Restriction map for
pFN21AB5361
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApaI
ApoI AscI AseI AsnI AspI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaAI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KasI
Ksp632I KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI Tsp45I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 78 enzymes) #
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Acc65I 32 GGTACC G^GTACC
AccII 107 CGCG CG^CG
AccIII 744 TCCGGA T^CCGGA
AcyI 825 GACGCC G[GA]^CG[CT]C
AfaI 33 GTAC GT^AC
AhdI 1263 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 178 GTGCAC G^TGCAC
ApaLI 178 GTGCAC G^TGCAC
Asp718I 32 GGTACC G^GTACC
AspEI 1263 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 178 GTGCAC G[AT]GC[AT]^C
AvaI 901 CCCGGG C^[CT]CG[GA]G
BamHI 817 GGATCC G^GATCC
BanI 32 GGTACC G^G[CT][GA]CC
BbsI 502 GAAGAC GAAGAC(2/6)
BfaI 619 CTAG C^TAG
BglI 991 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 633 AGATCT A^GATCT
BmyI 178 GTGCAC G[AGT]GC[ACT]^C
BpmI 61 CTGGAG CTGGAG(16/14)
BpuAI 502 GAAGAC GAAGAC(2/6)
BsaBI 628 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 825 GACGCC G[GA]^CG[CT]C
BsaI 295 GAGACC GGTCTC(1/5)
BsaWI 744 TCCGGA [AT]^CCGG[AT]
BseAI 744 TCCGGA T^CCGGA
BseRI 64 GAGGAG GAGGAG(10/8)
BsgI 83 GTGCAG GTGCAG(16/14)
BsiHKAI 178 GTGCAC G[AT]GC[AT]^C
BsmI 143 GCATTC GAATGC(1/-1)
BsoBI 901 CCCGGG C^[CT]CG[GA]G
Bsp1286I 178 GTGCAC G[AGT]GC[ACT]^C
BspEI 744 TCCGGA T^CCGGA
BspMI 53 ACCTGC ACCTGC(4/8)
BsrBI 863 CCGCTC CCGCTC(-3/-3)
BsrFI 712 GCCGGC [GA]^CCGG[CT]
BstAPI 703 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 107 CGCG CG^CG
BstXI 1071 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 544 GCGC GCG^C
Cfr10I 712 GCCGGC [GA]^CCGG[CT]
DrdI 3 GACAGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 995 CCATGG C^C[GA][CT]GG
Eam1105I 1263 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 618 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 995 CCATGG C^C[AT][AT]GG
FauI 862 CCCGC CCCGC(4/6)
HaeII 543 AGCGCC [GA]GCGC^[CT]
HgaI 825 GACGC GACGC(5/10)
HhaI 544 GCGC GCG^C
Hin1I 825 GACGCC G[GA]^CG[CT]C
HinP1I 544 GCGC G^CGC
HincII 202 GTTAAC GT[CT]^[GA]AC
HindII 202 GTTAAC GT[CT]^[GA]AC
HpaI 202 GTTAAC GTT^AAC
KpnI 32 GGTACC GGTAC^C
MaeI 619 CTAG C^TAG
MaeII 23 ACGT A^CGT
MaeIII 907 GTTAC ^GT[ACGT]AC
MamI 628 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 744 TCCGGA T^CCGGA
MseI 203 TTAA T^TAA
MvnI 107 CGCG CG^CG
NaeI 712 GCCGGC GCC^GGC
NcoI 995 CCATGG C^CATGG
NgoMIV 712 GCCGGC G^CCGGC
PflMI 1170 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 373 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 33 GTAC GT^AC
SbfI 1006 CCTGCAGG CCTGCA^GG
SmaI 901 CCCGGG CCC^GGG
Sse8387I 1006 CCTGCAGG CCTGCA^GG
StyI 995 CCATGG C^C[AT][AT]GG
Tru9I 203 TTAA T^TAA
Tsp509I 455 AATT ^AATT
Van91I 1170 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 901 CCCGGG C^CCGGG
XmaI 901 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 542 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1138 CAGCAGCTG
Asp700I 226 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
436 GAACCAGTTC
AvaII 372 GGACC G^G[AT]CC
1125 GGTCC
BalI 47 TGGCCA TGG^CCA
998 TGGCCA
BcnI 901 CCCGG CC^[GC]GG
902 CCGGG
BlpI 964 GCTCAGC GC^T[ACGT]AGC
1135 GCTCAGC
Bpu1102I 964 GCTCAGC GC^T[ACGT]AGC
1135 GCTCAGC
BsmAI 295 GAGAC GTCTC(1/5)
344 GAGAC
BstYI 633 AGATCT [GA]^GATC[CT]
817 GGATCC
CelII 964 GCTCAGC GC^T[ACGT]AGC
1135 GCTCAGC
EaeI 47 TGGCCA [CT]^GGCC[GA]
998 TGGCCA
HphI 15 GGTGA GGTGA(8/7)
480 GGTGA
MflI 633 AGATCT [GA]^GATC[CT]
817 GGATCC
MluNI 47 TGGCCA TGG^CCA
998 TGGCCA
MscI 47 TGGCCA TGG^CCA
998 TGGCCA
MslI 126 CATTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATGGCCATG
MspA1I 323 CAGCTG C[AC]G^C[GT]G
1141 CAGCTG
NciI 901 CCCGG CC^[GC]GG
902 CCGGG
NspI 239 GCATGC [GA]CATG^[CT]
1025 GCATGC
PstI 701 CTGCAG CTGCA^G
1007 CTGCAG
PvuII 323 CAGCTG CAG^CTG
1141 CAGCTG
SfcI 701 CTGCAG C^T[GA][CT]AG
1007 CTGCAG
SmlI 221 CTCAAG C^T[CT][GA]AG
842 CTCAAG
SphI 239 GCATGC GCATG^C
1025 GCATGC
XhoII 633 AGATCT [GA]^GATC[CT]
817 GGATCC
XmnI 226 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
436 GAACCAGTTC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI TspRI TthHB8I XcmI