(Circular) (Six-base) MAPSORT of: pFN21AB0067.seq Check: 6897 from: 1 to: 12031 pFN21AB0067 12031 bp With 182 enzymes: SgfI * May 19, 2009 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6921 7130 8248 6921 Size: 209 1118 10704 Fragments arranged by size: 10704 1118 209 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5475 9841 278 Size: 53 83 186 936 3939 4366 2468 Fragments arranged by size: 4366 3939 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6954 7116 9374 1937 Size: 5017 162 2258 4594 Fragments arranged by size: 5017 4594 2258 162 Acc65I G'GTAC_C Cuts at: 9352 9352 Size: 12031 AclI AA'CG_TT Cuts at: 1563 3174 10158 10531 1563 Size: 1611 6984 373 3063 Fragments arranged by size: 6984 3063 1611 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2373 2940 5184 5898 6081 6606 8246 10094 Size: 741 567 2244 714 183 525 1640 1848 Cuts at: 10094 11478 11746 1632 Size: 1384 268 1917 Fragments arranged by size: 2244 1917 1848 1640 1384 741 714 567 525 268 183 AfeI AGC'GCT Cuts at: 7325 8200 7325 Size: 875 11156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11834 AflIII A'CryG_T Cuts at: 1203 3787 3921 6840 7347 10930 11907 1203 Size: 2584 134 2919 507 3583 977 1327 Fragments arranged by size: 3583 2919 2584 1327 977 507 134 AgeI A'CCGG_T Cuts at: 3761 10862 3761 Size: 7101 4930 AhdI GACnn_n'nnGTC Cuts at: 3195 5323 9167 10760 3195 Size: 2128 3844 1593 4466 Fragments arranged by size: 4466 3844 2128 1593 AleI CACnn'nnGTG Cuts at: 1166 2879 2966 7799 1166 Size: 1713 87 4833 5398 Fragments arranged by size: 5398 4833 1713 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2303 3417 7737 8673 1184 Size: 1119 1114 4320 936 4542 Fragments arranged by size: 4542 4320 1119 1114 936 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3683 3715 3683 Size: 32 11999 AlwNI CAG_nnn'CTG Cuts at: 2192 2299 3047 3076 3802 4384 4973 6865 7339 Size: 107 748 29 726 582 589 1892 474 Cuts at: 7339 7648 7918 8263 8428 8596 9047 9774 11346 Size: 309 270 345 165 168 451 727 1572 Cuts at: 11346 2192 Size: 2877 Fragments arranged by size: 2877 1892 1572 748 727 726 589 582 474 451 345 309 270 168 165 107 29 ApaI G_GGCC'C Cuts at: 2433 8037 8157 2433 Size: 5604 120 6307 Fragments arranged by size: 6307 5604 120 ApaLI G'TGCA_C Cuts at: 4335 4859 7343 10088 11244 4335 Size: 524 2484 2745 1156 5122 Fragments arranged by size: 5122 2745 2484 1156 524 ApoI r'AATT_y Cuts at: 1493 3360 4168 5367 5972 6035 9340 9566 9718 Size: 1867 808 1199 605 63 3305 226 152 Cuts at: 9718 11827 1493 Size: 2109 1697 Fragments arranged by size: 3305 2109 1867 1697 1199 808 605 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 2598 10585 160 Size: 872 1566 7987 1606 Fragments arranged by size: 7987 1606 1566 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12031 AvaI C'yCGr_G Cuts at: 1870 1943 2707 4066 5520 6122 7673 7975 8057 Size: 73 764 1359 1454 602 1551 302 82 Cuts at: 8057 9356 1870 Size: 1299 4545 Fragments arranged by size: 4545 1551 1454 1359 1299 764 602 302 82 73 BamHI G'GATC_C Cuts at: 9361 9728 9361 Size: 367 11664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2702 4363 4474 6045 7828 Size: 325 217 627 915 1661 111 1571 1783 Cuts at: 7828 8074 9352 10807 618 Size: 246 1278 1455 1842 Fragments arranged by size: 1842 1783 1661 1571 1455 1278 915 627 325 246 217 111 BanII G_rGCy'C Cuts at: 1391 2369 2433 4075 4277 5028 5055 5454 7295 Size: 978 64 1642 202 751 27 399 1841 Cuts at: 7295 8037 8157 8951 9350 1391 Size: 742 120 794 399 4072 Fragments arranged by size: 4072 1841 1642 978 794 751 742 399 399 202 120 64 27 BbeI G_GCGC'C Cuts at: 2706 4367 6049 8078 2706 Size: 1661 1682 2029 6659 Fragments arranged by size: 6659 2029 1682 1661 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4677 5003 6027 6594 6889 928 Size: 971 2778 326 1024 567 295 6070 Fragments arranged by size: 6070 2778 1024 971 567 326 295 BbvCI CC'TCA_GC Cuts at: 3725 4314 7638 7663 8244 3725 Size: 589 3324 25 581 7512 Fragments arranged by size: 7512 3324 589 581 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2732 4840 4874 9203 9237 10222 Size: 34 761 34 2108 34 4329 34 985 Cuts at: 10222 10256 1903 Size: 34 3678 Fragments arranged by size: 4329 3678 2108 985 761 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9096 9923 11133 9096 Size: 827 1210 9994 Fragments arranged by size: 9994 1210 827 BclI T'GATC_A Cuts at: 1548 6472 1548 Size: 4924 7107 BfrBI ATG'CAT Cuts at: 5875 5875 Size: 12031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2304 4070 6496 Size: 107 122 71 631 605 632 1766 2426 Cuts at: 6496 8059 8141 8441 8666 10642 136 Size: 1563 82 300 225 1976 1525 Fragments arranged by size: 2426 1976 1766 1563 1525 632 631 605 300 225 122 107 82 71 BglII A'GATC_T Cuts at: 12026 12026 Size: 12031 BlpI GC'TnA_GC Cuts at: 4074 6971 7313 9448 4074 Size: 2897 342 2135 6657 Fragments arranged by size: 6657 2897 2135 342 Bme1580I G_kGCm'C Cuts at: 1790 2433 4339 4725 4863 6414 7347 7527 8013 Size: 643 1906 386 138 1551 933 180 486 Cuts at: 8013 8037 8157 8688 10092 11248 1790 Size: 24 120 531 1404 1156 2573 Fragments arranged by size: 2573 1906 1551 1404 1156 933 643 531 486 386 180 138 120 24 BmgBI CAC'GTC Cuts at: 1318 2508 6244 9015 9130 1318 Size: 1190 3736 2771 115 4219 Fragments arranged by size: 4219 3736 2771 1190 115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2823 2884 4719 Size: 416 53 83 399 24 1406 61 1835 Cuts at: 4719 4796 6014 7039 9061 9762 10720 442 Size: 77 1218 1025 2022 701 958 1753 Fragments arranged by size: 2022 1835 1753 1406 1218 1025 958 701 416 399 83 77 61 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 7057 2069 Size: 4988 7043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2673 2874 2898 3333 4527 4595 8351 8756 Size: 1434 201 24 435 1194 68 3756 405 Cuts at: 8756 9024 10691 1239 Size: 268 1667 2579 Fragments arranged by size: 3756 2579 1667 1434 1194 435 405 268 201 68 24 Bpu10I CC'TnA_GC Cuts at: 3725 4314 7638 7663 8244 3725 Size: 589 3324 25 581 7512 Fragments arranged by size: 7512 3324 589 581 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2298 4143 8691 10156 11021 11319 2298 Size: 1845 4548 1465 865 298 3010 Fragments arranged by size: 4548 3010 1845 1465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2720 3351 4102 4997 9042 10694 882 Size: 617 1221 631 751 895 4045 1652 2219 Fragments arranged by size: 4045 2219 1652 1221 895 751 631 617 BsaAI yAC'GTr Cuts at: 493 1204 5464 7144 8329 9822 493 Size: 711 4260 1680 1185 1493 2702 Fragments arranged by size: 4260 2702 1680 1493 1185 711 BsaBI GATnn'nnATC Cuts at: 12025 12025 Size: 12031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2703 4364 4853 5472 Size: 53 83 186 936 1170 1661 489 619 Cuts at: 5472 6046 6999 7709 8075 9838 10220 10855 275 Size: 574 953 710 366 1763 382 635 1451 Fragments arranged by size: 1763 1661 1451 1170 953 936 710 635 619 574 489 382 366 186 83 53 BsaWI w'CCGG_w Cuts at: 3761 10464 10862 11136 11283 11694 3761 Size: 6703 398 274 147 411 4098 Fragments arranged by size: 6703 4098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3425 3455 3683 3713 3730 Size: 30 165 30 2022 30 228 30 17 Cuts at: 3730 3760 4003 4033 4561 4591 4693 4723 5042 Size: 30 243 30 528 30 102 30 319 Cuts at: 5042 5072 6321 6351 7536 7566 1178 Size: 30 1249 30 1185 30 5643 Fragments arranged by size: 5643 2022 1249 1185 528 319 243 228 165 102 30 30 30 30 30 30 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2163 2244 2558 3738 4629 4921 5087 5282 Size: 977 81 314 1180 891 292 166 195 Cuts at: 5282 5351 6701 7030 1186 Size: 69 1350 329 6187 Fragments arranged by size: 6187 1350 1180 977 891 329 314 292 195 166 81 69 BseYI C'CCAG_C Cuts at: 2396 3203 3437 4510 4644 4866 4959 5355 6827 Size: 807 234 1073 134 222 93 396 1472 Cuts at: 6827 6920 7243 7590 7694 7725 7769 7851 7902 Size: 93 323 347 104 31 44 82 51 Cuts at: 7902 8045 8085 8150 8165 8195 8234 8355 8373 Size: 143 40 65 15 30 39 121 18 Cuts at: 8373 8391 8414 8669 8839 8952 9072 9111 9234 Size: 18 23 255 170 113 120 39 123 Cuts at: 9234 9248 11234 11730 2396 Size: 14 1986 496 2697 Fragments arranged by size: 2697 1986 1472 1073 807 496 396 347 323 255 234 222 170 143 134 123 121 120 113 104 93 93 82 65 51 44 40 39 39 31 30 23 18 18 15 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 3253 4251 4547 5876 6899 7073 Size: 573 573 944 998 296 1329 1023 174 Cuts at: 7073 7147 7430 8192 1163 Size: 74 283 762 5002 Fragments arranged by size: 5002 1329 1023 998 944 762 573 573 296 283 174 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8145 9481 10242 11270 1540 Size: 275 186 6144 1336 761 1028 2301 Fragments arranged by size: 6144 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4339 4748 4863 6710 7347 10092 10177 11248 4339 Size: 409 115 1847 637 2745 85 1071 5122 Fragments arranged by size: 5122 2745 1847 1071 637 409 115 85 BsmI GAATG_Cn' Cuts at: 2542 2679 3406 4314 6243 6760 8437 9542 9635 Size: 137 727 908 1929 517 1677 1105 93 Cuts at: 9635 2542 Size: 4938 Fragments arranged by size: 4938 1929 1677 1105 908 727 517 137 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3103 7142 9136 3103 Size: 4039 1994 5998 Fragments arranged by size: 5998 4039 1994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2433 4075 4277 4339 4725 4748 Size: 399 579 64 1642 202 62 386 23 Cuts at: 4748 4863 5028 5055 5454 6414 6710 7295 7347 Size: 115 165 27 399 960 296 585 52 Cuts at: 7347 7527 8013 8037 8157 8688 8951 9350 10092 Size: 180 486 24 120 531 263 399 742 Cuts at: 10092 10177 11248 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1642 1071 960 742 585 579 531 486 399 399 399 386 296 263 202 180 165 120 115 85 64 62 52 27 24 23 BspEI T'CCGG_A Cuts at: 11694 11694 Size: 12031 BspHI T'CATG_A Cuts at: 2628 8523 9920 11650 2628 Size: 5895 1397 1730 3009 Fragments arranged by size: 5895 3009 1730 1397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2728 3810 6921 7130 8220 8248 9386 844 Size: 1884 1082 3111 209 1090 28 1138 3489 Fragments arranged by size: 3489 3111 1884 1138 1090 1082 209 28 BsrBI CCG'CTC Cuts at: 1394 1823 4370 6544 7053 8031 9918 1394 Size: 429 2547 2174 509 978 1887 3507 Fragments arranged by size: 3507 2547 2174 1887 978 509 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6457 9231 10526 10700 65 Size: 1183 5209 2774 1295 174 1396 Fragments arranged by size: 5209 2774 1396 1295 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3761 7308 7602 7831 8660 8728 10675 Size: 110 1951 3547 294 229 829 68 1947 Cuts at: 10675 10862 1700 Size: 187 2869 Fragments arranged by size: 3547 2869 1951 1947 829 294 229 187 110 68 BsrGI T'GTAC_A Cuts at: 96 6291 96 Size: 6195 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12031 BssSI C'ACGA_G Cuts at: 4227 6280 10091 11103 4227 Size: 2053 3811 1012 5155 Fragments arranged by size: 5155 3811 2053 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 7131 7170 7197 7579 7648 8098 8212 8596 Size: 6337 39 27 382 69 450 114 384 Cuts at: 8596 8752 8875 8962 794 Size: 156 123 87 3863 Fragments arranged by size: 6337 3863 450 384 382 156 123 114 87 69 39 27 BstBI TT'CG_AA Cuts at: 10835 10835 Size: 12031 BstEII G'GTnAC_C Cuts at: 1184 4780 1184 Size: 3596 8435 BstXI CCAn_nnnn'nTGG Cuts at: 4189 4874 6004 8329 4189 Size: 685 1130 2325 7891 Fragments arranged by size: 7891 2325 1130 685 BstYI r'GATC_y Cuts at: 1972 2636 3043 5350 5733 6360 7566 9361 9728 Size: 664 407 2307 383 627 1206 1795 367 Cuts at: 9728 10113 10130 11582 11668 11680 12026 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2307 1977 1795 1452 1206 664 627 407 385 383 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3510 4518 3510 Size: 1008 11023 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 4218 4587 5400 6876 7797 Size: 552 939 351 1863 369 813 1476 921 Cuts at: 7797 8145 8453 8784 9059 9098 9174 9294 513 Size: 348 308 331 275 39 76 120 3250 Fragments arranged by size: 3250 1863 1476 939 921 813 552 369 351 348 331 308 275 120 76 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4433 4821 5688 7456 8498 487 Size: 683 56 3207 388 867 1768 1042 4020 Fragments arranged by size: 4020 3207 1768 1042 867 683 388 56 BtsI GCAGTG_nn' Cuts at: 3036 4559 6708 7159 7498 8069 9547 10341 10361 Size: 1523 2149 451 339 571 1478 794 20 Cuts at: 10361 3036 Size: 4706 Fragments arranged by size: 4706 2149 1523 1478 794 571 451 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5127 5162 5616 5651 5842 5877 529 Size: 35 4563 35 454 35 191 35 6683 Fragments arranged by size: 6683 4563 454 191 35 35 35 35 DraI TTT'AAA Cuts at: 2166 5260 9335 2166 Size: 3094 4075 4862 Fragments arranged by size: 4862 4075 3094 DraIII CAC_nnn'GTG Cuts at: 7144 7170 7144 Size: 26 12005 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3282 7112 8227 11038 809 Size: 507 1966 3830 1115 2811 1802 Fragments arranged by size: 3830 2811 1966 1802 1115 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2305 2340 2584 Size: 54 1425 50 275 18 475 35 244 Cuts at: 2584 2796 3520 4290 4938 5863 6090 6326 7611 Size: 212 724 770 648 925 227 236 1285 Cuts at: 7611 8142 8331 8689 8730 8818 8998 9177 9478 Size: 531 189 358 41 88 180 179 301 Cuts at: 9478 10367 11996 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1285 925 889 770 724 648 531 475 358 301 275 244 236 227 212 189 180 179 88 54 50 43 41 35 18 EagI C'GGCC_G Cuts at: 1537 1812 8142 9478 1537 Size: 275 6330 1336 4090 Fragments arranged by size: 6330 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3660 4494 5187 6648 6678 6696 7205 9961 Size: 2307 834 693 1461 30 18 509 2756 Cuts at: 9961 1353 Size: 3423 Fragments arranged by size: 3423 2756 2307 1461 834 693 509 30 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7544 7621 10618 10992 11138 387 Size: 1064 138 5955 77 2997 374 146 1280 Fragments arranged by size: 5955 2997 1280 1064 374 146 138 77 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2373 2673 2874 2898 2940 3333 4527 Size: 393 741 300 201 24 42 393 1194 Cuts at: 4527 4595 5184 5898 6081 6606 8246 8351 8756 Size: 68 589 714 183 525 1640 105 405 Cuts at: 8756 9024 10094 10691 11478 11746 1239 Size: 268 1070 597 787 268 1524 Fragments arranged by size: 1640 1524 1194 1070 787 741 714 597 589 525 405 393 393 300 268 268 201 183 105 68 42 24 EcoNI CCTnn'n_nnAGG Cuts at: 2735 7583 2735 Size: 4848 7183 EcoO109I rG'GnC_Cy Cuts at: 2296 3032 3799 4223 4514 4970 5281 5317 7677 Size: 736 767 424 291 456 311 36 2360 Cuts at: 7677 7971 8154 8711 8922 2296 Size: 294 183 557 211 5405 Fragments arranged by size: 5405 2360 767 736 557 456 424 311 294 291 211 183 36 EcoRI G'AATT_C Cuts at: 3360 4168 5367 9340 9718 3360 Size: 808 1199 3973 378 5673 Fragments arranged by size: 5673 3973 1199 808 378 EcoRV GAT'ATC Cuts at: 2944 8124 2944 Size: 5180 6851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6913 6913 Size: 12031 FspI TGC'GCA Cuts at: 10537 10537 Size: 12031 HaeII r_GCGC'y Cuts at: 1678 2277 2706 4367 6049 7327 8078 8202 11178 Size: 599 429 1661 1682 1278 751 124 2976 Cuts at: 11178 1678 Size: 2531 Fragments arranged by size: 2976 2531 1682 1661 1278 751 599 429 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2511 2543 3181 Size: 32 135 32 509 32 398 32 638 Cuts at: 3181 3213 3234 3266 3425 3457 3547 3579 4624 Size: 32 21 32 159 32 90 32 1045 Cuts at: 4624 4656 5042 5074 5114 5146 5309 5341 7268 Size: 32 386 32 40 32 163 32 1927 Cuts at: 7268 7300 8545 8577 8785 8817 9153 9185 10672 Size: 32 1245 32 208 32 336 32 1487 Cuts at: 10672 10704 10746 10778 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1927 1487 1245 1045 638 509 398 386 336 208 163 159 135 90 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2917 5226 6955 9375 9621 669 Size: 988 281 979 2309 1729 2420 246 3079 Fragments arranged by size: 3079 2420 2309 1729 988 979 281 246 HindIII A'AGCT_T Cuts at: 748 5543 748 Size: 4795 7236 HpaI GTT'AAC Cuts at: 9621 9621 Size: 12031 KasI G'GCGC_C Cuts at: 2702 4363 6045 8074 2702 Size: 1661 1682 2029 6659 Fragments arranged by size: 6659 2029 1682 1661 KpnI G_GTAC'C Cuts at: 9356 9356 Size: 12031 MfeI C'AATT_G Cuts at: 3021 3372 9630 3021 Size: 351 6258 5422 Fragments arranged by size: 6258 5422 351 MluI A'CGCG_T Cuts at: 7347 11907 7347 Size: 4560 7471 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 3683 3726 11145 11329 1305 Size: 891 1487 43 7419 184 2007 Fragments arranged by size: 7419 2007 1487 891 184 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 2307 2342 2586 2798 3522 Size: 54 1425 343 475 35 244 212 724 Cuts at: 3522 4292 4940 6092 7613 8333 8820 9000 9179 Size: 770 648 1152 1521 720 487 180 179 Cuts at: 9179 10 Size: 2862 Fragments arranged by size: 2862 1521 1425 1152 770 724 720 648 487 475 343 244 212 180 179 54 35 MslI CAynn'nnrTG Cuts at: 518 1166 2879 2966 3786 5712 7079 7799 7964 Size: 648 1713 87 820 1926 1367 720 165 Cuts at: 7964 9989 10348 10507 518 Size: 2025 359 159 2042 Fragments arranged by size: 2042 2025 1926 1713 1367 820 720 648 359 165 159 87 MspA1I CmG'CkG Cuts at: 1606 2238 2441 3076 3502 4413 5656 6975 7760 Size: 632 203 635 426 911 1243 1319 785 Cuts at: 7760 7868 8455 8593 8884 9238 9447 10124 11272 Size: 108 587 138 291 354 209 677 1148 Cuts at: 11272 11517 1606 Size: 245 2120 Fragments arranged by size: 2120 1319 1243 1148 911 785 677 635 632 587 426 354 291 245 209 203 138 108 NaeI GCC'GGC Cuts at: 7310 7604 7833 8662 7310 Size: 294 229 829 10679 Fragments arranged by size: 10679 829 294 229 NarI GG'CG_CC Cuts at: 2703 4364 6046 8075 2703 Size: 1661 1682 2029 6659 Fragments arranged by size: 6659 2029 1682 1661 NcoI C'CATG_G Cuts at: 513 1065 2004 2355 4218 5400 6876 8784 9174 Size: 552 939 351 1863 1182 1476 1908 390 Cuts at: 9174 513 Size: 3370 Fragments arranged by size: 3370 1908 1863 1476 1182 939 552 390 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 12031 NgoMIV G'CCGG_C Cuts at: 7308 7602 7831 8660 7308 Size: 294 229 829 10679 Fragments arranged by size: 10679 829 294 229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12031 NotI GC'GGCC_GC Cuts at: 9478 9478 Size: 12031 NruI TCG'CGA Cuts at: 5673 5673 Size: 12031 NsiI A_TGCA'T Cuts at: 5877 5877 Size: 12031 NspI r_CATG'y Cuts at: 1132 1229 3791 3925 4150 4510 4603 6835 7078 Size: 97 2562 134 225 360 93 2232 243 Cuts at: 7078 7753 7948 8053 8350 8386 8404 8755 8965 Size: 675 195 105 297 36 18 351 210 Cuts at: 8965 9389 10934 1132 Size: 424 1545 2229 Fragments arranged by size: 2562 2232 2229 1545 675 424 360 351 297 243 225 210 195 134 105 97 93 36 18 PacI TTA_AT'TAA Cuts at: 2598 2598 Size: 12031 PasI CC'CwG_GG Cuts at: 1348 3574 3667 5755 7096 8503 1348 Size: 2226 93 2088 1341 1407 4876 Fragments arranged by size: 4876 2226 2088 1407 1341 93 PciI A'CATG_T Cuts at: 3787 3921 10930 3787 Size: 134 7009 4888 Fragments arranged by size: 7009 4888 134 PflMI CCAn_nnn'nTGG Cuts at: 1108 3356 7228 7339 1108 Size: 2248 3872 111 5800 Fragments arranged by size: 5800 3872 2248 111 PmeI GTTT'AAAC Cuts at: 9335 9335 Size: 12031 PmlI CAC'GTG Cuts at: 7144 7144 Size: 12031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 6172 6204 10100 10132 11613 11645 3683 Size: 32 2457 32 3896 32 1481 32 4069 Fragments arranged by size: 4069 3896 2457 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3799 4223 4970 5317 7677 7971 8711 8922 3799 Size: 424 747 347 2360 294 740 211 6908 Fragments arranged by size: 6908 2360 747 740 424 347 294 211 PshAI GACnn'nnGTC Cuts at: 1613 4126 1613 Size: 2513 9518 PsiI TTA'TAA Cuts at: 9601 9601 Size: 12031 PspOMI G'GGCC_C Cuts at: 2429 8033 8153 2429 Size: 5604 120 6307 Fragments arranged by size: 6307 5604 120 PspXI vC'TCGA_Gb Cuts at: 2707 2707 Size: 12031 PstI C_TGCA'G Cuts at: 830 3043 3507 5994 6504 6715 7584 7867 8424 Size: 2213 464 2487 510 211 869 283 557 Cuts at: 8424 8601 8880 8895 9383 830 Size: 177 279 15 488 3478 Fragments arranged by size: 3478 2487 2213 869 557 510 488 464 283 279 211 177 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12031 PvuII CAG'CTG Cuts at: 3076 3502 5656 6975 7760 8593 9238 3076 Size: 426 2154 1319 785 833 645 5869 Fragments arranged by size: 5869 2154 1319 833 785 645 426 RsrII CG'GwC_CG Cuts at: 2027 2027 Size: 12031 SacII CC_GC'GG Cuts at: 8456 8456 Size: 12031 SalI G'TCGA_C Cuts at: 1936 6953 9373 1936 Size: 5017 2420 4594 Fragments arranged by size: 5017 4594 2420 SanDI GG'GwC_CC Cuts at: 7677 7971 8922 7677 Size: 294 951 10786 Fragments arranged by size: 10786 951 294 SapI GCTCTTCn'nnn_ Cuts at: 3660 4494 7205 3660 Size: 834 2711 8486 Fragments arranged by size: 8486 2711 834 SbfI CC_TGCA'GG Cuts at: 9383 9383 Size: 12031 ScaI AGT'ACT Cuts at: 10279 11991 10279 Size: 1712 10319 SexAI A'CCwGG_T Cuts at: 2521 3888 8022 9027 2521 Size: 1367 4134 1005 5525 Fragments arranged by size: 5525 4134 1367 1005 SfcI C'TryA_G Cuts at: 826 1045 2937 3039 3503 4752 5346 5686 5990 Size: 219 1892 102 464 1249 594 340 304 Cuts at: 5990 6500 6711 7580 7863 8420 8597 8876 8891 Size: 510 211 869 283 557 177 279 15 Cuts at: 8891 9379 10514 11195 11386 826 Size: 488 1135 681 191 1471 Fragments arranged by size: 1892 1471 1249 1135 869 681 594 557 510 488 464 340 304 283 279 219 211 191 177 102 15 SfiI GGCCn_nnn'nGGCC Cuts at: 2304 8141 2304 Size: 5837 6194 SfoI GGC'GCC Cuts at: 2704 4365 6047 8076 2704 Size: 1661 1682 2029 6659 Fragments arranged by size: 6659 2029 1682 1661 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12031 SgrAI Cr'CCGG_yG Cuts at: 1700 3761 7602 1700 Size: 2061 3841 6129 Fragments arranged by size: 6129 3841 2061 SmaI CCC'GGG Cuts at: 1872 4068 6124 7675 7977 8059 9358 1872 Size: 2196 2056 1551 302 82 1299 4545 Fragments arranged by size: 4545 2196 2056 1551 1299 302 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 2313 2707 4158 8706 10135 11036 Size: 197 926 370 394 1451 4548 1429 901 Cuts at: 11036 11298 820 Size: 262 1553 Fragments arranged by size: 4548 1553 1451 1429 926 901 394 370 262 197 SnaBI TAC'GTA Cuts at: 493 9822 493 Size: 9329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12031 SphI G_CATG'C Cuts at: 1132 4150 4603 6835 7078 7948 8053 8350 8386 Size: 3018 453 2232 243 870 105 297 36 Cuts at: 8386 8404 8755 9389 1132 Size: 18 351 634 3774 Fragments arranged by size: 3774 3018 2232 870 634 453 351 297 243 105 36 18 SspI AAT'ATT Cuts at: 5 52 3953 9955 5 Size: 47 3901 6002 2081 Fragments arranged by size: 6002 3901 2081 47 StuI AGG'CCT Cuts at: 1517 2160 2565 8116 8493 8513 8984 1517 Size: 643 405 5551 377 20 471 4564 Fragments arranged by size: 5551 4564 643 471 405 377 20 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 4188 4218 5400 5590 6606 Size: 552 939 351 1833 30 1182 190 1016 Cuts at: 6606 6876 7419 8784 9174 9470 513 Size: 270 543 1365 390 296 3074 Fragments arranged by size: 3074 1833 1365 1182 1016 939 552 543 390 351 296 270 190 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2291 2574 4231 4322 4581 5747 6039 Size: 1255 711 283 1657 91 259 1166 292 Cuts at: 6039 6480 7109 8194 8209 10058 10075 10228 10413 Size: 441 629 1085 15 1849 17 153 185 Cuts at: 10413 10858 325 Size: 445 1498 Fragments arranged by size: 1849 1657 1498 1255 1166 1085 711 629 445 441 292 283 259 185 153 91 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6291 10277 11989 Size: 274 80 33 51 1443 4314 3986 1712 Cuts at: 11989 96 Size: 138 Fragments arranged by size: 4314 3986 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3028 3060 4693 4725 6321 6353 11991 12023 3028 Size: 32 1633 32 1596 32 5638 32 3036 Fragments arranged by size: 5638 3036 1633 1596 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4552 5471 5576 1316 Size: 3236 919 105 7771 Fragments arranged by size: 7771 3236 919 105 XbaI T'CTAG_A Cuts at: 9367 9367 Size: 12031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4351 6917 8791 4351 Size: 2566 1874 7591 Fragments arranged by size: 7591 2566 1874 XhoI C'TCGA_G Cuts at: 1943 2707 1943 Size: 764 11267 XmaI C'CCGG_G Cuts at: 1870 4066 6122 7673 7975 8057 9356 1870 Size: 2196 2056 1551 302 82 1299 4545 Fragments arranged by size: 4545 2196 2056 1551 1299 302 82 XmnI GAAnn'nnTTC Cuts at: 4397 5723 10160 4397 Size: 1326 4437 6268 Fragments arranged by size: 6268 4437 1326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5473 9839 276 Size: 53 83 186 936 3939 4366 2468 Fragments arranged by size: 4366 3939 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I EcoICRI FseI FspAI PfoI PsrI SacI SrfI SwaI