(Circular) (Six-base) MAPSORT of: pFN21AB5246.seq Check: 9529 from: 1 to: 6145 pFN21AB5246 6145 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3303 3357 3955 278 Size: 53 83 186 936 1767 54 598 2468 Fragments arranged by size: 2468 1767 936 598 186 83 54 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2720 3218 4208 5592 5860 1632 Size: 1088 498 990 1384 268 1917 Fragments arranged by size: 1917 1384 1088 990 498 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 2184 5044 6021 1203 Size: 981 2860 977 1327 Fragments arranged by size: 2860 1327 981 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 3278 3888 5460 3278 Size: 610 1572 3963 Fragments arranged by size: 3963 1572 610 ApaI G_GGCC'C Cuts at: 2179 2249 2179 Size: 70 6075 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AscI GG'CGCG_CC Cuts at: 2211 2211 Size: 6145 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2178 2216 2986 3470 1870 Size: 73 136 99 38 770 484 4545 Fragments arranged by size: 4545 770 484 136 99 73 38 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2095 2112 2222 2275 Size: 325 217 627 269 39 17 110 53 Cuts at: 2275 2287 2599 2708 3207 3466 4921 618 Size: 12 312 109 499 259 1455 1842 Fragments arranged by size: 1842 1455 627 499 325 312 269 259 217 110 109 53 39 17 12 BanII G_rGCy'C Cuts at: 1391 2179 2249 2931 3464 1391 Size: 788 70 682 533 4072 Fragments arranged by size: 4072 788 682 533 70 BbeI G_GCGC'C Cuts at: 2060 2099 2226 2060 Size: 39 127 5979 Fragments arranged by size: 5979 127 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4336 4370 1903 Size: 34 50 34 2315 34 3678 Fragments arranged by size: 3678 2315 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3167 4037 5247 3167 Size: 870 1210 4065 Fragments arranged by size: 4065 1210 870 BclI T'GATC_A Cuts at: 1548 3414 1548 Size: 1866 4279 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 4756 136 Size: 107 122 71 631 605 581 2503 1525 Fragments arranged by size: 2503 1525 631 605 581 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2117 2179 2249 2280 4206 5362 1790 Size: 327 62 70 31 1926 1156 2573 Fragments arranged by size: 2573 1926 1156 327 70 62 31 BmgBI CAC'GTC Cuts at: 1318 2527 3177 3226 1318 Size: 1209 650 49 4237 Fragments arranged by size: 4237 1209 650 49 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3437 3876 4834 Size: 416 53 83 399 24 2020 439 958 Cuts at: 4834 442 Size: 1753 Fragments arranged by size: 2020 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4805 1239 Size: 3566 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3272 4270 5135 5433 3272 Size: 998 865 298 3984 Fragments arranged by size: 3984 998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3290 3337 4808 882 Size: 617 1791 47 1471 2219 Fragments arranged by size: 2219 1791 1471 617 47 BsaAI yAC'GTr Cuts at: 493 1204 2665 3936 493 Size: 711 1461 1271 2702 Fragments arranged by size: 2702 1461 1271 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2096 2223 2370 Size: 53 83 186 936 524 39 127 147 Cuts at: 2370 2964 3300 3354 3952 4334 4969 275 Size: 594 336 54 598 382 635 1451 Fragments arranged by size: 1451 936 635 598 594 524 382 336 186 147 127 83 54 53 39 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 2823 2853 1178 Size: 30 165 30 831 30 559 30 4470 Fragments arranged by size: 4470 831 559 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2803 1186 Size: 1617 4528 BseYI C'CCAG_C Cuts at: 2904 2923 3268 5348 5844 2904 Size: 19 345 2080 496 3205 Fragments arranged by size: 3205 2080 496 345 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 1163 Size: 573 678 4894 Fragments arranged by size: 4894 678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2089 2173 2438 2828 3595 4356 Size: 275 186 88 84 265 390 767 761 Cuts at: 4356 5384 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 767 761 390 275 265 186 88 84 BsiHKAI G_wGCw'C Cuts at: 2160 2900 4206 4291 5362 2160 Size: 740 1306 85 1071 2943 Fragments arranged by size: 2943 1306 1071 740 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2160 2179 2249 2280 2900 2931 Size: 399 327 43 19 70 31 620 31 Cuts at: 2931 3464 4206 4291 5362 1391 Size: 533 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 533 399 327 85 70 43 31 31 19 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 3411 4034 5764 3411 Size: 623 1730 3792 Fragments arranged by size: 3792 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 844 Size: 2656 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2024 2091 2418 2811 4032 1394 Size: 429 201 67 327 393 1221 3507 Fragments arranged by size: 3507 1221 429 393 327 201 67 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2131 2324 2353 2433 2440 2602 Size: 110 274 47 193 29 80 7 162 Cuts at: 2602 3204 4789 4976 1700 Size: 602 1585 187 2869 Fragments arranged by size: 2869 1585 602 274 193 187 162 110 80 47 29 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 2017 2135 2211 2314 2316 2401 1925 Size: 92 118 76 103 2 85 5669 Fragments arranged by size: 5669 118 103 92 85 76 2 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 2668 1184 Size: 1484 4661 BstYI r'GATC_y Cuts at: 1972 2010 2485 3475 3842 4227 4244 5696 5782 Size: 38 475 990 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 990 475 385 367 346 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2301 2550 3327 513 Size: 552 1236 249 777 3331 Fragments arranged by size: 3331 1236 777 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2740 487 Size: 683 56 1514 3892 Fragments arranged by size: 3892 1514 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2273 3661 4455 4475 2158 Size: 115 1388 794 20 3828 Fragments arranged by size: 3828 1388 794 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DraIII CAC_nnn'GTG Cuts at: 2668 2668 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2103 2170 Size: 54 1425 50 275 18 256 17 67 Cuts at: 2170 2298 2435 2470 2806 2825 3592 4481 6110 Size: 128 137 35 336 19 767 889 1629 Cuts at: 6110 8 Size: 43 Fragments arranged by size: 1629 1425 889 767 336 275 256 137 128 67 54 50 43 35 19 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2086 2170 2435 2825 3592 1537 Size: 275 274 84 265 390 767 4090 Fragments arranged by size: 4090 767 390 275 274 265 84 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 4075 1353 Size: 678 2044 3423 Fragments arranged by size: 3423 2044 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3325 4732 5106 5252 387 Size: 1064 138 1736 1407 374 146 1280 Fragments arranged by size: 1736 1407 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2720 3218 4208 4805 5592 5860 1239 Size: 393 1088 498 990 597 787 268 1524 Fragments arranged by size: 1524 1088 990 787 597 498 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2245 2245 Size: 6145 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 EcoRV GAT'ATC Cuts at: 3409 3409 Size: 6145 FseI GG_CCGG'CC Cuts at: 2088 2328 2088 Size: 240 5905 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2060 2099 2226 5292 1678 Size: 382 39 127 3066 2531 Fragments arranged by size: 3066 2531 382 127 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3215 3247 4786 4818 4860 Size: 32 135 32 1643 32 1539 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2626 1643 1539 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 2576 748 Size: 1828 4317 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KasI G'GCGC_C Cuts at: 2056 2095 2222 2056 Size: 39 127 5979 Fragments arranged by size: 5979 127 39 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 2184 6021 2184 Size: 3837 2308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 5259 5443 1305 Size: 1308 2646 184 2007 Fragments arranged by size: 2646 2007 1308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4103 4462 4621 518 Size: 648 2937 359 159 2042 Fragments arranged by size: 2937 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2074 2124 2398 3050 3561 4238 5386 Size: 429 39 50 274 652 511 677 1148 Cuts at: 5386 5631 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 652 511 429 274 245 50 39 NaeI GCC'GGC Cuts at: 2086 2133 2326 2086 Size: 47 193 5905 Fragments arranged by size: 5905 193 47 NarI GG'CG_CC Cuts at: 2057 2096 2223 2057 Size: 39 127 5979 Fragments arranged by size: 5979 127 39 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NgoMIV G'CCGG_C Cuts at: 2084 2131 2324 2084 Size: 47 193 5905 Fragments arranged by size: 5905 193 47 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3503 5048 1132 Size: 97 2274 1545 2229 Fragments arranged by size: 2274 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 2665 2665 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2175 2245 2175 Size: 70 6075 PstI C_TGCA'G Cuts at: 830 2241 2268 2457 3497 830 Size: 1411 27 189 1040 3478 Fragments arranged by size: 3478 1411 1040 189 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2074 2398 3050 2074 Size: 324 652 5169 Fragments arranged by size: 5169 652 324 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SapI GCTCTTCn'nnn_ Cuts at: 2031 2031 Size: 6145 SbfI CC_TGCA'GG Cuts at: 2241 3497 2241 Size: 1256 4889 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2237 2264 2453 3493 4628 5309 5500 Size: 219 1192 27 189 1040 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1192 1135 1040 681 219 191 189 27 SfoI GGC'GCC Cuts at: 2058 2097 2224 2058 Size: 39 127 5979 Fragments arranged by size: 5979 127 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2081 2180 3472 1872 Size: 209 99 1292 4545 Fragments arranged by size: 4545 1292 209 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4249 5150 5412 820 Size: 197 926 1344 962 901 262 1553 Fragments arranged by size: 1553 1344 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2473 3584 513 Size: 552 1408 1111 3074 Fragments arranged by size: 3074 1408 1111 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 2975 3126 4172 4189 4342 4527 Size: 1255 1338 57 151 1046 17 153 185 Cuts at: 4527 4972 325 Size: 445 1498 Fragments arranged by size: 1498 1338 1255 1046 445 185 153 151 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4391 6103 96 Size: 274 80 33 51 1443 2414 1712 138 Fragments arranged by size: 2414 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 2634 2666 2823 2855 3312 3344 3409 Size: 32 368 32 157 32 457 32 65 Cuts at: 3409 3441 6105 6137 2234 Size: 32 2664 32 2242 Fragments arranged by size: 2664 2242 457 368 157 65 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2079 2178 3470 1870 Size: 209 99 1292 4545 Fragments arranged by size: 4545 1292 209 99 XmnI GAAnn'nnTTC Cuts at: 2568 3255 4274 2568 Size: 687 1019 4439 Fragments arranged by size: 4439 1019 687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3301 3355 3953 276 Size: 53 83 186 936 1767 54 598 2468 Fragments arranged by size: 2468 1767 936 598 186 83 54 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI