fragments arranged by size: 4090 2001 835 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 3332 3344 3626 4085 4097 5131 1353 Size: 1382 597 12 282 459 12 1034 3423 Fragments arranged by size: 3423 1382 1034 597 459 282 12 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5788 6162 6308 387 Size: 1064 138 4199 374 146 1280 Fragments arranged by size: 4199 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4377 4377 Size: 7201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2326 2386 2573 2891 3041 3053 Size: 393 642 52 60 187 318 150 12 Cuts at: 3053 3152 3339 3986 5264 5861 6648 6916 1239 Size: 99 187 647 1278 597 787 268 1524 Fragments arranged by size: 1524 1278 787 647 642 597 393 318 268 187 187 150 99 60 52 12 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 7201 EcoO109I rG'GnC_Cy Cuts at: 2107 2545 2107 Size: 438 6763 EcoRI G'AATT_C Cuts at: 4510 4888 4510 Size: 378 6823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2911 3667 2911 Size: 756 6445 FspI TGC'GCA Cuts at: 3764 4109 5707 3764 Size: 345 1598 5258 Fragments arranged by size: 5258 1598 345 FspAI rTGC'GCAy Cuts at: 4109 4109 Size: 7201 HaeII r_GCGC'y Cuts at: 1678 2082 3705 6348 1678 Size: 404 1623 2643 2531 Fragments arranged by size: 2643 2531 1623 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3273 3305 4379 4411 4445 Size: 32 135 32 1701 32 1074 32 34 Cuts at: 4445 4477 5842 5874 5916 5948 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1701 1365 1074 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2353 2533 4545 4791 669 Size: 988 281 415 180 2012 246 3079 Fragments arranged by size: 3079 2012 988 415 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 7201 HpaI GTT'AAC Cuts at: 4791 4791 Size: 7201 KasI G'GCGC_C Cuts at: 3701 3701 Size: 7201 KpnI G_GTAC'C Cuts at: 4526 4526 Size: 7201 MfeI C'AATT_G Cuts at: 4800 4800 Size: 7201 MluI A'CGCG_T Cuts at: 7077 7077 Size: 7201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3243 6315 6499 1305 Size: 1938 3072 184 2007 Fragments arranged by size: 3072 2007 1938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 2955 10 Size: 54 1425 343 192 931 4256 Fragments arranged by size: 4256 1425 931 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 3374 3575 3785 3935 3956 4133 4145 Size: 648 2208 201 210 150 21 177 12 Cuts at: 4145 5159 5518 5677 518 Size: 1014 359 159 2042 Fragments arranged by size: 2208 2042 1014 648 359 210 201 177 159 150 21 12 MspA1I CmG'CkG Cuts at: 1606 2470 3630 4617 5294 6442 6687 1606 Size: 864 1160 987 677 1148 245 2120 Fragments arranged by size: 2120 1160 1148 987 864 677 245 NaeI GCC'GGC Cuts at: 4011 4011 Size: 7201 NarI GG'CG_CC Cuts at: 3702 3702 Size: 7201 NcoI C'CATG_G Cuts at: 513 1065 3375 3750 513 Size: 552 2310 375 3964 Fragments arranged by size: 3964 2310 552 375 NdeI CA'TA_TG Cuts at: 387 387 Size: 7201 NgoMIV G'CCGG_C Cuts at: 4009 4009 Size: 7201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7201 NotI GC'GGCC_GC Cuts at: 4648 4648 Size: 7201 NspI r_CATG'y Cuts at: 1132 1229 2674 3961 4559 6104 1132 Size: 97 1445 1287 598 1545 2229 Fragments arranged by size: 2229 1545 1445 1287 598 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7201 PciI A'CATG_T Cuts at: 6100 6100 Size: 7201 PflMI CCAn_nnn'nTGG Cuts at: 1108 4057 1108 Size: 2949 4252 PfoI T'CCnGG_A Cuts at: 2424 2424 Size: 7201 PmeI GTTT'AAAC Cuts at: 4505 4505 Size: 7201 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5270 5302 6783 6815 3668 Size: 32 1570 32 1481 32 4054 Fragments arranged by size: 4054 1570 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2545 2545 Size: 7201 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7201 PsiI TTA'TAA Cuts at: 4771 4771 Size: 7201 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2549 2581 2549 Size: 32 7169 PstI C_TGCA'G Cuts at: 830 2106 3200 4335 4553 830 Size: 1276 1094 1135 218 3478 Fragments arranged by size: 3478 1276 1135 1094 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7201 SacI G_AGCT'C Cuts at: 4379 4379 Size: 7201 SalI G'TCGA_C Cuts at: 1936 4543 1936 Size: 2607 4594 SapI GCTCTTCn'nnn_ Cuts at: 4097 4097 Size: 7201 SbfI CC_TGCA'GG Cuts at: 2106 4553 2106 Size: 2447 4754 ScaI AGT'ACT Cuts at: 3008 5449 7161 3008 Size: 2441 1712 3048 Fragments arranged by size: 3048 2441 1712 SexAI A'CCwGG_T Cuts at: 3972 4050 4497 3972 Size: 78 447 6676 Fragments arranged by size: 6676 447 78 SfcI C'TryA_G Cuts at: 826 1045 2074 2102 2566 2665 3196 3913 4331 Size: 219 1029 28 464 99 531 717 418 Cuts at: 4331 4549 5684 6365 6556 826 Size: 218 1135 681 191 1471 Fragments arranged by size: 1471 1135 1029 717 681 531 464 418 219 218 191 99 28 SfoI GGC'GCC Cuts at: 3703 3703 Size: 7201 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7201 SmaI CCC'GGG Cuts at: 1872 4528 1872 Size: 2656 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5305 6206 6468 820 Size: 197 926 3362 901 262 1553 Fragments arranged by size: 3362 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4992 493 Size: 4499 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7201 SphI G_CATG'C Cuts at: 1132 2674 4559 1132 Size: 1542 1885 3774 Fragments arranged by size: 3774 1885 1542 SspI AAT'ATT Cuts at: 5 52 5125 5 Size: 47 5073 2081 Fragments arranged by size: 5073 2081 47 StuI AGG'CCT Cuts at: 1517 3774 3848 1517 Size: 2257 74 4870 Fragments arranged by size: 4870 2257 74 StyI C'CwwG_G Cuts at: 513 1065 2061 2223 3375 3750 4640 513 Size: 552 996 162 1152 375 890 3074 Fragments arranged by size: 3074 1152 996 890 552 375 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2280 3446 5228 5245 5398 5583 6028 Size: 1255 700 1166 1782 17 153 185 445 Cuts at: 6028 325 Size: 1498 Fragments arranged by size: 1782 1498 1255 1166 700 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2892 3006 3783 Size: 274 80 33 51 1443 915 114 777 Cuts at: 3783 3954 5447 7159 96 Size: 171 1493 1712 138 Fragments arranged by size: 1712 1493 1443 915 777 274 171 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2444 2476 7161 7193 2444 Size: 32 4685 32 2452 Fragments arranged by size: 4685 2452 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3229 1316 Size: 1913 5288 XbaI T'CTAG_A Cuts at: 4537 4537 Size: 7201 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2369 4190 2369 Size: 1821 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7201 XmaI C'CCGG_G Cuts at: 1870 4526 1870 Size: 2656 4545 XmnI GAAnn'nnTTC Cuts at: 3137 5330 3137 Size: 2193 5008 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5009 276 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SfiI SrfI SwaI