Restriction map for
pFN21AB8733
(Linear) (Six-base) MAPSORT of: KIBB8733.seq Check: 2801 from: 1 to: 1464
KIBB8733.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 1464
Size: 615 849
AccI GT'mk_AC
Cuts at: 0 1083 1464
Size: 1083 381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 818 846 1464
Size: 127 691 28 618
Fragments arranged by size:
691 618 127 28
AflII C'TTAA_G
Cuts at: 0 775 1464
Size: 775 689
AleI CACnn'nnGTG
Cuts at: 0 418 1464
Size: 418 1046
AlwNI CAG_nnn'CTG
Cuts at: 0 492 1464
Size: 492 972
ApaI G_GGCC'C
Cuts at: 0 411 1464
Size: 411 1053
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 821 854 1464
Size: 821 33 610
Fragments arranged by size:
821 610 33
BanII G_rGCy'C
Cuts at: 0 411 479 545 1464
Size: 411 68 66 919
Fragments arranged by size:
919 411 68 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 823 1464
Size: 823 641
BbvCI CC'TCA_GC
Cuts at: 0 340 1464
Size: 340 1124
BciVI GTATCCnnnnn_n'
Cuts at: 0 849 1464
Size: 849 615
BglI GCCn_nnn'nGGC
Cuts at: 0 727 1160 1464
Size: 727 433 304
Fragments arranged by size:
727 433 304
BglII A'GATC_T
Cuts at: 0 575 1464
Size: 575 889
Bme1580I G_kGCm'C
Cuts at: 0 137 411 1464
Size: 137 274 1053
Fragments arranged by size:
1053 274 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 1101 1131 1464
Size: 1101 30 333
Fragments arranged by size:
1101 333 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 265 493 1464
Size: 118 147 228 971
Fragments arranged by size:
971 228 147 118
Bpu10I CC'TnA_GC
Cuts at: 0 340 1464
Size: 340 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 600 1464
Size: 529 71 864
Fragments arranged by size:
864 529 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 733 763 1464
Size: 733 30 701
Fragments arranged by size:
733 701 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1464
Size: 538 926
BseYI C'CCAG_C
Cuts at: 0 25 1464
Size: 25 1439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1464
Size: 1412 52
BsiHKAI G_wGCw'C
Cuts at: 0 545 1464
Size: 545 919
BsmI GAATG_Cn'
Cuts at: 0 1428 1464
Size: 1428 36
Bsp1286I G_dGCh'C
Cuts at: 0 137 411 479 545 1464
Size: 137 274 68 66 919
Fragments arranged by size:
919 274 137 68 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 1464
Size: 615 849
BsrBI CCG'CTC
Cuts at: 0 1340 1464
Size: 1340 124
BsrFI r'CCGG_y
Cuts at: 0 1306 1464
Size: 1306 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 152 630 1464
Size: 152 478 834
Fragments arranged by size:
834 478 152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 849 1457 1464
Size: 849 608 7
Fragments arranged by size:
849 608 7
BstYI r'GATC_y
Cuts at: 0 299 575 1011 1464
Size: 299 276 436 453
Fragments arranged by size:
453 436 299 276
Bsu36I CC'TnA_GG
Cuts at: 0 892 1077 1464
Size: 892 185 387
Fragments arranged by size:
892 387 185
BtgI C'CryG_G
Cuts at: 0 821 1464
Size: 821 643
BtsI GCAGTG_nn'
Cuts at: 0 1133 1464
Size: 1133 331
EarI CTCTTCn'nnn_
Cuts at: 0 587 611 644 1396 1464
Size: 587 24 33 752 68
Fragments arranged by size:
752 587 68 33 24
EcoICRI GAG'CTC
Cuts at: 0 543 1464
Size: 543 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 127 265 493 818 846 1464
Size: 118 9 138 228 325 28 618
Fragments arranged by size:
618 325 228 138 118 28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 967 1464
Size: 967 497
EcoO109I rG'GnC_Cy
Cuts at: 0 29 174 336 714 1445 1464
Size: 29 145 162 378 731 19
Fragments arranged by size:
731 378 162 145 29 19
HaeII r_GCGC'y
Cuts at: 0 711 1464
Size: 711 753
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 560 592 933 965 1464
Size: 560 32 341 32 499
Fragments arranged by size:
560 499 341 32 32
HincII GTy'rAC
Cuts at: 0 1084 1169 1464
Size: 1084 85 295
Fragments arranged by size:
1084 295 85
HindIII A'AGCT_T
Cuts at: 0 149 1464
Size: 149 1315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1464
Size: 258 1206
MslI CAynn'nnrTG
Cuts at: 0 418 826 1264 1464
Size: 418 408 438 200
Fragments arranged by size:
438 418 408 200
MspA1I CmG'CkG
Cuts at: 0 49 164 1464
Size: 49 115 1300
Fragments arranged by size:
1300 115 49
NaeI GCC'GGC
Cuts at: 0 1308 1464
Size: 1308 156
NcoI C'CATG_G
Cuts at: 0 821 1464
Size: 821 643
NgoMIV G'CCGG_C
Cuts at: 0 1306 1464
Size: 1306 158
PfoI T'CCnGG_A
Cuts at: 0 302 1007 1464
Size: 302 705 457
Fragments arranged by size:
705 457 302
PpuMI rG'GwC_Cy
Cuts at: 0 174 714 1464
Size: 174 540 750
Fragments arranged by size:
750 540 174
PspOMI G'GGCC_C
Cuts at: 0 407 1464
Size: 407 1057
PstI C_TGCA'G
Cuts at: 0 39 464 497 671 1464
Size: 39 425 33 174 793
Fragments arranged by size:
793 425 174 39 33
PvuII CAG'CTG
Cuts at: 0 49 1464
Size: 49 1415
SacI G_AGCT'C
Cuts at: 0 545 1464
Size: 545 919
SalI G'TCGA_C
Cuts at: 0 1082 1464
Size: 1082 382
SapI GCTCTTCn'nnn_
Cuts at: 0 587 611 1464
Size: 587 24 853
Fragments arranged by size:
853 587 24
ScaI AGT'ACT
Cuts at: 0 883 1464
Size: 883 581
SfcI C'TryA_G
Cuts at: 0 35 44 460 493 667 1464
Size: 35 9 416 33 174 797
Fragments arranged by size:
797 416 174 35 33 9
SmlI C'TyrA_G
Cuts at: 0 544 579 775 1464
Size: 544 35 196 689
Fragments arranged by size:
689 544 196 35
SpeI A'CTAG_T
Cuts at: 0 552 1464
Size: 552 912
StyI C'CwwG_G
Cuts at: 0 696 821 1027 1464
Size: 696 125 206 437
Fragments arranged by size:
696 437 206 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 1120 1464
Size: 415 705 344
Fragments arranged by size:
705 415 344
TatI w'GTAC_w
Cuts at: 0 446 881 1464
Size: 446 435 583
Fragments arranged by size:
583 446 435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 733 765 1464
Size: 733 32 699
Fragments arranged by size:
733 699 32
XmnI GAAnn'nnTTC
Cuts at: 0 596 1464
Size: 596 868
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlwNI ApaI BaeI
BanII BbsI BbvCI BciVI BglI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII MmeI MslI MspA1I NaeI
NcoI NgoMIV PfoI PpuMI PspOMI PstI PvuII SacI
SalI SapI ScaI SfcI SmlI SpeI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BcgI BclI BfrBI BlpI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI