Restriction map for
pFN21AB9558
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BalI BbrPI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsiWI BsmBI BsmI BspDI
BspLU11I BspMI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 315 TCCGGA T^CCGGA
AgeI 1016 ACCGGT A^CCGGT
AhdI 380 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 946 CCTCAGG CC^T[ACGT]AGG
AspEI 380 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1277 GAGCAC G[AT]GC[AT]^C
AviII 221 TGCGCA TGC^GCA
BbeI 1035 GGCGCC GGCGC^C
BbvCI 174 CCTCAGC CCTCAGC(-5/-2)
BcgI 1106 CGAAGGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 1968 GAGACC GGTCTC(1/5)
BseAI 315 TCCGGA T^CCGGA
BsiHKAI 1277 GAGCAC G[AT]GC[AT]^C
Bsp1407I 671 TGTACA T^GTACA
BspEI 315 TCCGGA T^CCGGA
BsrBI 321 GAGCGG CCGCTC(-3/-3)
BsrDI 667 GCAATG GCAATG(2/0)
BsrGI 671 TGTACA T^GTACA
BssSI 449 CACGAG CACGAG(-5/-1)
BstEII 336 GGTGACC G^GT[ACGT]ACC
BstPI 336 GGTGACC G^GT[ACGT]ACC
Bsu36I 946 CCTCAGG CC^T[ACGT]AGG
Eam1105I 380 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 889 GAAGAG CTCTTC(1/4)
Eco81I 946 CCTCAGG CC^T[ACGT]AGG
EcoO65I 336 GGTGACC G^GT[ACGT]ACC
EcoRI 1529 GAATTC G^AATTC
FauI 402 GCGGG CCCGC(4/6)
FspI 221 TGCGCA TGC^GCA
HaeII 1035 GGCGCC [GA]GCGC^[CT]
HgaI 4 GCGTC GACGC(5/10)
KasI 1035 GGCGCC G^GCGCC
Ksp632I 889 GAAGAG CTCTTC(1/4)
MroI 315 TCCGGA T^CCGGA
MseI 1852 TTAA T^TAA
NaeI 179 GCCGGC GCC^GGC
NarI 1035 GGCGCC GG^CGCC
NgoMIV 179 GCCGGC G^CCGGC
PflMI 1045 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1016 ACCGGT A^CCGGT
SfoI 1035 GGCGCC GGC^GCC
SmlI 1080 CTCAAG C^T[CT][GA]AG
SphI 1760 GCATGC GCATG^C
SspBI 671 TGTACA T^GTACA
Tru9I 1852 TTAA T^TAA
Van91I 1045 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 147 CCAAGAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
1035 GGCGCC
AfaI 672 GTAC GT^AC
1814 GTAC
BamHI 702 GGATCC G^GATCC
831 GGATCC
BbsI 616 GAAGAC GAAGAC(2/6)
1004 GAAGAC
BfaI 523 CTAG C^TAG
707 CTAG
BglI 866 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1699 GCCCATAAGGC
BglII 236 AGATCT A^GATCT
1490 AGATCT
BpuAI 616 GAAGAC GAAGAC(2/6)
1004 GAAGAC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
1035 GGCGCC
BsaWI 315 TCCGGA [AT]^CCGG[AT]
1016 ACCGGT
BsiEI 1018 CGGTCG CG[GA][CT]^CG
1102 CGACCG
BsrFI 179 GCCGGC [GA]^CCGG[CT]
1016 ACCGGT
Cfr10I 179 GCCGGC [GA]^CCGG[CT]
1016 ACCGGT
Hin1I 3 GGCGTC G[GA]^CG[CT]C
1035 GGCGCC
HindIII 1746 AAGCTT A^AGCTT
1998 AAGCTT
MaeI 523 CTAG C^TAG
707 CTAG
MaeII 662 ACGT A^CGT
1257 ACGT
MslI 801 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1366 CACACGGGTG
MspA1I 155 CAGCTG C[AC]G^C[GT]G
1202 CAGCTG
NspI 1197 ACATGC [GA]CATG^[CT]
1760 GCATGC
PpuMI 542 GGGACCC [GA]G^G[AT]CC[CT]
791 AGGTCCC
PstI 511 CTGCAG CTGCA^G
1114 CTGCAG
PvuII 155 CAGCTG CAG^CTG
1202 CAGCTG
RsaI 672 GTAC GT^AC
1814 GTAC
SfcI 511 CTGCAG C^T[GA][CT]AG
1114 CTGCAG
SmaI 12 CCCGGG CCC^GGG
164 CCCGGG
StuI 113 AGGCCT AGG^CCT
1064 AGGCCT
TfiI 1868 GAATC G^A[AT]TC
1938 GATTC
XmaCI 12 CCCGGG C^CCGGG
164 CCCGGG
XmaI 12 CCCGGG C^CCGGG
164 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApaI ApoI Asp700I AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI RcaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
Tsp509I TspRI XhoII XmnI