(Circular) (Six-base) MAPSORT of: pFN21AB9558.seq Check: 498 from: 1 to: 6754 pFN21AB9558 6754 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 2628 3316 4817 6201 6469 1632 Size: 487 509 688 1501 1384 268 1917 Fragments arranged by size: 1917 1501 1384 688 509 487 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 3021 5585 3021 Size: 2564 4190 AhdI GACnn_n'nnGTC Cuts at: 2390 5483 2390 Size: 3093 3661 AleI CACnn'nnGTG Cuts at: 1166 2810 3375 4050 1166 Size: 1644 565 675 3870 Fragments arranged by size: 3870 1644 675 565 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6754 AlwNI CAG_nnn'CTG Cuts at: 2086 2272 2314 2524 2959 4497 6069 2086 Size: 186 42 210 435 1538 1572 2771 Fragments arranged by size: 2771 1572 1538 435 210 186 42 ApaI G_GGCC'C Cuts at: 2018 2643 2725 2018 Size: 625 82 6047 Fragments arranged by size: 6047 625 82 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 2900 3160 3534 4063 4289 4441 6550 1493 Size: 1407 260 374 529 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 529 374 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2114 2169 2976 3566 4079 1870 Size: 73 74 97 55 807 590 513 4545 Fragments arranged by size: 4545 807 590 513 97 74 73 55 BamHI G'GATC_C Cuts at: 2707 2836 4084 4451 2707 Size: 129 1248 367 5010 Fragments arranged by size: 5010 1248 367 129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2804 2986 3040 3189 3441 Size: 325 217 627 1017 182 54 149 252 Cuts at: 3441 3525 3864 4075 5530 618 Size: 84 339 211 1455 1842 Fragments arranged by size: 1842 1455 1017 627 339 325 252 217 211 182 149 84 54 BanII G_rGCy'C Cuts at: 1391 2018 2024 2043 2114 2643 2725 2873 3706 Size: 627 6 19 71 529 82 148 833 Cuts at: 3706 4073 1391 Size: 367 4072 Fragments arranged by size: 4072 833 627 529 367 148 82 71 19 6 BbeI G_GCGC'C Cuts at: 3044 3044 Size: 6754 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2628 3016 928 Size: 971 729 388 4666 Fragments arranged by size: 4666 971 729 388 BbvCI CC'TCA_GC Cuts at: 2180 2180 Size: 6754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3100 3134 4945 4979 1903 Size: 34 1163 34 1811 34 3678 Fragments arranged by size: 3678 1811 1163 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2877 3710 5365 Size: 107 122 71 631 605 1205 833 1655 Cuts at: 5365 136 Size: 1525 Fragments arranged by size: 1655 1525 1205 833 631 605 122 107 71 BglII A'GATC_T Cuts at: 2241 3495 6749 2241 Size: 1254 3254 2246 Fragments arranged by size: 3254 2246 1254 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 2018 2643 2725 2991 3482 4815 5971 1790 Size: 228 625 82 266 491 1333 1156 2573 Fragments arranged by size: 2573 1333 1156 625 491 266 228 82 BmgBI CAC'GTC Cuts at: 1318 2668 1318 Size: 1350 5404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2638 2646 2781 Size: 416 53 83 399 24 1221 8 135 Cuts at: 2781 2835 3110 3468 4485 5443 442 Size: 54 275 358 1017 958 1753 Fragments arranged by size: 1753 1221 1017 958 416 399 358 275 135 83 54 53 24 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2095 2498 2713 2792 2871 3013 3564 Size: 839 17 403 215 79 79 142 551 Cuts at: 3564 5414 1239 Size: 1850 2579 Fragments arranged by size: 2579 1850 839 551 403 215 142 79 79 17 Bpu10I CC'TnA_GC Cuts at: 2180 2867 3000 2180 Size: 687 133 5934 Fragments arranged by size: 5934 687 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3070 4879 5744 6042 3070 Size: 1809 865 298 3782 Fragments arranged by size: 3782 1809 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3967 5417 882 Size: 617 2468 1450 2219 Fragments arranged by size: 2468 2219 1450 617 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3041 4561 4943 Size: 53 83 186 936 476 1032 1520 382 Cuts at: 4943 5578 275 Size: 635 1451 Fragments arranged by size: 1520 1451 1032 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2320 3021 5187 5585 5859 6006 6417 2320 Size: 701 2166 398 274 147 411 2657 Fragments arranged by size: 2657 2166 701 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4006 4036 1178 Size: 30 165 30 2603 30 3896 Fragments arranged by size: 3896 2603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2444 2705 3311 1186 Size: 889 369 261 606 4629 Fragments arranged by size: 4629 889 606 369 261 BseYI C'CCAG_C Cuts at: 2534 2881 3004 5957 6453 2534 Size: 347 123 2953 496 2835 Fragments arranged by size: 2953 2835 496 347 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2762 3220 1163 Size: 573 591 435 458 4697 Fragments arranged by size: 4697 591 573 458 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3026 3110 4204 4965 5993 1540 Size: 275 186 1025 84 1094 761 1028 2301 Fragments arranged by size: 2301 1094 1028 1025 761 275 186 84 BsiHKAI G_wGCw'C Cuts at: 3286 4815 4900 5971 3286 Size: 1529 85 1071 4069 Fragments arranged by size: 4069 1529 1071 85 BsmI GAATG_Cn' Cuts at: 4265 4358 4265 Size: 93 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2024 2043 2114 2643 2725 2873 Size: 399 228 6 19 71 529 82 148 Cuts at: 2873 2991 3286 3482 3706 4073 4815 4900 5971 Size: 118 295 196 224 367 742 85 1071 Cuts at: 5971 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 529 399 367 295 228 224 196 148 118 85 82 71 19 6 BspEI T'CCGG_A Cuts at: 2320 6417 2320 Size: 4097 2657 BspHI T'CATG_A Cuts at: 3016 3622 3904 4643 6373 3016 Size: 606 282 739 1730 3397 Fragments arranged by size: 3397 1730 739 606 282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4109 844 Size: 3265 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2328 4641 1394 Size: 429 505 2313 3507 Fragments arranged by size: 3507 2313 505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2679 5249 5423 65 Size: 1183 1431 2570 174 1396 Fragments arranged by size: 2570 1431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 3021 5398 5585 1700 Size: 110 374 837 2377 187 2869 Fragments arranged by size: 2869 2377 837 374 187 110 BsrGI T'GTAC_A Cuts at: 96 2676 96 Size: 2580 4174 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 2454 4814 5826 2454 Size: 2360 1012 3382 Fragments arranged by size: 3382 2360 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 2524 2579 3402 3766 794 Size: 1520 210 55 823 364 3782 Fragments arranged by size: 3782 1520 823 364 210 55 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 2341 1184 Size: 1157 5597 BstYI r'GATC_y Cuts at: 1972 2241 2292 2707 2836 3495 3848 4084 4451 Size: 269 51 415 129 659 353 236 367 Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 659 415 385 367 353 346 269 236 129 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2952 2952 Size: 6754 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 2401 3697 513 Size: 552 939 199 198 1296 3570 Fragments arranged by size: 3570 1296 939 552 199 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 4270 5064 5084 4270 Size: 794 20 5940 Fragments arranged by size: 5940 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719 Size: 54 1425 50 275 18 2371 889 1629 Cuts at: 6719 8 Size: 43 Fragments arranged by size: 2371 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 4684 1353 Size: 1536 1795 3423 Fragments arranged by size: 3423 1795 1536 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2095 2119 2498 2628 2713 2792 Size: 393 446 17 24 379 130 85 79 Cuts at: 2792 2871 3013 3316 3564 4817 5414 6201 6469 Size: 79 142 303 248 1253 597 787 268 Cuts at: 6469 1239 Size: 1524 Fragments arranged by size: 1524 1253 787 597 446 393 379 303 268 248 142 130 85 79 79 24 17 EcoNI CCTnn'n_nnAGG Cuts at: 2100 2285 2351 2607 3293 2100 Size: 185 66 256 686 5561 Fragments arranged by size: 5561 686 256 185 66 EcoO109I rG'GnC_Cy Cuts at: 2531 2548 2640 2721 2722 2797 2863 2531 Size: 17 92 81 1 75 66 6422 Fragments arranged by size: 6422 92 81 75 66 17 1 EcoRI G'AATT_C Cuts at: 3534 4063 4441 3534 Size: 529 378 5847 Fragments arranged by size: 5847 529 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3742 3742 Size: 6754 FspI TGC'GCA Cuts at: 2228 5260 2228 Size: 3032 3722 FspAI rTGC'GCAy Cuts at: 2228 2228 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 3044 5901 1678 Size: 1366 2857 2531 Fragments arranged by size: 2857 2531 1366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 3487 3519 3908 Size: 32 135 32 804 32 1079 32 389 Cuts at: 3908 3940 4006 4038 5395 5427 5469 5501 1373 Size: 32 66 32 1357 32 42 32 2626 Fragments arranged by size: 2626 1357 1079 804 389 135 66 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4098 4344 669 Size: 988 281 2160 246 3079 Fragments arranged by size: 3079 2160 988 281 246 HindIII A'AGCT_T Cuts at: 748 3751 4003 748 Size: 3003 252 3499 Fragments arranged by size: 3499 3003 252 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KasI G'GCGC_C Cuts at: 3040 3040 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3453 3537 5868 6052 1305 Size: 2148 84 2331 184 2007 Fragments arranged by size: 2331 2148 2007 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4932 Fragments arranged by size: 4932 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3375 4050 4712 5071 5230 518 Size: 648 1644 565 675 662 359 159 2042 Fragments arranged by size: 2042 1644 675 662 648 565 359 159 MspA1I CmG'CkG Cuts at: 1606 2162 3209 4170 4847 5995 6240 1606 Size: 556 1047 961 677 1148 245 2120 Fragments arranged by size: 2120 1148 1047 961 677 556 245 NaeI GCC'GGC Cuts at: 2186 2186 Size: 6754 NarI GG'CG_CC Cuts at: 3041 3041 Size: 6754 NcoI C'CATG_G Cuts at: 513 1065 2004 2203 2401 3697 513 Size: 552 939 199 198 1296 3570 Fragments arranged by size: 3570 1296 939 552 199 198 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NgoMIV G'CCGG_C Cuts at: 2184 2184 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 3206 3769 4112 5657 1132 Size: 97 1977 563 343 1545 2229 Fragments arranged by size: 2229 1977 1545 563 343 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6754 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 3056 1108 Size: 1948 4806 PfoI T'CCnGG_A Cuts at: 2887 2887 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2797 2548 Size: 249 6505 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 3558 4324 3558 Size: 766 5988 PspOMI G'GGCC_C Cuts at: 2014 2639 2721 2014 Size: 625 82 6047 Fragments arranged by size: 6047 625 82 PstI C_TGCA'G Cuts at: 830 2520 3123 4106 830 Size: 1690 603 983 3478 Fragments arranged by size: 3478 1690 983 603 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2162 3209 2162 Size: 1047 5707 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SanDI GG'GwC_CC Cuts at: 2548 2548 Size: 6754 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2516 3119 4102 5237 5918 6109 826 Size: 219 1471 603 983 1135 681 191 1471 Fragments arranged by size: 1471 1471 1135 983 681 603 219 191 SfoI GGC'GCC Cuts at: 3042 3042 Size: 6754 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 2019 2171 4081 1872 Size: 147 152 1910 4545 Fragments arranged by size: 4545 1910 152 147 SmlI C'TyrA_G Cuts at: 820 1017 1943 3085 4858 5759 6021 820 Size: 197 926 1142 1773 901 262 1553 Fragments arranged by size: 1773 1553 1142 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 3769 4112 1132 Size: 2637 343 3774 Fragments arranged by size: 3774 2637 343 SrfI GCCC'GGGC Cuts at: 2019 2019 Size: 6754 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 2120 3071 1517 Size: 603 951 5200 Fragments arranged by size: 5200 951 603 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 2401 3072 3697 4193 513 Size: 552 939 199 198 671 625 496 3074 Fragments arranged by size: 3074 939 671 625 552 496 199 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2122 2196 2822 3124 3215 3359 3383 Size: 1255 542 74 626 302 91 144 24 Cuts at: 3383 4781 4798 4951 5136 5581 325 Size: 1398 17 153 185 445 1498 Fragments arranged by size: 1498 1398 1255 626 542 445 302 185 153 144 91 74 24 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2676 5000 6712 Size: 274 80 33 51 1443 699 2324 1712 Cuts at: 6712 96 Size: 138 Fragments arranged by size: 2324 1712 1443 699 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 6714 6746 2403 Size: 32 4279 32 2411 Fragments arranged by size: 4279 2411 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2159 2159 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 2017 2169 4079 1870 Size: 147 152 1910 4545 Fragments arranged by size: 4545 1910 152 147 XmnI GAAnn'nnTTC Cuts at: 2919 3329 3581 3752 4883 2919 Size: 410 252 171 1131 4790 Fragments arranged by size: 4790 1131 410 252 171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SwaI