(Circular) (Six-base) MAPSORT of: pFN21AE9696.seq Check: 6586 from: 1 to: 10693 pFN21AE9696 10693 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2662 3188 6442 2662 Size: 526 3254 6913 Fragments arranged by size: 6913 3254 526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8503 278 Size: 53 83 186 936 6967 2468 Fragments arranged by size: 6967 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6136 6926 8036 1937 Size: 4199 790 1110 4594 Fragments arranged by size: 4594 4199 1110 790 Acc65I G'GTAC_C Cuts at: 6891 8014 6891 Size: 1123 9570 AclI AA'CG_TT Cuts at: 1563 8820 9193 1563 Size: 7257 373 3063 Fragments arranged by size: 7257 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2482 3527 3680 5228 5712 6147 7347 7664 Size: 850 1045 153 1548 484 435 1200 317 Cuts at: 7664 7780 8756 10140 10408 1632 Size: 116 976 1384 268 1917 Fragments arranged by size: 1917 1548 1384 1200 1045 976 850 484 435 317 268 153 116 AfeI AGC'GCT Cuts at: 6572 6572 Size: 10693 AflII C'TTAA_G Cuts at: 820 1017 5066 820 Size: 197 4049 6447 Fragments arranged by size: 6447 4049 197 AflIII A'CryG_T Cuts at: 1203 3408 4203 9592 10569 1203 Size: 2205 795 5389 977 1327 Fragments arranged by size: 5389 2205 1327 977 795 AgeI A'CCGG_T Cuts at: 5538 9524 5538 Size: 3986 6707 AhdI GACnn_n'nnGTC Cuts at: 6070 9422 6070 Size: 3352 7341 AleI CACnn'nnGTG Cuts at: 1166 5579 5816 1166 Size: 4413 237 6043 Fragments arranged by size: 6043 4413 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10693 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 4385 4417 4652 4684 2438 Size: 32 1915 32 235 32 8447 Fragments arranged by size: 8447 1915 235 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2021 2624 2627 2660 2684 2954 4187 6310 6642 Size: 603 3 33 24 270 1233 2123 332 Cuts at: 6642 6782 7402 8436 10008 2021 Size: 140 620 1034 1572 2706 Fragments arranged by size: 2706 2123 1572 1233 1034 620 603 332 270 140 33 24 3 ApaI G_GGCC'C Cuts at: 4249 5374 7819 4249 Size: 1125 2445 7123 Fragments arranged by size: 7123 2445 1125 ApaLI G'TGCA_C Cuts at: 8750 9906 8750 Size: 1156 9537 ApoI r'AATT_y Cuts at: 1493 2231 4421 4481 4999 5014 5681 8002 8228 Size: 738 2190 60 518 15 667 2321 226 Cuts at: 8228 8380 10489 1493 Size: 152 2109 1697 Fragments arranged by size: 2321 2190 2109 1697 738 667 518 226 152 60 15 AseI AT'TA_AT Cuts at: 160 1032 9247 160 Size: 872 8215 1606 Fragments arranged by size: 8215 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10693 AvaI C'yCGr_G Cuts at: 1870 1943 2829 2926 5400 6195 6429 7056 7189 Size: 73 886 97 2474 795 234 627 133 Cuts at: 7189 7962 8018 1870 Size: 773 56 4545 Fragments arranged by size: 4545 2474 886 795 773 627 234 133 97 73 56 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6875 6908 6875 Size: 33 10660 BamHI G'GATC_C Cuts at: 8023 8390 8023 Size: 367 10326 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2872 4193 4414 5327 6853 Size: 325 217 627 1085 1321 221 913 1526 Cuts at: 6853 6862 6891 7832 8014 9469 618 Size: 9 29 941 182 1455 1842 Fragments arranged by size: 1842 1526 1455 1321 1085 941 913 627 325 221 217 182 29 9 BanII G_rGCy'C Cuts at: 1391 2828 3232 3396 3762 4249 4758 4938 5374 Size: 1437 404 164 366 487 509 180 436 Cuts at: 5374 5503 5636 7819 7962 8012 1391 Size: 129 133 2183 143 50 4072 Fragments arranged by size: 4072 2183 1437 509 487 436 404 366 180 164 143 133 129 50 BbeI G_GCGC'C Cuts at: 7836 7836 Size: 10693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2351 4068 4303 5886 5991 6063 6114 Size: 971 452 1717 235 1583 105 72 51 Cuts at: 6114 7225 928 Size: 1111 4396 Fragments arranged by size: 4396 1717 1583 1111 971 452 235 105 72 51 BbvCI CC'TCA_GC Cuts at: 2057 2955 5957 7703 2057 Size: 898 3002 1746 5047 Fragments arranged by size: 5047 3002 1746 898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3477 3511 8884 8918 1903 Size: 34 1540 34 5373 34 3678 Fragments arranged by size: 5373 3678 1540 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8585 9795 8585 Size: 1210 9483 BclI T'GATC_A Cuts at: 1548 3352 4584 4594 1548 Size: 1804 1232 10 7647 Fragments arranged by size: 7647 1804 1232 10 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 9304 136 Size: 107 122 71 631 605 337 7295 1525 Fragments arranged by size: 7295 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7206 10688 7206 Size: 3482 7211 BlpI GC'TnA_GC Cuts at: 2667 4955 7607 8110 2667 Size: 2288 2652 503 5250 Fragments arranged by size: 5250 2652 2288 503 Bme1580I G_kGCm'C Cuts at: 1790 4249 4256 4369 4417 5155 5332 5374 7727 Size: 2459 7 113 48 738 177 42 2353 Cuts at: 7727 7819 8754 9910 1790 Size: 92 935 1156 2573 Fragments arranged by size: 2573 2459 2353 1156 935 738 177 113 92 48 42 7 BmgBI CAC'GTC Cuts at: 1318 5818 7627 1318 Size: 4500 1809 4384 Fragments arranged by size: 4500 4384 1809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4119 4399 5124 Size: 416 53 83 399 24 2702 280 725 Cuts at: 5124 5953 8424 9382 442 Size: 829 2471 958 1753 Fragments arranged by size: 2702 2471 1753 958 829 725 416 399 280 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10693 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3270 3270 Size: 10693 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2456 3216 4054 4209 4769 4835 5171 Size: 819 398 760 838 155 560 66 336 Cuts at: 5171 6562 6648 6842 6888 7370 7412 7616 7875 Size: 1391 86 194 46 482 42 204 259 Cuts at: 7875 9353 1239 Size: 1478 2579 Fragments arranged by size: 2579 1478 1391 838 819 760 560 482 398 336 259 204 194 155 86 66 46 42 Bpu10I CC'TnA_GC Cuts at: 2057 2955 3216 5957 6051 7703 2057 Size: 898 261 2741 94 1652 5047 Fragments arranged by size: 5047 2741 1652 898 261 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4640 4880 5288 6725 8818 9683 9981 4640 Size: 240 408 1437 2093 865 298 5352 Fragments arranged by size: 5352 2093 1437 865 408 298 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9356 882 Size: 617 7857 2219 Fragments arranged by size: 7857 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 8484 493 Size: 711 2774 4506 2702 Fragments arranged by size: 4506 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 2564 3588 10687 2564 Size: 1024 7099 2570 Fragments arranged by size: 7099 2570 1024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6929 7833 8500 8882 Size: 53 83 186 936 5396 904 667 382 Cuts at: 8882 9517 275 Size: 635 1451 Fragments arranged by size: 5396 1451 936 904 667 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2294 2714 5538 5787 6156 9126 9524 9798 9945 Size: 420 2824 249 369 2970 398 274 147 Cuts at: 9945 10356 2294 Size: 411 2631 Fragments arranged by size: 2970 2824 2631 420 411 398 369 274 249 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 3383 3413 3764 Size: 30 165 30 1035 30 915 30 351 Cuts at: 3764 3794 3836 3866 4385 4415 6652 6682 6941 Size: 30 42 30 519 30 2237 30 259 Cuts at: 6941 6971 1178 Size: 30 4900 Fragments arranged by size: 4900 2237 1035 915 519 351 259 165 42 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2477 3110 3113 3329 3356 3359 3409 Size: 1054 237 633 3 216 27 3 50 Cuts at: 3409 3860 4766 4832 5747 5936 6110 6113 6565 Size: 451 906 66 915 189 174 3 452 Cuts at: 6565 6577 6962 7427 7613 1186 Size: 12 385 465 186 4266 Fragments arranged by size: 4266 1054 915 906 633 465 452 451 385 237 216 189 186 174 66 50 27 12 3 3 3 BseYI C'CCAG_C Cuts at: 4071 4620 4870 5405 5629 6220 6270 6554 6791 Size: 549 250 535 224 591 50 284 237 Cuts at: 6791 6796 7241 9896 10392 4071 Size: 5 445 2655 496 4372 Fragments arranged by size: 4372 2655 591 549 535 496 445 284 250 237 224 50 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3539 4184 5840 6518 6628 7559 1163 Size: 573 1803 645 1656 678 110 931 4297 Fragments arranged by size: 4297 1803 1656 931 678 645 573 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5353 8143 8904 9932 1540 Size: 275 186 3352 2790 761 1028 2301 Fragments arranged by size: 3352 2790 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3396 4758 4938 5232 6513 8754 8839 9910 3396 Size: 1362 180 294 1281 2241 85 1071 4179 Fragments arranged by size: 4179 2241 1362 1281 1071 294 180 85 BsmI GAATG_Cn' Cuts at: 8204 8297 8204 Size: 93 10600 BsmBI CGTCTCn'nnnn_ Cuts at: 3110 6609 3110 Size: 3499 7194 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2828 3232 3396 3762 4249 4256 4369 Size: 399 1038 404 164 366 487 7 113 Cuts at: 4369 4417 4758 4938 5155 5232 5332 5374 5503 Size: 48 341 180 217 77 100 42 129 Cuts at: 5503 5636 6513 7727 7819 7962 8012 8754 8839 Size: 133 877 1214 92 143 50 742 85 Cuts at: 8839 9910 1391 Size: 1071 2174 Fragments arranged by size: 2174 1214 1071 1038 877 742 487 404 399 366 341 217 180 164 143 133 129 113 100 92 85 77 50 48 42 7 BspEI T'CCGG_A Cuts at: 2714 6156 10356 2714 Size: 3442 4200 3051 Fragments arranged by size: 4200 3442 3051 BspHI T'CATG_A Cuts at: 2508 5055 8582 10312 2508 Size: 2547 3527 1730 2889 Fragments arranged by size: 3527 2889 2547 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2662 3188 6442 6664 8048 844 Size: 1818 526 3254 222 1384 3489 Fragments arranged by size: 3489 3254 1818 1384 526 222 BsrBI CCG'CTC Cuts at: 1394 1823 4396 6817 8580 1394 Size: 429 2573 2421 1763 3507 Fragments arranged by size: 3507 2573 2421 1763 429 BsrDI GCAATG_nn' Cuts at: 65 1248 9188 9362 65 Size: 1183 7940 174 1396 Fragments arranged by size: 7940 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3000 3042 5538 7456 7558 7707 7744 Size: 110 1190 42 2496 1918 102 149 37 Cuts at: 7744 9337 9524 1700 Size: 1593 187 2869 Fragments arranged by size: 2869 2496 1918 1593 1190 187 149 110 102 42 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10693 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10693 BssSI C'ACGA_G Cuts at: 3186 5019 8753 9765 3186 Size: 1833 3734 1012 4114 Fragments arranged by size: 4114 3734 1833 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4867 794 Size: 4073 6620 BstBI TT'CG_AA Cuts at: 9497 9497 Size: 10693 BstEII G'GTnAC_C Cuts at: 1184 7031 1184 Size: 5847 4846 BstXI CCAn_nnnn'nTGG Cuts at: 3155 3155 Size: 10693 BstYI r'GATC_y Cuts at: 1972 2541 3525 5062 7206 8023 8390 8775 8792 Size: 569 984 1537 2144 817 367 385 17 Cuts at: 8792 10244 10330 10342 10688 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2144 1977 1537 1452 984 817 569 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2995 5333 6323 6618 2995 Size: 2338 990 295 7070 Fragments arranged by size: 7070 2338 990 295 BtgI C'CryG_G Cuts at: 513 1065 4169 5100 6895 7067 7255 7470 513 Size: 552 3104 931 1795 172 188 215 3736 Fragments arranged by size: 3736 3104 1795 931 552 215 188 172 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5755 7651 487 Size: 683 56 4529 1896 3529 Fragments arranged by size: 4529 3529 1896 683 56 BtsI GCAGTG_nn' Cuts at: 2287 2986 3090 3567 3673 5430 5749 6176 6185 Size: 699 104 477 106 1757 319 427 9 Cuts at: 6185 8209 9003 9023 2287 Size: 2024 794 20 3957 Fragments arranged by size: 3957 2024 1757 794 699 477 427 319 106 104 20 9 ClaI AT'CG_AT Cuts at: 1554 5310 5349 1554 Size: 3756 39 6898 Fragments arranged by size: 6898 3756 39 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3416 3451 529 Size: 35 2852 35 7771 Fragments arranged by size: 7771 2852 35 35 DraI TTT'AAA Cuts at: 7321 7997 7321 Size: 676 10017 DraIII CAC_nnn'GTG Cuts at: 3913 5024 5227 6306 7158 3913 Size: 1111 203 1079 852 7448 Fragments arranged by size: 7448 1111 1079 852 203 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 9700 809 Size: 507 4238 4146 1802 Fragments arranged by size: 4238 4146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 3044 3087 Size: 54 1425 50 275 18 864 350 43 Cuts at: 3087 5427 5512 5583 7509 7560 7713 7821 8140 Size: 2340 85 71 1926 51 153 108 319 Cuts at: 8140 9029 10658 8 Size: 889 1629 43 Fragments arranged by size: 2340 1926 1629 1425 889 864 350 319 275 153 108 85 71 54 51 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8140 1537 Size: 275 6328 4090 Fragments arranged by size: 6328 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2767 3306 3621 3794 4529 4763 5559 Size: 791 623 539 315 173 735 234 796 Cuts at: 5559 5688 8623 1353 Size: 129 2935 3423 Fragments arranged by size: 3423 2935 796 791 735 623 539 315 234 173 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2371 2830 7637 9280 9654 9800 Size: 1064 138 782 459 4807 1643 374 146 Cuts at: 9800 387 Size: 1280 Fragments arranged by size: 4807 1643 1280 1064 782 459 374 146 138 EcoICRI GAG'CTC Cuts at: 3394 4756 4936 3394 Size: 1362 180 9151 Fragments arranged by size: 9151 1362 180 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2456 2482 3216 3527 3680 4054 Size: 393 426 398 26 734 311 153 374 Cuts at: 4054 4209 4769 4835 5171 5228 5712 6147 6562 Size: 155 560 66 336 57 484 435 415 Cuts at: 6562 6648 6842 6888 7347 7370 7412 7616 7664 Size: 86 194 46 459 23 42 204 48 Cuts at: 7664 7780 7875 8756 9353 10140 10408 1239 Size: 116 95 881 597 787 268 1524 Fragments arranged by size: 1524 881 787 734 597 560 484 459 435 426 415 398 393 374 336 311 268 204 194 155 153 116 95 86 66 57 48 46 42 26 23 EcoNI CCTnn'n_nnAGG Cuts at: 3618 5336 7394 7448 7532 7577 3618 Size: 1718 2058 54 84 45 6734 Fragments arranged by size: 6734 2058 1718 84 54 45 EcoO109I rG'GnC_Cy Cuts at: 2162 2431 2514 2939 3067 4349 5370 5410 6319 Size: 269 83 425 128 1282 1021 40 909 Cuts at: 6319 6424 7052 7760 7815 7816 7860 2162 Size: 105 628 708 55 1 44 4995 Fragments arranged by size: 4995 1282 1021 909 708 628 425 269 128 105 83 55 44 40 1 EcoRI G'AATT_C Cuts at: 4481 5014 8002 8380 4481 Size: 533 2988 378 6794 Fragments arranged by size: 6794 2988 533 378 EcoRV GAT'ATC Cuts at: 2752 2752 Size: 10693 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7360 7360 Size: 10693 FseI GG_CCGG'CC Cuts at: 3004 3046 3004 Size: 42 10651 FspI TGC'GCA Cuts at: 9199 9199 Size: 10693 HaeII r_GCGC'y Cuts at: 1678 6561 6574 7403 7803 7836 9840 1678 Size: 4883 13 829 400 33 2004 2531 Fragments arranged by size: 4883 2531 2004 829 400 33 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2660 2692 3764 3796 5217 Size: 32 135 32 1088 32 1072 32 1421 Cuts at: 5217 5249 5895 5927 6056 6088 7055 7087 9334 Size: 32 646 32 129 32 967 32 2247 Cuts at: 9334 9366 9408 9440 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2247 1421 1088 1072 967 646 135 129 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6034 6459 6927 8037 8283 669 Size: 988 281 4096 425 468 1110 246 3079 Fragments arranged by size: 4096 3079 1110 988 468 425 281 246 HindIII A'AGCT_T Cuts at: 748 2852 4521 748 Size: 2104 1669 6920 Fragments arranged by size: 6920 2104 1669 HpaI GTT'AAC Cuts at: 6034 8283 6034 Size: 2249 8444 KasI G'GCGC_C Cuts at: 7832 7832 Size: 10693 KpnI G_GTAC'C Cuts at: 6895 8018 6895 Size: 1123 9570 MfeI C'AATT_G Cuts at: 8292 8292 Size: 10693 MluI A'CGCG_T Cuts at: 3408 10569 3408 Size: 7161 3532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2285 4194 5763 6939 9807 9991 1305 Size: 980 1909 1569 1176 2868 184 2007 Fragments arranged by size: 2868 2007 1909 1569 1176 980 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5429 5514 5585 7715 10 Size: 54 1425 343 3597 85 71 2130 2988 Fragments arranged by size: 3597 2988 2130 1425 343 85 71 54 MslI CAynn'nnrTG Cuts at: 518 1166 5579 5816 5967 6215 6381 6516 7260 Size: 648 4413 237 151 248 166 135 744 Cuts at: 7260 8651 9010 9169 518 Size: 1391 359 159 2042 Fragments arranged by size: 4413 2042 1391 744 648 359 248 237 166 159 151 135 MspA1I CmG'CkG Cuts at: 1606 2056 2624 2642 2660 2681 3700 3739 4620 Size: 450 568 18 18 21 1019 39 881 Cuts at: 4620 6241 7420 7594 8109 8786 9934 10179 1606 Size: 1621 1179 174 515 677 1148 245 2120 Fragments arranged by size: 2120 1621 1179 1148 1019 881 677 568 515 450 245 174 39 21 18 18 NaeI GCC'GGC Cuts at: 3002 3044 7458 7560 7709 3002 Size: 42 4414 102 149 5986 Fragments arranged by size: 5986 4414 149 102 42 NarI GG'CG_CC Cuts at: 7833 7833 Size: 10693 NcoI C'CATG_G Cuts at: 513 1065 7255 7470 513 Size: 552 6190 215 3736 Fragments arranged by size: 6190 3736 552 215 NdeI CA'TA_TG Cuts at: 387 3964 387 Size: 3577 7116 NgoMIV G'CCGG_C Cuts at: 3000 3042 7456 7558 7707 3000 Size: 42 4414 102 149 5986 Fragments arranged by size: 5986 4414 149 102 42 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10693 NotI GC'GGCC_GC Cuts at: 8140 8140 Size: 10693 NspI r_CATG'y Cuts at: 1132 1229 2453 3498 4207 4885 4921 8051 9596 Size: 97 1224 1045 709 678 36 3130 1545 Cuts at: 9596 1132 Size: 2229 Fragments arranged by size: 3130 2229 1545 1224 1045 709 678 97 36 PasI CC'CwG_GG Cuts at: 1348 4249 1348 Size: 2901 7792 PciI A'CATG_T Cuts at: 4203 9592 4203 Size: 5389 5304 PflMI CCAn_nnn'nTGG Cuts at: 1108 3913 4620 6306 6809 1108 Size: 2805 707 1686 503 4992 Fragments arranged by size: 4992 2805 1686 707 503 PfoI T'CCnGG_A Cuts at: 2074 4812 7389 2074 Size: 2738 2577 5378 Fragments arranged by size: 5378 2738 2577 PmeI GTTT'AAAC Cuts at: 7997 7997 Size: 10693 PmlI CAC'GTG Cuts at: 3978 3978 Size: 10693 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 3623 3655 4385 4417 8762 8794 10275 Size: 32 1153 32 730 32 4345 32 1481 Cuts at: 10275 10307 2438 Size: 32 2824 Fragments arranged by size: 4345 2824 1481 1153 730 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2162 2939 3067 4349 6319 6424 7760 7860 2162 Size: 777 128 1282 1970 105 1336 100 4995 Fragments arranged by size: 4995 1970 1336 1282 777 128 105 100 PshAI GACnn'nnGTC Cuts at: 1613 2937 1613 Size: 1324 9369 PsiI TTA'TAA Cuts at: 8263 8263 Size: 10693 PspOMI G'GGCC_C Cuts at: 4245 5370 7815 4245 Size: 1125 2445 7123 Fragments arranged by size: 7123 2445 1125 PspXI vC'TCGA_Gb Cuts at: 2926 2926 Size: 10693 PstI C_TGCA'G Cuts at: 830 3085 7858 8045 830 Size: 2255 4773 187 3478 Fragments arranged by size: 4773 3478 2255 187 PvuI CG_AT'CG Cuts at: 2001 5353 2001 Size: 3352 7341 PvuII CAG'CTG Cuts at: 2056 2624 2660 2681 3700 7420 7594 2056 Size: 568 36 21 1019 3720 174 5155 Fragments arranged by size: 5155 3720 1019 568 174 36 21 RsrII CG'GwC_CG Cuts at: 2297 2297 Size: 10693 SacI G_AGCT'C Cuts at: 3396 4758 4938 3396 Size: 1362 180 9151 Fragments arranged by size: 9151 1362 180 SalI G'TCGA_C Cuts at: 1936 6925 8035 1936 Size: 4989 1110 4594 Fragments arranged by size: 4989 4594 1110 SanDI GG'GwC_CC Cuts at: 6424 7760 6424 Size: 1336 9357 SapI GCTCTTCn'nnn_ Cuts at: 2767 3794 4763 5559 5688 2767 Size: 1027 969 796 129 7772 Fragments arranged by size: 7772 1027 969 796 129 SbfI CC_TGCA'GG Cuts at: 7858 8045 7858 Size: 187 10506 ScaI AGT'ACT Cuts at: 3365 3854 6080 8941 10653 3365 Size: 489 2226 2861 1712 3405 Fragments arranged by size: 3405 2861 2226 1712 489 SexAI A'CCwGG_T Cuts at: 5034 5610 5034 Size: 576 10117 SfcI C'TryA_G Cuts at: 826 1045 2661 3081 3555 4106 7854 8041 9176 Size: 219 1616 420 474 551 3748 187 1135 Cuts at: 9176 9857 10048 826 Size: 681 191 1471 Fragments arranged by size: 3748 1616 1471 1135 681 551 474 420 219 191 187 SfoI GGC'GCC Cuts at: 7834 7834 Size: 10693 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10693 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10693 SmaI CCC'GGG Cuts at: 1872 8020 1872 Size: 6148 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2926 4655 4895 5066 5267 6704 Size: 197 926 983 1729 240 171 201 1437 Cuts at: 6704 7056 8797 9698 9960 820 Size: 352 1741 901 262 1553 Fragments arranged by size: 1741 1729 1553 1437 983 926 901 352 262 240 201 197 171 SnaBI TAC'GTA Cuts at: 493 8484 493 Size: 7991 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10693 SphI G_CATG'C Cuts at: 1132 8051 1132 Size: 6919 3774 SspI AAT'ATT Cuts at: 5 52 8617 5 Size: 47 8565 2081 Fragments arranged by size: 8565 2081 47 StuI AGG'CCT Cuts at: 1517 5009 5489 6353 1517 Size: 3492 480 864 5857 Fragments arranged by size: 5857 3492 864 480 StyI C'CwwG_G Cuts at: 513 1065 2569 3288 3687 5637 6827 7255 7470 Size: 552 1504 719 399 1950 1190 428 215 Cuts at: 7470 7533 8132 513 Size: 63 599 3074 Fragments arranged by size: 3074 1950 1504 1190 719 599 552 428 399 215 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2779 2891 4432 8720 8737 8890 Size: 1255 549 650 112 1541 4288 17 153 Cuts at: 8890 9075 9520 325 Size: 185 445 1498 Fragments arranged by size: 4288 1541 1498 1255 650 549 445 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3363 3852 6078 Size: 274 80 33 51 1443 1386 489 2226 Cuts at: 6078 8939 10651 96 Size: 2861 1712 138 Fragments arranged by size: 2861 2226 1712 1443 1386 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3383 3415 4126 4158 4333 4365 5211 5243 10653 Size: 32 711 32 175 32 846 32 5410 Cuts at: 10653 10685 3383 Size: 32 3391 Fragments arranged by size: 5410 3391 846 711 175 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2301 5441 6441 1316 Size: 985 3140 1000 5568 Fragments arranged by size: 5568 3140 1000 985 XbaI T'CTAG_A Cuts at: 6841 8029 6841 Size: 1188 9505 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2082 3240 4426 6307 7540 2082 Size: 1158 1186 1881 1233 5235 Fragments arranged by size: 5235 1881 1233 1186 1158 XhoI C'TCGA_G Cuts at: 1943 2926 7056 1943 Size: 983 4130 5580 Fragments arranged by size: 5580 4130 983 XmaI C'CCGG_G Cuts at: 1870 8018 1870 Size: 6148 4545 XmnI GAAnn'nnTTC Cuts at: 3165 4481 6248 8822 3165 Size: 1316 1767 2574 5036 Fragments arranged by size: 5036 2574 1767 1316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8501 276 Size: 53 83 186 936 6967 2468 Fragments arranged by size: 6967 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI PsrI SacII SfiI SrfI SwaI