(Linear) (Six-base) MAPSORT of: KIBB0454.seq Check: 3134 from: 1 to: 2775 KIBB0454.orf With 182 enzymes: SgfI * May 6, 2010 17:03 .. Acc65I G'GTAC_C Cuts at: 0 878 2775 Size: 878 1897 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1351 2775 Size: 1351 1424 AfeI AGC'GCT Cuts at: 0 811 2775 Size: 811 1964 AflII C'TTAA_G Cuts at: 0 1402 2775 Size: 1402 1373 AhdI GACnn_n'nnGTC Cuts at: 0 2638 2775 Size: 2638 137 AleI CACnn'nnGTG Cuts at: 0 1191 1621 2775 Size: 1191 430 1154 Fragments arranged by size: 1191 1154 430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2618 2775 Size: 2618 157 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1795 1827 2775 Size: 1795 32 948 Fragments arranged by size: 1795 948 32 AlwNI CAG_nnn'CTG Cuts at: 0 1171 2457 2562 2775 Size: 1171 1286 105 213 Fragments arranged by size: 1286 1171 213 105 ApaI G_GGCC'C Cuts at: 0 380 593 1061 1266 2775 Size: 380 213 468 205 1509 Fragments arranged by size: 1509 468 380 213 205 ApaLI G'TGCA_C Cuts at: 0 1426 2356 2775 Size: 1426 930 419 Fragments arranged by size: 1426 930 419 ApoI r'AATT_y Cuts at: 0 1859 2505 2775 Size: 1859 646 270 Fragments arranged by size: 1859 646 270 AvaI C'yCGr_G Cuts at: 0 10 318 444 2568 2775 Size: 10 308 126 2124 207 Fragments arranged by size: 2124 308 207 126 10 BamHI G'GATC_C Cuts at: 0 950 1397 2775 Size: 950 447 1378 Fragments arranged by size: 1378 950 447 BanI G'GyrC_C Cuts at: 0 745 849 878 1137 2518 2775 Size: 745 104 29 259 1381 257 Fragments arranged by size: 1381 745 259 257 104 29 BanII G_rGCy'C Cuts at: 0 245 331 380 505 593 823 1061 1266 Size: 245 86 49 125 88 230 238 205 Cuts at: 1266 1514 2054 2204 2278 2383 2569 2775 Size: 248 540 150 74 105 186 206 Fragments arranged by size: 540 248 245 238 230 206 205 186 150 125 105 88 86 74 49 BbsI GAAGACnn'nnnn_ Cuts at: 0 976 1846 2775 Size: 976 870 929 Fragments arranged by size: 976 929 870 BbvCI CC'TCA_GC Cuts at: 0 2009 2775 Size: 2009 766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 299 333 2775 Size: 299 34 2442 Fragments arranged by size: 2442 299 34 BclI T'GATC_A Cuts at: 0 905 2775 Size: 905 1870 BglI GCCn_nnn'nGGC Cuts at: 0 433 1122 2173 2288 2311 2775 Size: 433 689 1051 115 23 464 Fragments arranged by size: 1051 689 464 433 115 23 BglII A'GATC_T Cuts at: 0 1370 2775 Size: 1370 1405 BlpI GC'TnA_GC Cuts at: 0 2382 2563 2775 Size: 2382 181 212 Fragments arranged by size: 2382 212 181 Bme1580I G_kGCm'C Cuts at: 0 380 467 593 716 1061 1266 1430 2360 Size: 380 87 126 123 345 205 164 930 Cuts at: 2360 2775 Size: 415 Fragments arranged by size: 930 415 380 345 205 164 126 123 87 BmrI ACTGGGnnnn_n' Cuts at: 0 1159 2023 2775 Size: 1159 864 752 Fragments arranged by size: 1159 864 752 BmtI G_CTAG'C Cuts at: 0 512 2775 Size: 512 2263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2169 2775 Size: 2169 606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1331 1516 2775 Size: 1076 255 185 1259 Fragments arranged by size: 1259 1076 255 185 Bpu10I CC'TnA_GC Cuts at: 0 325 2009 2272 2775 Size: 325 1684 263 503 Fragments arranged by size: 1684 503 325 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 519 1210 2775 Size: 190 329 691 1565 Fragments arranged by size: 1565 691 329 190 BsaAI yAC'GTr Cuts at: 0 846 2775 Size: 846 1929 BsaBI GATnn'nnATC Cuts at: 0 340 2775 Size: 340 2435 BsaHI Gr'CG_yC Cuts at: 0 41 2216 2775 Size: 41 2175 559 Fragments arranged by size: 2175 559 41 BsaWI w'CCGG_w Cuts at: 0 166 2775 Size: 166 2609 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 532 562 850 880 2160 2190 2350 2380 Size: 532 30 288 30 1280 30 160 30 Cuts at: 2380 2775 Size: 395 Fragments arranged by size: 1280 532 395 288 160 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 510 723 1092 2775 Size: 510 213 369 1683 Fragments arranged by size: 1683 510 369 213 BseYI C'CCAG_C Cuts at: 0 806 874 1709 2034 2126 2238 2278 2286 Size: 806 68 835 325 92 112 40 8 Cuts at: 2286 2775 Size: 489 Fragments arranged by size: 835 806 489 325 112 92 68 40 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1261 2775 Size: 1261 1514 BsiEI CG_ry'CG Cuts at: 0 252 1043 2246 2775 Size: 252 791 1203 529 Fragments arranged by size: 1203 791 529 252 BsiHKAI G_wGCw'C Cuts at: 0 1430 1708 2360 2383 2775 Size: 1430 278 652 23 392 Fragments arranged by size: 1430 652 392 278 23 BsmI GAATG_Cn' Cuts at: 0 144 759 2607 2775 Size: 144 615 1848 168 Fragments arranged by size: 1848 615 168 144 BsmBI CGTCTCn'nnnn_ Cuts at: 0 386 576 1588 1677 2775 Size: 386 190 1012 89 1098 Fragments arranged by size: 1098 1012 386 190 89 Bsp1286I G_dGCh'C Cuts at: 0 245 331 380 467 505 593 716 823 Size: 245 86 49 87 38 88 123 107 Cuts at: 823 1061 1266 1430 1514 1708 2054 2204 2278 Size: 238 205 164 84 194 346 150 74 Cuts at: 2278 2360 2383 2569 2775 Size: 82 23 186 206 Fragments arranged by size: 346 245 238 206 205 194 186 164 150 123 107 88 87 86 84 82 74 49 38 23 BspEI T'CCGG_A Cuts at: 0 166 2775 Size: 166 2609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2673 2775 Size: 2673 102 BsrBI CCG'CTC Cuts at: 0 15 731 1395 2345 2775 Size: 15 716 664 950 430 Fragments arranged by size: 950 716 664 430 15 BsrFI r'CCGG_y Cuts at: 0 990 1015 2301 2362 2775 Size: 990 25 1286 61 413 Fragments arranged by size: 1286 990 413 61 25 BssSI C'ACGA_G Cuts at: 0 52 1723 2161 2607 2775 Size: 52 1671 438 446 168 Fragments arranged by size: 1671 446 438 168 52 BstEII G'GTnAC_C Cuts at: 0 2673 2775 Size: 2673 102 BstXI CCAn_nnnn'nTGG Cuts at: 0 58 1107 1134 1193 1494 2286 2775 Size: 58 1049 27 59 301 792 489 Fragments arranged by size: 1049 792 489 301 59 58 27 BstYI r'GATC_y Cuts at: 0 950 1370 1397 1550 1922 2775 Size: 950 420 27 153 372 853 Fragments arranged by size: 950 853 420 372 153 27 BtgI C'CryG_G Cuts at: 0 861 932 1043 1335 1733 1877 2054 2094 Size: 861 71 111 292 398 144 177 40 Cuts at: 2094 2171 2775 Size: 77 604 Fragments arranged by size: 861 604 398 292 177 144 111 77 71 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 722 2775 Size: 722 2053 BtsI GCAGTG_nn' Cuts at: 0 377 2775 Size: 377 2398 DraIII CAC_nnn'GTG Cuts at: 0 1323 2775 Size: 1323 1452 EaeI y'GGCC_r Cuts at: 0 249 299 425 434 1040 1195 1776 2141 Size: 249 50 126 9 606 155 581 365 Cuts at: 2141 2165 2243 2312 2775 Size: 24 78 69 463 Fragments arranged by size: 606 581 463 365 249 155 126 78 69 50 24 9 EagI C'GGCC_G Cuts at: 0 249 1040 2243 2775 Size: 249 791 1203 532 Fragments arranged by size: 1203 791 532 249 EarI CTCTTCn'nnn_ Cuts at: 0 103 125 1984 2775 Size: 103 22 1859 791 Fragments arranged by size: 1859 791 103 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1103 2775 Size: 1103 1672 EcoICRI GAG'CTC Cuts at: 0 2381 2775 Size: 2381 394 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1331 1351 1516 2775 Size: 1076 255 20 165 1259 Fragments arranged by size: 1259 1076 255 165 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 679 2775 Size: 679 2096 EcoO109I rG'GnC_Cy Cuts at: 0 314 376 377 1057 1058 2752 2775 Size: 314 62 1 680 1 1694 23 Fragments arranged by size: 1694 680 314 62 23 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 97 2775 Size: 97 2678 FseI GG_CCGG'CC Cuts at: 0 1019 2775 Size: 1019 1756 HaeII r_GCGC'y Cuts at: 0 813 2775 Size: 813 1962 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 532 564 850 882 1280 1312 1355 1387 Size: 532 32 286 32 398 32 43 32 Cuts at: 1387 1631 1663 2624 2656 2775 Size: 244 32 961 32 119 Fragments arranged by size: 961 532 398 286 244 119 43 32 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1558 2775 Size: 1558 1217 KpnI G_GTAC'C Cuts at: 0 882 2775 Size: 882 1893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1330 2428 2775 Size: 1330 1098 347 Fragments arranged by size: 1330 1098 347 MscI TGG'CCA Cuts at: 0 2167 2775 Size: 2167 608 MslI CAynn'nnrTG Cuts at: 0 1165 1191 1621 2775 Size: 1165 26 430 1154 Fragments arranged by size: 1165 1154 430 26 MspA1I CmG'CkG Cuts at: 0 515 689 1134 1200 1387 1760 2242 2259 Size: 515 174 445 66 187 373 482 17 Cuts at: 2259 2322 2457 2775 Size: 63 135 318 Fragments arranged by size: 515 482 445 373 318 187 174 135 66 63 17 NaeI GCC'GGC Cuts at: 0 1017 2303 2364 2775 Size: 1017 1286 61 411 Fragments arranged by size: 1286 1017 411 61 NcoI C'CATG_G Cuts at: 0 1335 1733 2094 2171 2775 Size: 1335 398 361 77 604 Fragments arranged by size: 1335 604 398 361 77 NgoMIV G'CCGG_C Cuts at: 0 1015 2301 2362 2775 Size: 1015 1286 61 413 Fragments arranged by size: 1286 1015 413 61 NheI G'CTAG_C Cuts at: 0 508 2775 Size: 508 2267 NspI r_CATG'y Cuts at: 0 1911 2775 Size: 1911 864 PasI CC'CwG_GG Cuts at: 0 2370 2775 Size: 2370 405 PflMI CCAn_nnn'nTGG Cuts at: 0 372 965 1323 2775 Size: 372 593 358 1452 Fragments arranged by size: 1452 593 372 358 PfoI T'CCnGG_A Cuts at: 0 191 814 1493 2775 Size: 191 623 679 1282 Fragments arranged by size: 1282 679 623 191 PmlI CAC'GTG Cuts at: 0 846 2775 Size: 846 1929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2556 2588 2775 Size: 2556 32 187 Fragments arranged by size: 2556 187 32 PpuMI rG'GwC_Cy Cuts at: 0 2752 2775 Size: 2752 23 PshAI GACnn'nnGTC Cuts at: 0 602 2775 Size: 602 2173 PspOMI G'GGCC_C Cuts at: 0 376 589 1057 1262 2775 Size: 376 213 468 205 1513 Fragments arranged by size: 1513 468 376 213 205 PstI C_TGCA'G Cuts at: 0 1159 2086 2775 Size: 1159 927 689 Fragments arranged by size: 1159 927 689 PvuII CAG'CTG Cuts at: 0 689 1387 1760 2457 2775 Size: 689 698 373 697 318 Fragments arranged by size: 698 697 689 373 318 SacI G_AGCT'C Cuts at: 0 2383 2775 Size: 2383 392 SapI GCTCTTCn'nnn_ Cuts at: 0 125 2775 Size: 125 2650 SexAI A'CCwGG_T Cuts at: 0 1421 2775 Size: 1421 1354 SfcI C'TryA_G Cuts at: 0 1155 2082 2775 Size: 1155 927 693 Fragments arranged by size: 1155 927 693 SfiI GGCCn_nnn'nGGCC Cuts at: 0 433 2311 2775 Size: 433 1878 464 Fragments arranged by size: 1878 464 433 SgrAI Cr'CCGG_yG Cuts at: 0 990 2775 Size: 990 1785 SmaI CCC'GGG Cuts at: 0 2570 2775 Size: 2570 205 SmlI C'TyrA_G Cuts at: 0 205 498 1189 1402 2775 Size: 205 293 691 213 1373 Fragments arranged by size: 1373 691 293 213 205 StuI AGG'CCT Cuts at: 0 775 2102 2419 2775 Size: 775 1327 317 356 Fragments arranged by size: 1327 775 356 317 StyI C'CwwG_G Cuts at: 0 776 1010 1335 1505 1733 2094 2171 2775 Size: 776 234 325 170 228 361 77 604 Fragments arranged by size: 776 604 361 325 234 228 170 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2042 2107 2457 2775 Size: 2042 65 350 318 Fragments arranged by size: 2042 350 318 65 TatI w'GTAC_w Cuts at: 0 2495 2775 Size: 2495 280 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2160 2192 2350 2382 2775 Size: 2160 32 158 32 393 Fragments arranged by size: 2160 393 158 32 32 XmaI C'CCGG_G Cuts at: 0 2568 2775 Size: 2568 207 Enzymes that do cut: Acc65I AcuI AfeI AflII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SapI SexAI SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflIII AgeI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BmgBI BsaI BsiWI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FspI FspAI HincII HpaI KasI MfeI MluI NarI NdeI NotI NruI NsiI PacI PciI PmeI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI