Restriction map for
pFN21AB9558
(Linear) (Six-base) MAPSORT of: KIBB9558.seq Check: 4106 from: 1 to: 2049
KIBB9558.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 623 1311 2049
Size: 114 509 688 738
Fragments arranged by size:
738 688 509 114
AgeI A'CCGG_T
Cuts at: 0 1016 2049
Size: 1016 1033
AhdI GACnn_n'nnGTC
Cuts at: 0 385 2049
Size: 385 1664
AleI CACnn'nnGTG
Cuts at: 0 805 1370 2049
Size: 805 565 679
Fragments arranged by size:
805 679 565
AlwNI CAG_nnn'CTG
Cuts at: 0 81 267 309 519 954 2049
Size: 81 186 42 210 435 1095
Fragments arranged by size:
1095 435 210 186 81 42
ApaI G_GGCC'C
Cuts at: 0 13 638 720 2049
Size: 13 625 82 1329
Fragments arranged by size:
1329 625 82 13
ApoI r'AATT_y
Cuts at: 0 895 1155 1529 2049
Size: 895 260 374 520
Fragments arranged by size:
895 520 374 260
AvaI C'yCGr_G
Cuts at: 0 12 109 164 971 1561 2049
Size: 12 97 55 807 590 488
Fragments arranged by size:
807 590 488 97 55 12
BamHI G'GATC_C
Cuts at: 0 702 831 2049
Size: 702 129 1218
Fragments arranged by size:
1218 702 129
BanI G'GyrC_C
Cuts at: 0 799 981 1035 1184 1436 1520 1859 2049
Size: 799 182 54 149 252 84 339 190
Fragments arranged by size:
799 339 252 190 182 149 84 54
BanII G_rGCy'C
Cuts at: 0 13 19 38 109 638 720 868 1701
Size: 13 6 19 71 529 82 148 833
Cuts at: 1701 2049
Size: 348
Fragments arranged by size:
833 529 348 148 82 71 19 13
6
BbeI G_GCGC'C
Cuts at: 0 1039 2049
Size: 1039 1010
BbsI GAAGACnn'nnnn_
Cuts at: 0 623 1011 2049
Size: 623 388 1038
Fragments arranged by size:
1038 623 388
BbvCI CC'TCA_GC
Cuts at: 0 175 2049
Size: 175 1874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1095 1129 2049
Size: 1095 34 920
Fragments arranged by size:
1095 920 34
BglI GCCn_nnn'nGGC
Cuts at: 0 872 1705 2049
Size: 872 833 344
Fragments arranged by size:
872 833 344
BglII A'GATC_T
Cuts at: 0 236 1490 2049
Size: 236 1254 559
Fragments arranged by size:
1254 559 236
Bme1580I G_kGCm'C
Cuts at: 0 13 638 720 986 1477 2049
Size: 13 625 82 266 491 572
Fragments arranged by size:
625 572 491 266 82 13
BmgBI CAC'GTC
Cuts at: 0 663 2049
Size: 663 1386
BmrI ACTGGGnnnn_n'
Cuts at: 0 633 641 776 830 1105 1463 2049
Size: 633 8 135 54 275 358 586
Fragments arranged by size:
633 586 358 275 135 54 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 90 493 708 787 866 1008 1559
Size: 73 17 403 215 79 79 142 551
Cuts at: 1559 2049
Size: 490
Fragments arranged by size:
551 490 403 215 142 79 79 73
17
Bpu10I CC'TnA_GC
Cuts at: 0 175 862 995 2049
Size: 175 687 133 1054
Fragments arranged by size:
1054 687 175 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 2049
Size: 1065 984
BsaI GGTCTCn'nnnn_
Cuts at: 0 1962 2049
Size: 1962 87
BsaHI Gr'CG_yC
Cuts at: 0 4 1036 2049
Size: 4 1032 1013
Fragments arranged by size:
1032 1013 4
BsaWI w'CCGG_w
Cuts at: 0 315 1016 2049
Size: 315 701 1033
Fragments arranged by size:
1033 701 315
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2001 2031 2049
Size: 2001 30 18
Fragments arranged by size:
2001 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 439 700 1306 2049
Size: 70 369 261 606 743
Fragments arranged by size:
743 606 369 261 70
BseYI C'CCAG_C
Cuts at: 0 529 876 999 2049
Size: 529 347 123 1050
Fragments arranged by size:
1050 529 347 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 757 1215 2049
Size: 322 435 458 834
Fragments arranged by size:
834 458 435 322
BsiEI CG_ry'CG
Cuts at: 0 1021 1105 2049
Size: 1021 84 944
Fragments arranged by size:
1021 944 84
BsiHKAI G_wGCw'C
Cuts at: 0 1281 2049
Size: 1281 768
Bsp1286I G_dGCh'C
Cuts at: 0 13 19 38 109 638 720 868 986
Size: 13 6 19 71 529 82 148 118
Cuts at: 986 1281 1477 1701 2049
Size: 295 196 224 348
Fragments arranged by size:
529 348 295 224 196 148 118 82
71 19 13 6
BspEI T'CCGG_A
Cuts at: 0 315 2049
Size: 315 1734
BspHI T'CATG_A
Cuts at: 0 1011 1617 1899 2049
Size: 1011 606 282 150
Fragments arranged by size:
1011 606 282 150
BsrBI CCG'CTC
Cuts at: 0 323 2049
Size: 323 1726
BsrDI GCAATG_nn'
Cuts at: 0 674 2049
Size: 674 1375
BsrFI r'CCGG_y
Cuts at: 0 179 1016 2049
Size: 179 837 1033
Fragments arranged by size:
1033 837 179
BsrGI T'GTAC_A
Cuts at: 0 671 2049
Size: 671 1378
BssSI C'ACGA_G
Cuts at: 0 449 2049
Size: 449 1600
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 519 574 1397 1761 2049
Size: 309 210 55 823 364 288
Fragments arranged by size:
823 364 309 288 210 55
BstEII G'GTnAC_C
Cuts at: 0 336 2049
Size: 336 1713
BstYI r'GATC_y
Cuts at: 0 236 287 702 831 1490 1843 2049
Size: 236 51 415 129 659 353 206
Fragments arranged by size:
659 415 353 236 206 129 51
Bsu36I CC'TnA_GG
Cuts at: 0 947 2049
Size: 947 1102
BtgI C'CryG_G
Cuts at: 0 198 396 1692 2049
Size: 198 198 1296 357
Fragments arranged by size:
1296 357 198 198
EarI CTCTTCn'nnn_
Cuts at: 0 884 2049
Size: 884 1165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 90 114 493 623 708 787 866
Size: 73 17 24 379 130 85 79 79
Cuts at: 866 1008 1311 1559 2049
Size: 142 303 248 490
Fragments arranged by size:
490 379 303 248 142 130 85 79
79 73 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 95 280 346 602 1288 2049
Size: 95 185 66 256 686 761
Fragments arranged by size:
761 686 256 185 95 66
EcoO109I rG'GnC_Cy
Cuts at: 0 526 543 635 716 717 792 858 2049
Size: 526 17 92 81 1 75 66 1191
Fragments arranged by size:
1191 526 92 81 75 66 17 1
EcoRI G'AATT_C
Cuts at: 0 1529 2049
Size: 1529 520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1737 2049
Size: 1737 312
FspI TGC'GCA
Cuts at: 0 223 2049
Size: 223 1826
FspAI rTGC'GCAy
Cuts at: 0 223 2049
Size: 223 1826
HaeII r_GCGC'y
Cuts at: 0 1039 2049
Size: 1039 1010
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1482 1514 1903 1935 2001 2033
Size: 371 32 1079 32 389 32 66 32
Cuts at: 2033 2049
Size: 16
Fragments arranged by size:
1079 389 371 66 32 32 32 32
16
HindIII A'AGCT_T
Cuts at: 0 1746 1998 2049
Size: 1746 252 51
Fragments arranged by size:
1746 252 51
KasI G'GCGC_C
Cuts at: 0 1035 2049
Size: 1035 1014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1448 1532 2049
Size: 1448 84 517
Fragments arranged by size:
1448 517 84
MslI CAynn'nnrTG
Cuts at: 0 805 1370 2049
Size: 805 565 679
Fragments arranged by size:
805 679 565
MspA1I CmG'CkG
Cuts at: 0 157 1204 2049
Size: 157 1047 845
Fragments arranged by size:
1047 845 157
NaeI GCC'GGC
Cuts at: 0 181 2049
Size: 181 1868
NarI GG'CG_CC
Cuts at: 0 1036 2049
Size: 1036 1013
NcoI C'CATG_G
Cuts at: 0 198 396 1692 2049
Size: 198 198 1296 357
Fragments arranged by size:
1296 357 198 198
NgoMIV G'CCGG_C
Cuts at: 0 179 2049
Size: 179 1870
NspI r_CATG'y
Cuts at: 0 1201 1764 2049
Size: 1201 563 285
Fragments arranged by size:
1201 563 285
PflMI CCAn_nnn'nTGG
Cuts at: 0 1051 2049
Size: 1051 998
PfoI T'CCnGG_A
Cuts at: 0 882 2049
Size: 882 1167
PpuMI rG'GwC_Cy
Cuts at: 0 543 792 2049
Size: 543 249 1257
Fragments arranged by size:
1257 543 249
PsiI TTA'TAA
Cuts at: 0 1553 2049
Size: 1553 496
PspOMI G'GGCC_C
Cuts at: 0 9 634 716 2049
Size: 9 625 82 1333
Fragments arranged by size:
1333 625 82 9
PstI C_TGCA'G
Cuts at: 0 515 1118 2049
Size: 515 603 931
Fragments arranged by size:
931 603 515
PvuII CAG'CTG
Cuts at: 0 157 1204 2049
Size: 157 1047 845
Fragments arranged by size:
1047 845 157
SanDI GG'GwC_CC
Cuts at: 0 543 2049
Size: 543 1506
SfcI C'TryA_G
Cuts at: 0 511 1114 2049
Size: 511 603 935
Fragments arranged by size:
935 603 511
SfoI GGC'GCC
Cuts at: 0 1037 2049
Size: 1037 1012
SmaI CCC'GGG
Cuts at: 0 14 166 2049
Size: 14 152 1883
Fragments arranged by size:
1883 152 14
SmlI C'TyrA_G
Cuts at: 0 1080 2049
Size: 1080 969
SphI G_CATG'C
Cuts at: 0 1764 2049
Size: 1764 285
SrfI GCCC'GGGC
Cuts at: 0 14 2049
Size: 14 2035
StuI AGG'CCT
Cuts at: 0 115 1066 2049
Size: 115 951 983
Fragments arranged by size:
983 951 115
StyI C'CwwG_G
Cuts at: 0 198 396 1067 1692 2049
Size: 198 198 671 625 357
Fragments arranged by size:
671 625 357 198 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 191 817 1119 1210 1354 1378 2049
Size: 117 74 626 302 91 144 24 671
Fragments arranged by size:
671 626 302 144 117 91 74 24
TatI w'GTAC_w
Cuts at: 0 671 2049
Size: 671 1378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 398 430 2049
Size: 398 32 1619
Fragments arranged by size:
1619 398 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 2049
Size: 154 1895
XmaI C'CCGG_G
Cuts at: 0 12 164 2049
Size: 12 152 1885
Fragments arranged by size:
1885 152 12
XmnI GAAnn'nnTTC
Cuts at: 0 914 1324 1576 1747 2049
Size: 914 410 252 171 302
Fragments arranged by size:
914 410 302 252 171
Enzymes that do cut:
AcuI AgeI AhdI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstYI Bsu36I BtgI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HindIII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII
SanDI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BlpI BmtI BplI BsaAI BsaBI
BsiWI BsmI BsmBI BspMI BssHII BstBI BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRV FseI HincII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI ZraI