Restriction map for
pFN21AB5246
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbsI BbvCI
BcgI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoT22I FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluNI MroI MscI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 179 ACGCGT A^C[GA][CT]GT
AlwNI 1268 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 205 GGCGCGCC GG^CGCGCC
BbrPI 658 CACGTG CAC^GTG
BciVI 1151 GTATCC GTATCC(6/5)
BclI 1409 TGATCA T^GATCA
BfaI 605 CTAG C^TAG
BglI 242 GCCCGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 658 CACGTG [CT]AC^GT[GA]
BseRI 807 CTCCTC GAGGAG(10/8)
BsgI 424 CTGCAC GTGCAG(16/14)
BspHI 1406 TCATGA T^CATGA
BstEII 663 GGTGACC G^GT[ACGT]ACC
BstPI 663 GGTGACC G^GT[ACGT]ACC
DraII 239 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 658 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 20 CTCTTC CTCTTC(1/4)
EcoNI 231 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 239 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 663 GGTGACC G^GT[ACGT]ACC
EcoRV 1402 GATATC GAT^ATC
EcoT14I 468 CCAAGG C^C[AT][AT]GG
FbaI 1409 TGATCA T^GATCA
HindIII 571 AAGCTT A^AGCTT
Ksp632I 20 CTCTTC CTCTTC(1/4)
MaeI 605 CTAG C^TAG
MluI 179 ACGCGT A^CGCGT
PleI 1096 GAGTC GAGTC(4/5)
PmaCI 658 CACGTG CAC^GTG
PmlI 658 CACGTG CAC^GTG
RcaI 1406 TCATGA T^CATGA
SapI 19 GCTCTTC GCTCTTC(1/4)
SbfI 231 CCTGCAGG CCTGCA^GG
SfaNI 1307 GCATC GCATC(5/9)
SmlI 1282 CTCAAG C^T[CT][GA]AG
Sse8387I 231 CCTGCAGG CCTGCA^GG
StyI 468 CCAAGG C^C[AT][AT]GG
TaqI 557 TCGA T^CGA
TthHB8I 557 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 1294 GACGTC GACGT^C
1348 GACGTC
ApaI 170 GGGCCC GGGCC^C
240 GGGCCC
Asp700I 559 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAAGTAATTC
AspHI 151 GAGCAC G[AT]GC[AT]^C
891 GAGCAC
AvaII 1197 GGACC G^G[AT]CC
1383 GGTCC
BsaI 1279 GGTCTC GGTCTC(1/5)
1326 GGTCTC
BsiHKAI 151 GAGCAC G[AT]GC[AT]^C
891 GAGCAC
BsmAI 1280 GTCTC GTCTC(1/5)
1327 GTCTC
BstNI 774 CCAGG CC^[AT]GG
1002 CCTGG
BstYI 5 AGATCC [GA]^GATC[CT]
480 AGATCC
EcoRII 774 CCAGG ^CC[AT]GG
1002 CCTGG
FseI 78 GGCCGGCC GGCCGG^CC
318 GGCCGGCC
MflI 5 AGATCC [GA]^GATC[CT]
480 AGATCC
MvaI 774 CCAGG CC^[AT]GG
1002 CCTGG
SmaI 74 CCCGGG CCC^GGG
173 CCCGGG
TfiI 1049 GAATC G^A[AT]TC
1419 GATTC
XhoII 5 AGATCC [GA]^GATC[CT]
480 AGATCC
XmaCI 74 CCCGGG C^CCGGG
173 CCCGGG
XmaI 74 CCCGGG C^CCGGG
173 CCCGGG
XmnI 559 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAAGTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BssHII BssKI BstF5I
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PstI
PvuII Sau3AI Sau96I ScrFI SfcI
SfoI Tru9I TseI Tsp45I Tsp509I
TspRI