Restriction map for
pFN21AB5422
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbsI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NarI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 346 GAATTC [GA]^AATT[CT]
AcyI 530 GGCGTC G[GA]^CG[CT]C
AlwI 321 GGATC GGATC(4/5)
ApaI 626 GGGCCC GGGCC^C
ApoI 346 GAATTC [GA]^AATT[CT]
BbrPI 58 CACGTG CAC^GTG
BcgI 615 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 206 AGATCT A^GATCT
BsaAI 58 CACGTG [CT]AC^GT[GA]
BsaHI 530 GGCGTC G[GA]^CG[CT]C
BsrFI 581 GCCGGC [GA]^CCGG[CT]
BssSI 127 CTCGTG CACGAG(-5/-1)
BstEII 601 GGTCACC G^GT[ACGT]ACC
BstPI 601 GGTCACC G^GT[ACGT]ACC
Cfr10I 581 GCCGGC [GA]^CCGG[CT]
DsaI 191 CCGTGG C^C[GA][CT]GG
EaeI 194 TGGCCG [CT]^GGCC[GA]
EcoNI 153 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 601 GGTCACC G^GT[ACGT]ACC
EcoRI 346 GAATTC G^AATTC
HgaI 531 GCGTC GACGC(5/10)
Hin1I 530 GGCGTC G[GA]^CG[CT]C
MaeII 59 ACGT A^CGT
MslI 54 CACGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 581 GCCGGC GCC^GGC
NgoMIV 581 GCCGGC G^CCGGC
PaeR7I 329 CTCGAG C^TCGAG
PflMI 186 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 58 CACGTG CAC^GTG
PmlI 58 CACGTG CAC^GTG
PstI 99 CTGCAG CTGCA^G
SapI 475 GAAGAGC GCTCTTC(1/4)
SexAI 359 ACCAGGT A^CC[AT]GGT
SfcI 99 CTGCAG C^T[GA][CT]AG
SmlI 329 CTCGAG C^T[CT][GA]AG
Tsp509I 347 AATT ^AATT
TspRI 171 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 186 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 329 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 424 CGCG CG^CG
430 CGCG
AfaI 394 GTAC GT^AC
589 GTAC
Asp700I 204 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
342 GAAGGAATTC
BanII 626 GGGCCC G[GA]GC[CT]^C
652 GAGCCC
BcnI 457 CCCGG CC^[GC]GG
527 CCCGG
BpmI 43 CTGGAG CTGGAG(16/14)
279 CTGGAG
BsaI 332 GAGACC GGTCTC(1/5)
499 GGTCTC
BsaJI 191 CCGTGG C^C[ACGT][ACGT]GG
596 CCGAGG
BsgI 229 CTGCAC GTGCAG(16/14)
244 CTGCAC
BsmAI 332 GAGAC GTCTC(1/5)
500 GTCTC
BstUI 424 CGCG CG^CG
430 CGCG
BstYI 206 AGATCT [GA]^GATC[CT]
321 GGATCT
DdeI 173 CTGAG C^T[ACGT]AG
650 CTGAG
DpnII 207 GATC ^GATC
322 GATC
EarI 262 GAAGAG CTCTTC(1/4)
475 GAAGAG
HphI 603 TCACC GGTGA(8/7)
618 TCACC
Ksp632I 262 GAAGAG CTCTTC(1/4)
475 GAAGAG
MaeIII 130 GTGAC ^GT[ACGT]AC
602 GTCAC
MboI 207 GATC ^GATC
322 GATC
MflI 206 AGATCT [GA]^GATC[CT]
321 GGATCT
MvnI 424 CGCG CG^CG
430 CGCG
NciI 457 CCCGG CC^[GC]GG
527 CCCGG
NdeII 207 GATC ^GATC
322 GATC
PpuMI 113 GGGACCT [GA]G^G[AT]CC[CT]
440 GGGACCT
PvuII 73 CAGCTG CAG^CTG
398 CAGCTG
RsaI 394 GTAC GT^AC
589 GTAC
Sau3AI 207 GATC ^GATC
322 GATC
SfaNI 482 GATGC GCATC(5/9)
615 GCATC
Tsp45I 130 GTGAC ^GT[GC]AC
602 GTCAC
XhoII 206 AGATCT [GA]^GATC[CT]
321 GGATCT
XmnI 204 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
342 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwNI AvaI AvaII
BbvI BmyI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MwoI NlaIV Sau96I ScrFI
TaqI TseI TthHB8I