(Circular) (Six-base) MAPSORT of: pFN21AB8733.seq Check: 3741 from: 1 to: 6169 pFN21AB8733 6169 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 6169 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3088 3512 1937 Size: 1151 424 4594 Fragments arranged by size: 4594 1151 424 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 2823 2851 4232 5616 5884 1632 Size: 500 691 28 1381 1384 268 1917 Fragments arranged by size: 1917 1384 1381 691 500 268 28 AflII C'TTAA_G Cuts at: 820 1017 2780 820 Size: 197 1763 4209 Fragments arranged by size: 4209 1763 197 AflIII A'CryG_T Cuts at: 1203 5068 6045 1203 Size: 3865 977 1327 Fragments arranged by size: 3865 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 2423 1166 Size: 1257 4912 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2497 3912 5484 2497 Size: 1415 1572 3182 Fragments arranged by size: 3182 1572 1415 ApaI G_GGCC'C Cuts at: 2416 2416 Size: 6169 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 3494 1870 Size: 73 1551 4545 Fragments arranged by size: 4545 1551 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2826 2859 2826 Size: 33 6136 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3490 4945 618 Size: 325 217 627 1703 1455 1842 Fragments arranged by size: 1842 1703 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2416 2484 2550 3488 1391 Size: 1025 68 66 938 4072 Fragments arranged by size: 4072 1025 938 68 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2828 928 Size: 971 929 4269 Fragments arranged by size: 4269 971 929 BbvCI CC'TCA_GC Cuts at: 2345 2345 Size: 6169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2854 4061 5271 2854 Size: 1207 1210 3752 Fragments arranged by size: 3752 1210 1207 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 3165 4780 Size: 107 122 71 631 605 1060 433 1615 Cuts at: 4780 136 Size: 1525 Fragments arranged by size: 1615 1525 1060 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 2580 6164 2580 Size: 3584 2585 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2142 2416 4230 5386 1790 Size: 352 274 1814 1156 2573 Fragments arranged by size: 2573 1814 1156 352 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3106 3136 3900 Size: 416 53 83 399 24 1689 30 764 Cuts at: 3900 4858 442 Size: 958 1753 Fragments arranged by size: 1753 1689 958 764 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2270 2498 4829 1239 Size: 884 147 228 2331 2579 Fragments arranged by size: 2579 2331 884 228 147 Bpu10I CC'TnA_GC Cuts at: 2345 2345 Size: 6169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 2605 4294 5159 5457 2534 Size: 71 1689 865 298 3246 Fragments arranged by size: 3246 1689 865 298 71 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3960 493 Size: 711 2756 2702 Fragments arranged by size: 2756 2702 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 4358 4993 275 Size: 53 83 186 936 2443 382 635 1451 Fragments arranged by size: 2443 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2738 2768 1178 Size: 30 165 30 1335 30 4579 Fragments arranged by size: 4579 1335 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 4812 BseYI C'CCAG_C Cuts at: 2030 5372 5868 2030 Size: 3342 496 2331 Fragments arranged by size: 3342 2331 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3417 1163 Size: 573 1681 3915 Fragments arranged by size: 3915 1681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2550 4230 4315 5386 2550 Size: 1680 85 1071 3333 Fragments arranged by size: 3333 1680 1071 85 BsmI GAATG_Cn' Cuts at: 3433 3680 3773 3433 Size: 247 93 5829 Fragments arranged by size: 5829 247 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2416 2484 2550 3488 4230 4315 Size: 399 352 274 68 66 938 742 85 Cuts at: 4315 5386 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 938 742 399 352 274 85 68 66 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 3524 844 Size: 1776 904 3489 Fragments arranged by size: 3489 1776 904 BsrBI CCG'CTC Cuts at: 1394 1823 3345 4056 1394 Size: 429 1522 711 3507 Fragments arranged by size: 3507 1522 711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3311 4813 5000 1700 Size: 110 1501 1502 187 2869 Fragments arranged by size: 2869 1502 1501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 4229 5241 4229 Size: 1012 5157 BstAPI GCAn_nnn'nTGC Cuts at: 794 2157 2635 794 Size: 1363 478 4328 Fragments arranged by size: 4328 1363 478 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2854 3462 2854 Size: 608 5561 BstYI r'GATC_y Cuts at: 1972 2304 2580 3016 3499 3866 4251 4268 5720 Size: 332 276 436 483 367 385 17 1452 Cuts at: 5720 5806 5818 6164 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 483 436 385 367 346 332 276 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2897 3082 2897 Size: 185 5984 BtgI C'CryG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 3856 Fragments arranged by size: 3856 939 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 3138 3685 4479 4499 3138 Size: 547 794 20 4808 Fragments arranged by size: 4808 794 547 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3616 4505 6134 Size: 54 1425 50 275 18 1786 889 1629 Cuts at: 6134 8 Size: 43 Fragments arranged by size: 1786 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 2616 2649 3401 4099 1353 Size: 1239 24 33 752 698 3423 Fragments arranged by size: 3423 1239 752 698 33 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2548 2548 Size: 6169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2132 2270 2498 2823 2851 4232 Size: 393 491 9 138 228 325 28 1381 Cuts at: 4232 4829 5616 5884 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1381 787 597 491 393 325 268 228 138 28 9 EcoNI CCTnn'n_nnAGG Cuts at: 2972 2972 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2034 2179 2341 2719 3450 2034 Size: 145 162 378 731 4753 Fragments arranged by size: 4753 731 378 162 145 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 2716 5316 1678 Size: 1038 2600 2531 Fragments arranged by size: 2600 2531 1038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2565 2597 2938 2970 4810 Size: 32 135 32 993 32 341 32 1840 Cuts at: 4810 4842 4884 4916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1840 993 341 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3089 3174 3513 3759 669 Size: 988 281 1151 85 339 246 3079 Fragments arranged by size: 3079 1151 988 339 281 246 85 HindIII A'AGCT_T Cuts at: 748 2154 748 Size: 1406 4763 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 5283 5467 1305 Size: 958 3020 184 2007 Fragments arranged by size: 3020 2007 958 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4347 Fragments arranged by size: 4347 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2831 3269 4127 4486 4645 518 Size: 648 1257 408 438 858 359 159 2042 Fragments arranged by size: 2042 1257 858 648 438 408 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2169 3585 4262 5410 5655 1606 Size: 448 115 1416 677 1148 245 2120 Fragments arranged by size: 2120 1416 1148 677 448 245 115 NaeI GCC'GGC Cuts at: 3313 3313 Size: 6169 NcoI C'CATG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 3856 Fragments arranged by size: 3856 939 822 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NgoMIV G'CCGG_C Cuts at: 3311 3311 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PfoI T'CCnGG_A Cuts at: 2307 3012 2307 Size: 705 5464 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2719 2179 Size: 540 5629 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2412 2412 Size: 6169 PstI C_TGCA'G Cuts at: 830 2044 2469 2502 2676 3521 830 Size: 1214 425 33 174 845 3478 Fragments arranged by size: 3478 1214 845 425 174 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2054 2054 Size: 6169 SacI G_AGCT'C Cuts at: 2550 2550 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3087 3511 1936 Size: 1151 424 4594 Fragments arranged by size: 4594 1151 424 SapI GCTCTTCn'nnn_ Cuts at: 2592 2616 2592 Size: 24 6145 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 2888 4417 6129 2888 Size: 1529 1712 2928 Fragments arranged by size: 2928 1712 1529 SfcI C'TryA_G Cuts at: 826 1045 2040 2049 2465 2498 2672 3517 4652 Size: 219 995 9 416 33 174 845 1135 Cuts at: 4652 5333 5524 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 995 845 681 416 219 191 174 33 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2549 2584 2780 4273 5174 5436 Size: 197 926 606 35 196 1493 901 262 Cuts at: 5436 820 Size: 1553 Fragments arranged by size: 1553 1493 926 901 606 262 197 196 35 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 2557 152 Size: 2405 3764 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2004 2701 2826 3032 3608 513 Size: 552 939 697 125 206 576 3074 Fragments arranged by size: 3074 939 697 576 552 206 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 3125 4196 4213 4366 4551 4996 Size: 1255 840 705 1071 17 153 185 445 Cuts at: 4996 325 Size: 1498 Fragments arranged by size: 1498 1255 1071 840 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 2886 4415 Size: 274 80 33 51 1443 474 435 1529 Cuts at: 4415 6127 96 Size: 1712 138 Fragments arranged by size: 1712 1529 1443 474 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2738 2770 6129 6161 2738 Size: 32 3359 32 2746 Fragments arranged by size: 3359 2746 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 2601 4298 2601 Size: 1697 4472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI