(Circular) (Six-base) MAPSORT of: pFN21AE1015.seq Check: 9508 from: 1 to: 5695 pFN21AE1015 5695 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 3758 5142 5410 1632 Size: 857 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 857 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2050 4594 5571 1203 Size: 847 2544 977 1327 Fragments arranged by size: 2544 1327 977 847 AgeI A'CCGG_T Cuts at: 2565 4526 2565 Size: 1961 3734 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 3438 5010 3438 Size: 1572 4123 ApaI G_GGCC'C Cuts at: 2929 2929 Size: 5695 ApaLI G'TGCA_C Cuts at: 2236 3752 4908 2236 Size: 1516 1156 3023 Fragments arranged by size: 3023 1516 1156 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 3020 1870 Size: 73 1077 4545 Fragments arranged by size: 4545 1077 73 BamHI G'GATC_C Cuts at: 2040 2673 2940 3025 3392 2040 Size: 633 267 85 367 4343 Fragments arranged by size: 4343 633 367 267 85 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2899 3016 4471 618 Size: 325 217 627 357 755 117 1455 1842 Fragments arranged by size: 1842 1455 755 627 357 325 217 117 BanII G_rGCy'C Cuts at: 1391 2079 2170 2190 2483 2929 3014 1391 Size: 688 91 20 293 446 85 4072 Fragments arranged by size: 4072 688 446 293 91 85 20 BbeI G_GCGC'C Cuts at: 2903 2903 Size: 5695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2603 2637 3886 3920 1903 Size: 34 666 34 1249 34 3678 Fragments arranged by size: 3678 1249 666 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 2852 2933 Size: 107 122 71 631 605 1159 21 81 Cuts at: 2933 4306 136 Size: 1373 1525 Fragments arranged by size: 1525 1373 1159 631 605 122 107 81 71 21 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2147 2240 2929 3756 4912 1790 Size: 357 93 689 827 1156 2573 Fragments arranged by size: 2573 1156 827 689 357 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2257 3426 4384 Size: 416 53 83 399 24 840 1169 958 Cuts at: 4384 442 Size: 1753 Fragments arranged by size: 1753 1169 958 840 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2900 2951 4355 1239 Size: 1130 531 51 1404 2579 Fragments arranged by size: 2579 1404 1130 531 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2615 3820 4685 4983 2615 Size: 1205 865 298 3327 Fragments arranged by size: 3327 1205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 4358 882 Size: 617 720 2139 2219 Fragments arranged by size: 2219 2139 720 617 BsaAI yAC'GTr Cuts at: 493 1204 2490 3486 493 Size: 711 1286 996 2702 Fragments arranged by size: 2702 1286 996 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 2900 3502 3884 Size: 53 83 186 936 888 479 602 382 Cuts at: 3884 4519 275 Size: 635 1451 Fragments arranged by size: 1451 936 888 635 602 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2565 4128 4526 4800 4947 5358 2118 Size: 447 1563 398 274 147 411 2455 Fragments arranged by size: 2455 1563 447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2753 1186 Size: 899 668 4128 Fragments arranged by size: 4128 899 668 BseYI C'CCAG_C Cuts at: 2439 2890 4898 5394 2439 Size: 451 2008 496 2740 Fragments arranged by size: 2740 2008 496 451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 1163 Size: 573 375 4747 Fragments arranged by size: 4747 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2835 3145 3906 4934 1540 Size: 275 186 834 310 761 1028 2301 Fragments arranged by size: 2301 1028 834 761 310 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2190 2240 3756 3841 4912 2079 Size: 111 50 1516 85 1071 2862 Fragments arranged by size: 2862 1516 1071 111 85 50 BsmI GAATG_Cn' Cuts at: 2848 3206 3299 2848 Size: 358 93 5244 Fragments arranged by size: 5244 358 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2147 2170 2190 2240 2483 2929 Size: 399 289 68 23 20 50 243 446 Cuts at: 2929 3014 3756 3841 4912 1391 Size: 85 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 289 243 85 85 68 50 23 20 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2646 3050 844 Size: 1802 404 3489 Fragments arranged by size: 3489 1802 404 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2619 4190 4364 65 Size: 1183 1371 1571 174 1396 Fragments arranged by size: 1571 1396 1371 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2565 4339 4526 1700 Size: 110 755 1774 187 2869 Fragments arranged by size: 2869 1774 755 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 2169 3755 4767 2169 Size: 1586 1012 3097 Fragments arranged by size: 3097 1586 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2878 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2040 2673 2940 2982 3025 3392 3777 3794 Size: 68 633 267 42 43 367 385 17 Cuts at: 3794 5246 5332 5344 5690 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 633 385 367 346 267 86 68 43 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2504 513 Size: 552 939 177 323 3704 Fragments arranged by size: 3704 939 552 323 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2002 2311 487 Size: 683 56 776 309 3871 Fragments arranged by size: 3871 776 683 309 56 BtsI GCAGTG_nn' Cuts at: 2362 2544 2761 3211 4005 4025 2362 Size: 182 217 450 794 20 4032 Fragments arranged by size: 4032 794 450 217 182 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2795 4702 809 Size: 507 1479 1907 1802 Fragments arranged by size: 1907 1802 1479 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 2832 3142 Size: 54 1425 50 275 18 477 525 310 Cuts at: 3142 4031 5660 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 525 477 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2832 3142 1537 Size: 275 1020 310 4090 Fragments arranged by size: 4090 1020 310 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2517 2773 2952 3625 1353 Size: 1164 256 179 673 3423 Fragments arranged by size: 3423 1164 673 256 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4282 4656 4802 387 Size: 1064 138 435 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2188 2077 Size: 111 5584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2489 2900 2951 3758 4355 5142 Size: 393 737 120 411 51 807 597 787 Cuts at: 5142 5410 1239 Size: 268 1524 Fragments arranged by size: 1524 807 787 737 597 411 393 268 120 51 EcoO109I rG'GnC_Cy Cuts at: 2341 2500 2661 2926 2341 Size: 159 161 265 5110 Fragments arranged by size: 5110 265 161 159 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 EcoRV GAT'ATC Cuts at: 2014 2014 Size: 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 2824 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2903 4842 1678 Size: 1225 1939 2531 Fragments arranged by size: 2531 1939 1225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 4336 4368 4410 Size: 32 135 32 1178 32 1554 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 1178 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2899 2899 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 10 Size: 54 1425 343 477 3396 Fragments arranged by size: 3396 1425 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2330 2462 3653 4012 4171 518 Size: 648 1164 132 1191 359 159 2042 Fragments arranged by size: 2042 1191 1164 648 359 159 132 MspA1I CmG'CkG Cuts at: 1606 2302 2359 2680 2701 3111 3788 4936 5181 Size: 696 57 321 21 410 677 1148 245 Cuts at: 5181 1606 Size: 2120 Fragments arranged by size: 2120 1148 696 677 410 321 245 57 21 NarI GG'CG_CC Cuts at: 2900 2900 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 2004 2181 2504 513 Size: 552 939 177 323 3704 Fragments arranged by size: 3704 939 552 323 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2288 2588 3053 4598 1132 Size: 97 1059 300 465 1545 2229 Fragments arranged by size: 2229 1545 1059 465 300 97 PasI CC'CwG_GG Cuts at: 1348 2401 1348 Size: 1053 4642 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PfoI T'CCnGG_A Cuts at: 2028 2079 2028 Size: 51 5644 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2500 2661 2341 Size: 159 161 5375 Fragments arranged by size: 5375 161 159 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2925 2925 Size: 5695 PstI C_TGCA'G Cuts at: 830 2430 2643 3047 830 Size: 1600 213 404 3478 Fragments arranged by size: 3478 1600 404 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2680 2680 Size: 5695 SacI G_AGCT'C Cuts at: 2079 2190 2079 Size: 111 5584 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SanDI GG'GwC_CC Cuts at: 2661 2661 Size: 5695 SapI GCTCTTCn'nnn_ Cuts at: 2773 2773 Size: 5695 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 2609 2744 3943 5655 2609 Size: 135 1199 1712 2649 Fragments arranged by size: 2649 1712 1199 135 SfcI C'TryA_G Cuts at: 826 1045 2426 2639 3043 4178 4859 5050 826 Size: 219 1381 213 404 1135 681 191 1471 Fragments arranged by size: 1471 1381 1135 681 404 219 213 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2831 2831 Size: 5695 SfoI GGC'GCC Cuts at: 2901 2901 Size: 5695 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2630 3799 4700 4962 820 Size: 197 926 687 1169 901 262 1553 Fragments arranged by size: 1553 1169 926 901 687 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2804 1517 Size: 1287 4408 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2181 2504 3134 513 Size: 552 939 96 81 323 630 3074 Fragments arranged by size: 3074 939 630 552 323 96 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 2742 3941 Size: 274 80 33 51 1443 630 135 1199 Cuts at: 3941 5653 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1199 630 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 5655 5687 2753 Size: 32 2870 32 2761 Fragments arranged by size: 2870 2761 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2795 1316 Size: 1479 4216 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI XcmI