(Circular) (Six-base) MAPSORT of: pFN21AB0454.seq Check: 255 from: 1 to: 7480 pFN21AB0454 7480 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5290 278 Size: 53 83 186 936 3754 2468 Fragments arranged by size: 3754 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4823 1937 Size: 2886 4594 Acc65I G'GTAC_C Cuts at: 2883 4801 2883 Size: 1918 5562 AclI AA'CG_TT Cuts at: 1563 5607 5980 1563 Size: 4044 373 3063 Fragments arranged by size: 4044 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3356 5543 6927 7195 1632 Size: 1724 2187 1384 268 1917 Fragments arranged by size: 2187 1917 1724 1384 268 AfeI AGC'GCT Cuts at: 2816 2816 Size: 7480 AflII C'TTAA_G Cuts at: 820 1017 3407 820 Size: 197 2390 4893 Fragments arranged by size: 4893 2390 197 AflIII A'CryG_T Cuts at: 1203 6379 7356 1203 Size: 5176 977 1327 Fragments arranged by size: 5176 1327 977 AgeI A'CCGG_T Cuts at: 6311 6311 Size: 7480 AhdI GACnn_n'nnGTC Cuts at: 4643 6209 4643 Size: 1566 5914 AleI CACnn'nnGTG Cuts at: 1166 3196 3626 1166 Size: 2030 430 5020 Fragments arranged by size: 5020 2030 430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4623 1184 Size: 3439 4041 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3800 3832 3800 Size: 32 7448 AlwNI CAG_nnn'CTG Cuts at: 3176 4462 4567 5223 6795 3176 Size: 1286 105 656 1572 3861 Fragments arranged by size: 3861 1572 1286 656 105 ApaI G_GGCC'C Cuts at: 2385 2598 3066 3271 2385 Size: 213 468 205 6594 Fragments arranged by size: 6594 468 213 205 ApaLI G'TGCA_C Cuts at: 3431 4361 5537 6693 3431 Size: 930 1176 1156 4218 Fragments arranged by size: 4218 1176 1156 930 ApoI r'AATT_y Cuts at: 1493 3864 4510 4789 5015 5167 7276 1493 Size: 2371 646 279 226 152 2109 1697 Fragments arranged by size: 2371 2109 1697 646 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 6034 160 Size: 872 5002 1606 Fragments arranged by size: 5002 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7480 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2323 2449 4573 4805 1870 Size: 73 72 308 126 2124 232 4545 Fragments arranged by size: 4545 2124 308 232 126 73 72 BamHI G'GATC_C Cuts at: 2955 3402 4810 5177 2955 Size: 447 1408 367 5258 Fragments arranged by size: 5258 1408 447 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2750 2854 2883 3142 4523 Size: 325 217 627 963 104 29 259 1381 Cuts at: 4523 4801 6256 618 Size: 278 1455 1842 Fragments arranged by size: 1842 1455 1381 963 627 325 278 259 217 104 29 BanII G_rGCy'C Cuts at: 1391 2250 2336 2385 2510 2598 2828 3066 3271 Size: 859 86 49 125 88 230 238 205 Cuts at: 3271 3519 4059 4209 4283 4388 4574 4799 1391 Size: 248 540 150 74 105 186 225 4072 Fragments arranged by size: 4072 859 540 248 238 230 225 205 186 150 125 105 88 86 74 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2981 3851 928 Size: 971 1082 870 4557 Fragments arranged by size: 4557 1082 971 870 BbvCI CC'TCA_GC Cuts at: 4014 4014 Size: 7480 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2304 2338 5671 5705 1903 Size: 34 367 34 3333 34 3678 Fragments arranged by size: 3678 3333 367 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5372 6582 5372 Size: 1210 6270 BclI T'GATC_A Cuts at: 1548 2910 1548 Size: 1362 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 3127 4178 Size: 107 122 71 631 605 766 689 1051 Cuts at: 4178 4293 4316 6091 136 Size: 115 23 1775 1525 Fragments arranged by size: 1775 1525 1051 766 689 631 605 122 115 107 71 23 BglII A'GATC_T Cuts at: 3375 7475 3375 Size: 4100 3380 BlpI GC'TnA_GC Cuts at: 4387 4568 4897 4387 Size: 181 329 6970 Fragments arranged by size: 6970 329 181 Bme1580I G_kGCm'C Cuts at: 1790 2385 2472 2598 2721 3066 3271 3435 4365 Size: 595 87 126 123 345 205 164 930 Cuts at: 4365 5541 6697 1790 Size: 1176 1156 2573 Fragments arranged by size: 2573 1176 1156 930 595 345 205 164 126 123 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7480 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3164 4028 5211 Size: 416 53 83 399 24 1747 864 1183 Cuts at: 5211 6169 442 Size: 958 1753 Fragments arranged by size: 1753 1747 1183 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2517 1056 Size: 1461 6019 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4174 4174 Size: 7480 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3081 3336 3521 6140 1239 Size: 1842 255 185 2619 2579 Fragments arranged by size: 2619 2579 1842 255 185 Bpu10I CC'TnA_GC Cuts at: 2330 4014 4277 2330 Size: 1684 263 5533 Fragments arranged by size: 5533 1684 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2524 3215 5605 6470 6768 2195 Size: 329 691 2390 865 298 2907 Fragments arranged by size: 2907 2390 865 691 329 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6143 882 Size: 617 4644 2219 Fragments arranged by size: 4644 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2851 5271 493 Size: 711 1647 2420 2702 Fragments arranged by size: 2702 2420 1647 711 BsaBI GATnn'nnATC Cuts at: 2345 7474 2345 Size: 5129 2351 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 4221 5287 5669 Size: 53 83 186 936 513 2175 1066 382 Cuts at: 5669 6304 275 Size: 635 1451 Fragments arranged by size: 2175 1451 1066 936 635 513 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2171 5913 6311 6585 6732 7143 2171 Size: 3742 398 274 147 411 2508 Fragments arranged by size: 3742 2508 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 2855 2885 4165 Size: 30 165 30 1134 30 288 30 1280 Cuts at: 4165 4195 4355 4385 1178 Size: 30 160 30 4273 Fragments arranged by size: 4273 1280 1134 288 165 160 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2515 2728 3097 1186 Size: 1329 213 369 5569 Fragments arranged by size: 5569 1329 369 213 BseYI C'CCAG_C Cuts at: 2811 2879 3714 4039 4131 4243 4283 4291 6683 Size: 68 835 325 92 112 40 8 2392 Cuts at: 6683 7179 2811 Size: 496 3112 Fragments arranged by size: 3112 2392 835 496 325 112 92 68 40 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3266 1163 Size: 573 1530 5377 Fragments arranged by size: 5377 1530 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2257 3048 4251 4930 5691 6719 Size: 275 186 256 791 1203 679 761 1028 Cuts at: 6719 1540 Size: 2301 Fragments arranged by size: 2301 1203 1028 791 761 679 275 256 186 BsiHKAI G_wGCw'C Cuts at: 3435 3713 4365 4388 5541 5626 6697 3435 Size: 278 652 23 1153 85 1071 4218 Fragments arranged by size: 4218 1153 1071 652 278 85 23 BsmI GAATG_Cn' Cuts at: 2149 2764 4612 4991 5084 2149 Size: 615 1848 379 93 4545 Fragments arranged by size: 4545 1848 615 379 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2391 2581 3593 3682 2391 Size: 190 1012 89 6189 Fragments arranged by size: 6189 1012 190 89 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2336 2385 2472 2510 2598 2721 Size: 399 460 86 49 87 38 88 123 Cuts at: 2721 2828 3066 3271 3435 3519 3713 4059 4209 Size: 107 238 205 164 84 194 346 150 Cuts at: 4209 4283 4365 4388 4574 4799 5541 5626 6697 Size: 74 82 23 186 225 742 85 1071 Cuts at: 6697 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 460 399 346 238 225 205 194 186 164 150 123 107 88 87 86 85 84 82 74 49 38 23 BspEI T'CCGG_A Cuts at: 2171 7143 2171 Size: 4972 2508 BspHI T'CATG_A Cuts at: 5369 7099 5369 Size: 1730 5750 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4678 4835 844 Size: 3834 157 3489 Fragments arranged by size: 3834 3489 157 BsrBI CCG'CTC Cuts at: 1394 1823 2020 2736 3400 4350 5367 1394 Size: 429 197 716 664 950 1017 3507 Fragments arranged by size: 3507 1017 950 716 664 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 5975 6149 65 Size: 1183 4727 174 1396 Fragments arranged by size: 4727 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2995 3020 4306 4367 6124 6311 1700 Size: 110 1185 25 1286 61 1757 187 2869 Fragments arranged by size: 2869 1757 1286 1185 187 110 61 25 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7480 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7480 BssSI C'ACGA_G Cuts at: 2057 3728 4166 4612 5540 6552 2057 Size: 1671 438 446 928 1012 2985 Fragments arranged by size: 2985 1671 1012 928 446 438 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7480 BstBI TT'CG_AA Cuts at: 6284 6284 Size: 7480 BstEII G'GTnAC_C Cuts at: 1184 4678 1184 Size: 3494 3986 BstXI CCAn_nnnn'nTGG Cuts at: 2063 3112 3139 3198 3499 4291 2063 Size: 1049 27 59 301 792 5252 Fragments arranged by size: 5252 1049 792 301 59 27 BstYI r'GATC_y Cuts at: 1972 2955 3375 3402 3555 3927 4810 5177 5562 Size: 983 420 27 153 372 883 367 385 Cuts at: 5562 5579 7031 7117 7129 7475 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 983 883 420 385 372 367 346 153 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2866 2937 3048 3340 3738 3882 4059 Size: 552 1801 71 111 292 398 144 177 Cuts at: 4059 4099 4176 513 Size: 40 77 3817 Fragments arranged by size: 3817 1801 552 398 292 177 144 111 77 71 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2727 487 Size: 683 56 1501 5240 Fragments arranged by size: 5240 1501 683 56 BtsI GCAGTG_nn' Cuts at: 2382 4996 5790 5810 2382 Size: 2614 794 20 4052 Fragments arranged by size: 4052 2614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7445 DraI TTT'AAA Cuts at: 4784 4784 Size: 7480 DraIII CAC_nnn'GTG Cuts at: 3328 3328 Size: 7480 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6487 809 Size: 507 5171 1802 Fragments arranged by size: 5171 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2254 2304 2430 Size: 54 1425 50 275 18 424 50 126 Cuts at: 2430 2439 3045 3200 3781 4146 4170 4248 4317 Size: 9 606 155 581 365 24 78 69 Cuts at: 4317 4927 5816 7445 8 Size: 610 889 1629 43 Fragments arranged by size: 1629 1425 889 610 606 581 424 365 275 155 126 78 69 54 50 50 43 24 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2254 3045 4248 4927 1537 Size: 275 442 791 1203 679 4090 Fragments arranged by size: 4090 1203 791 679 442 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2130 3989 5410 1353 Size: 755 22 1859 1421 3423 Fragments arranged by size: 3423 1859 1421 755 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3108 6067 6441 6587 387 Size: 1064 138 1519 2959 374 146 1280 Fragments arranged by size: 2959 1519 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4386 4386 Size: 7480 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3081 3336 3356 3521 5543 6140 6927 Size: 393 1449 255 20 165 2022 597 787 Cuts at: 6927 7195 1239 Size: 268 1524 Fragments arranged by size: 2022 1524 1449 787 597 393 268 255 165 20 EcoNI CCTnn'n_nnAGG Cuts at: 2684 2684 Size: 7480 EcoO109I rG'GnC_Cy Cuts at: 2319 2381 2382 3062 3063 4757 2319 Size: 62 1 680 1 1694 5042 Fragments arranged by size: 5042 1694 680 62 1 1 EcoRI G'AATT_C Cuts at: 4789 5167 4789 Size: 378 7102 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2102 2102 Size: 7480 FseI GG_CCGG'CC Cuts at: 3024 3024 Size: 7480 FspI TGC'GCA Cuts at: 5986 5986 Size: 7480 HaeII r_GCGC'y Cuts at: 1678 2818 6627 1678 Size: 1140 3809 2531 Fragments arranged by size: 3809 2531 1140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 2855 2887 3285 Size: 32 135 32 965 32 286 32 398 Cuts at: 3285 3317 3360 3392 3636 3668 4629 4661 6121 Size: 32 43 32 244 32 961 32 1460 Cuts at: 6121 6153 6195 6227 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1460 965 961 398 286 244 135 43 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4824 5070 669 Size: 988 281 2886 246 3079 Fragments arranged by size: 3079 2886 988 281 246 HindIII A'AGCT_T Cuts at: 748 3563 748 Size: 2815 4665 HpaI GTT'AAC Cuts at: 5070 5070 Size: 7480 KpnI G_GTAC'C Cuts at: 2887 4805 2887 Size: 1918 5562 MfeI C'AATT_G Cuts at: 5079 5079 Size: 7480 MluI A'CGCG_T Cuts at: 7356 7356 Size: 7480 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3335 4433 6594 6778 1305 Size: 2030 1098 2161 184 2007 Fragments arranged by size: 2161 2030 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4172 10 Size: 54 1425 343 2340 3318 Fragments arranged by size: 3318 2340 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3170 3196 3626 5438 5797 5956 518 Size: 648 2004 26 430 1812 359 159 2042 Fragments arranged by size: 2042 2004 1812 648 430 359 159 26 MspA1I CmG'CkG Cuts at: 1606 2520 2694 3139 3205 3392 3765 4247 4264 Size: 914 174 445 66 187 373 482 17 Cuts at: 4264 4327 4462 4896 5573 6721 6966 1606 Size: 63 135 434 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 677 482 445 434 373 245 187 174 135 66 63 17 NaeI GCC'GGC Cuts at: 3022 4308 4369 3022 Size: 1286 61 6133 Fragments arranged by size: 6133 1286 61 NcoI C'CATG_G Cuts at: 513 1065 3340 3738 4099 4176 513 Size: 552 2275 398 361 77 3817 Fragments arranged by size: 3817 2275 552 398 361 77 NdeI CA'TA_TG Cuts at: 387 387 Size: 7480 NgoMIV G'CCGG_C Cuts at: 3020 4306 4367 3020 Size: 1286 61 6133 Fragments arranged by size: 6133 1286 61 NheI G'CTAG_C Cuts at: 1052 2513 1052 Size: 1461 6019 NotI GC'GGCC_GC Cuts at: 4927 4927 Size: 7480 NspI r_CATG'y Cuts at: 1132 1229 3916 4838 6383 1132 Size: 97 2687 922 1545 2229 Fragments arranged by size: 2687 2229 1545 922 97 PasI CC'CwG_GG Cuts at: 1348 4375 1348 Size: 3027 4453 PciI A'CATG_T Cuts at: 6379 6379 Size: 7480 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2970 3328 1108 Size: 1269 593 358 5260 Fragments arranged by size: 5260 1269 593 358 PfoI T'CCnGG_A Cuts at: 2196 2819 3498 2196 Size: 623 679 6178 Fragments arranged by size: 6178 679 623 PmeI GTTT'AAAC Cuts at: 4784 4784 Size: 7480 PmlI CAC'GTG Cuts at: 2851 2851 Size: 7480 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4561 4593 5549 5581 7062 7094 4561 Size: 32 956 32 1481 32 4947 Fragments arranged by size: 4947 1481 956 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4757 4757 Size: 7480 PshAI GACnn'nnGTC Cuts at: 1613 2607 1613 Size: 994 6486 PsiI TTA'TAA Cuts at: 5050 5050 Size: 7480 PspOMI G'GGCC_C Cuts at: 2381 2594 3062 3267 2381 Size: 213 468 205 6594 Fragments arranged by size: 6594 468 213 205 PstI C_TGCA'G Cuts at: 830 3164 4091 4832 830 Size: 2334 927 741 3478 Fragments arranged by size: 3478 2334 927 741 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7480 PvuII CAG'CTG Cuts at: 2694 3392 3765 4462 2694 Size: 698 373 697 5712 Fragments arranged by size: 5712 698 697 373 SacI G_AGCT'C Cuts at: 4388 4388 Size: 7480 SalI G'TCGA_C Cuts at: 1936 4822 1936 Size: 2886 4594 SapI GCTCTTCn'nnn_ Cuts at: 2130 2130 Size: 7480 SbfI CC_TGCA'GG Cuts at: 4832 4832 Size: 7480 ScaI AGT'ACT Cuts at: 5728 7440 5728 Size: 1712 5768 SexAI A'CCwGG_T Cuts at: 3426 3426 Size: 7480 SfcI C'TryA_G Cuts at: 826 1045 3160 4087 4828 5963 6644 6835 826 Size: 219 2115 927 741 1135 681 191 1471 Fragments arranged by size: 2115 1471 1135 927 741 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2438 4316 2438 Size: 1878 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7480 SgrAI Cr'CCGG_yG Cuts at: 1700 2995 1700 Size: 1295 6185 SmaI CCC'GGG Cuts at: 1872 4575 4807 1872 Size: 2703 232 4545 Fragments arranged by size: 4545 2703 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 2503 3194 3407 5584 6485 Size: 197 926 267 293 691 213 2177 901 Cuts at: 6485 6747 820 Size: 262 1553 Fragments arranged by size: 2177 1553 926 901 691 293 267 262 213 197 SnaBI TAC'GTA Cuts at: 493 5271 493 Size: 4778 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7480 SphI G_CATG'C Cuts at: 1132 4838 1132 Size: 3706 3774 SspI AAT'ATT Cuts at: 5 52 5404 5 Size: 47 5352 2081 Fragments arranged by size: 5352 2081 47 StuI AGG'CCT Cuts at: 1517 2780 4107 4424 1517 Size: 1263 1327 317 4573 Fragments arranged by size: 4573 1327 1263 317 StyI C'CwwG_G Cuts at: 513 1065 2781 3015 3340 3510 3738 4099 4176 Size: 552 1716 234 325 170 228 361 77 Cuts at: 4176 4919 513 Size: 743 3074 Fragments arranged by size: 3074 1716 743 552 361 325 234 228 170 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4047 4112 4462 5507 5524 5677 5862 Size: 1255 2467 65 350 1045 17 153 185 Cuts at: 5862 6307 325 Size: 445 1498 Fragments arranged by size: 2467 1498 1255 1045 445 350 185 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4500 5726 7438 Size: 274 80 33 51 1443 2523 1226 1712 Cuts at: 7438 96 Size: 138 Fragments arranged by size: 2523 1712 1443 1226 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4165 4197 4355 4387 7440 7472 4165 Size: 32 158 32 3053 32 4173 Fragments arranged by size: 4173 3053 158 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7480 XbaI T'CTAG_A Cuts at: 4816 4816 Size: 7480 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7480 XmaI C'CCGG_G Cuts at: 1870 4573 4805 1870 Size: 2703 232 4545 Fragments arranged by size: 4545 2703 232 XmnI GAAnn'nnTTC Cuts at: 5609 5609 Size: 7480 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5288 276 Size: 53 83 186 936 3754 2468 Fragments arranged by size: 3754 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI XcmI