################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccI AclI AflIII AgeI AocI AscI AseI AsnI Asp700I AspI AviII AvrII BbeI BciVI BlnI BsaI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV EcoT22I FspI HincII HindII HpaI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 878 GGTACC G^GTACC AccIII 166 TCCGGA T^CCGGA AflII 1402 CTTAAG C^TTAAG AhdI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 809 AGCGCT AGC^GCT Asp718I 878 GGTACC G^GTACC AspEI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2165 TGGCCA TGG^CCA BbrPI 844 CACGTG CAC^GTG BbvCI 2008 CCTCAGC CCTCAGC(-5/-2) BcgI 310 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 905 TGATCA T^GATCA BfrI 1402 CTTAAG C^TTAAG BglII 1370 AGATCT A^GATCT BsaAI 844 CACGTG [CT]AC^GT[GA] BsaBI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 166 TCCGGA [AT]^CCGG[AT] BseAI 166 TCCGGA T^CCGGA BsgI 1240 GTGCAG GTGCAG(16/14) BspEI 166 TCCGGA T^CCGGA BspMI 2664 ACCTGC ACCTGC(4/8) BstEII 2673 GGTGACC G^GT[ACGT]ACC BstPI 2673 GGTGACC G^GT[ACGT]ACC DraIII 1318 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 809 AGCGCT AGC^GCT EcoNI 675 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2673 GGTGACC G^GT[ACGT]ACC FbaI 905 TGATCA T^GATCA FseI 1014 GGCCGGCC GGCCGG^CC HaeII 809 AGCGCT [GA]GCGC^[CT] HindIII 1558 AAGCTT A^AGCTT KpnI 878 GGTACC GGTAC^C MamI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2165 TGGCCA TGG^CCA MroI 166 TCCGGA T^CCGGA MscI 2165 TGGCCA TGG^CCA NheI 508 GCTAGC G^CTAGC NspI 1907 ACATGC [GA]CATG^[CT] PmaCI 844 CACGTG CAC^GTG PmlI 844 CACGTG CAC^GTG PpuMI 2751 AGGACCT [GA]G^G[AT]CC[CT] PshAI 598 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2379 GAGCTC GAGCT^C SapI 130 GAAGAGC GCTCTTC(1/4) SexAI 1421 ACCAGGT A^CC[AT]GGT SgrAI 989 CGCCGGTG C[GA]^CCGG[CT]G SmaI 2568 CCCGGG CCC^GGG XmaCI 2568 CCCGGG C^CCGGG XmaI 2568 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 1859 AAATTT [GA]^AATT[CT] 2505 GAATTT AcyI 40 GACGCC G[GA]^CG[CT]C 2215 GACGCC Alw44I 1426 GTGCAC G^TGCAC 2356 GTGCAC ApaLI 1426 GTGCAC G^TGCAC 2356 GTGCAC ApoI 1859 AAATTT [GA]^AATT[CT] 2505 GAATTT BamHI 950 GGATCC G^GATCC 1397 GGATCC BbsI 969 GAAGAC GAAGAC(2/6) 1839 GAAGAC BfaI 227 CTAG C^TAG 509 CTAG BlpI 2381 GCTCAGC GC^T[ACGT]AGC 2562 GCTGAGC Bpu1102I 2381 GCTCAGC GC^T[ACGT]AGC 2562 GCTGAGC BpuAI 969 GAAGAC GAAGAC(2/6) 1839 GAAGAC BsaHI 40 GACGCC G[GA]^CG[CT]C 2215 GACGCC CelII 2381 GCTCAGC GC^T[ACGT]AGC 2562 GCTGAGC Hin1I 40 GACGCC G[GA]^CG[CT]C 2215 GACGCC MaeI 227 CTAG C^TAG 509 CTAG MaeII 845 ACGT A^CGT 1976 ACGT PstI 1155 CTGCAG CTGCA^G 2082 CTGCAG SfcI 1155 CTGCAG C^T[GA][CT]AG 2082 CTGCAG SfiI 426 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2304 GGCCCTCATGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII