Restriction map for
pFN21AB5422
(Circular) (Six-base) MAPSORT of: pFN21AB5422.seq Check: 970 from: 1 to: 5377
pFN21AB5422 5377 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3440 4824 5092 1632
Size: 654 1154 1384 268 1917
Fragments arranged by size:
1917 1384 1154 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 2063 1166
Size: 897 4480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2174 1184
Size: 990 4387
AlwNI CAG_nnn'CTG
Cuts at: 2083 2112 2420 3120 4692 2083
Size: 29 308 700 1572 2768
Fragments arranged by size:
2768 1572 700 308 29
ApaI G_GGCC'C
Cuts at: 2635 2635
Size: 5377
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2351 2686 2912 3064 5173 1493
Size: 858 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 858 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2334 2600 2702 1870
Size: 73 90 301 266 102 4545
Fragments arranged by size:
4545 301 266 102 90 73
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 4153 618
Size: 325 217 627 911 1455 1842
Fragments arranged by size:
1842 1455 911 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2635 2661 2696 1391
Size: 1244 26 35 4072
Fragments arranged by size:
4072 1244 35 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2609 2643 3568 3602 1903
Size: 34 672 34 925 34 3678
Fragments arranged by size:
3678 925 672 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3988 136
Size: 107 122 71 631 605 2316 1525
Fragments arranged by size:
2316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2211 5372 2211
Size: 3161 2216
BlpI GC'TnA_GC
Cuts at: 2794 2794
Size: 5377
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2635 3438 4594 1790
Size: 280 565 803 1156 2573
Fragments arranged by size:
2573 1156 803 565 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 2591 3108
Size: 416 53 83 399 24 777 397 517
Cuts at: 3108 4066 442
Size: 958 1753
Fragments arranged by size:
1753 958 777 517 416 399 397 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2305 4037 1239
Size: 830 236 1732 2579
Fragments arranged by size:
2579 1732 830 236
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2331 2510 4040 882
Size: 617 832 179 1530 2219
Fragments arranged by size:
2219 1530 832 617 179
BsaAI yAC'GTr
Cuts at: 493 1204 2065 3168 493
Size: 711 861 1103 2702
Fragments arranged by size:
2702 1103 861 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2536 3184 3566 4201
Size: 53 83 186 936 1003 648 382 635
Cuts at: 4201 275
Size: 1451
Fragments arranged by size:
1451 1003 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3810 4208 4482 4629 5040 3810
Size: 398 274 147 411 4147
Fragments arranged by size:
4147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2502 2532 1178
Size: 30 165 30 1099 30 4023
Fragments arranged by size:
4023 1099 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5377
BseYI C'CCAG_C
Cuts at: 4580 5076 4580
Size: 496 4881
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2234 1163
Size: 573 483 15 4306
Fragments arranged by size:
4306 573 483 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3438 3523 4594 3438
Size: 85 1071 4221
Fragments arranged by size:
4221 1071 85
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2635 2661 2696 3438 3523 4594
Size: 399 280 565 26 35 742 85 1071
Cuts at: 4594 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 565 399 280 85 35
26
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 844
Size: 1888 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3264 1394
Size: 429 1441 3507
Fragments arranged by size:
3507 1441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2586 4021 4208 1700
Size: 110 776 1435 187 2869
Fragments arranged by size:
2869 1435 776 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 2132 3437 4449 2132
Size: 1305 1012 3060
Fragments arranged by size:
3060 1305 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 2606 1184
Size: 1422 3955
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5377
BstYI r'GATC_y
Cuts at: 1972 2211 2326 2707 3074 3459 3476 4928 5014
Size: 239 115 381 367 385 17 1452 86
Cuts at: 5014 5026 5372 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 381 367 346 239 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2196 513
Size: 552 939 192 3694
Fragments arranged by size:
3694 939 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 2500 487
Size: 683 56 1096 178 3364
Fragments arranged by size:
3364 1096 683 178 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2824 3713
Size: 54 1425 50 275 18 369 625 889
Cuts at: 3713 5342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 625 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2475 3307 1353
Size: 909 213 832 3423
Fragments arranged by size:
3423 909 832 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2286 2305 3440 4037 4824 5092
Size: 393 437 217 19 1135 597 787 268
Cuts at: 5092 1239
Size: 1524
Fragments arranged by size:
1524 1135 787 597 437 393 268 217
19
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2162
Size: 5377
EcoO109I rG'GnC_Cy
Cuts at: 2119 2446 2632 2119
Size: 327 186 4864
Fragments arranged by size:
4864 327 186
EcoRI G'AATT_C
Cuts at: 2351 2686 3064 2351
Size: 335 378 4664
Fragments arranged by size:
4664 378 335
FspI TGC'GCA
Cuts at: 3883 3883
Size: 5377
HaeII r_GCGC'y
Cuts at: 1678 4524 1678
Size: 2846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 2648 2680 4018
Size: 32 135 32 804 32 240 32 1338
Cuts at: 4018 4050 4092 4124 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1338 804 240 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2545 4491 4675 1305
Size: 1240 1946 184 2007
Fragments arranged by size:
2007 1946 1240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2063 3335 3694 3853 518
Size: 648 843 54 1272 359 159 2042
Fragments arranged by size:
2042 1272 843 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2080 2405 2639 2793 3470 4618 4863 1606
Size: 474 325 234 154 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 474 325 245 234 154
NaeI GCC'GGC
Cuts at: 2588 2588
Size: 5377
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3886
Fragments arranged by size:
3886 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NgoMIV G'CCGG_C
Cuts at: 2586 2586
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2735 4280 1132
Size: 97 1506 1545 2229
Fragments arranged by size:
2229 1545 1506 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5377
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 1108
Size: 1089 4288
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PmlI CAC'GTG
Cuts at: 2065 2065
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2446 2119
Size: 327 5050
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2631 2631
Size: 5377
PstI C_TGCA'G
Cuts at: 830 2108 2729 830
Size: 1278 621 3478
Fragments arranged by size:
3478 1278 621
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
PvuII CAG'CTG
Cuts at: 2080 2405 2080
Size: 325 5052
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SapI GCTCTTCn'nnn_
Cuts at: 2475 2475
Size: 5377
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SexAI A'CCwGG_T
Cuts at: 2364 2364
Size: 5377
SfcI C'TryA_G
Cuts at: 826 1045 2104 2725 3860 4541 4732 826
Size: 219 1059 621 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 681 621 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2334 3481 4382 4644 820
Size: 197 926 391 1147 901 262 1553
Fragments arranged by size:
1553 1147 926 901 391 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2735 1132
Size: 1603 3774
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5377
StyI C'CwwG_G
Cuts at: 513 1065 2004 2816 513
Size: 552 939 812 3074
Fragments arranged by size:
3074 939 812 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 5337 5369 2502
Size: 32 2803 32 2510
Fragments arranged by size:
2803 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 2334 1943
Size: 391 4986
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 2213 2351 3506 2213
Size: 138 1155 4084
Fragments arranged by size:
4084 1155 138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI