Restriction map for
pFN21AB5361
(Circular) (Six-base) MAPSORT of: pFN21AB5361.seq Check: 5078 from: 1 to: 5992
pFN21AB5361 5992 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 2037 3313 2037
Size: 1276 4716
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4055 5439 5707 1632
Size: 2423 1384 268 1917
Fragments arranged by size:
2423 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 3273 4721 3273
Size: 1448 4544
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2552 3148 3735 5307 2552
Size: 596 587 1572 3237
Fragments arranged by size:
3237 1572 596 587
ApaLI G'TGCA_C
Cuts at: 2183 4049 5205 2183
Size: 1866 1156 2970
Fragments arranged by size:
2970 1866 1156
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 3317 1870
Size: 73 963 411 4545
Fragments arranged by size:
4545 963 411 73
BamHI G'GATC_C
Cuts at: 2822 3322 3689 2822
Size: 500 367 5125
Fragments arranged by size:
5125 500 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 3313 4768 618
Size: 325 217 627 250 1276 1455 1842
Fragments arranged by size:
1842 1455 1276 627 325 250 217
BanII G_rGCy'C
Cuts at: 1391 3311 1391
Size: 1920 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 928
Size: 971 615 4406
Fragments arranged by size:
4406 971 615
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 4603 136
Size: 107 122 71 631 605 1330 1601 1525
Fragments arranged by size:
1601 1525 1330 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2638 5987 2638
Size: 3349 2643
BlpI GC'TnA_GC
Cuts at: 2970 3141 3409 2970
Size: 171 268 5553
Fragments arranged by size:
5553 268 171
Bme1580I G_kGCm'C
Cuts at: 1790 2187 4053 5209 1790
Size: 397 1866 1156 2573
Fragments arranged by size:
2573 1866 1156 397
BmgBI CAC'GTC
Cuts at: 1318 2029 1318
Size: 711 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 4652 1239
Size: 848 2565 2579
Fragments arranged by size:
2579 2565 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 2832 4117 4982 5280 2211
Size: 621 1285 865 298 2923
Fragments arranged by size:
2923 1285 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 4655 882
Size: 617 795 2361 2219
Fragments arranged by size:
2361 2219 795 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 2637 5986 2637
Size: 3349 2643
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2831 3799 4181 4816
Size: 53 83 186 936 1298 968 382 635
Cuts at: 4816 275
Size: 1451
Fragments arranged by size:
1451 1298 968 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2749 4425 4823 5097 5244 5655 2749
Size: 1676 398 274 147 411 3086
Fragments arranged by size:
3086 1676 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5767
Fragments arranged by size:
5767 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 1186
Size: 898 5094
BseYI C'CCAG_C
Cuts at: 2326 2897 3075 3174 3185 5195 5691 2326
Size: 571 178 99 11 2010 496 2627
Fragments arranged by size:
2627 2010 571 496 178 99 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 1163
Size: 573 373 5046
Fragments arranged by size:
5046 573 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2187 4053 4138 5209 2187
Size: 1866 85 1071 2970
Fragments arranged by size:
2970 1866 1071 85
BsmI GAATG_Cn'
Cuts at: 2148 3503 3596 2148
Size: 1355 93 4544
Fragments arranged by size:
4544 1355 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 3311 4053 4138 5209 1391
Size: 399 397 1124 742 85 1071 2174
Fragments arranged by size:
2174 1124 1071 742 399 397 85
BspEI T'CCGG_A
Cuts at: 2749 5655 2749
Size: 2906 3086
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3347 844
Size: 1223 1280 3489
Fragments arranged by size:
3489 1280 1223
BsrBI CCG'CTC
Cuts at: 1394 1823 2870 3879 1394
Size: 429 1047 1009 3507
Fragments arranged by size:
3507 1047 1009 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2595 2859 3158 4487 4661 65
Size: 1183 1347 264 299 1329 174 1396
Fragments arranged by size:
1396 1347 1329 1183 299 264 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2717 4636 4823 1700
Size: 110 907 1919 187 2869
Fragments arranged by size:
2869 1919 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2714 794
Size: 1920 4072
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 3083 3083
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2638 2822 3322 3689 4074 4091 5543 5629
Size: 666 184 500 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 500 385 367 346 184
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3000 513
Size: 552 1935 3505
Fragments arranged by size:
3505 1935 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 487
Size: 683 56 902 4351
Fragments arranged by size:
4351 902 683 56
BtsI GCAGTG_nn'
Cuts at: 3508 4302 4322 3508
Size: 794 20 5178
Fragments arranged by size:
5178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 4999 809
Size: 507 698 2985 1802
Fragments arranged by size:
2985 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 3003 3439
Size: 54 1425 50 275 18 222 951 436
Cuts at: 3439 4328 5957 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 951 889 436 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3922 1353
Size: 2569 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3258 4579 4953 5099 387
Size: 1064 138 1669 1321 374 146 1280
Fragments arranged by size:
1669 1321 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 4055 4652 5439 5707 1239
Size: 393 455 1968 597 787 268 1524
Fragments arranged by size:
1968 1524 787 597 455 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 2627
Size: 5992
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2309 2309
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2552 5139 1678
Size: 874 2587 2531
Fragments arranged by size:
2587 2531 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2866 2898 3259
Size: 32 135 32 512 32 750 32 361
Cuts at: 3259 3291 4633 4665 4707 4739 1373
Size: 32 1342 32 42 32 2626
Fragments arranged by size:
2626 1342 750 512 361 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 3336 3582 669
Size: 988 281 271 1127 246 3079
Fragments arranged by size:
3079 1127 988 281 271 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 2209 3582 2209
Size: 1373 4619
KpnI G_GTAC'C
Cuts at: 2041 3317 2041
Size: 1276 4716
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5106 5290 1305
Size: 3801 184 2007
Fragments arranged by size:
3801 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 3005 10
Size: 54 1425 343 222 951 2997
Fragments arranged by size:
2997 1425 951 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3005 3950 4309 4468 518
Size: 648 969 870 945 359 159 2042
Fragments arranged by size:
2042 969 945 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 3148 3408 4085 5233 5478 1606
Size: 724 818 260 677 1148 245 2120
Fragments arranged by size:
2120 1148 818 724 677 260 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 5992
NcoI C'CATG_G
Cuts at: 513 1065 3000 513
Size: 552 1935 3505
Fragments arranged by size:
3505 1935 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2248 3034 3350 4895 1132
Size: 97 1019 786 316 1545 2229
Fragments arranged by size:
2229 1545 1019 786 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3181 1108
Size: 2073 3919
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2382 1613
Size: 769 5223
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2710 3016 3344 830
Size: 1880 306 328 3478
Fragments arranged by size:
3478 1880 328 306
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2330 3148 2330
Size: 818 5174
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SbfI CC_TGCA'GG
Cuts at: 3016 3344 3016
Size: 328 5664
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 2706 3012 3340 4475 5156 5347 826
Size: 219 1661 306 328 1135 681 191 1471
Fragments arranged by size:
1661 1471 1135 681 328 306 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2908 3319 1872
Size: 1036 411 4545
Fragments arranged by size:
4545 1036 411
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 2847 4096 4997 5259 820
Size: 197 926 283 621 1249 901 262 1553
Fragments arranged by size:
1553 1249 926 901 621 283 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 2248 3034 3350 1132
Size: 1116 786 316 3774
Fragments arranged by size:
3774 1116 786 316
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 3000 3431 513
Size: 552 1935 431 3074
Fragments arranged by size:
3074 1935 552 431
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2394 3014 3038 3089 4019 4036 4189
Size: 1255 814 620 24 51 930 17 153
Cuts at: 4189 4374 4819 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 930 814 620 445 185 153
51 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2119 2151 5952 5984 2119
Size: 32 3801 32 2127
Fragments arranged by size:
3801 2127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 3122 3182 2062
Size: 1060 60 4872
Fragments arranged by size:
4872 1060 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 2906 3317 1870
Size: 1036 411 4545
Fragments arranged by size:
4545 1036 411
XmnI GAAnn'nnTTC
Cuts at: 2235 2445 4121 2235
Size: 210 1676 4106
Fragments arranged by size:
4106 1676 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI