Restriction map for
pFN21AB8733
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1082 GTCGAC GT^[AC][GT]AC
AflII 775 CTTAAG C^TTAAG
AlwNI 487 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 407 GGGCCC GGGCC^C
Asp700I 592 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 541 GAGCTC G[AT]GC[AT]^C
BbsI 816 GAAGAC GAAGAC(2/6)
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
BciVI 838 GTATCC GTATCC(6/5)
BfrI 775 CTTAAG C^TTAAG
BglII 575 AGATCT A^GATCT
BpuAI 816 GAAGAC GAAGAC(2/6)
BseRI 523 GAGGAG GAGGAG(10/8)
BsgI 1427 CTGCAC GTGCAG(16/14)
BsiHKAI 541 GAGCTC G[AT]GC[AT]^C
BsmI 1422 GAATGC GAATGC(1/-1)
BspMI 624 GCAGGT ACCTGC(4/8)
BsrBI 1338 CCGCTC CCGCTC(-3/-3)
BsrFI 1306 GCCGGC [GA]^CCGG[CT]
CfoI 708 GCGC GCG^C
Cfr10I 1306 GCCGGC [GA]^CCGG[CT]
DsaI 821 CCATGG C^C[GA][CT]GG
EcoNI 963 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 707 AGCGCC [GA]GCGC^[CT]
HhaI 708 GCGC GCG^C
HinP1I 708 GCGC G^CGC
HindIII 149 AAGCTT A^AGCTT
NaeI 1306 GCCGGC GCC^GGC
NcoI 821 CCATGG C^CATGG
NgoMIV 1306 GCCGGC G^CCGGC
PvuII 47 CAGCTG CAG^CTG
SacI 541 GAGCTC GAGCT^C
SalI 1082 GTCGAC G^TCGAC
ScaI 881 AGTACT AGT^ACT
SpeI 552 ACTAGT A^CTAGT
XmnI 592 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AocI 891 CCTAAGG CC^T[ACGT]AGG
1076 CCTCAGG
BglI 721 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1154 GCCGAAAGGGC
BstAPI 146 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
624 GCAGGTGTTGC
BstXI 842 CCATAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCAACAGATTGG
Bsu36I 891 CCTAAGG CC^T[ACGT]AGG
1076 CCTCAGG
Eco81I 891 CCTAAGG CC^T[ACGT]AGG
1076 CCTCAGG
HgaI 983 GCGTC GACGC(5/10)
1052 GCGTC
HincII 1082 GTCGAC GT[CT]^[GA]AC
1167 GTTGAC
HindII 1082 GTCGAC GT[CT]^[GA]AC
1167 GTTGAC
MseI 776 TTAA T^TAA
905 TTAA
MspA1I 47 CAGCTG C[AC]G^C[GT]G
162 CCGCTG
NlaIII 331 CATG CATG^
822 CATG
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
713 GGGACCT
SapI 592 GAAGAGC GCTCTTC(1/4)
616 GAAGAGC
Tru9I 776 TTAA T^TAA
905 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII