(Linear) (Six-base) MAPSORT of: KIBB5246.seq Check: 266 from: 1 to: 1440 KIBB5246.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AatII G_ACGT'C Cuts at: 0 1298 1352 1440 Size: 1298 54 88 Fragments arranged by size: 1298 88 54 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 1213 1440 Size: 715 498 227 Fragments arranged by size: 715 498 227 AflIII A'CryG_T Cuts at: 0 179 1440 Size: 179 1261 AlwNI CAG_nnn'CTG Cuts at: 0 1273 1440 Size: 1273 167 ApaI G_GGCC'C Cuts at: 0 174 244 1440 Size: 174 70 1196 Fragments arranged by size: 1196 174 70 AscI GG'CGCG_CC Cuts at: 0 206 1440 Size: 206 1234 AvaI C'yCGr_G Cuts at: 0 74 173 211 981 1440 Size: 74 99 38 770 459 Fragments arranged by size: 770 459 99 74 38 BanI G'GyrC_C Cuts at: 0 51 90 107 217 270 282 594 703 Size: 51 39 17 110 53 12 312 109 Cuts at: 703 1202 1440 Size: 499 238 Fragments arranged by size: 499 312 238 110 109 53 51 39 17 12 BanII G_rGCy'C Cuts at: 0 174 244 926 1440 Size: 174 70 682 514 Fragments arranged by size: 682 514 174 70 BbeI G_GCGC'C Cuts at: 0 55 94 221 1440 Size: 55 39 127 1219 Fragments arranged by size: 1219 127 55 39 BciVI GTATCCnnnnn_n' Cuts at: 0 1162 1440 Size: 1162 278 BclI T'GATC_A Cuts at: 0 1409 1440 Size: 1409 31 BglI GCCn_nnn'nGGC Cuts at: 0 248 1440 Size: 248 1192 Bme1580I G_kGCm'C Cuts at: 0 112 174 244 275 1440 Size: 112 62 70 31 1165 Fragments arranged by size: 1165 112 70 62 31 BmgBI CAC'GTC Cuts at: 0 522 1172 1221 1440 Size: 522 650 49 219 Fragments arranged by size: 650 522 219 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1267 1440 Size: 1267 173 BsaI GGTCTCn'nnnn_ Cuts at: 0 1285 1332 1440 Size: 1285 47 108 Fragments arranged by size: 1285 108 47 BsaAI yAC'GTr Cuts at: 0 660 1440 Size: 660 780 BsaHI Gr'CG_yC Cuts at: 0 52 91 218 365 959 1295 1349 1440 Size: 52 39 127 147 594 336 54 91 Fragments arranged by size: 594 336 147 127 91 54 52 39 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 229 259 818 848 1440 Size: 229 30 559 30 592 Fragments arranged by size: 592 559 229 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 798 1440 Size: 798 642 BseYI C'CCAG_C Cuts at: 0 899 918 1263 1440 Size: 899 19 345 177 Fragments arranged by size: 899 345 177 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 1440 Size: 409 1031 BsiEI CG_ry'CG Cuts at: 0 84 168 433 823 1440 Size: 84 84 265 390 617 Fragments arranged by size: 617 390 265 84 84 BsiHKAI G_wGCw'C Cuts at: 0 155 895 1440 Size: 155 740 545 Fragments arranged by size: 740 545 155 Bsp1286I G_dGCh'C Cuts at: 0 112 155 174 244 275 895 926 1440 Size: 112 43 19 70 31 620 31 514 Fragments arranged by size: 620 514 112 70 43 31 31 19 BspHI T'CATG_A Cuts at: 0 1406 1440 Size: 1406 34 BsrBI CCG'CTC Cuts at: 0 19 86 413 806 1440 Size: 19 67 327 393 634 Fragments arranged by size: 634 393 327 67 19 BsrFI r'CCGG_y Cuts at: 0 79 126 319 348 428 435 597 1199 Size: 79 47 193 29 80 7 162 602 Cuts at: 1199 1440 Size: 241 Fragments arranged by size: 602 241 193 162 80 79 47 29 7 BssHII G'CGCG_C Cuts at: 0 12 130 206 309 311 396 1440 Size: 12 118 76 103 2 85 1044 Fragments arranged by size: 1044 118 103 85 76 12 2 BstEII G'GTnAC_C Cuts at: 0 663 1440 Size: 663 777 BstYI r'GATC_y Cuts at: 0 5 480 1440 Size: 5 475 960 Fragments arranged by size: 960 475 5 BtgI C'CryG_G Cuts at: 0 296 545 1322 1440 Size: 296 249 777 118 Fragments arranged by size: 777 296 249 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 735 1440 Size: 735 705 BtsI GCAGTG_nn' Cuts at: 0 153 268 1440 Size: 153 115 1172 Fragments arranged by size: 1172 153 115 DraIII CAC_nnn'GTG Cuts at: 0 663 1440 Size: 663 777 EaeI y'GGCC_r Cuts at: 0 81 98 165 293 430 465 801 820 Size: 81 17 67 128 137 35 336 19 Cuts at: 820 1440 Size: 620 Fragments arranged by size: 620 336 137 128 81 67 35 19 17 EagI C'GGCC_G Cuts at: 0 81 165 430 820 1440 Size: 81 84 265 390 620 Fragments arranged by size: 620 390 265 84 81 EarI CTCTTCn'nnn_ Cuts at: 0 26 1440 Size: 26 1414 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1320 1440 Size: 1320 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 1213 1440 Size: 715 498 227 Fragments arranged by size: 715 498 227 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 1440 Size: 235 1205 EcoO109I rG'GnC_Cy Cuts at: 0 240 1440 Size: 240 1200 EcoRV GAT'ATC Cuts at: 0 1404 1440 Size: 1404 36 FseI GG_CCGG'CC Cuts at: 0 83 323 1440 Size: 83 240 1117 Fragments arranged by size: 1117 240 83 HaeII r_GCGC'y Cuts at: 0 55 94 221 1440 Size: 55 39 127 1219 Fragments arranged by size: 1219 127 55 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1210 1242 1440 Size: 1210 32 198 Fragments arranged by size: 1210 198 32 HindIII A'AGCT_T Cuts at: 0 571 1440 Size: 571 869 KasI G'GCGC_C Cuts at: 0 51 90 217 1440 Size: 51 39 127 1223 Fragments arranged by size: 1223 127 51 39 MluI A'CGCG_T Cuts at: 0 179 1440 Size: 179 1261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 608 1440 Size: 608 832 MspA1I CmG'CkG Cuts at: 0 30 69 119 393 1045 1440 Size: 30 39 50 274 652 395 Fragments arranged by size: 652 395 274 50 39 30 NaeI GCC'GGC Cuts at: 0 81 128 321 1440 Size: 81 47 193 1119 Fragments arranged by size: 1119 193 81 47 NarI GG'CG_CC Cuts at: 0 52 91 218 1440 Size: 52 39 127 1222 Fragments arranged by size: 1222 127 52 39 NgoMIV G'CCGG_C Cuts at: 0 79 126 319 1440 Size: 79 47 193 1121 Fragments arranged by size: 1121 193 79 47 PmlI CAC'GTG Cuts at: 0 660 1440 Size: 660 780 PspOMI G'GGCC_C Cuts at: 0 170 240 1440 Size: 170 70 1200 Fragments arranged by size: 1200 170 70 PstI C_TGCA'G Cuts at: 0 236 263 452 1440 Size: 236 27 189 988 Fragments arranged by size: 988 236 189 27 PvuII CAG'CTG Cuts at: 0 69 393 1045 1440 Size: 69 324 652 395 Fragments arranged by size: 652 395 324 69 SapI GCTCTTCn'nnn_ Cuts at: 0 26 1440 Size: 26 1414 SbfI CC_TGCA'GG Cuts at: 0 236 1440 Size: 236 1204 SfcI C'TryA_G Cuts at: 0 232 259 448 1440 Size: 232 27 189 992 Fragments arranged by size: 992 232 189 27 SfoI GGC'GCC Cuts at: 0 53 92 219 1440 Size: 53 39 127 1221 Fragments arranged by size: 1221 127 53 39 SmaI CCC'GGG Cuts at: 0 76 175 1440 Size: 76 99 1265 Fragments arranged by size: 1265 99 76 SmlI C'TyrA_G Cuts at: 0 1282 1440 Size: 1282 158 StyI C'CwwG_G Cuts at: 0 468 1440 Size: 468 972 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 913 970 1121 1440 Size: 913 57 151 319 Fragments arranged by size: 913 319 151 57 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 229 261 629 661 818 850 1307 1339 Size: 229 32 368 32 157 32 457 32 Cuts at: 1339 1404 1436 1440 Size: 65 32 4 Fragments arranged by size: 457 368 229 157 65 32 32 32 32 32 4 XmaI C'CCGG_G Cuts at: 0 74 173 1440 Size: 74 99 1267 Fragments arranged by size: 1267 99 74 XmnI GAAnn'nnTTC Cuts at: 0 563 1250 1440 Size: 563 687 190 Fragments arranged by size: 687 563 190 ZraI GAC'GTC Cuts at: 0 1296 1350 1440 Size: 1296 54 90 Fragments arranged by size: 1296 90 54 Enzymes that do cut: AatII AcuI AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI BciVI BclI BglI Bme1580I BmgBI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssHII BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FseI HaeII Hin4I HindIII KasI MluI MmeI MspA1I NaeI NarI NgoMIV PmlI PspOMI PstI PvuII SapI SbfI SfcI SfoI SmaI SmlI StyI TaqII TstI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BfrBI BglII BlpI BmrI BmtI BplI BpmI Bpu10I BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRI FalI FspI FspAI HincII HpaI KpnI MfeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI